Conserved Protein Domain Family
DHOD_e_trans_like1

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cd06219: DHOD_e_trans_like1 
FAD/NAD binding domain in the electron transfer subunit of dihydroorotate dehydrogenase-like proteins. Dihydroorotate dehydrogenases (DHODs) catalyze the only redox reaction in pyrimidine de novo biosynthesis. They catalyze the oxidation of (S)-dihydroorotate to orotate coupled with the reduction of NAD+. In L. lactis, DHOD B (encoded by pyrDa) is co-expressed with pyrK and both gene products are required for full activity, as well as NAD binding. NAD(P) binding domain of ferredoxin reductase-like proteins catalyze electron transfer between an NAD(P)-binding domain of the alpha/beta class and a discrete (usually N-terminal) domain which vary in orientation with respect to the NAD(P) binding domain. The N-terminal domain may contain a flavin prosthetic group, as in flavoenzymes, or use flavin as a substrate. Ferredoxin is reduced in the final stage of photosystem I. The flavoprotein Ferredoxin-NADP+ reductase transfers electrons from reduced ferredoxin to FAD, forming FADH2 via a semiquinone intermediate, and then transfers a hydride ion to convert NADP+ to NADPH.
Statistics
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PSSM-Id: 99815
View PSSM: cd06219
Aligned: 39 rows
Threshold Bit Score: 235.163
Threshold Setting Gi: 156719283
Created: 6-May-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:FAD binding pocket [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                 # ####             ###   ###           
gi 9297059     7 NEIVKKVKIaedVFDFWIHSpSVSKEARPGQFVVIRLHEKGERIPLTVADtkpeeGLFRMVVKVVGKTTHELSLKk-eGD 85
gi 73747992    2 YTIIERQDLlpkVHLLKIQApKVAAAAAAGQFVILRVDTEGERFPLTIADwdaqaGTVTVIYMEIGVSTRKLAKLg-kGD 80
gi 170287272   2 YKIISKKEIgpkVRTYEICApEIARNAKAGQFVIFRIDDKGERVPLTVAGtdpqkGLVRIIFQEVGKSTMQLASLc-eGD 80
gi 39937780  651 QTIVGKVPLaggIVEFTVRApMVARSAKAGQFVRVLPWEKGELIPLTLADwdaekGTIDLVVQGMGTSSLEINRMa-iGD 729
gi 89901977  654 QTILQKIHLadgIVEFIVHSpLVAKAAQAGQFVRVLPWEKGELIPLTLADwdakaGTICLVVQAMGASSIAINAMl-vGD 732
gi 149189297 619 AKIVKRRQLtdeIVEFTITApLIARSAKPGQFVRVLADEKGELIPLTLADwdaekGTIELVIQGLGSSSKLINRMq-eGD 697
gi 116754224   2 YRICEKEVLapnIIHMCFEApRIAGAARPGQFLIVRVDERGERLPLSLAGwdpekGTVDVVFYVLGTSTMKLASLk-eGD 80
gi 3258313    11 YKILEKKEIamrNTWYKIYApHVAKKVQPGQFVIVRAFPNGERIPLTPIMwdreeGWIVLVAFTKGKTTMKMAMElkeGD 90
gi 116749581   2 FPIVLHEELspgVHRMSVKApEVARKCGPGQFVIVIIDEKGERIPFTVSGwdtaaKTVDFVYVEVGKSTRQLAGLk-pGD 80
gi 14590735    6 YKITDKRDLspiDFFVEVEApHIAKAWKPGQFVIFILHERGERVPMSVYKae--nGRIGMFIRKLGKTSLQLYYEfnvGD 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                      #                                                 
gi 9297059    86 TILDVVGPLGNPSEIen---yGNVLLVGGGVGIA-TLYPIAKALKeAGNNITTVLGARTkDYLIMVDEFke--------- 152
gi 73747992   81 AILDFAGPLGKPTEIen---fGTVVCVSGGFAMA-TIVPIARAMKeAGNRVISIVGARSkDLFFWHDRLtky-------- 148
gi 170287272  81 CIKDFAGPLGRPTEIkk---yGTVVAVAGGVGTA-EVLPVIKELKqAGNRIITVVGARCkDLIILEDELkre-------- 148
gi 39937780  730 AFSGIAGPLGRASELhryegnQTVVFCAGGVGLP-PVYPIMREHLrLGNHVTLISGFRAkEFLFWTGDDervgklkkefg 808
gi 89901977  733 AFTGIAGPLGLPSELhrfegqQTVVFTAGGLGLP-PVYPIMRAHLrLGNHVTLIAGFRNaDLLFWVKAGervsqlqaefp 811
gi 149189297 698 VFEAIAGPLGQASKVvvhqdnHRVYFTAGGVGLP-AVHPIMRAHLeIGNKVTLISGFRGaPQAFWTEQGdkvetlkalyp 776
gi 116754224  81 CVLNLAGPLGEPTEIsh---fGDVICACGCFGIG-PAIPLIKSLKdAGNRVVTVVEGRSrSFIFWEDRLrsl-------- 148
gi 3258313    91 EILNVAGPLGNPAPMek---fGKILAIGAYVGIA-EVYPIAKAWQeIGNDVTTLHVTFE-PMVILKDYFeka-------- 157
gi 116749581  81 NLAHLVGPLGRAAEIdr---fGHVVAVASGYGIA-AIVPVLDALRaKGNRVTTILQAPDvERIFGRSALers-------- 148
gi 14590735   84 EFYSMVGPLGKPIKVky---yGNVVFASDAVCGQaENYATLKAMKeVGNYTISIQTFENaENVYPEEFLakp-------- 152
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 9297059   153 -isDVLLVTDDGSa----------GMKGVVTdamdklf------rerkfdiCWAVGPTIMMKFCTLKarefgvpIWVSLN 215
gi 73747992  149 -sdEMIVCTDDGSa----------GIKGLVTeplrdlls-----sdtkldrVIVIGPSVMMKFCSKTtqpfgvkTIVSLN 212
gi 170287272 149 -sdLLLFATNDGTl----------GIKGFVTavlkdvi------srekidiAYAIGPVPMMKAVAELtmlkrikTLVSLS 211
gi 39937780  809 dqlSLIYTTNDGSy----------GVKGFVTgpleemmkanqegkgrsiaeVIAIGPPLMMRAVSDLtkpygvkTVASLN 878
gi 89901977  812 dllDVIYTSNDGSf----------CQKGFVTtpleamlkdgqtsqgrkiaeVVTIGPPLMMRAVSDLtkpygvkTVASLN 881
gi 149189297 777 dllEVIYTSNDGSf----------GVEGFVTnplkdrldehqagqgepiaeVITIGPPLMMRAVSDLtleykveCVASLN 846
gi 116754224 149 -sdKLHVVLGDGSc----------SEPGWANefisryls-----egnrvdrIFVHGCPFMMMVVSEAsrpfgvkTVVSLT 212
gi 3258313   158 -vsRHIVEPVKIDqeksfqentknVTKALTAkvkelld-------nenwdlVFMVGPPGDQKAVFEVvkkygipMKVDLH 229
gi 116749581 149 -sdRLILAAGENGp----------DMTASATfplrrllad---hrgspvdrVIVMGSICLMRIISEMtrpygvrTMVHLT 214
gi 14590735  153 vadEHYLTTEDGSv----------GIKGHYLdivkeli------ekdkvdiIFAGGKLGSLAKLAELtrpygipTYATVR 216
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|.
Feature 1        ##                                       
gi 9297059   216 PIMVDGTGMCGACRVTv----sGQIKFACVDGPEFRGEEVD 252
gi 73747992  213 PIMIDGTGMCGCCRVSl----dGQTRFACVDGPEFDGHKVD 249
gi 170287272 212 PIMLDGTGMCGACRVSi----gGKMKFVCVDGPEFDAHYVN 248
gi 39937780  879 SIMVDATGMCGACMVPvtidgkMVRKHACIDGPEIDAHIID 919
gi 89901977  882 SIMVDATGMCGACMVPvtidgkMVRKHACVDGPELDSHIID 922
gi 149189297 847 SIMVDATGMCGACMVPvmedgkLIRKHACIDGPEINSHVID 887
gi 116754224 213 PLMVDGTGMCGACRVEv----gGETRFACVDGPDFDGHLVN 249
gi 3258313   230 PIMVDGTGMCGACRVTv----gGEVKFACVDGPEFDAYLVD 266
gi 116749581 215 PLMVDGTGMCGACRLSv----dGNNRFACVHGPEFDGHKVT 251
gi 14590735  217 QIMVDGTGMCGSCRILy----dGEVKFACRDGPVFDAHKVD 253

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