Conserved Protein Domain Family
CYPOR_like_FNR

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cd06208: CYPOR_like_FNR 
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These ferredoxin reductases are related to the NADPH cytochrome p450 reductases (CYPOR), but lack the FAD-binding region connecting sub-domain. Ferredoxin-NADP+ reductase (FNR) is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins, such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap between the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2, which then transfers two electrons and a proton to NADP+ to form NADPH. CYPOR serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases, sulfite reducatase, and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. CYPOR has a C-terminal FNR-like FAD and NAD binding module, an FMN-binding domain, and an additional connecting domain (inserted within the FAD binding region) that orients the FNR and FMN -binding domains. The C-terminal domain contains most of the NADP(H) binding residues, and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule, which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99804
View PSSM: cd06208
Aligned: 16 rows
Threshold Bit Score: 359.713
Threshold Setting Gi: 134105219
Created: 22-May-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:1GAQ: Zea mays FNR binds FAD, contacts determined at 3.5A
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  • Structure:2OK7: Plasmodium falciparum FNR binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Structure:2B5O: Synechococcus ssp. FNR binds FAD, contacts determined at 3.5A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1GAQ_A        30 NLYKPKEPYVGRCLLNTKITGdda--------pgETWHMVFSt-eGKIPYREGQSIGVIADGvdk--------------- 85
2B5O_A       115 NIYRPKTPFLGKCIENYELVDegg--------sgTVRHVTFDiseGDLRYLEGQSIGIIPPGedk--------------- 171
gi 58613455   83 NIFMPKAPYTGKVVANDVHPQtltedt--gdanwETTHLTFDh-dGKVPYLEGQSIGIIAPGpdk--------------- 144
gi 37521864   14 NIYRPASPFRGKALQNINLTPddp--------dnDVRHVVLDlsgGDLRYFEGQSIGIVPPGtda--------------- 70
gi 119507926  58 NLFKPSSPLVGTCIYNKKIVGdda--------pgDTCHVIIHh-dGKLPYLEGQSVGIIPEGtdd--------------- 113
gi 116782017  98 NKYKPKTPYIGRVLLNTKITAdda--------pgETWHMVFSt-eGELPYREGQSIGVIPTGidk--------------- 153
gi 11125362  163 NTFRPASPLICRVVSVTPVTSkdsspddshgeapQVFSIVLHh-gKQLPFVEGQGIGIMPPSraaqapvadestsqrnsv 241
gi 116695680 115 HLYSRAAPAVATVTLRERATAlda--------ddDVWHIVLDfgnQAMPILEGQSIGILPPGlda--------------- 171
gi 23450982  140 NLYTPTRPLTATVSGNYQATAdda--------ssDIHHIVLDfgaTPFPVLEGQSIGIIPPGtda--------------- 196
gi 84685011  130 NLYNLGKPIEATVQGNYRLTAeds--------daDVRHIILDfagQPMPVLEGQSIGIIPPGeta--------------- 186
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              ####                 # # # #                             #
1GAQ_A        86 ---------------ngKPHKVRLYSIASSAIGdfgdskTVSLCVKRLIYtndag--------------------eivkG 130
2B5O_A       172 ---------------ngKPHKLRLYSIASTRHGdmednkTVSLCVRQLEYqdpes-------------------getvyG 217
gi 58613455  145 --------------kgeTPAKIRLYSIASSAVGddqtskTVSLCVKRVVEldgkfanrevgedkpdkagtgypknkvyrG 210
gi 37521864   71 ---------------qgKPHKLRLYSIASSRIGdnkdgkTVSLCVKRVVYkhpet-------------------geivrG 116
gi 119507926 114 ---------------kgRPHKLRLYSIASTAAGdfgdykTLSLVVKRLVYtnekg--------------------eevrG 158
gi 116782017 154 ---------------ngKPHKLRLYSIASSALGdfgdskTVSLCVKRLVYtndqg--------------------evvkG 198
gi 11125362  242 qpdgqgqqpttvasickRRLLPRIYSIASSRDGddgcgsTLTLCVKKHIYadpvtgk----------------rdrqkdG 305
gi 116695680 172 ---------------ngRPHIERLYSVASARTGerrgtsTVALTVRRKPG-----------------------------G 207
gi 23450982  197 ---------------ngKPHLLRMYSVASPREGerphhnNLSLTVKRVTEdhdg---------------------hparG 240
gi 84685011  187 ---------------dgKPHLPRLYSVSSPRDGerpgynNLSLTVKREEQ-----------------------------G 222
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ###                                       #                                     
1GAQ_A       131 VCSNFLCDL-QPGDNVQITGPVGKEMLMPkdpnATIIMLATGTGIAPFRSFLWKMffek-------hddyKFNGLGWLFL 202
2B5O_A       218 VCSTYLCNLpVGTDDVKITGPVGKEMLLPddedATVVMLATGTGIAPFRAFLWRMfkeq-------hedyKFKGKAWLIF 290
gi 58613455  211 VCSNHICDM-SVGDDVLITGPTGAEMLLPedpkANIIMLATGTGIAPMRSYMRLLfhdkage--aadgsrKFQGLAWLFM 287
gi 37521864  117 VASNFICDL-APGDDVSITGPTGKTFLLPedpsTNLVLIATGTGIAPFRAFLRRIyde---------mdtPWQGKVWLFF 186
gi 119507926 159 VCSNFLNDI-KPGEPIKMTGPVGKEMLMPddpnATIIMLATGTGIAPFRAFMRKAfvek-------hadyQFKGKMILYL 230
gi 116782017 199 VCSNFLCDL-KPGEEVTITGPVGKEMLMPvdpnATIIMLATGTGIAPFRGYLWKMffek-------hpdyKFNGLAWLFL 270
gi 11125362  306 ICSTYICDA-KCGDEVEVTGPVGKALLLPtsteTPLVMLATGTGVAPFRSHLQALrrkllsaggpsaaqpANRPKVLLFI 384
gi 116695680 208 LCSNYLCGL-VPGETVRVVGPLGDTFLMPmeasANLIMVCTGTGVAPFRGFIQHRlrs----------mrGATGSLMLFF 276
gi 23450982  241 IASNYVCDL-KKGDKVQVTGPYGSTFLMPnhpgSSLLMICTGTGSAPMRAMTERRrrr---------meqKEGGELMLFF 310
gi 84685011  223 ICSNYVCDL-KKGDKVRVTGPFGSTFLLPsdpnAELLMICTGTGSAPFRGFTMRRqre----------mpTATEKMTLVF 291
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1GAQ_A       203 GVPTsSSLLYKEEFGKmker-apeNFRVDYAVSREQtnaagERMYIQTRMAeykEELWELLKKDntYVYMCGLKGMEKGI 281
2B5O_A       291 GVPYtANILYKDDFEKmaae-npdNFRLTYAISREQktadgGKVYVQSRVSeyaDELFEMIQKPntHVYMCGLKGMQPPI 369
gi 58613455  288 GVPYsKSLLYDDEHLAyvkn-ypdQFRFDYAVSREQtnaagQKMYIQTKMAeytDELWELMQKEntHIYMCGLKGMEAGM 366
gi 37521864  187 GMQNsNSYLYQDELAGyta---kgDFEIVEAISREQknaqgGRMYVQHRIAehaAALWELISGGntYTYICGLKGMEDGI 263
gi 119507926 231 GVPTsSSLLYRDELEEmkan-fpdQVELHYAISREMknkqgGRYYLQDSMAergEEIWQLLRKDntYVYMCGLKGMDSGI 309
gi 116782017 271 GVPTsSSLIYKEEFEKmkek-spdKIRVDFAVSREQtnekgEKMYIQTRMGeyaKELWGLFKKEntYVYMCGLKGMEKGI 349
gi 11125362  385 GARTaAAVPYMNEWRDieaqrdgnFVDIHFALSRQMknpqgKKLYIQDVVWqerEKVWKALDRDggHLYACGLKNMMVGV 464
gi 116695680 277 GGRRpVELPYCGEEDGlp----egFVDHYFCFSRQPd---aPRIYVQDGIRaagKRIRTLLQDEltHVFLCGRKGMEAGV 349
gi 23450982  311 GARApGELPYFGPLKKlp----edFIDINFAFSRVPd---ePKRYVQDRIReraDKVFRMLTDDnaFIYICGLKGMEAGV 383
gi 84685011  292 GARKpGELPYFGPLKKvp----esFLRKVFAFSRLDn---ePKHYVQDKLReekEAVAKLLKSDnaYFYICGKKEMEHGV 364
                        330       340       350
                 ....*....|....*....|....*....|....*...
Feature 1                                             #
1GAQ_A       282 DDIMvslaek-dgidwFDYKKQLKrg----dQWNVEVY 314
2B5O_A       370 DETFtaeaek-rglnwEEMRRSMKke----hRWHVEVY 402
gi 58613455  367 EECFsakaea-aglvwKEFAKSMKka----dRYHVEVY 399
gi 37521864  264 DDAFtaaa-----aakDTVWKDYRynlkqsgFWHVETY 296
gi 119507926 310 DSFMtdla-----akdGVNWADFKkqlkqqhRYNVEVY 342
gi 116782017 350 DDIItsla-----aeeGIDWNEYKrqmkkseRWNVEVY 382
gi 11125362  465 HEVLgnmae--ekglpRDHLASLLkh---qrRWHVEVY 497
gi 116695680 350 EDAFagvlrgdgasawPMVRERLLas----gRYHVETY 383
gi 23450982  384 LEAFrdic-----rahGTDWEALRpellakgRFHVETY 416
gi 84685011  365 EEALadiargeglewkPIRDKMREd-----gRYHVETY 397

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