Conserved Protein Domain Family
FNR_iron_sulfur_binding_3

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cd06217: FNR_iron_sulfur_binding_3 
Iron-sulfur binding ferredoxin reductase (FNR) proteins combine the FAD and NAD(P) binding regions of FNR with an iron-sulfur binding cluster domain. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap between the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99813
View PSSM: cd06217
Aligned: 22 rows
Threshold Bit Score: 244.098
Threshold Setting Gi: 170781913
Created: 28-Jan-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:contacts primarily in N-terminal region
  • Comment:based on Acinetobacter sp. Benzoate dioxygenase reductase (1KRH) and Methylococcus capsulatus Methane monooxygenase (1TVC)

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              #                ####             ### #   
gi 158317327  16 WRVARLAEVRDETPTARTLVLDVPGW-----PGHLSGQRVDVRLtaaDGYSt--rRSYSLAAPGAgd-rVELTVQRVpGG 87
gi 27353080  293 KGKLRICAIYQETPNVKTFRLQAPDGga-ipFAFLPGQFLTYAIe-iDGQAv--rRSYTIASSAAqtayVETTIKREdGG 368
gi 88794160  299 SGGMVVTAIFNETHDVKTLRLASPDGkt-ipFDFEPGQFVTFTLn-iDGFEkpvkRSYTIASSPTeqyyFEVTIKREeFG 376
gi 146283682 389 VGKLRVARVFQETPKVKTFRLAPVEGvgalpFEFEPGQFLTLSVh-sGGNQv--kRSYSIASSPCchgwCDLTVKHEsGG 465
gi 149176110  16 LISLPVTKVIQEADDVCTFRLDNSQGl---iPPHQPGMFVKVCLd-iNGVEv--wRSFSISSSPHqrerIDLTIKRNhAG 89
gi 54025061   44 RLQLVVAEIVDETTTTKTFRMQGLDQav--lPPFLAGQYVNVFV---AGTS----RPYAISSSPTqrghYDLTVRRVpGG 114
gi 159036699  52 KLELTVTELIDETSTTKTLRLRRAAGgs--lPPFRAGQYLSLDVqvgDIHTn---RAFSISSSPTrrdhYDLTVRRLpGG 126
gi 167775096  54 RIHLRVESIRIDTPSTKTLVMVPVDGnr--lPPFQAGQYINLFVt-lAGVLt--aRPYSISSSPKnlqsYELTIKRAeGG 128
gi 159036715  45 QLDLVVDEVITETATTKTFRLRRATGel--lPPFQAGQYISLRLq-iEGVRt--sRAFSVSSSPTerrhYDLTVRRVpGG 119
gi 161389352  48 RLNLTVRRIIRETGTAVTLRLARSDGem--lPPFQAGQYVNLFVt-vDGTHt--aRPFAISSPPQirthYDITVREVpGG 122
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ###                                        #  #                                 
gi 158317327  88 EVSPYLTEIFsVGDPVEIRGPIgGWFVWRpa-dpEPVLLVAGGSGIVPLMAMVRARRSAasrVPFRLICSVRSpaDLYYA 166
gi 27353080  369 LLSDYMHGHLkEGDLVEVAGPS-GAFTFTgv-eaDSVVLIGGGVGITPLMAAIRYLSDIawpGQIYLVYGAQTteQFIFR 446
gi 88794160  377 VVSRYMHDAVeVGNTLSIKAPG-GKFYFNgh-gaNSVVLISGGVGITPMMSAVRYLTTTcwdGDIYFLFCTRTsnDFIFE 454
gi 146283682 466 IVSGYLHEQVkEGDLLDASGPY-GRFTFRgv-esDSVVFLGGGVGITPLMSSIRYLTDQswnGRIDLVYACKDleSVIFR 543
gi 149176110  90 QVGNYFFDHIhVGSHVFLKGPL-GQFHFDpaqhvEPVILLCAGIGITPMMSIVRYLHATnvnRICYLFYGARThrDIIFD 168
gi 54025061  115 RISNLLIDTVvVGQVLTTTGPM-GTFHHNplfhgNDVVFLAGGSGVAPAMSMLREIADLgldRSLHLIYGSRHasDVIFH 193
gi 159036699 127 LVSNYLLDEVsIGDRFTSGGPM-GTFTHDplfhgDDLVFLAGGSGVAPAMSMIREIVELglpRRMTLLYGSRRsdDIIFQ 205
gi 167775096 129 FVSPYLLDDVkIGQKFESTGPM-GSFHHNplfhgFDIVFLAGGSGIAPAMSMLKAFLAAenpFRFHIIYSNSYenDVIFL 207
gi 159036715 120 RVSNHLLDAVaPGDRLVSSGPI-GTFQHNplfhgEDLVFLAGGSGVAPAMSMIREVVDRglpRTLHLVYGSRRadDIIFR 198
gi 161389352 123 FVSPYLVRGLtEGQLLQSSGPM-GTFYHNplfhgDDLVFLAGGSGVAPAMSMIHDFLSSarpPRFHLIYGSRNtgDVIFR 201
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 158317327 167 DELREAaradpGLDVTRCYTRvvPDHWTSPPRRLRpadlaeagwppdltpTCYVCGPTGFVEAVADALVEIGHepERIRT 246
gi 27353080  447 DELEYLqrrmsNLHVAATMVRaaGTSWMGSEGQITaefltqa-vpdlarrRVHLCGPPGMMDALRKTLIGLGVprEQIKT 525
gi 88794160  455 QELKYLqarhpRLKVLVSMTQaeGTSWMGPQGRFSsaminef-vpdiaskTAHICGPPAMMDATKKMLAELGMpdTHIKT 533
gi 146283682 544 DELNQLarrhpNLHVSIVLSDesSAAWTGPRGFITaellgqi--pqirsrRIHLCGPSVMMDAVRNELGKLGIdlASVHS 621
gi 149176110 169 QETRDLitempDFHYFLTLSQp-VPHWLGYCGQLNfdfmisk-ipqvelsRFFLCGPRNFNQDFTLRLLEMGVphELIHS 246
gi 54025061  194 AELDALaaaqpGIRVDHVIAEp-DADWAGATGFLTadtiatl-ageldgrMVYVCGPQALYPYALDQLTALGHprKRIRF 271
gi 159036699 206 DELEAIerqhpNIVVHHILAQa-DPGWTGAVKPLNaplivkl-aaplagrMTYVCGPAYKYPYLVGELESLGLpgRRIRM 283
gi 167775096 208 DELRNLsllhkNFILTEFISRevSPEFKGFKGRLDivtlqtl-lsdvsskMFYVCGPTPFNEHCANLLADLGVksGRILI 286
gi 159036715 199 AELDQVardcpEITVDHVISEp-DADWSGPTGFLSaavverl-agplrgrMTYVCGPPAMYAYQLRQLDRVGHprRRVRL 276
gi 161389352 202 EQLHQLadrheSLTIDEVISEp-DADYSGHSGFLNadliakl-vgplegkTFYLCGPNAMCDFCQPELTKLGVseRKVHV 279

                 ...
Feature 1        # #
gi 158317327 247 ERF 249
gi 27353080  526 EAF 528
gi 88794160  534 EAF 536
gi 146283682 622 ELF 624
gi 149176110 247 EQF 249
gi 54025061  272 EAN 274
gi 159036699 284 EAN 286
gi 167775096 287 EGN 289
gi 159036715 277 EAN 279
gi 161389352 280 EAN 282

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