Conserved Protein Domain Family
SiR_like2

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cd06201: SiR_like2 
Cytochrome p450- like alpha subunits of E. coli sulfite reductase (SiR) multimerize with beta subunits to catalyze the NADPH dependent reduction of sulfite to sulfide. Beta subunits have an Fe4S4 cluster and a siroheme, while the alpha subunits (cysJ gene) are of the cytochrome p450 (CyPor) family having FAD and FMN as prosthetic groups and utilizing NADPH. Cypor (including cyt -450 reductase, nitric oxide synthase, and methionine synthase reductase) are ferredoxin reductase (FNR)-like proteins with an additional N-terminal FMN domain and a connecting sub-domain inserted within the flavin binding portion of the FNR-like domain. The connecting domain orients the N-terminal FMN domain with the C-terminal FNR domain. NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99798
View PSSM: cd06201
Aligned: 14 rows
Threshold Bit Score: 364.728
Threshold Setting Gi: 86130612
Created: 22-May-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:determined by homology to 1DDG, E coli sulfite reductase contacts to FAD

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 148547954 444 GGSPLLGLETVNRQCPQ----------AFARWGHALGd----VLGQALALVHTPqq---prTHSLELTERIAYGeqvnaP 506
gi 154247323 443 GFPQLQPLKRIDRRSAQ----------EFAQWGRDLGs----SLGHDLVLEHTAer---pkTLALELASREDYGaaigaP 505
gi 170739877 439 GWPRLMPPAWIDRQSAQ----------GFAQWGRDLGra--lGHGRDLVLAHASpv---ppTAPYTLVGRDDYDiasatP 503
gi 184215841 489 TLPRTMPLTLVERDDYGgdpramtsvlRFAPAGRDGRtgradRAGRDEHDERDGrerrqerEERLEDAGRRAAW-----W 563
gi 170788208 444 GWKTLIPFDTVDHQSTQ----------DFTRWGRALGa----ALGIELELVHQAra---paALPLTLLSRHDYGaevqsP 506
gi 118588827 446 GVAELLPMATVDRQSTQ----------QFAAWSHDLGk----AISIDLHVEHTAdl---pgLKEWECVARKDFGqayqtP 508
gi 121596372 442 GWPTLLPLERIHQQSAQ----------EFARWGRTLSq----ALGLPLQIDYQPrl---prTVALTLAARQDFPggageP 504
gi 119944057 444 GWNPLTNIDIIDKQSTQ----------AFNRWGTVVGs----LMGLDLDLIYCPvh---lnTQLLQLVDRVDYGaevnaP 506
gi 116252030 440 GWASLIPFDTVNRQSPQ----------DFARWGRLLAe----ALGLDFELKHQQia---pkTWRLPLISRRDYGasvqaT 502
gi 87120419  467 GFKQMLPLTAIDKFCQT----------SLETWIENVStc--lNQSLCLKLDKKKi-----sPFQMQLVEQSHYGdevdaP 529
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               #           ####            # #    ####  
gi 148547954 507 THVLRFNArg--------------------glPNFLAGDLVGILPPGSPIPRFYSLASGSqdGVLEICVRKHSGGMCSEF 566
gi 154247323 506 VAILRFRGpgrga--------------aagrlPAFEPGDLLGILAPGADMPRFYSLASSSrdGMVEICVRLRAGGLCSTF 571
gi 170739877 504 VAVLRLAPadg------------------getPPFRAGDLLGILAPGTEVPRSYSLATSSedGVLEICVRRHTGGLCSGF 565
gi 184215841 564 TAFARRRL------------------------PSFETGDLLGVVPPGEASPRYYSLASASsdGIVEICVRRHPHGVCSRY 619
gi 170788208 507 TVILRFELpkirliq----------rmtghgfGRFAAGDLLGVLPEGSVVPRFYSLASAHrdGFVEIVVKKHPAGLCSGQ 576
gi 118588827 509 KSILRFRAvqtkpgrf---------glsraggPSFKAGDLIGILPKGSDLPRFYSLASSAsdGFLEIAVSKHPGGLCSGQ 579
gi 121596372 505 AAILRFALpar-------------------glPRFAAGDLIGIVAPGQAVPRYYSLASGTrdGFVEICVRRMPGGVCSSH 565
gi 119944057 507 TAILRFIPaekktatff------nkirrkprlPQFSAGDLVGILPTESDGARFYSLASASknRQLEICVHQHPQGICSNY 580
gi 116252030 503 TAILRFALpkvslwq----------rltgkgfPRFEAGDLIGIVPQGSDLPRFYSLASGTkdGFLEICVRQQVGGLCSSQ 572
gi 87120419  530 VRILRFKAatdpetvlnpaideafalssdmpwPEFEVGDLVGIMPPGSDFVRYYSLASCDqdGMLEICVRKQVEGECSGF 609
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 148547954 567 LHGLdIGAQIDAFIQPNPQFRPAsGTHPVILIGAGTGIGPLAGFIRNNkARHPMHLYWGGRNPaSDFLYEPELNQYLsdr 646
gi 154247323 572 LHGLaPGDRVEAFVRANPVFRPAkGRAPLILIGAGAGIGPLAGLVRANaAGRPVHLYWGGRSPsSDFLYEHELAQHLaek 651
gi 170739877 566 LHDLpIGGSVRAFIRANPEFRPApGRAPLVLVGAGTGIGPLIGFVRANrARRPIHLYWGGRRPeSDYLYARELAEHLaag 645
gi 184215841 620 LTGLqPGDTIEAFVRPHARLRPHaGAAPVILIGAGTGIGPLIGFIRHNaARRPMHLYFGARNAnDGFPYRDELDGLVrdr 699
gi 170788208 577 LFELrPGATINAFLKRSPAFHPDhSRAPLILIGAGTGIGPLAGFVRANaRYRPIHLFFGMRHPdSDFLYAEELAEWQrdg 656
gi 118588827 580 LCDLgPCDRIAGFIQPNARFNMPrGKAPVILIGAGTGVAPLVGMVRANdALKPAYLFAGSRDPqSDQLYREEIDTFRksg 659
gi 121596372 566 LHALqAGDSVQAFIRSNPGFVLPaGRAPVLLIGAGTGVAPLAGFIRDNtRHRPMHLYYGARHPeRDFYFGTELPSWQadg 645
gi 119944057 581 LHKLqLGECIKGFIRENPTFRPRlEQSSIILIGAGTGIAPLIGFIRNNhTCHPMRLYWGGRDPnSDFLYRAELEQYLqdk 660
gi 116252030 573 LTALkPRDTVAAFVRPNPSFRPArGGKPVILIGAGAGIGPLAGFARRNrARRPMHLYFGTRHPaSDALYAEELSYWKkdg 652
gi 87120419  610 LHGLkEGDSIQAFIQKKASFRPAqDASSVIMIGAGTGMAPLQGFIKQNeKQVPYYLYWGGRLQnSDFIYEDNLSQALats 689
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 148547954 647 RLTQLHAAFSQVQe-rSYVQDRLISDaLALRRLIEKGaQVLVCGSREMAKGVMQALDe--VLAPLHLSVLIlkaqGRYRE 723
gi 154247323 652 RLTSLQTAFSRGPdgrAYVQDRIAADaPRLRELVRQGaQILVCGGRDMAEAVTRAFEp--VVRPLGLDLATlkshGRYVE 729
gi 170739877 646 RLTALRTAFSRRPg-gGYVQDRLAADaALVREQVHRGgQVLVCGGRSMAQAVRRVLDg--AVQPLGLDVTTlrsrRRYAE 722
gi 184215841 700 RLRALTTAFSRAEr-gAYVQDRLVADaRNLRELVAHGaQIMVCGGRAMADGVARAWErilADSGSSVAQLKq--qGRYVE 776
gi 170788208 657 RLRQLATACSRGRm-pRYVQDTLREEsAEIIRLVQAGaRVMVCGGRDMAAGVSAALTdilATAGLTPALLKa--eGRYAE 733
gi 118588827 660 RVRNVSYSFSRVPg-gAYVQDALRANaEELRRMVRQGaDIMICGGTSMAEGVSKALDdvlGPLGLTVQKLRa--dGRYQE 736
gi 121596372 646 RLAGLHTTFSRTPg-gGYVQEILRRDaTQVLALIAQGaLVRVCGSRAMAQGVAQVLDallGPQGQSLAQLKe--qGRYAE 722
gi 119944057 661 RLTELVTAFSRTEe-sCYVQDKVLADaPKIQALISEGaQILVCGGRPMSKCVAAAITrviSPLQITLQDLKe--qGRYLE 737
gi 116252030 653 RLTSVSTAFSRTAs-pAYVQDIVRKDgERIGKLIAAGgQVLICGSRDMAAAVAAVLAdilAAQGFNLALLKa--tGRYAE 729
gi 87120419  690 KLKQLRLAFSRSTk-pQYVQNLLTDDaKDISKRILEGaQIMVCGSKVMADGVRTSLDkilKQEQLSVSELEq--aGRYVQ 766

                 ...
Feature 1           
gi 148547954 724 DVY 726
gi 154247323 730 DVY 732
gi 170739877 723 DVY 725
gi 184215841 777 DVY 779
gi 170788208 734 DVY 736
gi 118588827 737 DVY 739
gi 121596372 723 DLF 725
gi 119944057 738 DVY 740
gi 116252030 730 DVY 732
gi 87120419  767 DVY 769

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