Conserved Protein Domain Family
FNR_iron_sulfur_binding_1

?
cd06215: FNR_iron_sulfur_binding_1 
Iron-sulfur binding ferredoxin reductase (FNR) proteins combine the FAD and NAD(P) binding regions of FNR with an iron-sulfur binding cluster domain. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal portion of the FAD/NAD binding domain contains most of the NADP(H) binding residues and the N-terminal sub-domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. In this ferredoxin like sub-group, the FAD/NAD sub-domains is typically fused to a C-terminal iron-sulfur binding domain. Iron-sulfur proteins play an important role in electron transfer processes and in various enzymatic reactions. The family includes plant and algal ferredoxins which act as electron carriers in photosynthesis and ferredoxins which participate in redox chains from bacteria to mammals. Ferredoxin reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
?
PSSM-Id: 99811
View PSSM: cd06215
Aligned: 35 rows
Threshold Bit Score: 267.919
Threshold Setting Gi: 87122003
Created: 23-Mar-2005
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:contacts primarily in the N-terminal region
  • Comment:based on Acinetobacter sp. Benzoate dioxygenase reductase (1KRH) and Methylococcus capsulatus Methane monooxygenase (1TVC)

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                         #                  ####                ### #   
gi 17548284   36 LVCCHVRQETHDVKSFFFRSPAGr-tFLFEPGQFITLE-LDIEGEti----nRCYTLSSSPARPh---tISITVKRVpgG 106
gi 37676069  328 LTCVEKEAIARDFVTFWLEPEHGq-lPAYLPGQHLPVE-ITVDGDti----sRRYTLSSSPSRPg---rYAISVKRIdgG 398
gi 89071919  270 LTCVKREIIAKDFVSFSFKAQKDtalAPYLPGQYLPIR-IAINGNvv----eRCYTLSSSPLEQe----YTISVKRIeqG 340
gi 62423544   47 VECVSITEVTHNVKSFELFAPWLa-rIDFEPGQYVTVRiPELELE-------RCYSIASAPFGTn---tFTLTVKRVdaG 115
gi 117618335 338 LVCVAREPLARDLETFWFEAADGeplPDYLPGQHLPIS-LDIGLGlrrerlqRRYTLSSTPERPe---rYSISVKKVggG 413
gi 45441063   34 MQVHSIVQETPDVWSLRLINHDF---YSYLPGQYALVS-IRNSDEt-----lRAYTLSSTPGLSp---fIQLTVRCLpdG 101
gi 32033385   14 LQVYSIVQEAPHVKTINFIAQDF---YPYQAGQYALVS-IKNTPHi-----tRAYSLSSTPGESr---fVSITVREIegG 81
gi 119775766 285 LRLITKEPIAKDFMGLSFEAADGkplPTFLPGQHLVFA-FDINGTrh----iRRYSLTHAPSEGn----YHIGVKRTrdG 355
gi 87122003  376 ATLVKIVEETETVKTFRFVVSQR---IAFLPGQYASFEfNNILGGaas--evRTWTLSETPNSLkgdntLDISVKRVpnG 450
gi 149196883   3 LRVSEIIEHCPTIKTFCLEPLEEy--KKYFAGQYLTVH-TGEEGDv-----lRPYSLSSSPTNSg---sYEISVKLIegG 71
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ###                                              #  #                           
gi 17548284  107 KVSNWLHDNLqPGAAIRVLGPaGEFTCARhp--------aRKYLFLSAGSGVTPLMSMSRAHHDlae-------drDIVF 171
gi 37676069  399 RVSNWLIDHLeVGDELFAQSPqGQFHIQEdk--------hQPLLLLSAGSGVTPMLSMLRYLADhdl-------veDVVF 463
gi 89071919  341 TVSNWLHDNLqVGDTIWSEKPsGQFYLEPhk--------qQNTLLLSAGSGVTPMMSMLRSLISern-------tqGLTF 405
gi 62423544  116 AVSTHLHDVLkVGDRIHVDGPyGLFSTSFhp--------aEKHLFVSGGSGISPIMSMVRSLLArpag-----tptDIVL 182
gi 117618335 414 RISHWLHQQLqPGDRLLAAAPaGEFHLGTg----------RSLLLLSAGSGVTPMLSIARTLALrge-------lgDVHF 476
gi 45441063  102 EGSRWLTQQVnVGDYLWLSDAqGEFTCANad--------dDHYLMLAAGCGVTPVISMCRALLAqrp-------qaDIRV 166
gi 32033385   82 VGSTWLNNEVkVGDQVWFSNPmGEFSCQHvl--------aDNYLLVGAGSGVTPIMSMARWLLAnrp-------kvNLTV 146
gi 119775766 356 LISNWLHDELtVGDTVLCSRPeGRFIPKAg----------YPLLLISAGSGVTPMLAMVRTALAlgklkgvslplaHIHF 425
gi 87122003  451 LVTNWLHDHAeLGLELVLTGIqGEMTAITldadtqtplvpEQLLLLSAGIGITPNLAMVRGIGAfslq-----dktKVTM 525
gi 149196883  72 LGSTWMHTQVkVGDELLCDEPeGKFTLQQa---------aLTSVFVAGGIGVTPVLSMLKYALSikd-------trKLLF 135
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 17548284  172 VHSARTPDDIIFARELELIasn-htNFRTSFVVerlgart-nwpgitGFLSLPLLKLIAPDFmeREIFTCGPApYMKAVR 249
gi 37676069  464 YHQCRSVEDIPCKEELDALksr-ypGLTVKISLtqapid---wfglkGRLSLSHLKQIKAVEs-RQVFVCGPEgFMQKAK 538
gi 89071919  406 YHYCKTQADIPFADELAEIqrh-hpEISIHICLtqdndt---shayhGRICAEHFANIDVKDn-CHVYVCGSSgFNQIAQ 480
gi 62423544  183 IHNAATVDDIIFRPELEQLae--vpGVSVVTMCsrdcaae-vwagrrGRITSQSFAEVVPELrdRETFVCGPGgYMAAVR 259
gi 117618335 477 MHLCRSEADIPAAGELHALaq---qGMTLTLILsqpdth---wqglkGRLGDDHLKQVKQLAe-REVFICGPHgFMADAA 549
gi 45441063  167 IFNVRTPADVIFANEWHTLlqrypqQLKLTLMAesaat----agfieGRINARIMQQVAPDIahRRVMTCGPApYMAWVE 242
gi 32033385  147 IHSVHSPEDVIFKSEWQELkak-ypQLNLVMNAsvgat----egfasGRISAEIIKNAVPNVsdYTVMTCGPEaYMAALK 221
gi 119775766 426 IHQCRSEDDIPCHKSLEDFva---aGMTLELNLttpgie---wkgnqGRVSASQLAAVPLLAe-RDAFICGPSgFMQTVE 498
gi 87122003  526 IHVERYQRHLLSHHELVRRshk-ypDFSYTNIIts----------eqGRLTKAQLEALVPNPkeQQAYVCGPAlFMAQMT 594
gi 149196883 136 FYASRHLEDLVFHQELLDLaa---aHPNLIYVPiisgdqdpewqgqrGRVNKELLEDTGVIFkrAEFYTCGPDaMMKNLE 212
                        250
                 ....*....|....*...
Feature 1                       # #
gi 17548284  250 GLLEEAGFdpARYHEESF 267
gi 37676069  539 NLLLKKGLpeSHYHQEAF 556
gi 89071919  481 ELFSNQGLptDRFHQEQF 498
gi 62423544  260 LMLDELGVlgSRVHEESF 277
gi 117618335 550 ARLVALGVpaERIRQESF 567
gi 45441063  243 QYCQQQQVpaDHFQQEQF 260
gi 32033385  222 NIVLELGVseDRFFTEAF 239
gi 119775766 499 GMLTQGGMvtSQIHCESF 516
gi 87122003  595 EYLASIGVpvANIHTEAF 612
gi 149196883 213 ELALANKVskSNFHKELF 230

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap