Conserved Protein Domain Family
GT2_HAS

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cd06434: GT2_HAS 
Hyaluronan synthases catalyze polymerization of hyaluronan.
Hyaluronan synthases (HASs) are bi-functional glycosyltransferases that catalyze polymerization of hyaluronan. HASs transfer both GlcUA and GlcNAc in beta-(1,3) and beta-(1,4) linkages, respectively to the hyaluronan chain using UDP-GlcNAc and UDP-GlcUA as substrates. HA is made as a free glycan, not attached to a protein or lipid. HASs do not need a primer for HA synthesis; they initiate HA biosynthesis de novo with only UDP-GlcNAc, UDP-GlcUA, and Mg2+. Hyaluronan (HA) is a linear heteropolysaccharide composed of (1-3)-linked beta-D-GlcUA-beta-D-GlcNAc disaccharide repeats. It can be found in vertebrates and a few microbes and is typically on the cell surface or in the extracellular space, but is also found inside mammalian cells. Hyaluronan has several physiochemical and biological functions such as space filling, lubrication, and providing a hydrated matrix through which cells can migrate.
Statistics
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PSSM-Id: 133056
View PSSM: cd06434
Aligned: 24 rows
Threshold Bit Score: 253.331
Threshold Setting Gi: 46122895
Created: 12-Nov-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
DXD motif
Feature 1:DXD motif
Evidence:
  • Comment:DXD motif in the catalytic site could be in binding the metal ion that is used to corordinate the phosphates of the NDP-sugar in the active site.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 46107638   76 DVTVVIPTIHNafEELRPSLESILace--paELILVTTHDKRkelqkl-------------------------------- 121
gi 158318998  51 STSVIVPSFREdpDVLIRCLETWLsqq--pdEIIIIPDVEDTeliarl-------------------------------- 96
gi 16081925   37 DVTIVMPVYKEdpIVFEESVASASqq---gsQVYVLGDDCDEpyksi--------------------------------- 80
gi 48478040   45 DVTAVIPVYNEnpEIFESVIKSLInq---cgSLIVVGDSSYEpyrsi--------------------------------- 88
gi 197728829  51 TTSVIIPVVDEpeDLFRAVLQRIIqqq--ptEIIVVVNGRRNpdler--------------------------------- 95
gi 160937779  54 FTSVLLPVVDEplDLFYSVLMKIArqn--psEIIVVINGPKNeglenlcvd----------------------------- 102
gi 15898141   63 KVSAIVPEYGEnlEIFEKCLRSVAknk--pdEIIVVHDDKRKevvdi--------------------------------- 107
gi 4885391    84 TVALCIAAYQEdpDYLRKCLQSVKrltypgiKVVMVIDGNSEddlymmdifsevmgrdksatyiwknnfhekgpgetdes 163
gi 45382207   84 TVALCIAAYQEdpDYLRKCLLSVKrltypgiKVVMVIDGNSEddvymmdifteimgrdksatyiwsnnfhdkgpgetees 163
gi 24119268   84 SLALCIAAYQEdpNYLRKCLISVKrltypgiKVIMVIDGNNDddcymmeifreimgrdkaatyiwksnyhhrgpeetees 163
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                        ###                             
gi 46107638  122 --------adslvfPKVRVLDTPIANKRLQVCEAlpkv--etpITIMADDDVTWPStlmPWILAPFe-DPEIGGVGTCQR 190
gi 158318998  97 ---------aqradPTVRVIPFVHEGKRSALGVGlsaa--trdIVVLCDSDTAWEPgllAAVQMPFv-DPQVGGVGTRQN 164
gi 16081925   81 ----------aekhGCAFIRVSPHRGKRGILSYSmkiv--ktpLVMFLDSDTVLDHgavEDMIKYL--EEDVGGVGTNIS 146
gi 48478040   89 ----------tekfNGRFIYLKKHSGKRLALVEGiknv--ktrYVLFVDSDTLVPEkatLSMLSKF--KRNVGGVGVNIN 154
gi 197728829  96 ----------icsdLDVMWIWTETPGKRNALRVGlgha--tgeIAVLVDSDTVWTRntlSELVKPFk-DAKVGGVTTRQR 162
gi 160937779 103 -----fnrnlpicfTPVQHYYTPVAGKRNGIRVAmehinpnsdITVLVDSDTVWTEdtlSELLKPFacDQKIGGVTTRQK 177
gi 15898141  108 ----------skkyGAKVISLSRRVGKRGALIIGwlna--igdIIVQLDSDTIMEDntiNEIVKPFa-DPKVAGVQGRPV 174
gi 4885391   164 hkessqhvtqlvlsNKSICIMQKWGGKREVMYTAfralgrsvdYVQVCDSDTMLDPassVEMVKVLeeDPMVGGVGGDVQ 243
gi 45382207  164 hresmqhvsqlvlsNKSVCIMQKWGGKREVMYTAfkalgeawnYVQVCDSDTMLDPassVEMVKVLeeDPMVGGVGGDVQ 243
gi 24119268  164 yatslqhvshlvlnNKCVCIMQKWGGKREVMYTAfkalgrsvdYVQVCDSDTMLDPassVEMVKVLeeDPNVGGVGGDVQ 243
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 46107638  191 VRRehdgswstKAWNWLGAAYIERrnfeis-athnidGGTSCMSGRTGAYRSEILsshdflhgfknekwrkwilNADDDN 269
gi 158318998 165 VYEpr-----sSVWRRVANWLVDIryldyv-paqgrvGAVACLSGRTAAYRRSAIlpvlhnleh--efflgrrcIAGDDG 236
gi 16081925  147 IFRn-------GHPVSYTSEFVERvrevif-ksmsrkSSVMVLDGRCALYRTNLVmpfltsrefqdntvmgkksVLGDDR 218
gi 48478040  155 IKNd-------GSGIAYASEFIERvrevif-ramsyhGSILVADGRCAMYLTEAVrplllsndfmnkkilgkrtMLGDDI 226
gi 197728829 163 ILEp------gRSVLTRWADWLESvrcqyslpamsvmGTVGCLPGRTIAFRKQILddcmedfls--erflgvflEVSDDR 234
gi 160937779 178 ILDp------dRKLVTMFANLLEEiraegtmkamsvtGKVGCLPGRTIAFRTQILidvmydfmn--etfmgfhkEVSDDR 249
gi 15898141  175 LFRtd----grIPYLFGQIIEYSRdivv-----ralnGTLNVIDGKIAAYRRSYLletirhfnh--etygkrklIAADDK 243
gi 4885391   244 ILNky-----dSWISFLSSVRYWMafnier-acqsyfGCVQCISGPLGMYRNSLLhefvedwyn--qefmgnqcSFGDDR 315
gi 45382207  244 ILNky-----dSWISFLSSVRYWMafnier-acqsyfGCVQCISGPLGMYRNSLLhefvedwyn--qefmgsqcSFGDDR 315
gi 24119268  244 ILNky-----eSWVSFLSSVRYWMafnier-acqsyfGCVQCISGPLGMYRNSLLhefledwyd--qtfmgshcSFGDDR 315
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*..
Feature 1                                                       
gi 46107638  270 FVTRWlvshQWKTWIQYEKeceiettlensTKFLYQCSRWARSNWRS 316
gi 158318998 237 RLTWLvlasGYKTMHQHTAhamsm-fpdnlRAFIKQRVRWSRNSYRT 282
gi 16081925  219 QLTSYiirsGYRAVKDYDVyarty-qyssfKKFFKQQIRWGRVGWTY 264
gi 48478040  227 QITSYliksGYKVVKDYNVtvqte-pqgdfKRFFRQQIRWSRNGWYY 272
gi 197728829 235 TLTNYtlraGYRTVYQSSSlvytd-aplelGKLARQQYRWARGSQYN 280
gi 160937779 250 SLTNLtlrkGYKTVMQDTSviytd-aptewKKFIRQQLRWSEGSQYN 295
gi 15898141  244 ALTYYanmnGYKTVYQATAvaksa-aqptfLKFLNQQLRWARSGYLY 289
gi 4885391   316 HLTNRvlslGYATKYTARSkclte-tpieyLRWLNQQTRWSKSYFRE 361
gi 45382207  316 HLTNRvlslGYATKYTARSkclte-tpieyLRWLNQQTRWSKSYFRE 361
gi 24119268  316 HLTNRvlslGYATKYTARSkclte-tpityLRWLNQQTRWSKSYFRE 361

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