Conserved Protein Domain Family
CyPoR_like

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cd06207: CyPoR_like 
NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99803
View PSSM: cd06207
Aligned: 20 rows
Threshold Bit Score: 423.993
Threshold Setting Gi: 159471980
Created: 15-May-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:determined by homology to rat NO synthase reductase

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       #                                 
gi 170091076  676 TCTVNKRLTPley-drnVFHLEFDtag----------tgLKYSIGEALGIHGWNeeAQVLDFCAWYGVDhnrlitipv-- 742
gi 118345517  264 NVKSIKELRQnnn-dgsTLHMEIDlkn----------tnLKYKTAMNIEIFAENdpALVEKVGAHLNLDlnqrvelvvde 332
gi 145476125  268 QVVSISQLRQnqk-dgsTLQIIFDtgr----------egIEYKTAMNLGVYPENddQQILEIANYLGENldtvyslellq 336
gi 115530018  210 VVKQNVRLTAkdh-fqaIHHIAFSrvvtflegaversdpLSFEVGDALGIYCANsdAVIDSFLAQTQLSgdvvicvtpni 288
gi 145495103  207 KIIEIKELRKapspgnsTLYLSLEsq------------nIPYKAAQNIGIYPENseEYIQEICTIFNLDpkyifqiet-- 272
gi 157876011  216 EVVANRRLTSaac-eqvVHHLELRagpd-------stdaAAYEVGDALGIYCPNreELVERLLRRLQRDgaemvvvtpds 287
gi 118348220  217 RVVENELLTPady-dreTRKIEFEfds---------ndqTSYNPGDILVIHPENshQLCKDLADHLNLSlsqivritkne 286
gi 159471980  208 RLVCNQRITApdh-fqdTRHVELDleg----------sgLSYEPGDVISVLPMPseAVADAFLTRLGLDgdqwareqgqq 276
gi 145550632  175 EFTNKKRLSAeqh-dkeTYQYNFKc-------------nKLYECGDILCVKPRNqdKLVSQFLAILNLDgnkwvkleeqk 240
gi 146161695  279 PVKEIRELRQkpd-essCLHIDFDiss----------tgFKYDTAGNLGVYPENdyKSIQDFATLQGYSlddvfvlepsq 347
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 170091076  743 -------vgeeqkmhtRTVLQALQQQVDLFGrp--------------pKSFYSDLAEYATSSvdr--------------h 787
gi 118345517  333 e---fiekfkypfpspISIRSILTKFCDFQGql--------------mKKTLKDLAKLTTNEehk--------------s 381
gi 145476125  337 q----nnrakvpfpspLSVRKILKHFVDFNGqlmkntltklskissndEKLKNQLIQLTTEEgk---------------a 397
gi 115530018  289 segliqqrsqpffgrpMRLRFFLMHYFDLEAva--------------sRSFFSMLARFAGDDeevrerlfe-lassdhld 353
gi 145495103  273 -------kgnhpfptpISVHNYLKKYCDFLGpi--------------tKKQLYTLSQLVDDQqak--------------e 317
gi 157876011  288 shglvrqptrpffgrpLSLHSLLRHYFDLDAvv--------------sQEFLWMLAHGVTGEdevav---------dvre 344
gi 118348220  287 k---slqqfknpfpeyITIEQLFKQWLSISTpp--------------tRYLFKLMSYFTNDElhn--------------e 335
gi 159471980  277 gaggagaagvgpcaftARVRSLVTGCLDIGGgs--------------pRRYLFQVLLQSATAehererlsy-fatadgrd 341
gi 145550632  241 -----qnqkskkipkiISIQQLFSEFLDITSpp--------------nRYFIKLMSQYAERDihkqkltemcaqtpegle 301
gi 146161695  348 ------ertklplpspMKVSTFLSKFCDLFGpv--------------nKTILQGLADLSGNQqfi--------------a 393
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                ####               # #   
gi 170091076  788 aLLFIGSPDGsstfkkmsekdtltfadVLKKYPSArpgVEKLCELI----GDIKPRHYSIASAQsvv-gdrVDLLVVTVD 862
gi 118345517  382 kLEKLASAAGkkefdseiyqkkkclfdLLKEYQIVp-tLEQFVDLC----PRLSPRLFTISSSDvkn-peiVTVTDSLVA 455
gi 145476125  398 gFQNLVDNMKtti------------yqLIKENNIKl-nLAQFIELC----PRISPRLFTIASSNlkn-pqhVEIADSLLI 459
gi 115530018  354 dFMSYSHREKrnv------------veVLSDFRLVrppLSFLLSFM----PPMRPRIFSISSSPtfd-vseIHLTVAQLS 416
gi 145495103  318 eLKFVSSHKGreeydtnylqtrqcffnLVKKYNIRnisLQQLIELC----PLISPRYFTIASSPlkn-pnkIEIIASELL 392
gi 157876011  345 rLYELADPSNvndylqy----ahrekrNLCEVLHDfkdLHPSLELVlsfaTPMLPRYFSIASAPamdgagrFDLCVGLLD 420
gi 118348220  336 kLIEISSKEGkeeyynyvvkekrnvfeILFDFGTVqipLEYLIEGL----SLQKPREYSISSSQlsn-knrVSITIGLVK 410
gi 159471980  342 dLYRYNQREGrtl------------leVLQDFRSCappLARLLEAA----PHLRPRQFSAASSPrlrgpaaAQLLVALVS 405
gi 145550632  302 eYYSYVYREKrnv------------yeVLYDFQPCfipLEFLIDSL----KLIRERQYSISSAYdg---eqISLTVALVS 362
gi 146161695  394 dCEKWVSDSQgfeeni------reknhSIFSLSKQykiRIPLLSLIki-sNRIFPRFYTISSSNnsn-pkdLHMCISISK 465
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   ####                                                                  
gi 170091076  863 WkdsngkiryGQCTRYLAGLr-------vGQKVTVSIKPSVMKLPPnp-kQPIIMAGLGTGAAPFRAFLQHLawla---- 930
gi 118345517  456 DkledgsikhGICSKYLINFqnklkngmtGNRIRVGFKASSFILPQds-kTPIIMIGPGTGIAPFIAFCQEKealle--- 531
gi 145476125  460 DtlpngteklGLCSQYFINVqkkl-legeCVRVRVDFRESSFKLPDdp-nKSIIMVGPGTGIAPFIGFLQEReifl---- 533
gi 115530018  417 WqtplkrsrkGVYSSYLAAAv-------pGDFFTCFLWRGSLPLPSk--pVPLICVGTGTGIAPLRSLIRECaaa----- 482
gi 145495103  393 Inke----rlGLCSQFLRNVk-------vGQEIKCFLQDSAFLMPQnp-qAPILLLGIGAGLAPMRAFIQEKdefieqnr 460
gi 157876011  421 WhtplkrhrtGLCSSYLVGAa-------pGTRLTCFVWQGSLALPAt--pTPLLCIATGTGVAPIRSVLRQVagls---- 487
gi 118348220  411 YqtpfkrsivGVCSQYLKQIel------kQQKVIAWIKKGTMEMPFndptIPVILVGPGTGIAPFMSLVEQRnilierqq 484
gi 159471980  406 YvtpykrsrqGLCSAYLAGLepke--ggeEVRVAVWTERGSLRMPRsl-qTPLILVGPGTGVAPFRSFLEERcalarqah 482
gi 145550632  363 YttgknrpikGFCSNYLDNLv-------hGQQIEGKIIKGTIAFPKql-eIPIIMVGPGTGVAPFIGFIEQRvkq----- 429
gi 146161695  466 Vkdps-gtrnGQASSFFTALksn----ysGKRVRIFHQESTFKLPQdk-eKPIIMVGPGAGLAPFRAFLQEKqffee--- 536
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 170091076  931 -rngEEIGPVYYYFGSRHqaSEYLYGEEIEAfvl---dgvISRAGLAFSRDSak-------kVYIQHKMLed----sEAL 995
gi 118345517  532 kginICPGEFTLYFGCRHqnGDYIFREELEQyvk---sgiINKLYTAFSRDQgs-------kVYVQDLMIkn----eEEI 597
gi 145476125  534 qsqpQRQNEYILYFGCKHqdGDFIYKDQLREfer---sktIDRFYTAFSRDQeq-------kCYVQDLLKrn----sEEL 599
gi 115530018  483 -gsiWSDVPLLLVFGCRHegKDYIYSNEWSElskd--rlkKLTVLPAFSRDGnk-------kFYVQHQLGrh----aRRV 548
gi 145495103  461 ldqsPFKGPMTLFFGCKTm-SDYLFKEELEEyek---ngtLNQLKVAFSREGq--------qIRITNFLDv------EFL 522
gi 157876011  488 -tqgWADVPVVLVFGCRRetEDYLYREEWATlkem-galpTLRVIPAFSRDTdk-------kVYVQHKLGqh----aRLT 554
gi 118348220  485 ltqeQYSNKTFILFGCRYkqKEFYYQKFLEElhe----lnRINLFTAFSRDFds-------kVYVQHLLTkhyevfgDLI 553
gi 159471980  483 aagqPPPAPCFLFFGCRSpaADFYYQAQWEEyrrlgvldrEHGLITAFSRHTpeqqaqqgqaVYVTQRIReh----gALV 558
gi 145550632  430 --gaKNKEKTILFFGSCYenKEFYYKEFLQEs--------AITLFTAFSRDQqk-------kIYVQHRILen----rDLI 488
gi 146161695  537 skqnTQFGETVLYFGCRGkkVDYIYKSDLEDfek---skvCNKLRLAFSRDGpn-------kVYVQDLMQed----lELI 602
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 170091076  996 AQMLHDDe-----gVFYLCGPTw-------------------PVPDVYEALvnalvkyk--gsdsvkaGEYLESLKekeR 1049
gi 118345517  598 YNTFFERn-----gLIYICGQNifyqlnlftnyyiliksataMGESVVNALrkivqkfk--nideqeaHKFVENLEkekR 670
gi 145476125  600 YNQILNDk-----vVIYICGSTs-------------------MGNSVQNLIkeilstng--nwteheaEEKIQSMEkqkL 653
gi 115530018  549 AKLLDEGa------YIYVCGNSt------------------pMPKDVAMTFdeigtqcc-cdgdelrgQEYMKQLRkqgR 603
gi 145495103  523 NQLLESGg------VIYVCGSTq-------------------MGRDVQIAIenmfkqvr--kvmpylaYRKVNELEeqkI 575
gi 157876011  555 SSFLQPEgagvppgVVYVCGNAk------------------qMPKDVQHTLeqiveatvagvqdeagaAAHIRALGrvgR 616
gi 118348220  554 ANNFEKI-------KIFVCGTAk------------------yMPKQVEEGFvelltkyl--egdsekaKAIIQNLIkkrQ 606
gi 159471980  559 WRLLNGLp------PAAAAAPAta-----------------aMPAGVAAAFadvavahg--gldkeaaAAWVRQLElrgR 613
gi 145550632  489 NKIVRENaq---nvMIIVTGTSk------------------kMPTQVEQVFkec------------lgEQMLQELKrrkQ 535
gi 146161695  603 ADLFLNKq-----gTIYVCGSKa-------------------VGESVKEFIinmyqeke--gmvpylaYSKIAELEsekR 656

                  ....*.
Feature 1               
gi 170091076 1050 YVLEVY 1055
gi 118345517  671 IIKELW 676
gi 145476125  654 LIKELW 659
gi 115530018  604 YMVDSW 609
gi 145495103  576 FVKELW 581
gi 157876011  617 YQVDSW 622
gi 118348220  607 YVVEAW 612
gi 159471980  614 YFVEAW 619
gi 145550632  536 YLVECW 541
gi 146161695  657 IIMEVW 662

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