2QTL


Conserved Protein Domain Family
methionine_synthase_red

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cd06203: methionine_synthase_red 
Click on image for an interactive view with Cn3D
Human methionine synthase reductase (MSR) restores methionine sythase which is responsible for the regeneration of methionine from homocysteine, as well as the coversion of methyltetrahydrofolate to tetrahydrofolate. In MSR, electrons are transferred from NADPH to FAD to FMN to cob(II)alamin. MSR resembles proteins of the cytochrome p450 family including nitric oxide synthase, the alpha subunit of sulfite reductase, but contains an extended hinge region. NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. CYPORs resemble ferredoxin reductase (FNR) but have a connecting subdomain inserted within the flavin binding region, which helps orient the FMN binding doamin with the FNR module. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99800
View PSSM: cd06203
Aligned: 13 rows
Threshold Bit Score: 456.013
Threshold Setting Gi: 146104587
Created: 15-May-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
2QTL_A        118 PISKAVQLTTnda-----ikTTLLVELDIsn-tdFSYQPGDAFSVICPNsdSEVQSLLQRLQLed--krEHXVLLKi--- 186  human
AAH97171      294 PISKAVQLTQdda-----vkTAFLLELDIsqqqeQVYQPGDAFDILCPNraSEVEELLLKLDLqm--qkNCTVQVNl--- 363  zebrafish
XP_426057     273 PVTKASQLTReda-----vkTALLLELDIad-taFDYQPGDAFCVICPNnvNEVEELLHILGLse--kgENFVCIKv--- 341  chicken
XP_001632309  185 TIVGARRLTAdda-----mkKALEITLDIsq-sgWEYEAGNSFNIYCPNdeGEVDAIIERLGLts--maQVPYDIQv--- 253  starlet sea a...
XP_001898578  117 TSLEIQKPKQe---------IVVDLEHDAe----ICYEPGDAFYFVVPNtrEEVDFILERIGFleaadrKLVVTVK---- 179  agent of lymp...
XP_001847457   59 KISNHKIIAQged-----ikTVYDVVLTTasevdYRWTPGDTVGILTRNsdEDVDSLVDHLELqs--iqHKLYRVEv--- 128  southern hous...
XP_001689322   57 CVSNLTTIVEged-----vkTVHDLTLSYppdtgHRHWPGDTIGILSYNtdADVEFLLARLNLls--kgDVVCKVGi--- 126  Anopheles gam...
XP_321281      54 SILHYRVLAEgdd-----vkTVYEVAIKLppnmeEEYFPGDAIGILTYNlaSEVDYVLDRLHLle--saDQTYEVKl--- 123  Anopheles gam...
XP_647335      59 TIESAKYMTIvdqestidikRVIEMTLNLgn-teLSYQPGDYISVFAPNthNHVISLIKRLSLd----eNKLIKIEsidk 133  Dictyostelium...
NP_495978     287 PFSKKIKTKRm---------ITVDFGDHAa---eLQYEPGDAIYFCVPNpaLEVNFILKRCGVld--iaDQQCELSi--- 349  nematode
XP_966846      39 GVKSARKLAEgs-------kTVYKLGLDLeg-vgVDFMPGDTIGVLPRNdeSLVEKIFIRLGVea--qaDQPCELGv--- 105  red flour beetle
AAL14026       66 VLVSADKATEt--------kRVVELTLESt----FDYQPGDTIGILPANklEQVESLLHRLELld--qaDTTCHLKl--- 128  fruit fly
XP_001469869 1021 VVQSWKLLTCpt-------vRHPVVQLDFsvsmgTQWAPGQTIAVVPSNstAQVEALLRLFHLt----rDEPFLPPaiag 1089 Leishmania in...
Feature 1                                                                                         
2QTL_A        187 ----KADTKKKgatLPQHIPa-gXSLQFIFTWCLEIRa-IPKKAFLRALVDytsdsaEKRRLQELCskQGAADYSRFVRD 260  human
AAH97171      364 ----LKNTSKKaakVPLHIPq-nGSLQFILTWCLEIRs-TPKKAFLRALVDftqnasEKRRLLELCskQGSADYNSFVRD 437  zebrafish
XP_426057     342 ----KQDTKKKgatRPQHIPe-rSTLKFILTWCLELRa-IPKKAFLRALVEctsdagEKRRLQELCskQGASDYTHFIRE 415  chicken
XP_001632309  254 ----MSGTKKKtasIPNYIPq-pYTIKESLLTCLDIRm-VPRKAFLRTLVEfthdnnEQRRLQELCskQGADEYCRFVRE 327  starlet sea a...
XP_001898578  180 ----SQNAVKNt-aIPPYIPe-vSTLRYIFTWCLDIRr-SPGRPLLRVLAEcteseyQKRRILELCsaQGVEEFTKFVRQ 252  agent of lymp...
XP_001847457  129 ----DPATKKKgakVPVYIPk-lVPLRKLFAECLDLKs-IPKKLFIRALLDfteddaEKRLLSVLCs-KDSSAFDDVVLK 201  southern hous...
XP_001689322  127 ----DKKTTKKaakVPAFVPa-vVSYRRLIKECLDLHa-VPKKLLICSLTPfttdddDRRFLEILCskEGNAAYEQTVQK 200  Anopheles gam...
XP_321281     124 ----AKPVKKKnpeLPHYVPk-yVTPRRLLSECLDIRi-TPRKGLLQAMASctadecEKRLLEILAskEGSNLYNELILK 197  Anopheles gam...
XP_647335     134 -tfeLPNHLKK---VNENFNnksSTIYNVLRDLVDITg-IPSKKLLKLLSEnssdsnDKLELNKLSsiEGKEEYAQMINN 208  Dictyostelium...
NP_495978     350 ----NPKTEKInaqIPGHVHk-iTTLRHMFTTCLDIRr-APGRPLIRVLAEstsdpnEKRRLLELCsaQGMKDFTDFVRT 423  nematode
XP_966846     106 ----SPNSGKKnavVPKHLPv-rSTLRTIFLNHVDLHt-PPKKLFLKSLLGfat-gsDASRLTSLSs-PQSPDYSDFIAS 177  red flour beetle
AAL14026      129 ----VFNCANKnakLPAHIPa-tTTPREILTHCLSLNf-VPQKQLLSALAGftsddkERCFLSCLSskQATEYYQSLILE 202  fruit fly
XP_001469869 1090 pgvsVFPTSPLy--EAVDFP---VSCGTVLMRYVELRvtGIHKPLFELLAR------PCASEEAKA--AVQTMYAELLAD 1156 Leishmania in...
Feature 1                                            ####                ##                ####   
2QTL_A        261 AXa-cLLDLLLAFPSCQPP---LSLLLEHLPKLQPRPYSCASSSlfh--pgkLHFVFNIVEFlstattevlrkGVCTGWL 334  human
AAH97171      438 SNv-cVLDLLRAFPSCCPP---LSLLFEHLPKLQPRAYSAASSSlqh--pgkVHLVFNVVEFparpe-hparkGLCTGWL 510  zebrafish
XP_426057     416 SNv-cLLDLLHAFPSCKPS---LNLLIEHLPKLQARSYSVSSSNlyq--pgkLCFVFNIVEFpacptrpvlrkGVCTGWL 489  chicken
XP_001632309  328 PSl-sLLELLTAFPSCFPP---FERLLEHLPRLSPRPYSISSSPlek--pgqLNFVFNVVKFgefedirksrkGVCTSWL 401  starlet sea a...
XP_001898578  253 AGl-sLVDFLLNFPSCRPT---VERLIELLPRLLPRPYSVSCIRelw--gkrIRFIFSLLHFtategrcfhrfGLSSGWL 326  agent of lymp...
XP_001847457  202 TGm-sFLTLLQNVPRCKPP---VSVLIEHLPRLMPRPYSIANAHgeg---gkVRILFSISEAc---------pGLTTSFL 265  southern hous...
XP_001689322  201 GKg--IISLLQLVPSCRPT---VALLIEHLPRLMPRPYSIANAYregaspttVRFIFSHNAEn---------pGITTSHL 266  Anopheles gam...
XP_321281     198 NEm-nFLHVLKYVATCRPP---LAMLIEHLPRLQARPYSIASYGre----nhIRIAFAMLNDgq--------vGITTHML 261  Anopheles gam...
XP_647335     209 RVt--LLCLLDKYKSSNPP---FNHLLELINPLAPRDYSISSSPlsme-kgtLSFVFSVVDFklsstpsqrilGHCTSWL 282  Dictyostelium...
NP_495978     424 PGl-sLADMLFAFPNVKPP---VDRLIELLPRLIPRPYSMSSYEnr-----kARLIYSEMEFpatdgrrhsrkGLATDWL 494  nematode
XP_966846     178 NPs--LLNVLETFPSLTPP---ISLLLEHLPPLQPRPYSICSSP--------LSHTLEITFDv---------tGLCTKFM 235  red flour beetle
AAL14026      203 QGl-lFIDILEVCVNCRPP---LAFLAEHLPRLLPRPYSIANSPleas-nreLRVIYSLLSLk---------pGVTTSML 268  fruit fly
XP_001469869 1157 RTprdLCEVLEAVGAAAHSlppFRHIVENLSLLQPRQYSICSSHrad--attLSICFKVVEK-----------GLCTRWM 1223 Leishmania in...
Feature 1                                                               #                         
2QTL_A        335 ALLvasvlqpnih-----------------------ashedsgkalAPKISIFPRTTNSFHLPdd-------psIPIIMV 384  human
AAH97171      511 VDHvssilephgta----------------------laserpgtsaLPKVHIRARPSSTFHLPsd-------pcVPVVMV 561  zebrafish
XP_426057     490 AELvapllhtnkd-----------------------tldikrgsspTEKISIFARPNNTFHLPsd-------psVPFVMV 539  chicken
XP_001632309  402 EGLtgsmiteegnkang----------------ilkiselsilsspYVQVPVSARSNSDFKLPpd-------srIPVIMI 458  starlet sea a...
XP_001898578  327 STLk-----------------------------------------kGQQVVMLLKESSRFRLPppvhssfdlarTPLIMV 365  agent of lymp...
XP_001847457  266 RSCcd----------------------------------------tNDPVFLYFRSTTHFSYKedd------llKDIVMI 299  southern hous...
XP_001689322  267 RALg-----------------------------------------kGATVHFYFRQSSAFVYEqrd------lrRNIIMV 299  Anopheles gam...
XP_321281     262 ESKll----------------------------------------hPGKWDKYLYMYLRQLKPvfnyr-eedleRNIIMI 300  Anopheles gam...
XP_647335     283 EKTgiqnnklssspssn---------------lenslndlnindsnEIRIPFILKPSPHFHLPpvd-----qlnRPIIMI 342  Dictyostelium...
NP_495978     495 NSLr-----------------------------------------iGDKVQVLGKEPARFRLPplgmtknsagkLPLLMV 533  nematode
XP_966846     236 EKQis----------------------------------------sKNQIAFYFRKPGLFRLPnl--------aNPVIMI 267  red flour beetle
AAL14026      269 EAAqqss------------------------------------pehSKKVVIYPRMSNLFRYTerd------vgRNQILI 306  fruit fly
XP_001469869 1224 YEQclsaaglplvsvasaapthaegctapsavrqpflsntaivakvRVVIPFEIRSASVFRMPrd-------plVPMILV 1296 Leishmania in...
Feature 1                                                                                         
2QTL_A        385 GPGTGIAPFIGFLQHREklqe------------------------qhpdGNFGAMWLFFGCRHkdRDYLFRKELRHFLkh 440  human
AAH97171      562 GPGTGVAPFIGFLQQREkere------------------------anqeATFGEMWLFFGCRHkdKDFLFREELEKFVhn 617  zebrafish
XP_426057     540 GPGTGIAPFIGFLQHRQklre------------------------ehteWEFGETWLFFGCRHqdRDYLFKDELRCFHen 595  chicken
XP_001632309  459 GPGTGVAPFIGFLHHRRkqmel-----------------------aedlTGFGEMWLFFGCRHreKDFLYQKELESFVet 515  starlet sea a...
XP_001898578  366 CTGTALAPFLSFLQKLAfle---------------------------aeKYKVRRELYFGIRNvkNDCIHYEDIMRSVde 418  agent of lymp...
XP_001847457  300 GVGTGIAPYLGILEHRRnsk---------------------------eaGSLGKAWLFAGFRHeeTSYLCRDELSRCLkd 352  southern hous...
XP_001689322  300 GTGTGMSPYLSFLQLRAdal--------------------------aqgETLGRAELIVGFRYrdRGYLCKAEVEEYLet 353  Anopheles gam...
XP_321281     301 GPGTGVTPYIGFLEYRKqakga-----------------------strkTKMGSAWLLTSCRYqdRNYLYEHELKGFMqa 357  Anopheles gam...
XP_647335     343 GPGTGVAPFIGFLQHLSktts-------------------------tttTPNPNTWLFFGCRSekRDFLYKDTLESMLkd 397  Dictyostelium...
NP_495978     534 GPGTGVSVFLSFLHFLRklkqd-----------------------spsdFVDVPRVLFFGCRDssVDAIYMSELEMFVse 590  nematode
XP_966846     268 ATGTGIAPFRGFLQHWKl------------------------------aNEFGDVWLFYGCRFrdRDFLFKDEINELMce 317  red flour beetle
AAL14026      307 AVGTGLAPFLGFLAHKEelik------------------------qqpqQPSGQSWLYIGAKTp-EAVLKREKLMEWQes 361  fruit fly
XP_001469869 1297 GSGTGIAPFRAFLQEREaclterqlpsgctcpkgklcgcscekatrhgqAVCGTIDVFFGCRRryEDYLFRDELSHWKds 1376 Leishmania in...
Feature 1                                                                                         
2QTL_A        441 GILTHLKVSFSRDApvgee------eapaKYVQDNIQlhgQQVARILLQEn------gHIYVCGDAKnMAKDVHDALVQI 508  human
AAH97171      618 GTLSHLIVCFSRDEpdaae-----tvnrpTYVQHNLMlhaKNLARLLLEDk------gCLYVCGDAKnMAKDVNDTLLEI 686  zebrafish
XP_426057     596 GTLTHLKVCFSRDSstte-------vaqpKYVQDVIRlyaKEVARVLLKEs------gYFYVCGDKKhMADDVSDAVLDV 662  chicken
XP_001632309  516 GTLSRLFTSFSRDQgftvs------lstpRYVQDNLKlqgDKIADLVLNDg------aVVYVCGDAKnMASNVFECFCEI 583  starlet sea a...
XP_001898578  419 NILSHVSLCESQPYndi---------sskKYVQDALQergGKISDILSRKnkdenhpaIIFVCGSSKqMLKHVADVFVHI 489  agent of lymp...
XP_001847457  353 NILNDLSVAFSRDPna-----------hcRYVQDQLEankERLGRTLCDSd------gKLYVCGDGKmMLPQVEEKIGKI 415  southern hous...
XP_001689322  354 GALDACYEAFSRDPna-----------rhKYVQSQMKengKKIVENIHNPh------aTLYVCGDSKvLLPQITETVIDI 416  Anopheles gam...
XP_321281     358 GVLDRLHVASSRDEds-----------qyKYVQDIIEdrkEELVELLLDDa------tKLYLCGEGRtMLPRIQDTIVTC 420  Anopheles gam...
XP_647335     398 NTLTKLITAFSRELdtennnntaiveptcGYVQEKMIehsKQLFDMVVNQn------aFIYICGDAKgMAVGVRNSFIDI 471  Dictyostelium...
NP_495978     591 GILTDLIICESEQKg--------------ERVQDGLRkylDKVLPFLTASte-----sKIFICGDAKgMSKDVWQCFSDI 651  nematode
XP_966846     318 GIVNRLNVAFSRESds------------kKYIQSEIDkrgADFVDWVNRGa-------RIYVCGDFRtVVKDVRTCVAKN 378  red flour beetle
AAL14026      362 SLLERLRMCYSRGEs-------------pSYVQHMLEedcEDLVEFLMKSe------tVLYICADGAkISQSIAGVLSRC 422  fruit fly
XP_001469869 1377 GVVHSLAVAFSRDTndgrf------ygggCYVQDKIQecgVALMDLILQRn------aYVFVCGDADgMAKEVHRTLRQL 1444 Leishmania in...
Feature 1                                       #
2QTL_A        509 Iskevg-vekleAMKTLATLKEeKRYLQDIW 538  human
AAH97171      687 Ignelq-ldkldAMKIVAGLREdKRYLQDIW 716  zebrafish
XP_426057     663 Lsmeme-vdkleAMRTMAMLREaKRYLQDVW 692  chicken
XP_001632309  584 Iekhlc-vgkmeAIKEMTRLRKeRKYREDVW 613  starlet sea anemone
XP_001898578  490 Fsefls-kskeeAESYLRELRTnDRYVEDIW 519  agent of lymphatic filariasis
XP_001847457  416 Ladvlc-isdeqVKALIKEWKTkHKYIEDVW 445  southern house mosquito
XP_001689322  417 LaeapgaedrdaIKTLIGRLKKeGKYREDVW 447  Anopheles gambiae str. PEST
XP_321281     421 Mskvks-mpkseAADLLAEYRKtGKYLYYSM 450  Anopheles gambiae str. PEST
XP_647335     472 Vkqeln-cdekqATDIFTNWIKeKRYILDVW 501  Dictyostelium discoideum AX4
NP_495978     652 Vasdqg-ipdleAKKKLMDLKKsDQYIEDVW 681  nematode
XP_966846     379 Lvky-----gglDEEKAEDFVKgDRFIVDTW 404  red flour beetle
AAL14026      423 Lqkamh-lteeeASQLLKKLRKqDKYRVDVW 452  fruit fly
XP_001469869 1445 Iqqhlt-lsdaaAATYLERMSKdGRYLREVW 1474 Leishmania infantum JPCM5

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