Conserved Protein Domain Family
SiR_like1

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cd06200: SiR_like1 
Cytochrome p450- like alpha subunits of E. coli sulfite reductase (SiR) multimerize with beta subunits to catalyze the NADPH dependent reduction of sulfite to sulfide. Beta subunits have an Fe4S4 cluster and a siroheme, while the alpha subunits (cysJ gene) are of the cytochrome p450 (CyPor) family having FAD and FMN as prosthetic groups and utilizing NADPH. Cypor (including cyt -450 reductase, nitric oxide synthase, and methionine synthase reductase) are ferredoxin reductase (FNR)-like proteins with an additional N-terminal FMN domain and a connecting sub-domain inserted within the flavin binding portion of the FNR-like domain. The connecting domain orients the N-terminal FMN domain with the C-terminal FNR domain. NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues, and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule, which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99797
View PSSM: cd06200
Aligned: 19 rows
Threshold Bit Score: 268.377
Threshold Setting Gi: 88812170
Created: 15-May-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:by homology to E coli sulfite reductase

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              #                                         
gi 2981043   511 WSLARRECLNPGSQGESTWLLDLRapa-nsaIQWRAGDLLEIVPHQapvrirewlqrhnldgqarvavegveqsl--eqa 587
gi 94314555  228 WRLVERRLLNPGSQGEAAFHLALEped-asqLAWRAGDIAEIGPCQppdvvdrllarlsldgtapvrcdsrpmtlseala 306
gi 16127293  211 WTLAERTLLNPGSPGGEAWHLRLTpp---kgAVWQAGDIVEIGPRNdpaqvaaliaaldlpaeaadtlagl--------- 278
gi 184159371 551 WTLQQRDLLNPNSLGQPAYNIELTas---heAVWQAGDIAEIQPGNsperinkflqhhhilknavvdslqvsi------e 621
gi 119877175 237 WTLLQREHLNPGSPGGPVYWLRLQppv-gvdVQWQAGDIAEIGPQHardsarawldaqgfdadavlddgqtl-------- 307
gi 160895979 578 WPLQARSLCNAGSQGAGLYVLELSppe-gvaPLWRAGALAEVLPRHgaatvqawlaaqecdgsapvqwrqqtctl--gea 654
gi 33596450  209 WRLARRTLLNPGSQGAPCYFIELTpph-gtaPQWQAGDIAEIGPRDapd------------------------------- 256
gi 120613415 588 WRLESRETLNPGSLGNPLVEITLKpavagtpLHWPAGALVDLLPRQpadavgrwlreagldgqapvqaggaperl---ad 664
gi 118161324 585 WRLARRVLLNPGSLGGELHEITLTgp---agATWEAGALAEIIGHNadgsdg---------------------------- 633
gi 118051227 220 FVLSRRRLLNPGSQGGALYQLEWTpqs-gelPPWESGDLVSLCVPAd--------------------------------- 265
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                      ####            # #            ####               
gi 2981043   588 lagrllpdsfehlvglhpqalldaliPLSVRQYSIASLQsDGDLQLIVRQEQHa---dGSLGICSGWLTEYlpLGAALTL 664
gi 94314555  307 trlllpdsqldiltgftpqrlvdalpALPHREYSIASLPaDGRLELLVRQTRHa---dGRLGLASGWLTEFarEGASIAL 383
gi 16127293  279 -rlpqdaaaldalrglplealaerltALPHREYSIASLPdDGGVELIVRLMTRa---dGAPGLGSGWLCRHapIGAAIEA 354
gi 184159371 622 kalwnkdltgeiepfanldhlleqlpTLPTREYSIASIPsQQVLRLVVRQQYDe---sGNLGLGSGWLTQHteINQNVAL 698
gi 119877175 308 larvarshlpslvppgdlpallatlqPLPHREYSIASVMaDGALDILLRRQLRa---dGTPGIGSGWLCDHavVGEPVQL 384
gi 160895979 655 laasvlpapgdiapgtapqavadalrPLLPRSYSVASLPqDGHVRLLVRQARHd----GGLGVASGWLTAHaaQGISVAM 730
gi 33596450  257 ------------------------spLHAHREYSIASLPaDGAVHLLVRQMRSa---dGALGLGSGWLTHIaqTGQAIDL 309
gi 120613415 665 vlarsvlpalplperlhaqacadalvPLAPRTYSIASLPeDGALQLLVRQERHa----QGLGIASGWLTAHapLGSIQTL 740
gi 118161324 634 ---------------------dahhaQHAPRSYSLASLPsDGELQLLVRQERHagdenQGHGVCSGWLTRFapLGAGIRL 692
gi 118051227 266 --------------------------PERARDYSIASITaDGSLQLLVRESTRa---dGSPGLASHWLCCGmaEGDVLSL 316
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 2981043   665 RLRRNAGFHLPe-----dDVPLILIGNGTGLAGLRSLLRASiaAGRKRNWLLFGERNfAHDYYCRAELEQALarGELQRL 739
gi 94314555  384 RVRTNRSFHPPe-----dGRPLILIGNGTGLAGLRAVIKARaeAGHHRNWLLFGERSaAHDNFHAEEFQAWLsqGVLQRV 458
gi 16127293  355 RVRVNRSFHAPe-----tKRPLILIGSGTGLAGLRAHLKARaaAGVRWNWLIFGERTaAHDYFHRAELEAWQasGLLDRL 429
gi 184159371 699 RIRTNESFHLId-----dNRPIICIGNGTGIAGLMSLLHTRtrHNYTENWLIFGERQrNHDFFYASTIEAWQtmGMLKRL 773
gi 119877175 385 RLRRNDNFHGVa-----aDVPLLLIGNGTGIAGLRAHLHERarDGARRTWLLFGERTaEHDFHFGEELQAMLadGTLARL 459
gi 160895979 731 RLVENRAFEPRdeadaepDAPAIFIGNGSGYAGLRGHLLARmhAGRHRNWLLFGERQrAHDGYCEAEVSGWLaaGKLQRA 810
gi 33596450  310 RVRANRNFHPPa-----dARPLILVGNGTGLAGLRALLKARrsAGHGRNWLVFGERSaAHDWFCRDELAQWRgeGWLEYV 384
gi 120613415 741 RFVENPCFGLEgt----eGRPCLFIGNGSGLAGLRSLLRARvrAGQQRNWLLFGERQrAADTLCAGEIAHWQarGCLPRL 816
gi 118161324 693 RLQANPAFAPAl-----vDVPCIYIGNGSGLAGLRAHLRARqrAGLARNWLLFGERQqAFDSVCGEELQGWLdaGHLARL 767
gi 118051227 317 TLREHRGFRLGdn----aGRPLILIGNGSGLAGLLSHIKARvqQDRNDQWLLFGERSpQHDAFCAEQLAAWVeqGRLARL 392
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
Feature 1                                                                                      
gi 2981043   740 DLAFSRDQae---kIYVQDRLHEaaDELRRWIDEGAALYVCGSLqGMAGGVDRVLReVLGEEALQRLAdEGRYRRDVY 814
gi 94314555  459 DHAWSRDGam---pRYVQDAVSAqaATIAAWVEQGASIYVCGSLqGMAAGVQDALLrILGESTLQRLTeAGSYRRDVY 533
gi 16127293  430 DLAFSRDQae---rVYVQHRLREsaDILRRWVANGAAIYVCGSLeGMSREAHTALVeILGAPMLERLTdEGRYRRDVY 504
gi 184159371 774 DLAFSRDQeq---rVYVQDIIRQnaAELINWIERGAVLYVCGSIdGMASGVDQALIhILGEEQVDELRqQGRYRRDVY 848
gi 119877175 460 DAVFSRSGga---hRYVQDRLLAeaATLRQWVDEGTTILVCGSLqGMAPAVDAVIEqVLGAEGKEALQlAGRYRRDVY 534
gi 160895979 811 DFIYSRDQar---rRYVQDALREaaEPLRDWIADGAVLYVCGSAdGMAAGVDAALAdILGATAVEDLIvEGRYRRDVY 885
gi 33596450  385 DAVFSRDApq---rRYVQDLLREraERVRDWIREGAAVYVCGSLhGMAEGVHQALAdILGPAQLQALQhAGRYRRDVY 459
gi 120613415 817 DLAFSRDAda--phRYVQDALRAamPELRRWLDDGAVVFVCGSAeGMGAGVDHVLSdALGAEGMDALIdAGRYRRDVY 892
gi 118161324 768 DRVFSRDGdevgkrQYVQDRLRAcaDELRDWLAQGAIVYVCGSLqGMAAGIDAVLQeVLGQEGLDALLaAGRYRRDVY 845
gi 118051227 393 DQAWSRNGkd---sRYVQDLLRShaEEVRQWVERGAAIYVCGSLnGMGQGVHLTLQaILGEAKVAELLgSGRYRRDVY 467

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