Conserved Protein Domain Family
MMO_FAD_NAD_binding

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cd06210: MMO_FAD_NAD_binding 
Click on image for an interactive view with Cn3D
Methane monooxygenase (MMO) reductase of methanotrophs catalyzes the NADH-dependent hydroxylation of methane to methanol. This multicomponent enzyme mediates electron transfer via a hydroxylase (MMOH), a coupling protein, and a reductase which is comprised of an N-terminal [2Fe-2S] ferredoxin domain, an FAD binding subdomain, and an NADH binding subdomain. Oxygenases oxidize hydrocarbons using dioxygen as the oxidant. Dioxygenases add both atom of oxygen to the substrate, while mono-oxygenases add one atom to the substrate and one atom to water.
Statistics
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PSSM-Id: 99806
View PSSM: cd06210
Aligned: 10 rows
Threshold Bit Score: 358.193
Threshold Setting Gi: 21239751
Created: 17-Apr-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:1TVC: Methylococcus capsulatus Methane monooxygenase binds dihydroflavin-adenine dinucleotide, contacts determined at 4A
    View structure with Cn3D
  • Comment:contacts lie primarily in the N-terminal region

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       #           ####             ####
1TVC_A        10 SFEAE-----VVGLNWVSSn--TVQFLLQKrpdec-gnrgVKFEPGQFMDLTIPGTDvSRSYSPANLPnpEGRLEFLIRV 81
gi 5102757   103 NWLAE-----ILACDRVSSn--VVRLVLQRsrpma-arisLNFVPGQFVDIEIPGTHtRRSYSMASVAe-DGQLEFIIRL 173
gi 73745631  106 NWLAE-----ILACDRVSSn--VVRLVLQPltadgaarisLNFVPGQFVDIEIPGTHtRRSYSMASVAe-DGQLEFFIRL 177
gi 74381914  106 DWQGE-----IVAVERISSn--VARLQIEPkdpetgaaisIPFVPGQYVDIEIPGSSvSRSYSMATTSt-QSRLDFLIRL 177
gi 89572587  109 CFEAE-----ITEVSQISSn--VYRLCLKRigt----nqqIKLAAGQYYELQIPGTEtWRSYSPANTEnnDGVMEFLIRV 177
gi 115358838 104 VRTAR-----IAALDAIARdtmRVELQIEPddaf---gvaAEFEAGQFAELEVPGSGlRRPYSLANTSnwDGRLEFLIRL 175
gi 21239751  106 VTATEak-tsVVSVERLSSt--VYRLVLQAldaeg-mparFDFVPGQYVEISTADSLeTRAFSLANLPndAGLLEFLIRL 181
gi 148260794  99 TREAVieriaPAGASAVSL---ALRLRPDAelg-----qaADFVPGQYMEVAIPGTDiRRAYSLANLPnwDGRVEFLIRL 170
gi 159040118 120 RREGA-----IEAVTSIAHd--TVWLRVQAapdaa-agtgVEFDPGQFVELQLPGDDrRRAYSMANTPnwDGELEFLIRL 191
gi 32470345  107 EREAE-----VMDVTMVATgtrRLLLRLQPdeq----ggaAEFEAGQFMEIQVPGSDaRRPNSLANNTnwNGDLEFFITL 177
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #   ###                        #                                #  #            
1TVC_A        82 LPEGRFSDYLRn----dARVGQVLSVKGPLGVFGLKERGM--------------APRYFVAGGTGLAPVVSMVRQMQEWT 143
gi 5102757   174 LPDGAFSKFLQt----eAKVGMRVDLRGPAGSFFLHDHGG--------------RSRVFVAGGTGLSPVLSMIRQLGKAS 235
gi 73745631  178 LPDGAFSKFLQt----eAKVGMRVDLRGPAGSFFLHDHGG--------------RSRVFVAGGTGLSPVLSMIRQLGKAS 239
gi 74381914  178 LPDGQFSNFLTm----aAKPGLTVKLRGPFGAFNLRENGF--------------RPRYFVAGGTGLSPVLSMIRYMQQEQ 239
gi 89572587  178 LPDGKFSKFLMh----dAQIGQTVHLKGPSGVFGLKENGF--------------TPRFFVAGGTGLAPILSMVRHMKAWE 239
gi 115358838 176 RAGGWFSTYLRe----rARPGDRLTVRVPMGGFGLFADSL--------------RPRWFVAGGTGLAPILSMLRRMAEYQ 237
gi 21239751  182 VPGGYYAAYLEq----rAAAGQTINVKGPFGEFVLREHELvedftlpadspargGTIAFLAGSTGLAPLASMLRELGRRG 257
gi 148260794 171 VPGGAFSTWLGt----eAKPGDALSLRGPLGRFVLDDTSP--------------RPRCLVGGGCGLAPLLSMLRHLAEFQ 232
gi 159040118 192 HEGGYFSGYLAdllagrRPLGERIVLHGPTGAFALRESGL--------------RPRWFVAGGTGLAPLLSLLRRMAEWG 257
gi 32470345  178 RPGGAFSTYLEs-----ALVGDRLNIRGPLGTFTLRENGL--------------RTFGSLVAGQGLVPLLSMLRRMADWG 238
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   #   ##                         #                                     
1TVC_A       144 apNETRIYFGVNTEpELFYIDELKSLErsMRNLTVKACVWHPSgdWEGEQGSPIDALREDLEss--dANPDIYLCGPPGM 221
gi 5102757   236 dpSPATLLFGVTNReELFYVDELKTLAqsMPTLGVRIAVVNDDggNGVDKGTVIDLLRAELEks--dAKPDIYLCGPPGM 313
gi 73745631  240 dpSPATLLFGVTNReELFYVDELKTLAqsMPTLGVRIAVVNDDggNGVDKGTVIDLLRAELEks--dAKPDIYLCGPPGM 317
gi 74381914  240 hpQEAKLFFGVTHQhELFYLEELKKLEesMPNFSAHVAVMQPDgnWQGSRGTVVDDLLKHLEgt--kAAPDIYMCGPPGM 317
gi 89572587  240 epQQSVIYFGVNTEdEIFYVDELNRLAdeMPNLTVRNCVWKATdtWNGEKGSVVDILRRDLVet--gAKPDLYLCGPPGM 317
gi 115358838 238 emGDARLFFGVNEEsELFMLDELERLRaeLPQLRVDVCVWRPGveWTGLRGTPVDALRAALAha--pVLPDVYVCGPPPL 315
gi 21239751  258 fnGECHLFFGMQDTaTMFYEKELRDIKrtLPGLTLHLALMVPSaeWEGYRGNAVAAFKEHFAas-sqIPENVYLCGPGPM 336
gi 148260794 233 dmQETHLIFGANREaELFATDEIAALAaqLPCLTVTTAIWHPEgdWSGFTGTSAEALDSWLSna--aTPPDIYVCGPPKL 310
gi 159040118 258 epHPARLLYGVTAEhDLPDLPVLAEITdaLPGFELVPCVWQPGptWTGRCGTPADVLAEDLAglrvtAAPDVYVCGPPLL 337
gi 32470345  239 emLPARLYFGARYEdELFCQEEIRQIQdkLPQLQVKICLSRPGnhWMDYRGSVVDALRDDLGsl--aTLPDLYVCGSTRL 316
                        250       260
                 ....*....|....*....|....
Feature 1                            # ##
1TVC_A       222 IDAACELVRSRGIPGeQVFFEKFL 245
gi 5102757   314 IEAAFAAAATAGVPKeQVYLEKFL 337
gi 73745631  318 IEAAFAAAATAGVPKeQVYLEKFL 341
gi 74381914  318 IDATFAAAANYGVPKdHVYVEKFL 341
gi 89572587  318 VDATYAVCTEVGIPKeHVYLEKFL 341
gi 115358838 316 VEAAREVAIAAGVPDaQFASERFT 339
gi 21239751  337 IAAALGACRELGIPDnRVHREEFV 360
gi 148260794 311 VEAVVTTARWHGVPEaQILAEQVQ 334
gi 159040118 338 VDAVQQVVRAAGLPDsHVVTERVL 361
gi 32470345  317 VQGVTELALSQGLPDsCLQFERFL 340

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