Conserved Protein Domain Family
PBP1_NPR_GC_like

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cd06352: PBP1_NPR_GC_like 
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Ligand-binding domain of membrane guanylyl-cyclase receptors
Ligand-binding domain of membrane guanylyl-cyclase receptors. Membrane guanylyl cyclases (GC) have a single membrane-spanning region and are activated by endogenous and exogenous peptides. This family can be divided into three major subfamilies: the natriuretic peptide receptors (NPRs), sensory organ-specific membrane GCs, and the enterotoxin/guanylin receptors. The binding of peptide ligands to the receptor results in the activation of the cytosolic catalytic domain. Three types of NPRs have been cloned from mammalian tissues: NPR-A/GC-A, NPR-B/ GC-B, and NPR-C. In addition, two of the GCs, GC-D and GC-G, appear to be pseudogenes in humans. Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) are produced in the heart, and both bind to the NPR-A. NPR-C, also termed the clearance receptor, binds each of the natriuretic peptides and can alter circulating levels of these peptides. The ligand binding domain of the NPRs exhibits strong structural similarity to the type I periplasmic binding fold protein family.
Statistics
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PSSM-Id: 107347
View PSSM: cd06352
Aligned: 92 rows
Threshold Bit Score: 223.727
Threshold Setting Gi: 1850774
Created: 16-Jan-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:1YKI; Human NPR-C complexed with brain natriuretic peptide, contacts calculated at 3.5 A.
    View structure with Cn3D
  • Structure:1YK0; Human NPR-C complexed with atrial natriuretic peptide, contacts calculated at 3.5 A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1DP4_A          4 TVAVVLpltntsypwSWARVGPAVELALarvkarp----dllpgwtVRMVLGSsenaagvcsdtAAPLAAVDLKWe---h 76
1YK1_A         53 EVLVLLpqdd-sylfSLTRVRPAIEYALrsvegngtgrrllppgtrFQVAYEDsdc------gnRALFSLVDRVAaarga 125
gi 1706243     54 KIGVVGpwac-dslfSKALPEVAARLAIerinrdp----sfdlsysFEYVILNedc-----qtsRALSSFISHHQ----- 118
gi 1706245     71 TLGVLGpwdc-dpifAQALPSMATQLAVdrvnqda----slllgsqLDFKILPtgc-----dtpHALATFVAHRN----- 135
gi 1840403     54 KVGVLGpwnc-dpvyYRALPTVAARLAIsrinrdp----nldlgltMDFIILQepc-----etsKAHTTFIYYDK----- 118
gi 4521264     67 RVGVVGpwgc-dpifAKALPSTAAQLAIthinrdp----slssagaFDFAVLQepc-----etsRALETFVGFHT----- 131
gi 47209256    29 KVGVLGpwnc-dpvyHRAFPAAAAKLAGsrmnrdp----sldlgleMEFVTLQepc-----etsKALNAFIYYEG----- 93
gi 47210702    17 RVGVVGpwec-dplfAKALPHVAAQLAVnrinrdp----slsyaatFDYIVLQepc-----etsRALEAFLGFHT----- 81
gi 73988127    11 TLGVLGpwdc-dpifAQALPSVAAQLAVdqanqds----slvlgsrLVSVVLPtgc-----dtpHALATFLAHKN----- 75
gi 118085275   28 KIGLLGpwnc-dpffSKAFPHAAARLAVeriskdp----sidlgqrLDYVVLQeec-----etsRALVRFNDLGK----- 92
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       #   #                                                             
1DP4_A         77 SPAVFLGPGcvysaAPVGRFtahWRVPLLTAGApalgigvk-deyalttRTGPSHvKLGDFVTALHRrlgwehQALVLYA 155
1YK1_A        126 KPDLILGPVceyaaAPVARLashWDLPMLSAGAlaagfqhkdseyshltRVAPAYaKMGEMMLALFRhhh-wsRAALVYS 204
gi 1706243    119 MASGFIGPTnpgycEAASLLgnsWDKGIFSWACvnyeldnk-isyptfsRTLPSPiRVLVTVMKYFQwa---hAGVISSD 194
gi 1706245    136 TVAAFIGPVnpgycPAAALLaqgWGKSLFSWACgapeg------ggalvPTLPSMaDVLLSVMRHFGwa---rLAIVSSH 206
gi 1840403    119 SANAPVGPTnpgycIAASLLaksWDKALFSFSCisyeldrl-tayptfaRAAPFPaDVLFTVFKHYRwa---tSVVISSN 194
gi 4521264    132 KASGYIGPVnpgycDAASMLtkgWNKAVFPWACvgyelddv-rshptlaRSLPRPtWVLHNLLSYFRwa---hIAIISSY 207
gi 47209256    94 MVDAFVGPInpgycAAASLLsknWDKALFSITCvnyeleht-vgfptfaRTLPISvDVLFNVLKHFRwa---nVAVVSSN 169
gi 47210702    82 KAAGYIGPAnagycDAASMLskgWNKAVFPWGCvgyelddl-rshptfaHSMPRPtSVLLSVVRYFRwa---hIGIISSS 157
gi 73988127    76 TIAAFVGPVnpgycQAAALLaqgWGKTLFSWACgapeg------gselvPTLPSAaHVLLSIMRHFGwa---hVAIVSSH 146
gi 118085275   93 TSSAFIGPLnpgfcEEATYLvenWNKAIFSWMCvnykldst-thhpafaRTLPSPtRVLFTIMKYFQla---hVGIIASN 168
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  ##  #  ##           #                                                  
1DP4_A        156 DRlgddrpCFFIVEGLYMRvreRLNITVNHQEFvegd---pdhyPKLLRAVRRKgrVIYICssp-----daFRNLMLLAL 227
1YK1_A        205 DDkle-rnCYFTLEGVHEVf-qEEGLHTSIYSFdetk---dldlEDIVRNIQASerVVIMCass-----dtIRSIMLVAH 274
gi 1706243    195 EDi-----WVHTANRVASAl-rSHGLPVGVVLTtgqdsqsmrkaLQRIHQADRIr-IIIMCmhsaliggetQMHLLECAH 267
gi 1706245    207 QDi-----WVTTAQQLATAf-rAHGLPIGLITSlgpgekgatevCKQLHSVHGLk-IVVLCmhsallggleQTVLLRCAR 279
gi 1840403    195 EEi-----WIETAGRVASAl-rMKGLPVGFVAAmgmndtelestLKKIQSTGGVr-VIIMCidsvlvggeqQAVFLLKAK 267
gi 4521264    208 EDi-----WVETAIKVADSl-rSHGISVRLVSFmentphgirqtLSRVHKMQEIr-AVILCmhsaliggsaQKLLLEAAY 280
gi 47209256   170 EDi-----WRDTAGRVSTAl-rDRGLPVSLVTSmgmnktevessLRNIQNAGGVr-VIIMCmhsvlvggeqQATFLLKAK 242
gi 47210702   158 DDi-----WVETANKVADAl-rSHGLPVRLVSFmentphairhtLSKVRKMKELr-VVILCmhsvliggevQKLLLETAY 230
gi 73988127   147 QDi-----WVATARQVAMTl-rTHGLPVGLVTSlgpgeqgatevLEQLCSVDGLk-IVVLCmhsallggleQTALLSRAW 219
gi 118085275  169 QAv-----WMDTSNKLASAl-rSQGLPVGIVTSmgkgekgiedtWNKIKEVTGIk-IILLCmhsvliggeeQAALLKKAQ 241
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1DP4_A        228 NAgltgedYVFFHLdvfgqslksaqglvpqkpwergdgqdrsaRQAFQAAKIITYkepdnpEYLEFLKQLKLLAdkkfnf 307
1YK1_A        275 RHgmtsgdYAFFNIelfnsssygdgs------wkrgdkhdfeaKQAYSSLQTVTLlrtvkpEFEKFSMEVKSSVekqgln 348
gi 1706243    268 DLkmtdgtYVFVPYdallyslpykht------pyqvlrnnpklREAYDAVLTITVe----sQEKTFYQAFTEAAargeip 337
gi 1706245    280 EEgltdgrLVFLPYdtllfalpyrnr------sylvldddgplQEAYDAVLTISL------DTSPESHAFTATKmrggaa 347
gi 1840403    268 QLgltsgkYVFVPYdtlhysvpytnv------shfalknnsslKEAYDAVLTITMa----sEPLSFNEAFEAARksgeit 337
gi 4521264    281 DMkmtdgsLVFVPYdtllyslphrni------typalksnsklLRAYDAVLTVTId----sPHTSFYETYREVMergevp 350
gi 47209256   243 EMgltggqYVFVPYdtlhysvpynnv------shkplqsnsqlREAYDAVLTITVa----sEPLSFNEAFAAAKenqevt 312
gi 47210702   231 DMemidgsFVFVPYdtllyslpyrnv------tyaalksngklLRAYDAVLTVTId----sPRMSFYESYREAMekreva 300
gi 73988127   220 AQgladgrLVFLPYdtmlfalpyrnh------sylalgnsgplQEVYDAVLTISL------ESDPVDKAFEAAKasgeva 287
gi 118085275  242 EMgltdgrYVFIPYdtllyslpypnn------sfyvldndrklQEAYDAVLTITLd----sGNWTFYDAFREAKengeia 311
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1DP4_A        308 tv-edglkniIPASFHDGLLLYVQAVTETLAQGgtvtDGENITQrmWNRSFQGvtGYLkidrNGDRDTDFSLWDMdpe-t 385
1YK1_A        349 m---edyvnmFVEGFHDAILLYVLALHEVLRAGyskkDGGKIIQqtWNRTFEGiaGQVsidaNGDRYGDFSVIAMtdvea 425
gi 1706243    338 eklefdqvspLFGTIYNSIYFIAQAMNNAMKENgq-aGAASLVQhsRNMQFHGfnQLMrtdsNGNGISEYVILDTnlk-e 415
gi 1706245    348 anlgpeqvspLFGTIYDAVILLAHALNHSETHGtg-lSGAHLGNhiRALDVAGfsQRIridgKGRRLPQYVILDTnge-g 425
gi 1840403    338 lpvqpeqvnpLFGTIYNSIYLMARSIHNARKAGmt-lSGSNVAYytKNTNFDGfnQRFevdsRGQVKTSYVVLDSdsk-g 415
gi 4521264    351 ttlkpqqvspMFGTIYNSVLLMAHAIKGVRDSGaw-iSGGNLAHqtRNLNFQGfsHRIktssSGAVQLDYLILDTdgs-s 428
gi 47209256   313 ldvrpeqvhpLFGTIYNSIYILARSIHNTRKGGms-lSGSNLAYftKNIDFTGfnQNVkvdsQGQIRSSYVVLDTdst-g 390
gi 47210702   301 atlkpqevspLFGTIYNSIFFMAHAVHHLRQSGew-mSGGNLARrtRNLAIQGfsHWLrtnaSGSALVDYLILDTdgl-s 378
gi 73988127   288 ahlepeqvspLFGTIYDAVVLLAHALNRSESHGag-lSGARLGDhtGALNVAGfsQRIrtdeKGRRLAQYVILDTdgr-g 365
gi 118085275  312 seledtqvspLFGTIYDAIYFMALAMNSARRNGaw-vSGANITEhtRNISFHGfsHWVetdsCGKWLCNYVVLDTdgh-g 389
                         410
                  ....*....|....*....
Feature 1                            
1DP4_A        386 gaFRVVLNYNgtsqELMAV 404
1YK1_A        426 gtQEVIGDYFgkegRFEMR 444
gi 1706243    416 weLHSTYTVDmemeLLRFG 434
gi 1706245    426 sqLVPTHILDvstqQVQPL 444
gi 1840403    416 aeLYQAYVVDlrsgVLRFT 434
gi 4521264    429 seLLPTYRIDmeigMVRFL 447
gi 47209256   391 shLYRTYTVDlvsqRLRFA 409
gi 47210702   379 weLKPVYRIDmeagSVRFL 397
gi 73988127   366 sqLVPTHILDtgtwQVQPL 384
gi 118085275  390 sqLFPTHLLDmsseSVKPL 408

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