1AMO,2BF4


Conserved Protein Domain Family
CYPOR

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cd06204: CYPOR 
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NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99801
View PSSM: cd06204
Aligned: 27 rows
Threshold Bit Score: 532.218
Threshold Setting Gi: 158998770
Created: 15-May-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                     #                                  
1AMO_A       214 DAKNPFLAAVTANRKLNQg--tERHLMHLELDISds-kIRYESGDHVAVYPANDsALVNQIGEILGAd---lDVIMSLNN 287 Norway rat
2BF4_A       255 DLSQPYIAPIVKSRELFSs--nDRNCIHSEFDLSgs-nIKYSTGDHLAVWPSNPlEKVEQFLSIFNLd---pETIFDLKP 328 baker's yeast
Q00141       274 SAHNPFIAPIAESRELFTv--kDRNCLHMEISIAgs-nLSYQTGDHIAVWPTNAgAEVDRFLQVFGLeg-krDSVINIKG 349 Aspergillus niger
NP_596046    264 SQANPFFSSPVRSLELFKs--gSRNCLHLELDIAds-gMRYQTGDYASICPMNPsQAVDDLLEVLGLke-krDTVIIVKP 339 fission yeast
XP_001731494 255 DARNPCPAPVAHTRELFVegaaSRSCVHVEFDISgs-gITYQHGDHLAVWPSNPePQVERFLAVLGLwe-krHTVINVKS 332 Malassezia glob...
XP_762420    265 DAKNPYNAVVKEARELFVqgtaDRTCVHIEFDIDgs-gISYQHGDHIAVWAHNPePEVDRVLAVFGLld-krTTVIDVES 342 Ustilago maydis...
XP_502404    301 NAGNPFLANVSETRELFHd--pKRSCIHVEFDVGt--nVKYTTGDHLALHIQNSdEEVERFLKVIGLwd-krHNVIKAKP 375 Yarrowia lipoly...
BAB21543     286 DAKNPYPAPLLSSRELFLa--gDRNCVFAEFGIKdt-gVRYQTGDHVGVWPMNPeTEVERILRVLGLqd-krNTVIEVTS 361 Rhodotorula minuta
EDP54340     288 QCSPVRAVDISSSRELFAs--sDRHCLHIELDLSsqpeFTYKTGDHLAVWAGNPdAEVDILVNALGLppsrhEIPISITS 365 Aspergillus fum...
ABO09628     274 NASNPMLAKITAARELFTn--tDRHCIHMEFDTTg---ARYTTGDHLAFWFQNNeEEVQRFVKALGIan--pQQPIAISV 346 Starmerella bom...
Feature 1                                                                                        
1AMO_A       288 LdeeSNKKHPFPCPTTYRTALTYYLDITNPPrTNVLYELAQYASEPs-EQEHLHKMasssgeGKELYLSWVVEarrHILA 366 Norway rat
2BF4_A       329 Ld--PTVKVPFPTPTTIGAAIKHYLEITGPVsRQLFSSLIQFAPNAd-VKEKLTLLs----kDKDQFAVEITSkyfNIAD 401 baker's yeast
Q00141       350 Id--VTAKVPIPTPTTYDAAVRYYMEVCAPVsRQFVATLAAFAPMRk-ARQRLCVWv-----AQGLFPREGHQpmlQHAQ 421 Aspergillus niger
NP_596046    340 Id--TLDKAPVLSPTTYDTVFRYYYEICGIVsRQLLSFIAPFAPTPe-SKQELEKLg----nDYDYFKKNVVDlhlNLAQ 412 fission yeast
XP_001731494 333 Ld-pQLAKVPFPVPTTYEAVARYYLDIDAHAsRQALASFAQFAPNEv-ARDELVRLg----kDRDYFRERVSDhcyKIGQ 406 Malassezia glob...
XP_762420    343 Ld-pTLAKVPFPVPTTYEAVFRHYIDITSHAsRQTLNSFAKFAPSPd-IQAKLEKVc----aNKDIFQAEIGSrllKTTE 416 Ustilago maydis...
XP_502404    376 Id--PTYKPSFPVPTTYDTVVRYYLEINGAVsRQLLAFIAPFAPTEt-AKKEALRLg----sDKNAFADEVAKhytNIAH 448 Yarrowia lipoly...
BAB21543     362 Ld-pALAKVPFPTPTTIEAVLRHYLDINAVAnRQACGALARWAPEGsaAREKLERWg----sDKEAYHNEVAEpclKLSE 436 Rhodotorula minuta
EDP54340     366 Ld--PATKVRVPTPTTPIALFRYYLEICAPVnRDTLLGLAQFAPTPa-AKDFLLQLg----qDKTAYASFLNHthlTLGR 438 Aspergillus fum...
ABO09628     347 Ld--KTSTVRIPSPTTYETIIRHFLEINGPVsRQVLSSIAPFAPSEe-VKKATQQLg----sNKELFASHVAAkkfNIAR 419 Starmerella bom...
Feature 1                                                ####              ###                   
1AMO_A       367 ILQDYPslr----------------ppIDHLCELLPRLQARYYSIASSSKvhpnsVHICAVAVEYeaksg---------r 421 Norway rat
2BF4_A       402 ALKYLSdgakw------------dtvpMQFLVESVPQMTPRYYSISSSSLsekqtVHVTSIVENFpnpelp------dap 463 baker's yeast
Q00141       422 ALQSITskpf-------------savpFSLLIEGITKLQPRYYSISSSSLvqkdkISITAVVESVrlpga--------sh 480 Aspergillus niger
NP_596046    413 VLRRVSpdapf------------tklpFSMLLENMAHMKPRYYSISSSSVvhpdkVHVTAVVDKKewtdk--------nh 472 fission yeast
XP_001731494 407 VLQLVAgdsladddi---lkstpwpipFDRVISAIPRLSPRFYSISSSPKlspdrIHITVVGLRYtppqa--------sf 475 Malassezia glob...
XP_762420    417 AMQWIAgddlqadvs----sikpwnvpFDRVISDLPRVGPRFYSISSSPKmhpksVHITAVVLRYqanks--------ap 484 Ustilago maydis...
XP_502404    449 VLSKLSggepw------------tnvpFSFLVESLPHLIPRYYSISSSSLvdkskISITAVVESLeape----------y 506 Yarrowia lipoly...
BAB21543     437 VLLAANgeepradpmdpaskitawnipFDRIISLVPRLQPRYYSISSSPKlypdsIHVTAVVLKYrpgedgnkqvaqdak 516 Rhodotorula minuta
EDP54340     439 LLQLACpdtpw------------talpLSYLIETLTPIQPRYYSISSSSVlaprkPSITVLVSCTplpadp-------tq 499 Aspergillus fum...
ABO09628     420 LLLHLSggqpw------------knvpFSFVIETIPHLQPRYYSISSSSVqspntVSITAVVERQtltgv--------dh 479 Starmerella bom...
Feature 1           ####                                                                         
1AMO_A       422 vnkGVATSWLRAKepag-------------------------------------enggRALVPMFVRKSQFRLPFkstTP 464 Norway rat
2BF4_A       464 pvvGVTTNLLRNIqlaqnnvniaetnl-----------------pvhydlngprklfaNYKLPVHVRRSNFRLPSnpsTP 526 baker's yeast
Q00141       481 mvkGVTTNYLLALkqkqngrslsrpsr------------------ldllhhgprnkydGIHVPVHVRHSNFKLPSdpsRP 542 Aspergillus niger
NP_596046    473 ifyGLTTNYLLAHcrhmhgekiphpn-------------------gleytlegprknwTGKIPMFVKKSTFRLAPp-dVP 532 fission yeast
XP_001731494 476 dtfGLASNFLSAVkmavhdetpalgdprygapl------yhlggpqgkymstdesgrkNIKVPVHVRRSNFRLPTstkVP 549 Malassezia glob...
XP_762420    485 wvhGLATNLISSIkmakngeqptgpsdpryg----------tpkylldgprgaytkdgAFRAPIHIRRSNFRLPTspkIP 554 Ustilago maydis...
XP_502404    507 aikGVATNLLLDMkikkdgvdpskskdp---------------qavhyelsgprgkfgGHKLPVHIRRSNFKLPSdpkKP 571 Yarrowia lipoly...
BAB21543     517 yvfGTATNYLLNVkmhqhnekarieqqgqtthhedtrdhgspqykldgprnkfksadgGYCVPIHVRRSNFRLPTspkIP 596 Rhodotorula minuta
EDP54340     500 sihGLTSNYLLALsaslassrhphgl--------------------typlagpnasltGGKIHVHLRRSRFKLPLmskCP 559 Aspergillus fum...
ABO09628     480 elrGVATNQILALsealvghpsmtyrl------------------qqphdftnslssqDIRVPVHIRHSLFKLPGkptVP 541 Starmerella bom...
Feature 1                                                                                        
1AMO_A       465 VIMVGPGTGIAPFMGFIQERAWLre-------------qGKEVGETLLYYGCRRsdEDYLYREELARFhk-dGALTQLNV 530 Norway rat
2BF4_A       527 VIMIGPGTGVAPFRGFIRERVAFlesqk-------kggnNVSLGKHILFYGSRNt-DDFLYQDEWPEYakklDGSFEMVV 598 baker's yeast
Q00141       543 IIMVGPGTGVAPFRGFIQERAALaa-------------kGEKVGPTVLFFGCRKsdEDFLYKDEWKTYqdqlGDNLKIIT 609 Aspergillus niger
NP_596046    533 IIMVGPGTGVAPFRGFVMERANLas-------------kGVKVAKTLLFYGCQYsdKDFLYKEEWQQYkdvlKDSFELIT 599 fission yeast
XP_001731494 550 IIMVGPGTGVAPFRSFVQERVATarkakd----klgseaLNDWADLHLYYGCRRsdEDFLYCDEWGQYakelNDKLKLRL 625 Malassezia glob...
XP_762420    555 VIMVGPGTGVAPFRSFVQDRVCSaekaleksngksaqeaLKDWGNLWLFYGCRKadEDFLYRDEWPQYaqklGGKFIMET 634 Ustilago maydis...
XP_502404    572 IIMIGPGTGLAPFRAFVMERAKQae-------------sGTDVGQQLLFFGCRNpnEDFIYKEQWAGIekelGDKFTMVT 638 Yarrowia lipoly...
BAB21543     597 VVMIGPGTGVAPFRGFIQERVALarkaia----kdgpdaLKDWGDITLFYGCRHpeVDYLYQEEFAEYekelGGKFKLFV 672 Rhodotorula minuta
EDP54340     560 LVMVAAGTGLAPFRAFIAERRQLsl-------------iGKEIGEMILFFGCRAptQDFIYKEELEELqaalGDKLRVIT 626 Aspergillus fum...
ABO09628     542 IIMVGPGTGVAPFRGFVHERASQka-------------aGKEVGKAMLFTGSRHanEDFLYRDEWKQF----SDFLDLET 604 Starmerella bom...
Feature 1                                                                                        
1AMO_A       531 AFSREQa----hKVYVQHLLKrdREHLWKLIHEGgAHIYVCGDARnMAKDVQNTFYDIVAEFGPmeh-tqAVDYVKKLMt 605 Norway rat
2BF4_A       599 AHSRLPnt---kKVYVQDKLKdyEDQVFEMINNG-AFIYVCGDAKgMAKGVSTALVGILSRGKSitt-deATELIKMLKt 673 baker's yeast
Q00141       610 AFSREGp----qKVYVQHRLRehSELVSDLLKQK-ATFYVCGDAAnMAREVNLVLGQIIAAQRGlpa-ekGEEMVKHMRr 683 Aspergillus niger
NP_596046    600 AFSREQd----hKIYVQHRLLehSDTIAKLVEEG-AAFYICGDADhMAKDVVNALASILTTVDVd-----GMKAVKALRd 669 fission yeast
XP_001731494 626 SFSREVfkpdgsKLYVQDLIWedREYLADAILNKrAYIYICGEGKsMCHDVESTLARILGEAKGvde-eaGRAELKQLKe 704 Malassezia glob...
XP_762420    635 SLSREKfkpdgsKLYVQDLIWerRKQIAEDILERkAYIYICGEAKgMAHDVEAIFGKILEEAKGsa--eeAKKEIKLLKe 712 Ustilago maydis...
XP_502404    639 AFSRVDpv---qKVYVQHRMQeyAKQINDLMQQG-AYFYVCGDASrMAREVQATLAKILSDQRGipl-ssAEQLVKSLKv 713 Yarrowia lipoly...
BAB21543     673 AFSRVPgq---kKTYVQHMISeqRELIKQTLVEKkGYAYICGDAKnMARDVEELLKEILAEAKGgtkqkeGEAEYKMLKe 749 Rhodotorula minuta
EDP54340     627 AFSREVgs---qKVYVQDRIAehAAEVIRLIDEG-ASVYICGRAG-MAREVEKTVGEAMRAAKRwse-teLNEWSRAIKr 700 Aspergillus fum...
ABO09628     605 AFSRDSs----kKVYVQHKLKerAKDVFALLNEG-AVFYVCGDAGgMSHDVHSALLEIVAQEGNlss-edADKFVRKMRs 678 Starmerella bom...
Feature 1                #
1AMO_A       606 kGRYSLDVW 614 Norway rat
2BF4_A       674 sGRYQEDVW 682 baker's yeast
Q00141       684 rGRYQEDVW 692 Aspergillus niger
NP_596046    670 dNRFFEDTW 678 fission yeast
XP_001731494 705 rNRMMLDVW 713 Malassezia globosa CBS 7966
XP_762420    713 rSRLLLDVW 721 Ustilago maydis 521
XP_502404    714 qNVYQEDVW 722 Yarrowia lipolytica CLIB122
BAB21543     750 rNRLLLDVW 758 Rhodotorula minuta
EDP54340     701 kNKWQEDVW 709 Aspergillus fumigatus A1163
ABO09628     679 rNKYQEDVW 687 Starmerella bombicola

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