Conserved Protein Domain Family
GH25_Cpl1-like

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cd06415: GH25_Cpl1-like 
Click on image for an interactive view with Cn3D
Cpl-1 lysin (also known as Cpl-9 lysozyme / muramidase) is a bacterial cell wall endolysin encoded by the pneumococcal bacteriophage Cp-1, which cleaves the glycosidic N-acetylmuramoyl-(beta1,4)-N-acetylglucosamine bonds of the pneumococcal glycan chain, thus acting as an enzymatic antimicrobial agent (an enzybiotic) against streptococcal infections. Cpl-1 belongs to the CP family of lysozymes (CPL lysozymes) which includes the Cpl-7 lysin. Cpl-1 has a glycosyl hydrolase family 25 (GH25) catalytic domain with an irregular (beta/alpha)5-beta3 barrel and a C-terminal cell wall-anchoring module formed by six similar choline-binding repeats (ChBr's). The ChBr's facilitate the anchoring of Cpl-1 to the choline-containing teichoic acid of the pneumococcal cell wall. Other members of this domain family have an N-terminal CHAP (cysteine, histidine-dependent amidohydrolases/peptidases) domain similar to that of the firmicute CHAP lysins and associated with endopeptidase activity. The Cpl-7 lysin is also included here as is LysB of Lactococcus phage, and the Mur lysin of Lactobacillus phage.
Statistics
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PSSM-Id: 119377
View PSSM: cd06415
Aligned: 11 rows
Threshold Bit Score: 234.985
Threshold Setting Gi: 90961782
Created: 13-Jun-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                         #                                 # #             
1OBA_A         6 DLFVDVSSHNGyditg--ileqmGTTNTIIKISESTTYl---------NRCLSAQVEQSNPIGFYHFARFGGDVAEAERE 74
gi 29374994   26 DQGVDWAIYQGeqgr----fgyaHDKFAIAQIGGYNASgiye--qytyKTQVASAIAQGKRAHTYIWYDTWGNMDIAKTT 99
gi 90961782   33 DHGVDAAVFQGasgk----wgyaRDKFMISQIGGTTTGwnlyd-qwtyPTQVSSTIAQGKRAHTYIWWQNVTSNSQADYV 107
gi 116618722  29 TNGIDVASYQGdtanyfnsfkqvGDNFTMVKLGGRGGGegahysnpkaYAQIHNADAVGMQTGGYFWAQVGDSVSEATYS 108
gi 116513255  38 SYGVDVSSYQPtstte---yskaGAQFVIVKVSEGTSYrnp-----naKAQVASAKANKLLPMAYHFANFGSSSSRAKSE 109
gi 42518242   47 STGVDVSSWQGtkldq---qaksGAQFAVVKVSEGTNYqnp-----naQGQIQSAEQNNMMTMGYHYTHFGSNSNQAVQE 118
gi 42518419    8 SYGVDISSFQGtdlla---mgrsGAKFAIVKTSEGLDYrnp-----kaQAQINSARNNGMMVSGYHYARFSNNANVAAQE 79
gi 76562539  154 DYFIDVSAYQQadltt--tcqqaGTTKTIIKVSESIAWl---------SDRHQQQANTSDPIGYYHFGRFGGDSALAQRE 222
gi 148750862   5 TYGVDVASYQStdmaa---yhkaGASFAIVKLTEGTDYvnp-----kaSKQVDSSRANHLYTHAYHFARFGSSVSQAKKE 76
gi 33321856   10 SYGVDVASYQSttv------nyaGAKFAFVKLTEGTEYtnp-----kaEAQIKSAKAHGLMVMGYFYANHSASVSKAMAE 78
gi 194466744   5 KTVIDLASFQShltvd--dykaiDADYAIVKTTESTNYvnp----yirSLIDRSAGGGIKGFAFYHFGRFHNDTQAVAEA 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                             # #                                            #           
1OBA_A        75 AQFFLDNvpm----qvKYLVLDYEddpsg--------------daqaNTNACLRFMQMIADAGYKPIYYSYKPFTHDNVD 136
gi 29374994  100 MDYFLPRiqt---pknSIVALDFEhgalasvpdgyggyvssdaekaaNTETILYGMRRIKQAGYTPMYYSYKPFTLNHVN 176
gi 90961782  108 LNYFLPKiqt---pkgSIVALDVEsg-------------------yqNTQAIAHAIQRIKDAGYTPMLYGYKNYLVNNTD 165
gi 116618722 109 AQLAVQDaqnaglakgSYIAMDYEagaga--------------nkanNTTAILAFMDQIYASGYKPMFYSYTSYVNSYVD 174
gi 116513255 110 ADYAIASakavslpagSYLACDWEtssgnst----------sgsrsaNTSAIMAFMKKVKAAGYQPLLYSGSYLLNNAIN 179
gi 42518242  119 GNYAVNSaqqaglpqgSYLATDWEqdvnnnt----------ngsvaaNTNAITSFMDTVHDGGYNPMLYSSEWLLKNKVD 188
gi 42518419   80 GNYAVSSakqvgiepgSYLACDYEqgsgnet----------ggnreeNTTAILAFLNTIVSAGYKPLLYSGAELMKNKVN 149
gi 76562539  223 ADLFLSNlps---kkvSYLVIDYEdsasa--------------dkqaNTNAVIAFMDKIASAGYKPIYYSYKPFTLNNID 285
gi 148750862  77 ATYFIKEakkedisqkRIVWLDWEsgsgntv----------tgskasNTSAILAFMDAIKAAGWRPGLYSGASLLRTAID 146
gi 33321856   79 AKYAVAKakaygipagSYLADDWEqgsgnsv----------ngstsaNTDAVLAAMQAIKEAGYKPLIYSGAYNLRNHLS 148
gi 194466744  79 NYFIANSktqanvqpgTLMILDAEis-------------------nmPTSSVIAFLNTVRNAGYHPGFYTYKYLLPNFNL 139
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1                    #                              #          #      
1OBA_A       137 YqqilaqFPNSLWIAGYglnd-----gtanFEYFPSMDGIRWWQYSSn----pFDKNIVLL 188
gi 29374994  177 YqqiikeFPNSLWIAAYpidgv---spyplYAYFPSMDGIGIWQFTSayiaggLDGNVDLT 234
gi 90961782  166 Lsy--lsTLCQLWLAEYpnyav---tpepnYNYFPSFNNIGIFQFTStyvaggLDGDIDLT 221
gi 116618722 175 LsrinarYPNALWLAWYlttah---qatppMQYFPNYSNVKIWQYADnh--ygVDGNVMVV 230
gi 116513255 180 MstvtstYPNSLWVAYYtssgr---idapdFSYFPSMNGVIIWQFTEnwkglnVDGNISVL 237
gi 42518242  189 TnkisekYPNALWVAKYktngr---ednpdYNYFPSMDNVAIWQYTQnwrgqnVDGNVNVV 246
gi 42518419  150 TakilakYPNCLWVAAYpykga---tntadFNYFPSMEGVIIWQFTDnwrgmnVDGNINLL 207
gi 76562539  286 YqkiiakYPNSIWIAGYpdyev---rteplWEFFPSMDGVRWWQFTSvgvaggLDKNIVLL 343
gi 148750862 147 TaqvvkkYGTCLWVASYptmaa---vssadFRYFPSMDGVAIWQFTSnwkglgVDGNLALV 204
gi 33321856  149 IsrivksFGTCLWVASYkvsgr---qdyadFNYFPSMDGVAIWQFTDnyrglgVDGNITLI 206
gi 194466744 140 Eai--hpNMDMFWLAAYplangeaagknpdFNYFPSANYVDMWQHTDnllgynVDGSITLT 198

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