Conserved Protein Domain Family
FNR_iron_sulfur_binding_2

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cd06216: FNR_iron_sulfur_binding_2 
Iron-sulfur binding ferredoxin reductase (FNR) proteins combine the FAD and NAD(P) binding regions of FNR with an iron-sulfur binding cluster domain. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99812
View PSSM: cd06216
Aligned: 32 rows
Threshold Bit Score: 246.753
Threshold Setting Gi: 93004974
Created: 23-Jan-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:contacts primarily in the N-terminal region
  • Comment:based on Acinetobacter sp. Benzoate dioxygenase reductase (1KRH) and Methylococcus capsulatus Methane monooxygenase (1TVC)

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                              #             ####        
gi 145594608  39 LPDDYLDLIAPLRAGtdLRGRIVEVRPETPDaATVVIQPGRD----WQGHRPGQYVRLGVDVNGVRqWRAYSVTSapgd- 113
gi 94500836   30 FFEPLIQWIKPNWRAdrCYTRVVSVRYESSDmYTLQLKPLQS----FSHEFAGQYVELIIQKDGAWvSRYFTISSspeff 105
gi 88857834   24 YLEPLVQQFKPAWRAgfYRAKVIKIITLPANcISLELRPERS----WPSHQAGQHVLLSCQFNGRLmTRTFSISSsqgqa 99
gi 146305703  32 DVDLCLRLLHPALRLnrVFAQVEARRWVADDmLAIELRCNGN----ARDWRAGQHVQLYLEQDGVRhGRSYSLTRvtd-- 105
gi 119876463  33 LFDFWAGQLNPLWTLrePVARLVRREPAGEGaATLVLRTNRH----WAGMRAGQHVTLGVEIEGRLwQRSYSPTAlg--- 105
gi 21233302   32 LFDFWATRINPLWTLerPMARLVARTPASRDaVTLLFQPNGH----WQGLQAGQHVSIGVEIDGRRlLRSYSPTVla--- 104
gi 149927889  59 FVTFWLEQAGSTHSTdrCLAEVVRIDRETADaVSLWLRPNAL----FKGFEAGQHVNLSITIDDVVhTRSYSFSCapt-- 132
gi 9951164    29 DVDGLLRHAHPLASLnrHYARVEARYWVADDmLALALRTAPG----WPPVRPGQHIQLLAERDGVRvGRSYSLTRvv--- 101
gi 119469360  22 YIEPIMQVFKPAWRAgqFRAQLMSSESLNGEfLSLQLKPSKQ----WPAHTAGQHVSLTIEMNGRLlTRVFTVASsvkyf 97
gi 149907651  22 YFSPFSTKPVAVLMGdmFAAKVVAIKPENNDmYSLTLAVSGKvnaqSFSFQPGQYVELQVKKNGSYvKRPFTISSplaql 101
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ### #   ###                                              #  #           
gi 145594608 114 -rhdPITITVKAIpdGLVSNHLVRHAqPGTIVQLDQAQGDFVLPAt--------pPARVLLVTAGSGITPVMGMLRSGal 184
gi 94500836  106 retgTIELSIAIQanGRITPWLMKNLnKGDMVNLGQPMGDFLPLNipr----dksVSRKVFIAGGSGITPFRSSLQSLmq 181
gi 88857834  100 ergnHIRLTIKTAknGAFTSQLANELaVGDFVNLSEPMGEFLLPScep-----nsTDELFFIAAGSGITPFMAMLHTLae 174
gi 146305703 106 --dgRIELAIKRQpgGRLSNRLLDHLaVGEVIELGQAFGDFAWPQe---------QGAVLLLAAGSGLTPLLGLLRDAla 174
gi 119876463 106 --rrELAITVKAIdgGRVSQHLVNHAqPGELFRLDAAFGEFQLPA----------AAPVLLLAAGSGITPMRSLLREAcq 173
gi 21233302  105 --dgRLAITVKAItgGLVSTYLAGEApLGAVVSLDPAFGDMLLPTt---------PAPLLLLAAGSGITPMRALLQAAaa 173
gi 149927889 133 -dsgLVRLTIKQTptGLVSRYVVNQLeVGAVVELGDVFGDMTLAHtqsn--lsaqRPTMLLLAAGSGITPMISLIEELea 209
gi 9951164   102 --geRLEIAVKRHpdGLLSPWLCEHLaVGQRVELQPAQGDLRWPMq---------AEAVCLLAAGSGLTPLLGLLREAle 170
gi 119469360  98 netgFIRLLIKTSeqGRFTGLLNNKAkAGTWCNISAATGEFVFKNt---------HTPATLIAGGSGITPMIAMLDDYla 168
gi 149907651 102 qqdkTITLTIKKQskGRITPWLADAVeKGDLFGISPAKGDFLLSAallhsankqeNKDVIFIAGGSGITPFHSLLMSHva 181
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 145594608 185 t----gsDVTLVHSAPTAaDVIFGGALRELa-gAGALRLVERHtr--------ddGQLSLADLAALVPDHle-------r 244
gi 94500836  182 sderdtqEIELFYYARSEeHFLFREELQRCa--YLLPKAHVHFmds------ekiGFFDYTHLQVENKHQtd-------t 246
gi 88857834  175 rdhirtqNIYLCYFAKQG-EHLFIDELTELkrfIPRLNVMFLVrk---------qGDSLASALKNVMPTSq--------- 235
gi 146305703 175 rg--faaPVTLLHQVRRQgQQAFAEELQALaarHANLQVRWAFsge-------agGRLTAAQLAALPGEH---------- 235
gi 119876463 174 rp--laaPVDLFYWERTAaHLQFRDELQALaaaHPNLRVHLLTtr---------eGEAPAARIDTHDLLVpgddtplaqr 242
gi 21233302  174 ag--mpmDVDLLYWVRQRdEACFLDEFEALaaaHPRLRAQLLTtr---------eGAAPAPRIDSFPLEQvtal---qdr 239
gi 149927889 210 kg--wpaDVTLMSWARNPeDVLFDTSLKAKa--NEHFKYVRLLesganlaegdlaGRPSAEQFAAVAGALet-------t 278
gi 9951164   171 ng--yrgPVLWLHYVRHPgQRAWLGELEALaarHPNLELRWSLtrgara-dgcleGHFQPRHLDGWAADLar-------s 240
gi 119469360 169 q---ttkAINLVYYAKSG-EHQCVDELRELatqFSNFSFLLLT------------REQSSDITSNIKHWEnp-------- 224
gi 149907651 182 nn--ptiNFTLLYFVRAN-QHVLSDTLVALeleHANFTLKLLHse--------rdGYCSKAHINAITSELnd-------c 243
                        250       260       270
                 ....*....|....*....|....*....|...
Feature 1                                      # #
gi 145594608 245 eTWACGPAGLLDALTAHWTAVGrg-eRLHTEQF 276
gi 94500836  247 aFFVCGPPAMIQHVRSTLNTHGvkkeNIYYEFF 279
gi 88857834  236 -VLICGPQQFKQDVDAVLNHFGhlslNRQAEFF 267
gi 146305703 236 -LLLCGPRGFVEQACGWWRDAGrg-gSLQAESF 266
gi 119876463 243 hVLACGPDGFVAAARERLAHQVa---GFQAEAF 272
gi 21233302  240 hVMACGPGGFVQAARARLQDRVa---RFQAEAF 269
gi 149927889 279 dVYACGPDGFMNALRGILGDTPk---SFHAESF 308
gi 9951164   241 qALACGPAAFVEAVGEQLGARFa---GLQTESF 270
gi 119469360 225 dIYCCGPLGFMQRVQAFAQKHDl---NYYQEAF 254
gi 149907651 244 aVYICGPNSMMDSTQTLLLDMGlaqdAIHLEQF 276

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