Conserved Protein Domain Family
M1P_guanylylT_A_like_N

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cd06428: M1P_guanylylT_A_like_N 
N-terminal domain of M1P_guanylyl_A_ like proteins are likely to be a isoform of GDP-mannose pyrophosphorylase.
N-terminal domain of the M1P-guanylyltransferase A-isoform like proteins: The proteins of this family are likely to be a isoform of GDP-mannose pyrophosphorylase. Their sequences are highly conserved with mannose-1-phosphate guanyltransferase, but generally about 40-60 bases longer. GDP-mannose pyrophosphorylase (GTP: alpha-d-mannose-1-phosphate guanyltransferase) catalyzes the formation of GDP-d-mannose from GTP and alpha-d-mannose-1-Phosphate. It contains an N-terminal catalytic domain that resembles a dinucleotide-binding Rossmann fold and a C-terminal LbH fold domain. GDP-d-mannose is the activated form of mannose for formation of cell wall lipoarabinomannan and various mannose-containing glycolipids and polysaccharides. The function of GDP-mannose pyrophosphorylase is essential for cell wall integrity, morphogenesis and viability. Repression of GDP-mannose pyrophosphorylase in yeast leads to phenotypes including cell lysis, defective cell wall, and failure of polarized growth and cell separation.
Statistics
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PSSM-Id: 133050
View PSSM: cd06428
Aligned: 18 rows
Threshold Bit Score: 342.697
Threshold Setting Gi: 50422827
Created: 6-Jun-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative substrate binding site
Evidence:
  • Comment:Predicted GDP-glucose binding site based on similar proteins.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           ####               #                                                         
gi 62897461    4 AVILIGGPQKGTRFRPLSFEVPKPLFPVAGVPMIQHHIEACAqvpgmqEILLICFYqPDEPLTQFleaa---qqeFNLPV 80
gi 115899346   4 AVILIGGPQKGTRFRPLSLELPKPLFPVAGFPIIYHHIEACSklpdiqDVLLIGTYqPSDSLNRFitsa---qreFKFNI 80
gi 156394465   4 VVILIGGPMKGTRFRPLSLELPKPLFPVAGFPIIHHHIEACKkipdlkEVILIGFYqSSEPLSKFitsa---qqeFGLQI 80
gi 19527511    4 AVILIGGPQKGTRFRPLSLDTPKPLFPLAGRPLIAHHIEACAqlpdirEILIIGYY-PQTQMEGFvgdmqalyssSNINI 82
gi 50256873   30 GVILVGGPSKGTRMRPLTLDCPKPLLPVAGKPMIWHPLQALSkvpdltEVIIIGFY-DDSQMAAFvkea--krefPNIAI 106
gi 50553116    5 ALILVGGGTRGTRFRPLSLDTPKVLFPVGGKPILSHAVDAVAslkdvkEVLLVGFY-EDSVFSQFiadt--nkqyPNLSI 81
gi 85091191   22 AVILVGGASRGTRFRPLSLDVPKPLFDVAGHPIIWHCLTAIAkvpsihEVYLIGYY-DESVFRDFikda--vhefPQLTI 98
gi 3080527     5 AVILVGGPSRGTRFRPLSFDVPKPLFKIGGREMIYHHLAALSkiesvkDVFLVGFY-DESVFKDFineva-shfpSFNRI 82
gi 7331959     5 AVVLVGGPQKGTRFRPLSLQLPKPLFPIAGVPLIEHHIDQLCqlsglsEILLLGFF-PSDVFTDFisrc---qqtYRVSI 80
gi 198434056  10 AIILIGGPEKGTRFRPLSLDVPKPLFPVAGFPMVQHHIEACCqvpeikEILLIGFFpLSDELKSFleya---kkqFTPSI 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          ###                                           
gi 62897461   81 RYLQEFAPLGTGGGLYHFRDQILags-peaFFVLNADVCSDFPLSAMLEAHRRqr--hPFLLLGTtanrtqslnYGCIVE 157
gi 115899346  81 RYLQEYTALGTAGGLYHFRDQILsgq-pkgFLVFNGDVCCKFPLAELWDFHEKvsqddHYTMLSTeatrdqslsYGCLVE 159
gi 156394465  81 RYLQEYQPLGTVGGIYHFRDQISfgn-pdgLVVINADISCDFPFQELLEFHQQhp--gKHTIMTTeanrkqaynYGCLVE 157
gi 19527511   83 RYLQEFTALGTAGGMYHFRDQIRagn-praFFVLNGDVCADFPLQELCDFHEKrpasaLVTIMSTeatrqqslhYGCLVF 161
gi 50256873  107 SYLREYKALGTAGGLYHFRDSILrppvpqhIFICNIDICCSFPFAEMLELHTShg--gTGTIMGVnvkketatqYGCIVT 184
gi 50553116   82 KYLREYKAMGTAGGLYHFRDVILkgn-psrFFVIHADVCCSFPLKEIEEFYEEkk--aKYVILGTtvpaavannFGAIVT 158
gi 85091191   99 KYLREYQALGTAGGLYHFRDIILkgr-perLFVLNSDVCCSFPLGEMLRLFEEkd--aEAVILGTrvaedaatnFGCIVS 175
gi 3080527    83 KYLREYNCLGTGGGLYHFRDQILkgh-tsnVFVMHADVCCSFPLQELLNVHHEkk--aLVTLMATkvskedasnFGCLVE 159
gi 7331959    81 KYLEEPNPLGTAGGLVSFKKQILagd-pdaVFVINADVCGDLPIEDMGAKLDSls-gsSMLMLTTeatrqqsinFGSVVT 158
gi 198434056  87 RYLQEYTSLGTAGGLYHFRDQIGagn-psaFLVFNADVCCNFPLQEILSFHKSkttddGITIVATeatqqesmsYGCVVE 165
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              #                                                         
gi 62897461  158 NpQTHEVLHYVEKPSTFISDIINCGIYLFSPEALKPLRdvfqrnqqdgql----------------------edspglwp 215
gi 115899346 160 NeNTHEVMHYVEKPQTFVSTVINCGLYVFPPEIFQHIGkafqqnqdevl------------------------ngdplfp 215
gi 156394465 158 KeGSHEVVHYVEKPETFVSSLINCGVYIFSLTVFQTLSelmqhtyniar------------------------ddhvgfv 213
gi 19527511  162 DrSSGAVSHYVEKPSSYVSTFINCGVYVCSMDIFTVLAqifhsrgqeyscqaf-----------------cngngngngr 224
gi 50256873  185 DpETNQMVHYVEKPEGWISNIVNGGVYLFDKSLFDVIKvamdektaraa-------------------------edplvk 239
gi 50553116  159 DpETQRVIHYVEKPESHISNLINAGVYLFDQTIFDTIAaakkvreekaqd-----------------------psfvgeg 215
gi 85091191  176 DaHTRRVLHYVEKPESHISNLINCGVYLFATEAIFPSIrtaikrrtdrpnrlirypsaenlessffaqqaaddddeesek 255
gi 3080527   160 EpSTGRVLHYVDKPSSYLSNIISCGIYIFDASIFDEIKkayerrleevek----------------------qlrsldeg 217
gi 7331959   159 D-SEGRVIHYVDKPTTFVSTNISCGVYLIKAEVIRQLDlpln-------------------------------------- 199
gi 198434056 166 DaATGEATHYVEKPETFVSSLINCGVYAFSPKVLQYMEnvfkehqserfds---------------------gddnadqs 224
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*...
Feature 1                                                        
gi 62897461  216 gagtIRLEQDVFSALAG--QGQIYVHLTDGIWSQIKSAGSALYASRLY 261
gi 115899346 216 skdtIRLEQDILAPLAS--TGKLFTFKTNNFWSQIKSAGSAIFANRLY 261
gi 156394465 214 gpevLRMGEDLLTHLAG--TGELFAYKTDRFWTTIKSAGSAIYANRNF 259
gi 19527511  225 eqghIKWEQEVLTPLAG--TDKLFAMPVPNWWSQLKTAGSAIYANRHY 270
gi 50256873  240 pdeiLRLEQDVIVPLAA--ARKMYVYQTHDFWRQIKTAASAVTATALY 285
gi 50553116  216 dedhLRLEQDILVQLPA--TDAFYAYETKDFWRQIKTAGSAVPANALY 261
gi 85091191  256 rsevIRLEQDILSDMAD--SKQFFVYETQDFWRQIKTAGSAIPANALY 301
gi 3080527   218 medyLSLETDVLAPLCSdsSKAIYAYNTPEFWRQIKTAGSAVPANSLY 265
gi 7331959   200 -gdgIWLETDVLPQLAS--SGNLYALHTTRWWSQTKTAAAVLYANRHY 244
gi 198434056 225 fserISLEKEILNPYAG--SGKMFVYKTKEVWGQIKSAASAIHANKLY 270

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