Conserved Protein Domain Family
PBP1_LacI_like_13

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cd06299: PBP1_LacI_like_13 
Click on image for an interactive view with Cn3D
Ligand-binding domain of DNA-binding regulatory protein from Corynebacterium glutamicum which has a unique ability to produce significant amounts of L-glutamate directly from cheap sugar and ammonia
This group includes the ligand-binding domain of DNA-binding regulatory protein from Corynebacterium glutamicum which has a unique ability to produce significant amounts of L-glutamate directly from cheap sugar and ammonia. This regulatory protein is a member of the LacI-GalR family of bacterial transcription repressors. The LacI-GalR family repressors are composed of two functional domains: an N-terminal HTH (helix-turn-helix) domain, which is responsible for the DNA-binding specificity, and a C-terminal ligand-binding domain, which is homologous to the sugar-binding domain of ABC-type transport systems that contain the type I periplasmic binding protein-like fold. As also observed in the periplasmic binding proteins, the C-terminal domain of the bacterial transcription repressor undergoes a conformational change upon ligand binding which in turn changes the DNA binding affinity of the repressor.
Statistics
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PSSM-Id: 107294
View PSSM: cd06299
Aligned: 13 rows
Threshold Bit Score: 333.149
Threshold Setting Gi: 16802079
Created: 20-Jun-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
putative liganddimerization
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:putative ligand binding site [chemical binding site]
Evidence:
  • Comment:based on structural similarity to other members of the LacI family.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    ##  #                                                     #         
3BIL_A        68 TIGVIVPSLiNHYFAAMVTEIQSTAskagLATIITNsneda---------ttmsgSLEFLTSHGVDGIICVpneecanq- 137
gi 171741557  86 TVGLLVPDIrNGYFADLAYAIQDTLskagYCTFIGTssenv---------rqqdvFITNMLSQHIDGAIIVpqgemsea- 155
gi 38199522   84 TIGIVLPNIrNPYFNQLASAIQEAAslkgYCCMMANsaesp---------elinsALDILNSQQVDGIIVVphiqsaqq- 153
gi 23466312   63 TVGLLVPDLrNPYFADLVSSMEDELyargYSTLIGTsaetv---------erqdaFIDNLLGQRIDGAIVVpqgvnspg- 132
gi 25028041   70 TIGIVVPSLiNPYFATMVTAVQNAAegagLATIITNtdehp---------etmaaSLTFLASHGVDGIICVpdeacaeq- 139
gi 19552472   72 TIGVIIPNIeNPYFSSLAASIQKAAreagVSTILSNseenp---------ellgqTLAIMDDQRLDGIIVVphiqseeq- 141
gi 19552582   67 TIGVIVPSLiNHYFAAMVTEIQSTAskagLATIITNsneda---------ttmsgSLEFLTSHGVDGIICVpneecanq- 136
gi 25027905   88 TIGLLVPNIeNPYFASLAAAVQRRArragVSTILTNteenp---------dlvnqALDVMDDQRLDGIIVVphfqsadk- 157
gi 50841508   64 VIGLVVPDIrNPYFAELASVVENAClaygWATLLCNadesp---------dqfnrYVNTLRRQRVDGAIVTptgsggqa- 133
gi 50839110   64 VIGLVVPDIrNPYFAELASVVENAClaygWATLLCNadesp---------dqfnrYVNTLRRQRVDGAIVTptgsggqa- 133
gi 149276660  66 TIGVIIPNLaEPFFSSALCGVEDFAepfnYTVIIGQsldtl---------drekrILENMKDHRVDGILVSlskdtntyd 136
gi 16412451   63 NIGVILPDIsNPYFISLITQIQKFAldymFSTILFNtmlaepnnknskhplteedYLKIILEKQVDGLLILggeidkevv 142
gi 16802079   63 NIGVILPDIsNPYFISLITQIQKFAldymFSTILFNtmlaepdnknnqhplteedYLKIILEKQVDGLLILggeidkeiv 142
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                            #             #                                    #        
3BIL_A       138 ------ledlqkqGMPVVLVDrelpgdstipTATSNPQPGIAAAVELLAhnnalPIGYLSGpmd-tstGRERLEDFKAAc 210
gi 171741557 156 ------lqrllrrQLPVVFVDrradeldmvpLVDSDPFPGMESALRDIQeqghsKVGYVSGpildsptLRERELAFRDCg 229
gi 38199522  154 ------itaiteqGTPIVAADrtvth-savpSVTSDPLPAMKEALRMLSlnpgaSIGYLAGpqd-tstGRERLEAITSIk 225
gi 23466312  133 ------mqsliarELPLVFVDrlvsgvnsvpFVVSDPYPGVCEAVAELVrlghrHIGFVSHsslgssnINEREAAFRSAv 206
gi 25028041  140 ------lnnlhaqGMPLVLIDreltg-spipTVISDPQPGMDAAVSLLVehhalPIGYLSGpls-tstGRARLEAFQQAc 211
gi 19552472  142 ------vtdlvnrGVPVVLADrsfvn-ssipSVTSDPVPGMTEAVDLLLaa-dvQLGYLAGpqd-tstGQLRLNTFERLc 212
gi 19552582  137 ------ledlqkqGMPVVLVDrelpgdstipTATSNPQPGIAAAVELLAhnnalPIGYLSGpmd-tstGRERLEDFKAAc 209
gi 25027905  158 ------vaelharGVPIVLADrtlkd-tgipSVTSDPEPGMTAAVDLLTat-dvSLGYLSGpqd-tstGRERLGLFEQLc 228
gi 50841508  134 ------vkdlvddGVQVVCVDreitg-splpAVTSDPWNGMRQAVHHLVdhghrRIGFITGpqq-tstGLERYQAYRQLl 205
gi 50839110  134 ------vkdlvddGVQVVCVDreitg-splpAVTSDPWNGMRQAVHHLVdhghrRIGFITGpqq-tstGLERYQAYRQLl 205
gi 149276660 137 -----hfeslkkyNIPVVFFDrvpdk-kdihFVAGNLHSGMLQAITKLVnmghkKIGLINGpak-laaTLQRLELYLKCl 209
gi 16412451  143 skdyinalnqlnrEIPVVVIGskipe-lnclFIERNLKKGITTLVSHLTaldhkNIGFIGGeag-vkiTSYRLESFKAAm 220
gi 16802079  143 pkeyitalnqlnnAIPVVVIGskipe-lnclFIERNLKKGVTTLVSHLTalghkNIGFIGGeag-vkiTTYRLESFKEAm 220
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         #                                                      #       
3BIL_A       211 ansk---igeqLVFLGGYeqSVGFEGATKLLDqg---akTLFAgDSMMTIGVIEACHKAglvigkdVSVIGFDTHplfa- 283
gi 171741557 230 trlf--geenvFVESTSFdqSSCVSVIRRMLSsg---vtSMVFgYSPDTVRAIAMMNGVggvlesgMSFISFDDIelfr- 303
gi 38199522  226 selg---ipspDVYFGGYssEAGFAGTHELLDrg---vnSILTgDMMMTVGAIQAIYARglrigkdVALIGYDNTpafl- 298
gi 23466312  207 aqvtqlgegtaAVVDCDStyVSREAGLNELVRag---vtAIICaYSPDMITMIGLLHDRgidigseMSVISFDDIavfr- 282
gi 25028041  212 rnhd---lepqLVFRGGFeqEQGYQGATHLLNhg---arALVAgDTMMTIGVLQACHRAglsigedISVIGFDNQpifa- 284
gi 19552472  213 vdrg---ivgaSVYYGGYrqESGYDGIKVLIKqg---anAIIAgDSMMTIGALLALHEMnlkigedVQLIGFDNNpifr- 285
gi 19552582  210 ansk---igeqLVFLGGYeqSVGFEGATKLLDqg---akTLFAgDSMMTIGVIEACHKAglvigkdVSVIGFDTHplfa- 282
gi 25027905  229 vdrg---itgvSVYYGGYrqESGYEGVRVLLEqg---anAIIAgDSMMTIGALMAIHEMdlvigedLQLIGFDDNpvfr- 301
gi 50841508  206 aehgl-evdedLVVEGDFqeESGRMGARRLLDgg---atAIFAsDSLMSMGALRTCIDQgvrigdeIDLVGFDDLpvfs- 280
gi 50839110  206 aehgl-evdedLVVEGDFqeESGRMGARRLLDgg---atAIFAsDSLMSMGALRTCIDQgvrigdeIDLVGFDDLpvfs- 280
gi 149276660 210 rsndl-evnydLIVSTTLskTDNVTAMQKLMAleq-pptAVIVfNDYVLLDCIATVKAAnlvvnkdISFISFANLpiwey 287
gi 16412451  221 asynh-svnedWIVLSDYytADGYAAMTKLLEnnttlptALVAiNDNVAIGAIRAINDAklscpedIAIVSCDQFmngd- 298
gi 16802079  221 tsyqh-pvnddWVVLSDYytADGYAAMTHLLEnsttlptALVAiNDNVAIGAIRAINDAklscpedIAIVSCDQFmngd- 298
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|..
Feature 1                  #                                         
3BIL_A       284 lqpHPLTVIDQNvEQLAQRAVSIltelia-gtvpsvTKTTI--PTALIHRES 332
gi 171741557 304 lttPQVSVISQQvQRLGCLGAETfldmv---qgghpRSRRV--ETIYIQRGS 350
gi 38199522  299 lqeQPITTIDQQvDDMGQRAFDMlyshmn--ngeraTSCVI--PTTLALRAS 346
gi 23466312  283 lltPQVAIISQQaEDMGRQGVDMlldmiinndcsrgESRYV--PSSFVLRDS 332
gi 25028041  285 lqpRPLTVIDQQvDVMARQAFTVlhglla-gtqpthNHLVT--PTILIQGDS 333
gi 19552472  286 lqnPPLSIIDQHvQEIGKRAFEIlqklin--gdtaqKSVVI--PTQLSINGS 333
gi 19552582  283 lqpHPLTVIDQNvEQLAQRAVSIltelia-gtvpsvTKTTI--PTALIHRES 331
gi 25027905  302 lqnPRLTVIDQQvEKMGEHAFDLlqqlia--gkggvESVRI--PTTLHIHGS 349
gi 50841508  281 ltnPALTVVAQDiDAMGKVAVDLlnram---sgeptSSARL--DTHLIVRAS 327
gi 50839110  281 ltnPALTVVAQDiDAMGKVAVDLlnram---sgeptSSARL--DTHLIVRAS 327
gi 149276660 288 mdtMPLGSIEQFaYQQASKATEFlfqlieggnqpqpAEVKF--LQCIIDSEM 337
gi 16412451  299 yqtPRLTSMDQHnEYLGKMALLQlisai----ngqvEPMIInhNPELIIRES 346
gi 16802079  299 yqtPRLTSMDQHnEYLGKMAILQlisai----ngqvEPMIInhNPELIIRES 346

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