Conserved Protein Domain Family
SepCysS

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cd06452: SepCysS 
Click on image for an interactive view with Cn3D
Sep-tRNA:Cys-tRNA synthase. This family belongs to the pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). Cys-tRNA(Cys) is produced by O-phosphoseryl-tRNA synthetase which ligates O-phosphoserine (Sep) to tRNA(Cys), and Sep-tRNA:Cys-tRNA synthase (SepCysS) converts Sep-tRNA(Cys) to Cys-tRNA(Cys), in methanogenic archaea. SepCysS forms a dimer, each monomer is composed of a large and small domain; the larger, a typical pyridoxal 5'-phosphate (PLP)-dependent-like enzyme fold. In the active site of each monomer, PLP is covalently bound to a conserved Lys residue near the dimer interface.
Statistics
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PSSM-Id: 99745
View PSSM: cd06452
Aligned: 11 rows
Threshold Bit Score: 626.726
Threshold Setting Gi: 11497798
Created: 23-Jun-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2E7J_A         9 FINIDPLQTGGKLTEEARQALLEWGDGYSVCDFCtt-gRLDEIKtPPIHDFIHNQLPKFLgcdVARVTNGAREAKFAVMH 87
gi 116664877  28 EINLDPLQRGGILTPEARAALVEWGDGYSVCDFCe--gMLDRVRnPPVEEFVHEILPRFLdvdDVRITHGAREGMYAAMH 105
gi 1500582    34 FINLNPIQRGGILPKEAKKAVYEYWDGYSVCDYCh--gRLDEVTcPPIKDFLEDIAKFLNm-dCARPTHGAREGKFIVMH 110
gi 20089607   21 SINIDPLQTGGLLTEAARQALVEWGDGYSVCDFCg--gVLDQIKkPPIHDFVHKALPEFLgcdEARVTNGARESKFAVMH 98
gi 72396310   21 SINIDPLQTGGRLTEAAKQALVEWGDGYSVCDFCg--gILDQIKkPPIYDFVHKALPEFLdcdEARVTNGARESKFAVMH 98
gi 15679078   16 NLNLNPLQRGGVLPAAARKALHEFGDGYSVCDYCd--gRLDQVTrPAINCFLDDLADFTGs-dAVRTVHGAREGKFAVMH 92
gi 19886544   17 YINVNPIQRGGVLTPEARKALLEFGDGYSVCDFCe--gLLHEIEkPPIRQFHEDLAEFLGm-dVVRITAGARYAKEAVMS 93
gi 11497798   15 MINVHPIQRGGILTEEARKVLLEWGDGYSMCDICle-gRVDLLDkPPVKRFKEEVAEFLGm-dEVRFTAGARHAKFVAMS 92
gi 91772810   83 FINIQPIQAGGRLTPEARKAVIAYGDGYSTCDNCrkpfRLDKIEkPSISTFHTDLAEFVGm-dQARVVPGARRGFQAVAS 161
gi 126179560  16 YINIDPIQAGGRLTVDAMKAAISFADGYSVCDNCrspfRLDYIQkPPIAQFHADLAAWLNm-dTARVVPGARRGFQAVAS 94
gi 124485175  16 SINLDPIQVGGRLTPEAMKAMISWGDGYSVCDNCrkpfRLDYIEkPPLKDFHVDVAEWLGm-aQARTVPGARRGFQQVAG 94
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2E7J_A        88 SLAkkdAWVVMDENCHYSSYVAAERAglNIALVPktdypd-yaitpENFAQTIEETkkr--geVVLALITYPDGnyGNLP 164
gi 116664877 106 SLCdagDYIVADGNAHYTTVLAAERAglEISYVEnsgepe-ytinpEGYREAIRDAadq-gyrVSMAVLTYPDGnyGNLV 183
gi 1500582   111 AICkegDYVVLDKNAHYTSYVAAERAklNVAEVGyeeeyptykinlEGYKEVIDNLedk-gknVGLILLTHVDGeyGNLN 189
gi 20089607   99 SMGkpgDWIVLDGLAHYSSYVAAERAglNVKVVPhagspe-yyldpEGYGTAIEEVtkesgkpPVLALVTYPDGsyGNIP 177
gi 72396310   99 SIGkpgDWIVLDGLAHYSSYVAAERAglNIKTVPhsgspd-yyldpEGYATAIEEVtkesgkpPALALVTYPDGsyGNLP 177
gi 15679078   93 ALCergDTVVVDGNAHYTTHLAAERNglEIVEVPstghps-yevtpEAYREVLEETidr--veVKLAVLTHVDGnyGNLT 169
gi 19886544   94 ALCeegDVVVADSLAHYTTFVAAEKAgaTVREVPntghpe-ykvkvDEYARVIDEVedergdpPALALLTHVDSeyGNLA 172
gi 11497798   93 GFK---GALVVDSLAHYTTYIAAELNglRVYEVPntgype-frieaESYTDVFEKVkeetgdyPAVALLTHADYkyGNLA 168
gi 91772810  162 SIIekgDTVVVSTFAHYTEFLAVEGAggIVREAPvnehn---iltaESVAHKIDEVkretgklPALIMIDHFDYlfCNEH 238
gi 126179560  95 TYVekgDPVIVTSLAHYTEFVAVEEAggIPLEVPkdekn---hitpDAAAEKIEAVilefsktPPLLFIDHVDYqfGNVH 171
gi 124485175  95 TYVekgDPVLIGALAHYTSYLSVELQkgIVREIPktadn---hitaEDTANRIEDVvrefgiaPKLLYIDHVDYqfGNMH 171
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                    #                                   
2E7J_A       165 DVKKIAKVCSeydVPLLVNGAYAIGrMPVSLkeigADFIVGSGHKSMAAsgPIGVMGMKEEWAEIVLRrsek-------- 236
gi 116664877 184 DARRVAEICHeegVPLLLNGAYSIGrMPVSAreldADIVVGSGHKSMASsgPIGVLGAKQEYADIIFRrsks-------- 255
gi 1500582   190 DAKKVGKIAKekgIPFLLNCAYTVGrMPVNGkevkADFIVASGHKSMAAsaPCGILAFSEEFSDKITKtsek-------- 261
gi 20089607  178 DAGKIASVCHeyeVPLLLNGAYCVGrMPVSAkeigADFIVGSGHKSMAAsgPVGVLGVSEEYAPIVFRksvy-------- 249
gi 72396310  178 DARKIASVCHeydVPLLLNGAYAVGrMPVSAkeigADFIAGSGHKSMAAsgPVGVLGVSEEYAPIVFRksky-------- 249
gi 15679078  170 DARGVADVCRklgVPLLLNCAYSMGrLPVNLrelgVDFVVGSGHKSMAAsgPIGVLGMKSEWEDTVLRrsgr-------- 241
gi 19886544  173 DAEKFVKICRkkgVPALLNCAYTMGrMDLSNlspkPDFMVGSGHKGMAAcaPCGVLAMREEWEEEVLRgsslrgdvsgre 252
gi 11497798  169 DAKKVAEICRdygIPLILNTAYTSGiMEVSGretgCDFIVASGHKSWAAtaPIGILATTFEFAERVFRvsevrgnwsgra 248
gi 91772810  239 DIYGIGKVAQeygIPFLYNGAYTVGiMPVNGqkigADFVVGSGHKSMASaaPSGVLATTEEWADKIFRttqmvgdvtgrk 318
gi 126179560 172 DVAAITKVAHqydIPVLVNGAYSVGiMPVDGkalgADFVVGSGHKSMAApaPSGVLATTNEHAERVFRttqakgdvtgrt 251
gi 124485175 172 DVKGIAKVAHqydIPVLYNGVYTVGiMPVNGkdlgVDFIIGSGHKSMAApaPSGILAATEERANEVFRttqmegdltgrk 251
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2E7J_A       237 yknkEVELLGCTArg-aTIITLMASFPHVRERikrWDEEVEKARRFAAEMEKL-GIKQLGDNPHNHDLMFFHAe-VLYEI 313
gi 116664877 256 kknkEVELLGCTVrg-aGLLTMMASFPHVLERvgrWQEEVDKARWFSSELEEL-GIEQQGEKPHNHDLMFFKSe-RLYEI 332
gi 1500582   262 fpvkEIEMLGCTSrg-lPIVTLMASFPHVVERvkkWDEELKKTRYVVDELEKI-GFKQLGIKPKEHDLIKFETp-VLDEI 338
gi 20089607  250 skvkEVELLGCTArg-aTVMTMIASFPEVVKRvrnWDQEVENARWFSARLEDM-GFIQRGQKPHSHDLMFFEAp-GFYEI 326
gi 72396310  250 nkvkEVELLGCTArg-aTVMTLIASFPDVVKRvrnWDQEVENARWFSARFEEM-GFIQRGQKPHSHDLMFFEAp-RFYEI 326
gi 15679078  242 hekkELELLGCTSrg-aPLATLMASLPYVRERvsrWDGEVKKTRYLVSELEDIgGIEQLGVRPKEHDLVRFETp-VFHEI 319
gi 19886544  253 wphkEVEMLGCTVmg-aPIVTMMASFPHVVERvkrWKEEVRKTRWFVKEMERIeGVRQLGERPKRHDLVKFETp-GFHEV 330
gi 11497798  249 ftkkEVALFGCSPvyglPLVTLMASFPVVKERvkrWKEEVEKARWFVEEMEKIeGVMLLGERPKNHTLMNFETp-SFNLI 327
gi 91772810  319 fgikEVEFLGCTLmg-aPLLSMMASFPHVKERtkhWDEEVKKSNYFINEFLRIeGNEVLSEFPRKHALSKVDTtgSFDKV 397
gi 126179560 252 fgikEVEMMGCTLmg-vTVVGMMASFPHVKERvkhWDTEVAHSQAVVDALLSIeGTKVLSDYPRQHTLTRIDTreSFDKV 330
gi 124485175 252 fgikEVGILGCSLmg-aPIVGLLASFPTVKARvehFDEELANSKIVVEALRFIeGTKILSEYPRKHTLTRVDTtgSFDQV 330
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*
Feature 1                                                               
2E7J_A       314 SKkakggrffLYRELKSRKIHGikpgltRYFKLStyglsDEEVDYVLNAFKEIIE 368
gi 116664877 333 SKsarkgryfLYHELKDRGICGikpgltRQFKLStylipREDLERVVSAFHEIIE 387
gi 1500582   339 AKkdkrrgffFYDELKKRGIGGiragvtKEIKMSvygleWEQVEYVVNAIKEIVE 393
gi 20089607  327 SQkikkgryfLYKELKERNIHGiksgltKYFKLStfglgKEKLGVVADSFEEILK 381
gi 72396310  327 SEkvkkgryfLYKDLKARNIHGiksgltKYFKLStfglgKEKLGIVADSFEEILK 381
gi 15679078  320 AAshprkgffLYEELKKRGIVGirrgqtKWFKCSiygmtEEQVQYVVDSFRDIVE 374
gi 19886544  331 AEdhprrgyfLYEELKKRGVIGiqpgqtETIKASvygltDEQVEHVVRAFHEIAE 385
gi 11497798  328 AKkhrrkgyfLYHELKERGIFGvqpgmtRNVKLNvyglsWEEVERVAEAFKEIAE 382
gi 91772810  398 AKthkrkgyfFSDELKKRGIAGefpgatRSWKMStyglsWDQIHYLSNSFIEIAD 452
gi 126179560 331 AQqhkkrgffLSSDLKKRGITGvipgstRVWKFNtfgltEKQIRHVGESFVEIAR 385
gi 124485175 331 AQthkkrgffLSSALGKKGITGiipgatKVWKFNtygmtKKQAEYVADTYLEIAE 385

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