Conserved Protein Domain Family
PB1_Map2k5

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cd06395: PB1_Map2k5 
Click on image for an interactive view with Cn3D
PB1 domain is essential part of the mitogen-activated protein kinase kinase 5 (Map2k5, alias MEK5) one of the key member of the signaling kinases cascade which involved in angiogenesis and early cardiovascular development. The PB1 domain of Map2k5 interacts with the PB1 domain of another members of kinase cascade MEKK2 (or MEKK3). A canonical PB1-PB1 interaction, involving heterodimerization of two PB1 domain, is required for the formation of macromolecular signaling complexes ensuring specificity and fidelity during cellular signaling. The interaction between two PB1 domain depends on the type of PB1. There are three types of PB1 domains: type I which contains an OPCA motif, acidic aminoacid cluster, type II which contains a basic cluster, and type I/II which contains both an OPCA motif and a basic cluster. The Map2k5 protein contains a type I PB1 domain.
Statistics
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PSSM-Id: 99717
View PSSM: cd06395
Aligned: 5 rows
Threshold Bit Score: 165.044
Threshold Setting Gi: 115663051
Created: 27-Feb-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
PB1 interaction
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:PB1 interaction surface [polypeptide binding site]
Evidence:
  • Structure:2NPT; Human MAP2K5 and MAP3K3 form an asymmetric heterodimer
    View structure with Cn3D
  • Comment:Map2k5 contains a type I PB1 domain with a OPCA acidic amino acid motif while MEKK2/3 contains a type II PB1 domain

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       # # #        #                   
2NPT_A        15 LVIRIKIPNsGAVDWTVHSGPqLLFRDVLDVIGQVLPEattTAFEYEDEDGDRITVRSDEEMKAMLSYYYstvmeqqvng 94
1WI0_A        17 LVIRIKIPNsGAVDWTVHSGPqLLFRDVLDVIGQVLPEattTAFEYEDEDGDRITVRSDEEMKAMLSYYYstvmeqqvng 96
gi 115663051   6 LVIRIKTQHgEDVDWTVQLGQ-LSFHEVLEVIAQVLQDntpAAFEYEDEDGDRITVRSDDEMEAMINYYLsllaecearr 84
gi 147904898  17 LVIRIRIPDgGAVDWTVQPATqLLFRDILDVIGQVIPDattTAFEYEDEDGDRITVRSDEEMKAMLSYYCsmvleqqgsg 96
gi 166851860  17 IVIRIKTPV-GDMDSPVDSPShLNFNDLLAAIRDAMPEatvTAFEYEDEVGDRITVRSDDELKAMLSYYCntvmeqrfng 95
                         90
                 ....*....|.
Feature 1                   
2NPT_A        95 qliEPLQIFPR 105
1WI0_A        97 qliEPLQIFPR 107
gi 115663051  85 vmaPPLNIYPQ 95
gi 147904898  97 plmEPLQIYPR 107
gi 166851860  96 llpEPLQIFPR 106

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