Conserved Protein Domain Family
PBP1_NHase

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cd06358: PBP1_NHase 
Type I periplasmic-binding protein of the nitrile hydratase (NHase) system that selectively converts nitriles to corresponding amides
This group includes the type I periplasmic-binding protein of the nitrile hydratase (NHase) system that selectively converts nitriles to corresponding amides, which are subsequently converted by amidases to yield free carboxylic acids and ammonia. NHases from bacteria and fungi have been purified and characterized. In Rhodococcus sp., the nitrile hydratase operon consists of six genes encoding NHase regulator 2, NHase regulator 1, amidase, NHase alpha subunit, NHase beta subunit, and NHase activator. The operon produces a constitutive hydratase that has a broad substrate spectrum: aliphatic and aromatic nitriles, mononitriles and dinitriles, hydroxynitriles and amino-nitriles, and a constitutive amidase of equally low substrate specificity. NHases are metalloenzymes containing either cobalt or iron, and therefore can be classified into two subgroups: ferric NHases and cobalt NHases.
Statistics
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PSSM-Id: 107353
View PSSM: cd06358
Aligned: 21 rows
Threshold Bit Score: 362.723
Threshold Setting Gi: 37718702
Created: 25-Jan-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
putative ligand
Feature 1:putative ligand binding site [chemical binding site]
Evidence:
  • Comment:based on sequence similarity to the Pseudomonas aeruginosa AmiC complexed with butyramide

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                   #     
gi 120401668    6 NIAMVIPRSGsagIYGPSCEACAELAIADANSaSGVLGREVRLVPVDGsrppAVVAAEVARLIECgAVDAVTGWHISPVR 85
gi 56696374    29 KVGVLLPESGpagLFGPSTRNSALLAAEAINAgGGINGEQIELVFADVgvppAEAAQAALRLWKGqGAQAFVGMHDSAVR 108
gi 148255810    5 RIGLLVPLSGsagLWAPSAEACGRLAVSELNQaAGIMRRPVELIVVNAgetgRSAAHAAREAVDElAVDGLIGMLPSYAR 84
gi 27381523    46 KIGVLVADSGpaaLFGPAQRAAAELGAATINAkGGILGRQVEIVFADAgappAEVAKSAVKLMLSdKVEMIFGSHDSAVR 125
gi 1487871     17 RLGLLTSKSGpgsLFATSIDNAAMLALEEINAeGGIHGSPVELIVGDDatdpQTGVMEARRLVQA-GCRTILVAATSATY 95
gi 119952742  112 RIGLLTSKTGpaaVFAVASEYMAELAIEEINAdGGLHRQSVELVVADDatnpVHAALEAHRLIAA-GCRAVIAGVTSASF 190
gi 146339662   29 KIGVPVGLSGansVVAPSVVQSAELAVEEINAkGGVLGRKLELEVADDasgaAGAQKAFDALIFQkKVNVLISMETSAAR 108
gi 117925926   19 RIGLMTPLTGlvkLYGPEISWAGQIACDEINEsGGVLGKSLELVIVDDgslpETAVPAAKELVDEhRCVAMIGNLLSNSR 98
gi 91781310    32 RIGVAVGLSGansVVAPAVVQSSQLAVEQINAsGGILGRQVKLEITDDasgaVGAQKAYDSLVFQkNVDAIISMETSAAR 111
gi 118043014   30 VVGVAVGLSGansVVAPSVVQAADLAVDQINAaGGVLGRKLRLEIVDDsssaAGAQKAYDVLVFQkKVDAIVSMETSAAR 109
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         #  ##                                          #                
gi 120401668   86 KAIARVTA--GRVPYVYGPLYEGgehtaglfLTGETPSRQLLPAMDWMKaeygidRWIVIGNDYifPRGTGVAAAKHAra 163
gi 56696374   109 EALIGRFN--GQVPYVYTPVYEGnscapglyVTGETPSQQLAPVIPYLAgkegvsKWYLIGHDYnwPRDTNALAKGYIae 186
gi 148255810   85 DPVVQATQ--GQVPFVYTPQFEGlatnadvmTTGETAGELMAPAIRWLSeakrarRFFLCGNDYiwPRASLQIAKRLIag 162
gi 27381523   126 QGVENVVK--GKIPYVYTPVFEGddcssnvyFLGEVPEQELQKTLPKMVelakgkTFYLVGNDYvwARTTNDKAKKYIad 203
gi 1487871     96 TAVAEALSd-APVLLVQPVMNEGgrgdrlrlQLGERPDDQLAAAVKPLMrmegvrRWFLAGDDYcwPRTTHGLARQLLpr 174
gi 119952742  191 AAVEDVLAa-SGLPVIHTVVNEGgggsdyvlRWGERPLSQVRAASDRVMkssggkRWYMIGNDYrwPRGAHRAGRRALte 269
gi 146339662  109 NAGLPIVAr-GKTPYIYTSFYEGkscspylyVNAWVPDQQVPPIVDYFNkekkakNYFLIGSDYafGRGMLAFTKMYIek 187
gi 117925926   99 IAVANMVAepRRIPHLNFSFYEGsifnpfffHFAALPNQQIKKMIPFMAnry-gvKMFFAGNNYewPRGSIDACKQSLek 177
gi 91781310   112 NAALPIVAr-GKKPLIYTSFYEGkscspwmyINGWVPDQQVAPVVDYFQktagakSFYLVGNDYafGRGMLDFTAKYIvl 190
gi 118043014  110 NAALPIVTr-GRIPFIYTSFYEGrscnkymyINAWVPEQQVAPMVDYFTrqkdakTFFLIGSDYafGRGMLAFTRKYIek 188
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 120401668  164 qgSPLVGQLYVplg--tedFTDAIRQVELTea-tGVVVLLVGgdGVAFNRQFAATGLdaaIPRLSPhveenmlmasgSQC 240
gi 56696374   187 agGEVVGEEYVpft--vseFDSSLQRIRESga-dAVLITLVGggSVGFNVSFAGFGLddqAIRLGTlieentlagigAEN 263
gi 148255810  163 vgCTVTGECYLpvg--ahdFDEMLDRIKITrs-dVVLPVFLGfdCIAFSRAFCAAGLsrhVLRFSSafdetivygldSSQ 239
gi 27381523   204 lgGTVVAEDYYplga-pnkFENTVAKIKSAkp-aIVLQTLVGgdNVNFNRTFADFNLsddIVRLSClleentligigKDA 281
gi 1487871    175 ygAGVVGEEFAslg--trdFAPIIESIQKSga-dIVLSTFVGadAVAFERQCYAMGLrdqCITLAPaleestltyigDEA 251
gi 119952742  270 agGHVAGNQFVplg--tqdFDSVIENIERSga-dCVLSTLIGsdEVAFERQVFTSGMrskWETLSLvleesarerigDEA 346
gi 146339662  188 tgGKVVGEEYLpmd--gsdWTSIISKLKASgadaLITSTAGGapNVTLTKQLRAAGV---NLPYGNlavdegtakgmGAD 262
gi 117925926  178 vgGECVGEEYLsigasaeeIENLLSKVARSga-dVFVPYFAGedQINLLNRFTELGLkkrMAVVMGhfdeamashlaPEV 256
gi 91781310   191 hgGKVVGEEYLpid--gtdWTPVLARIRAArpdaLISATAGGapNVALAKQLKASGL---TLPYGNlaidegtakgmGDV 265
gi 118043014  189 tgGKVVGEEYNpid--atdWTAIVSKIRAVhpdaLMISTAGGvpNVTLTKQLRTGGI---KALYGNlsidegtarsmGAD 263
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 120401668  241 NRNLFSAAGYFealnttasMEFAARYYHRFGlaap------alnsIGESCYEAIALLIalasRAGSLAIDALTSAAEylt 314
gi 56696374   264 ADRLYSSSGYFasikteaaASFAADYAAAFGgdga------alngLGQSAYEGLMLLAalanKAGSLDVAAMDAAAEgts 337
gi 148255810  240 TENLFVASSYFasmhsrnnGAFLERYYTAYGdnpp------ppngYGESCYEGVHALAalieRAGSFDARELRRYYGrtl 313
gi 27381523   282 SKNLYSSLGYFadldlpenKAFIGELTKKYGdkap------qqctISKGLFDAFVFAAavttKSKSLAAKEFAAAAEgvq 355
gi 1487871    252 ARGLYSVSGYFqglqtegnASLLKRYRAQFGpwap------plstLSESVFEAVHIWWqaarKAEDSERIAAAMRHGef- 324
gi 119952742  347 ARGIWTSLGYFegldtpenRVLVRRYRERYGrwap------plssLSESVYEAFMIYAtavrSSSPDTPSVIRALGQvsa 420
gi 146339662  263 AAGIYLSASYVtgidtpanKTFLASMAKKFGaelk------tpndLSVPQYEAVYAYKaaveKAGSTDQDKVLKALAevs 336
gi 117925926  257 REGFYSSNTYFmsvqtdlsKAYSERLIKLDQvkgihpdgnghitnFGEGTYLCVKAFAeaakVAGSLQPEDLIHALEhvq 336
gi 91781310   266 ATGIYLSGSYLtsintpenQKFLGEMQKRFGkdik------tpneLSEPQYEAFFLYKaaveKAGSTDPEKVIKALGqvs 339
gi 118043014  264 AVGTYVAGSYYtslqtpanQKFMAAMKARFGdkle------tpndLSVPEYDAVLAYAeavaKAGSTATDKVIPALAsvt 337
                         330       340
                  ....*....|....*....|....*...
Feature 1                                     
gi 120401668  315 -yvsPRGEVTMRGn--QMSQD-VYVAEA 338
gi 56696374   338 -wtsPRGANVLTGr--HMAQT-IYLADG 361
gi 148255810  314 qrrtARGVEAQPVv--GGRHP-VHLAEL 338
gi 27381523   356 -fetPSGSMTMTHr--HTAKN-IFLARC 379
gi 1487871    325 --qlPRGTVTLSEd--RLRQQ-LHLTKA 347
gi 119952742  421 --rmPRGLVASAGp-hALRQE-LYVARA 444
gi 146339662  337 -vdgPRGTIKMDKq-rHAPLT-MYLGQV 361
gi 117925926  337 -iesLQGSVRMDAkthHAHIN-SYLSCC 362
gi 91781310   340 -fdgPRGPVQMDKs--RHTPLaMRLGQI 364
gi 118043014  338 -vdgPRGAITMDKe--RHAALsMYLGQV 362

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