Conserved Protein Domain Family
bifunctional_CYPOR

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cd06206: bifunctional_CYPOR 
These bifunctional proteins fuse N-terminal cytochrome p450 with a cytochrome p450 reductase (CYPOR). NADPH cytochrome p450 reductase serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99802
View PSSM: cd06206
Aligned: 31 rows
Threshold Bit Score: 410.496
Threshold Setting Gi: 21449376
Created: 15-May-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:determined by homology to E. coli sulfite reductase

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       #                                 
gi 117298     666 NVVASKELQQpgs--arsTRHLEIELPk----------eASYQEGDHLGVIPRNYeGIVNRVTARFGLDASqqirlea-- 731
gi 171680480  572 TIAAIRQLADtsv--gpaKYHVDVRLPp----------gCHYRAGDYLVVQGRNStESVHRTIARFSLSPAdtmtvq--- 636
gi 21449376   662 VVLENRELVRgpd--agsKRHLELRLPd----------gTSYRTGDYLSVLPQNHpDLVRRAVARLGTRAErvvtves-- 727
gi 27901545   691 LVVENTLLTQagv---paKRTIRFKLPt----------dTQYKCGDYLAILPVNPsTVVRKVMSRFDLPWDavlriekas 757
gi 78059919   679 SIVDNRELVDisvagsrsKKHIELKLPe----------aMTYRSGDYLAVLPRNAkNNVDRVLRRFRVSWDtqvvieg-- 746
gi 134100738  681 TVTEVADLTApdy--prtKRFVRLALPe----------gTAYRTADHLAVLPVHDaALVERAAGVLGVDLDtvldir--- 745
gi 159899041  668 SVLENRELVDlssplgrsKRHIELRLPn----------eLQYQAGDYLAILPQNHpSLIERACKHFGLKPEqtiilha-- 735
gi 171687609  714 LVLVNKVLAKkgl--gpeKRQIDILLPp----------gVQYRSGDYLAIQPFNPrDSIRRVLSRFGLHPDdlitvsg-- 779
gi 121703331  677 SVIENKLLTAsgv---kaKRHLELALPp----------nAEYTVGDYLHILPMNPpVTVQRAMGFFRLAEGtymkvt--- 740
gi 29827116   675 TVVANEELVSdat--glwDFSIEPPRPaaksitielpdgVTYDTGNHLAVFAKNEpVLVNRALARLGVDRDqvlrldq-- 750
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 117298     732 --eeeklaHLPLAKTVSVEELLQYVELQDPvtrtqlramaaktvc-----pphkveleallekqaYKEQVLAKr---lTM 801
gi 171680480  637 ---sskkdFLPSQPMAIEHFLRQRVELAAPitkrqlltlslhaeds----spekahllnltqdsaYEDLLTNRh----SV 705
gi 21449376   728 --saptglVPVGRALRVDELLTRCVDLSAPagagvvarl------------------aercpcppERAELAATtg--aTL 785
gi 27901545   758 pssskhisIPMDTQVSAYDLFATYVELSQPaskrdlavladaaavd----petqaelqaiasdpaRFAEISQKr---iSV 830
gi 78059919   747 --tssnprLPLGQAIGCGELFSSYVELAVPatrsqvsslaaatrc------ppekvelerlsadgFECEILGKr---tTV 815
gi 134100738  746 --akrpgrLTFDRSLTVRELLSHHVELQDPptpdgldalaal-----------npcpperaalrgLAEEARSGtadhrTL 812
gi 159899041  736 --trgaanLPIDRPISLGELLSSHVELATPatqrdlellaqknvcp----shqihlaalaadherYTTEILQKr---lSL 806
gi 171687609  780 -ttkehlkSESGGPISVFELIGTRVELANPasqrqvahlaslssg------leadklrvlasdeeYPTSVLAKr---fSV 849
gi 121703331  741 --gglstvLPIGKEISVEEALSRYVELSQPatqkilerislstp--------vltereklshqaaSSTSLQDKr---iSI 807
gi 29827116   751 -pgggrthLPVGTPVTTGLLFTEFVELQDVatrsqiqelaehtqcpwtrpqlqaytadtaeaeerYQKEILGKr---vSV 826
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                     ####                                   # #          
gi 117298     802 LELLEKYPac--eMKFSEFIALLPSIRPRYYSISSSPRvde---------------------kqASITVSVVSGEawsg- 857
gi 171680480  706 LDVLSTIPtl--aLPFGVFIDLLPPLAPRVYSISSSPLspssgt--------------lqsglvASITFDLFQSPalss- 768
gi 21449376   786 LELLERFPsc--aVDLALALELLPAPRTRLYSISSAAEe-------------------------QRAEVALTVSV----- 833
gi 27901545   831 LDLLLQYPsi--nLAIGDFVAMLPPMRVRQYSISSSPLvdp---------------------teCSITFSVLKAPslaal 887
gi 78059919   816 MDLLERFGsv--dLSLEKFLDMLPALKARQYSISSSPLwka---------------------dhVTLTVAVVDAPalsg- 871
gi 134100738  813 WDLIEDHPalrdaLSWSALLELLPATRPRQYSVSSSPAvdp---------------------rhVDLMVSVLRAParsg- 870
gi 159899041  807 LDMLEQYPss--vLDFGEFLELLPAMRVRQYSISSSSLvnp---------------------nqASLTVAVVDAPawsg- 862
gi 171687609  850 LDLLEDFPsc--kLSFASYLDMLSPLSPRQYSISSSPLaqkplpdqgsgwsseegvdlekasltATLTYDVYNSPlmshp 927
gi 121703331  808 LDLLEHAPss--tISFADFISMLQPLRARPYSISSSPLnca---------------------rsCSLTVSIVDNPeptar 864
gi 29827116   827 LNLLERFPav--eLPLAVFLEMMGPIRPRFYSISSSPLanp---------------------rhVRLTVGLLEGPalsg- 882
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ####                                                                     
gi 117298     858 --ygeyKGIASNYLAELqeGDTITCFISTPQSEFTLPkd-peTPLIMVGPGTGVAPFRGFVQARKQLke-------qGQS 927
gi 171680480  769 --hgtfNGVASSYLSSRkiGDEISCLVRPTTLPFQLPkd-isKPIAMVAAGSGIAPMRGLLQERAELmk-------qGGE 838
gi 21449376   834 ------TGVTSGYLSRVrpGDRVAVGIASPPESFRPPad-ntVPVVLIAAGTGIAPFRGFLRARAAL----------GGE 896
gi 27901545   888 tkedeyLGVASTYLSELrsGERVQLSVRLSHTGFKPPte-lsTPMIMACAGSGLAPFRGFVMDRAEKirgrrssgsmPEQ 966
gi 78059919   872 --ngrhEGVASSYLARLntGDSLSVAVRPSNARFRPPae-pdLPMILICAGSGIAPFRGFLQERALQkq-------rGEN 941
gi 134100738  871 --rgefRGAGSRHLSEVrpGDTVLARVQPCREDFRVAp---dEPLIMVAAGTGLAPFRGVIADRRERva-------nGAR 938
gi 159899041  863 --kgqfYGTGSSYLARLqvGDQIAVSLRQPHIPFRPPsa-nsTPLLMICAGTGLAPFRGFIQERVARqg-------qGEA 932
gi 171687609  928 -etksfNGVSSSYLASLqpGSRLRCFVRKTNAPFRLPsd-paTPVVMVAAGTGVAPMRAFLQERAAVagar--ggyhKSG 1003
gi 121703331  865 --etrvLGISTHYITRLkpDQRILCSIRPSNSGFTFPdnpetTPMVMICVGSGIAPFRAFVQERVCRae-------kGQK 935
gi 29827116   883 --dgryRGTCSSYIAGLesGDVFYGYVRVPSPTFAPPad-paTPLLLIGPGTGIAPLRGFLEERAHQha-------hGTQ 952
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 117298     928 LGEAHLYFGCRSPhEDYLYQEELENAqseGIITLHTAFSRMPnq--pkTYVQHVMEqdgKKLIELLDQGAHFYICGDGsq 1005
gi 171680480  839 FGEAVLFFGCRDEeKDYLYKEELERWergGVVRVVPCFSRPGgk--kgRYVTDALWeerDRMWEVVEKGGRIFTCGSAar 916
gi 21449376   897 PGPALLLFGCRGPeLDDLYAEEFAALg--DWLEVDRAYSRHPdg--evRHVQHRLWqrrDRVRELVDAGARVYLCGDAtr 972
gi 27901545   967 PAKAILYAGCRTQgKDDIHADELAEWekiGAVEVRRAYSRPSdgs-kgTHVQDLMMedkKELIDLFESGARIYVCGTPg- 1044
gi 78059919   942 VGTSLLFFGIDDPdVDFLYRDELDEWarcGVVEVMPAYSNRPee--gaRFVQDKVWlerERISALFSQGATVFVCGDGkn 1019
gi 134100738  939 QAPALCYFGCDAPdADYLHSAELRAAesaGAVAMRPAFNEAPvg--gqCFVQHRIAaeaGEVWALLESGARVLVCGDGrh 1016
gi 159899041  933 LGPNALFFGCDHPeVDLLYHEQIQAWqkaGVLEFFPAFYRQPvg--evSFVQHRLWqerQYVWSLIEQGAVIAVCGDGrs 1010
gi 171687609 1004 LGPAMLYYGCRDFeEDYLYKEELGQWekeGVVRVRAAFSRRAgeggrtGHVDELIWedrEELKGLIKQGAKILVCGSAgr 1083
gi 121703331  936 LAPGLLFIGCKSPkHDRLYGSELDQLeeaGIVTVRYAYSGLPy----rKHVQDKLWderETLVDMYKGEARFYLCGPVe- 1010
gi 29827116   953 VGLSQVFVGCRHPeHDYFYRQEMQDWeqaGIAQVHTAFSAVTgh--paRFVQDAIVgaaDTVWQAIQDGAYVYVCGDGrr 1030
                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*....
Feature 1                                                                    
gi 117298    1006 MAPAVEATLMksyadvh----------------qvseadaRLWLQQLee-kgRYAKDVW 1047
gi 171680480  917 LGRSAGEVWRriweeksg---------------qggeregEEWLEGIkg-dgRYVCDVY 959
gi 21449376   973 VGPAVEEVLGrig-------------------------pgAGWLDALra-ggRYATDVF 1005
gi 27901545  1045 VGNAVRDSIKsmflerreeirriakekgepvsdddeetafEKFLDDMkt-keRYTTDVF 1102
gi 78059919  1020 MAPAVRATLGriyqett----------------gendesaSAWIDTMerehgRYVADVF 1062
gi 134100738 1017 MAPGVREAFRgiyrertp---------------gaddasaHEWLQAMia-ggCYVEDVY 1059
gi 159899041 1011 MAPAVRETLAriyaeat----------------gseqtagMAWIAEIeq-agRYVADVF 1052
gi 171687609 1084 LGRSTAEVCLriyedefp---------------drgregaEEWLGKMke--eRYVSDVF 1125
gi 121703331 1011 LRDSVLAVARriymatvean-----------gevrgedeyQNWLAKMnk--gRFAADTF 1056
gi 29827116  1031 MAPAVREALAaiyrkht----------------gsddeaaQQWLAQLea-deRYQQDVF 1072

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