Conserved Protein Domain Family
Trp-synth_B

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cd06446: Trp-synth_B 
Click on image for an interactive view with Cn3D
Tryptophan synthase-beta: Trptophan synthase is a bifunctional enzyme that catalyses the last two steps in the biosynthesis of L-tryptophan via its alpha and beta reactions. In the alpha reaction, indole 3-glycerol phosphate is cleaved reversibly to glyceraldehyde 3-phosphate and indole at the active site of the alpha subunit. In the beta reaction, indole undergoes a PLP-dependent reaction with L-serine to form L-tryptophan at the active site of the beta subunit. Members of this CD, Trp-synth_B, are found in all three major phylogenetic divisions.
Statistics
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PSSM-Id: 107207
View PSSM: cd06446
Aligned: 50 rows
Threshold Bit Score: 478.569
Threshold Setting Gi: 13878841
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
catalyticpyridoxal
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Structure:1V8Z_A; catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                         #             
1BEU_B       23 PALNQLEEAFVRAqkdpEFQAQFADLLknyaGRPTALTKCQNITagt--rttLYLKREDLLHgGAHKTNQVLGQALLAKr 100
1V8Z_A       17 EPLKELEKAYKRFkddeEFNRQLNYYLktwaGRPTPLYYAKRLTekig-gakIYLKREDLVHgGAHKTNNAIGQALLAKf 95
gi 7674373   57 FAKELIKQEFSEKry-iKIPKEVRELYak-iGRPTPLFRATNLEkilktparIYFKYEGATVtGSHKINTALAQAYYAKk 134
gi 7674388   48 FPMSLIEQEVSEErf-iEIPEPVLKEYa--vYRPTPLIRATFLEeylqtparIYYKYEGVSPtGSHKPNTAIAQAYYNKi 124
gi 7674395   51 FPKALIEQEVSREry-iEIPGEVHEAYisfaRRPTPLLRAVNLEralntpaeIYYKYEGVTPtGSHKINTALAQAYYNKl 129
gi 13878841  44 VPAKVLEYEFSGEry-pKIPDEILERYmq-vGRPTPIIRAKKLEellggnlkIFLKMESYTYsGSHKINSALAHVFFARe 121
gi 14424473  44 LPKEVLRQQFTIEry-iKIPEEVRDRYls-iGRPTPLFRAKRLEeylktparIYFKYEGATPtGSHKINTAIPQAYFAKe 121
gi 21673033  50 FPMNLIEQEVSTErw-iDIPEEILGILk--lWRPSPLYRARRLEaalgtpakIYYKNEGVSPaGSHKPNTAVAQAWYNRe 126
gi 24212536  59 FPKALIMQEMSSDry-iDIPEEVLDVYr--lWRPSPLFRAHQLEkvlkspakIYYKYEGVSPaGSHKTNTSIAQAYYNMk 135
gi 24212562  36 LPSALIDQEFTAErw-vSIPEEVREAYrr-vGRPTPLFRAEGLEkalgtgvrIYYKYEGVLPvGSHKLNTALAQAYYAKa 113
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1BEU_B      101 MGKSEIIAETGAGQHGVASALASallgLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGs---------------aT 165
1V8Z_A       96 MGKTRLIAETGAGQHGVATAMAGallgMKVDIYMGAEDVERQKMNVFRMKLLGANVIPVNSGs---------------rT 160
gi 7674373  135 EGIKRLVTETGAGQWGTALSLAGaligLKVRVYMTRASYYQKPYRKILMEIYGAEVFPSPSEntevgkrflredpnhpgS 214
gi 7674388  125 EGVKRLVTETGAGQWGSALSYAGakfgLEVKVFMVKVSYQQKPMRKYMMNLFGGKVTPSPSEetnfgrkilsedpdnpgS 204
gi 7674395  130 EGVERLVTETGAGQWGSALSAAGayfgVKVRVYMVRVSYLQKPYRRTLMELYGAEVYPSPSDktefgrkllaenpnhpgS 209
gi 13878841 122 EGAKFVSTETGAGQWGSAVALASalfhMESHIFMVRTSFYAKPYRKYMMYMYGAHPHPSPSEfteygrevlkrmpdtpgS 201
gi 14424473 122 EGIEHVVTETGAGQWGTAVALAAsmynMKSTIFMVKVSYEQKPMRRSIMQLYGANVYASPTNlteygrkiletnpqhpgS 201
gi 21673033 127 FGIKYLTTETGAGQWGSALAMSCkligIECKVFMVRISFDQKPFRKIMMNTWGAECIPSPSPltavgrrileedpdtpgS 206
gi 24212536 136 EGTERLTTETGAGQWGSALSLACnyfdLECKVYMVRSSFYQKPYRKSLITLWGGNVVPSPSPdtefgrkilqeqpdtpgS 215
gi 24212562 114 DGAVEVATETGAGQWGMAVSLAAalfgLRAVVFMTRSSYNSKRQRLTFMRAYGAVVYPSPSDvteagrrh--yrpdhpgS 191
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1BEU_B      166 LKDACNEALRDWSGSyeTAHYMLGTAagphpypTIVREFQRMIGEETKAQILDkegrlPDAVIACVGGGSNAIGMFADFI 245
1V8Z_A      161 LKDAINEALRDWVATfeYTHYLIGSVvgphpypTIVRDFQSVIGREAKAQILEaegqlPDVIVACVGGGSNAMGIFYPFV 240
gi 7674373  215 LGIAISEAIEDVLSDe-KARYSLGSVl------NHVLMHQTVIGLEAKKQVKEfe--ePDVIIGCVGGGSNFAGLSYPFI 285
gi 7674388  205 LGIAISEALEVAVSDp-NTKYSLGSVl------NHVLLHQTVIGLEIKKQLELig-ekPDILLGCHGGGSNFGGTILPFV 276
gi 7674395  210 LGIAISEAIEDVINSggNAKYSLGSVl------NHVLLHQTVIGLEAEKQFREag-vyPDIMIGAVGGGSNFAGFTYPFI 282
gi 13878841 202 LGLAISEAIHYALDN--GGKYIAGSVi------NSDILFKTIAGMEAKKQMEMag-edPDYIVGVVGGGSNYAALAFPFL 272
gi 14424473 202 LGIAMSEAIEYALKN--EFRYLVGSVl------DVVLLHQSVIGQETITQLDLlg-edADILIGCVGGGSNFGGFTYPFI 272
gi 21673033 207 LGIAISEAIEQAVERd-DTRYALGSVl------NHVMLHQTIIGLEARKQFDKig-ryPDIVIGCAGGGSNFAGISFPFL 278
gi 24212536 216 LGIAISEAVEDAIAHe-NTKYSLGSVl------NHVVLHQTVIGAECKQQLAQve-eyPDVVIGCCGGGSNLGGIGLEFI 287
gi 24212562 192 LGIAISEAVEYVLSGe-RRKYLPGSVm------EFVLLHQTVIGLEAVRQLPEe----PDVAVACVGGGSNFAGFTYPMI 260
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1BEU_B      246 NDt-------sVGLIGVEPGGhgietgehgaplkhgrvgiyfGMKAPMMqtadgQIEESYSISAGLDFPsvGPQHAYLNS 318
1V8Z_A      241 NDk-------kVKLVGVEAGGkglesgkhsaslnagqvgvfhGMLSYFLqdeegQIKPTHSIAPGLDYPgvGPEHAYLKK 313
gi 7674373  286 KDvldg--kaeYEFIAVEPRAvptmtkgvytydygdsagltpKIKMYTLg---hTYYVPPIHAGGLRYHglAPTLSVLMN 360
gi 7674388  277 PDkls---grdIRFVACEPAAcpsltkgnydydfgdtagltpLLKMYTLg---kDFIPPKIHAGGLRYHgsAPIIARLVK 350
gi 7674395  283 RHrlkg--sssTRFIAVEPKAspsmtrgvytydygdtagltpLLKMHTLg---hTYQVPPIHAGGLRYHgvAPTLSVLLK 357
gi 13878841 273 ADelss-gkirRTYIASGSKEvpkmtegeyrydypdtgkvlpLLKMYTIg---yDFIPPAVYAGGLRYHavAPTLSLLMN 348
gi 14424473 273 GNkk------gKRYIAVSSAEipkfskgeykydfpdsagllpLVKMITLg---kDYVPPPIYAGGLRYHgvAPTLSLLTK 343
gi 21673033 279 YDkih---gkdVQVIATEPEAcptltrapyaydsgdvammtpLLPMHSLg---hTFIPPAIHAGGLRYHgmAPLVSHTKQ 352
gi 24212536 288 KDrleg--khsARVVAVEPSAcpsltkgeyrydfgdtaemtpLLKMYTLg---hKHVPPAIHAGGLRYHgdSPIISKLCS 362
gi 24212562 261 GMklrgegfgrTRFVAVESEAapkltrgeykydfpdavgvlpMLKMYTLg---hDYVPPAIHAAGLRYHgaAPSLSLLRK 337
                       330       340       350       360       370       380       390
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1                                                                              
1BEU_B      319 iGRADYVSITDDEALEAFKTLCRhEGIIPALESSHALAHALKMmreqp--ekeqLLVVNLSGRGDKDIFTV 387
1V8Z_A      314 iQRAEYVTVTDEEALKAFHELSRtEGIIPALESAHAVAYAMKLakem---srdeIIIVNLSGRGDKDLDIV 381
gi 7674373  361 hGIVKPMAYHQTEVFEAAVLFAKaEGIVPAPESAHAVRAVIDKaleakrkgeetVILFNLSGHGLLDLKGY 431
gi 7674388  351 eGLVEAQAFDQDETFEAAKIFAKlEGIIPAPESAHAIAGAIREakkakeegkerVIVFTLSGHGLLDLTAY 421
gi 7674395  358 hGIVEARAYHQREVFRAAHMFAKaEGIVPAPESAHAVKAAIDEaikardegrrvVIAFNLSGHGLLDLQGY 428
gi 13878841 349 kGIVSARDYDQEEAFKWARIFSEtEGYIPAPETSHALPILKEIadknr--gekkTVLVSFSGHGLLDLGNY 417
gi 14424473 344 eGIVEWREYNEREIFEAAKIFIEnQGIVPAPESAHAIRAVVDEaiearknnerkVIVFNLSGHGLLDLSNY 414
gi 21673033 353 lGLIEATALPQTECYEAALLFAHtEGFIPAPETSHAIAQTIREakqakeegkekVILMNWSGHGLMDLQGY 423
gi 24212536 363 eGLMEAVSYDQQEVFDAAVQFARtEGIVPAPESSHAIRCAIDEalaakqtgeekTILFNLSGHGHFDMSSY 433
gi 24212562 338 lGIVEAVAYPQEEVMRAALLFARtEGIVPAPESAHAIKAAVDLakkl---prgsVIAFNLSGHGLLDSDAY 405

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