Conserved Protein Domain Family
BenDO_FAD_NAD

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cd06209: BenDO_FAD_NAD 
Click on image for an interactive view with Cn3D
Benzoate dioxygenase reductase (BenDO) FAD/NAD binding domain. Oxygenases oxidize hydrocarbons using dioxygen as the oxidant. As a Class I bacterial dioxygenases, benzoate dioxygenase like proteins combine an [2Fe-2S] cluster containing N-terminal ferredoxin at the end fused to an FAD/NADP(P) domain. In dioxygenase FAD/NAD(P) binding domain, the reductase transfers 2 electrons from NAD(P)H to the oxygenase which insert into an aromatic substrate, an initial step in microbial aerobic degradation of aromatic rings. Flavin oxidoreductases use flavins as substrates, unlike flavoenzymes which have a flavin prosthetic group.
Statistics
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PSSM-Id: 99805
View PSSM: cd06209
Aligned: 25 rows
Threshold Bit Score: 304.902
Threshold Setting Gi: 33599717
Created: 4-Oct-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:1KRH: Acinetobacter benzoate dioxygenase reductase binds FAD, contacts determined at 4A
    View structure with Cn3D
  • Comment:FAD contacts lie primarily in N-terminal region

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                            #           ####             ### #  ####    
1KRH_A       108 HFEGTLARVENLSdsTITFDIQLddgqpdIHFLAGQYVNVTLPGTtetRSYSFSSQPgn-rLTGFVVRNVPQGKMSEYLS 186
gi 73538234  107 KFEGAIASVDKLSdsTIGFSIDLdda-gaLDFLPGQYVNVEIPGSgltRSYSFSSAPga-gHTGFVVRNVPDGRMSSYLT 184
gi 184185963 105 EVEALVTRVEVLSdsTATLHLDAssrkgqLDFLPGQYAHVQVPGTdewRSYSFACEPnesnRLQLLIRLLPDGVMSNYLR 184
gi 15597710  106 AHSGLVRELRLLSedTALLRLDAgaagrqLDFLPGQYARLQVPGSdcrRAYSFANRPnpqnHLQFLIRLLPGGAMSDYLR 185
gi 24636269  105 TMTGQMTEIDRGSssTLQFTLAIdps-skVDFLPGQYAQLRIPGTtesRAYSYSSMPgs-sHVTFLVRDVPNGKMSGYLR 182
gi 154245001 103 PFTLRIESLEQLGasVIRLKGQTlgl-kpLVFLSGQYINLKVPGTealRSYSMANPPsgerSLELLIRLLDDGAMSTYLR 181
gi 15609074  104 TGDGVVTAVELVSpsTAILRVDTsgmagaLRYRAGQFAQLQVPGTnvwRNYSYAHPAdgrgECEFIIRLLPDGVMSNYLR 183
gi 110833531 107 RFRAWVEDIHRLNdsVVELTLEVdeaerdAQFLPGMYYLLRVPNTdiwRPYSMSSTVeqlpSMRFLIRLLDDGAMSTYLE 186
gi 71906991  116 KVRAFIDDVEKLAhnVVRLTLELaeg-ewLDFRPGQFVQVKVPGLsvmRSYSPASTSadlpKIVLLIRLLPDGAMSNYLR 194
gi 114321606 118 QSEAEITELEWLSsnVVRLALTLped-qtFEFWPGQYARLRIPGTedwRSYSMSSAPgeapRVEFLIRILESGAMSDYLR 196
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          #  #                                          
1KRH_A       187 vqAKAGDKMSFTGPFGSFYLRDVk-RPVLMLAGGTGIAPFLSMLQVLEQkg--seHPVRLVFGVTQDcDLVALEQLDALq 263
gi 73538234  185 ndAQPGQRIAFSGPYGSFYLRPVq-RPVLLLAGGTGIAPFLSMLDVLASng--npHPVRMVYGVTNDiDLVELPRIDGAa 261
gi 184185963 185 drAQPGDRIRLKAPLGAFYLQQVk-RPLIFVAGGTGLAAFLGMLDQMVSnparqtQSVRLFYGVTRPqDLCELERLKGYe 263
gi 15597710  186 qgCRVGDEIRFEAPLGTFYLRQVe-RPLLLVAGGTGLSAFLGMLDELAErg--ceWPVHLYYGVRRAaDLCELQRIAGYa 262
gi 24636269  183 nqATITETFTFDGPYGAFYLREPv-RPILMLAGGTGLAPFLSMLQYMAGlqrndlPSVRLVYGVNRDdDLVGLDKLDELa 261
gi 154245001 182 erAAPGDQIACEGPRGTFYLRDGt-RPLLMVAGGTGLAPMLAMLRQIASap--tsRAMTLCFGVNTPeDLFCLDDLAELa 258
gi 15609074  184 drAQPGDHIALRCSKGSFYLRPIv-RPVILVAGGTGLSAILAMAQSLDAdv---aHPVYLLYGVERTeDLCKLDELTELr 259
gi 110833531 187 keCAIDAELEIDGPHGAFKMQQPpqGPVVLIAGGTGLAPVLSILDTLAEmrw-raHPIHLHFGVNRLsELFYLDELAARl 265
gi 71906991  195 seAARDAVLELEGPFGSFFLREKvkAPHIMIAGGTGLAPVMSMIDSIQKtsg-kkPPILLSFGCATPdSLFCLDDIELRk 273
gi 114321606 197 erAAVGDHIEVEGPHGAFYLREPe-GPLVMVAGGTGLAPMMAMLDTVRVqgs-raPKTLLSFGCATPdNLFHGDELELRc 274
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1                                                                              ####
1KRH_A       264 qKLPWFEYRTVVAHaes-qheRKGYVTGHIEYdwLNGGEVDVYLCGPVPMVEAVRSWLDTQGIQPANFLFEKFS 336
gi 73538234  262 qQLTGFEYRTCVASaes-ghdRKGYVTQHVEPewLNGGDVDIYLCGPVAMVDAVRNWLKESGVEPAGFYYEKFS 334
gi 184185963 264 qSVPGFSFHSIVMNpdadwqgASGVVTDLFEEshFNGGEVDAYLCGPPPMVDAVKSWLDQRNMENSRIYFEKFS 337
gi 15597710  263 eRLPGFRFVPVLSEadadwdgRRGYLHEHFDAarLRDEAFDLYLCGPPPMVEAVRQWLRERSLEHLRLYLEKFT 336
gi 24636269  262 tQLSGFSYITTVVDkds-aqlRRGYVTQQITNddMNGGDVDIYVCGPPPMVEAVRSWLAAEKLNPVNFYFEKFA 334
gi 154245001 259 sRLPGLEIRVAVARgdagpkwQAGYATDLLQP--GDVPGRDIYLCGPPPMTDAARAFLTAHGADPAAIFLERFV 330
gi 15609074  260 rRVGRLEVHVVVARpdpdwdgRTGLVTDLLDErmLASGDADVYLCGPVAMVDAARTWLDHNGFHRVGLYYEKFV 333
gi 110833531 266 eWLPNLNLRVTLVEphtdwqgALGYATEGVPDa-ALGPDTEVFLCGPPPMVDAAVSSLEARGIPPAHIHFESFI 338
gi 71906991  274 hWLPSLTNRISVDReaa-gslLQGNPVDALRDddVKDPDTVAYICGPQPMIEAAYRRLEALGVRHENIFAEQFT 346
gi 114321606 275 fWMSKLEVRTSVDQapegyagRIGTPVDALQAadVAAPGTTAYLCGPPPMIEAARARLIELGLPADRIFAEQFT 348

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