1FC4,2JGT,1BS0,2BWO


Conserved Protein Domain Family
KBL_like

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cd06454: KBL_like 
Click on image for an interactive view with Cn3D
KBL_like; this family belongs to the pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The major groups in this CD corresponds to serine palmitoyltransferase (SPT), 5-aminolevulinate synthase (ALAS), 8-amino-7-oxononanoate synthase (AONS), and 2-amino-3-ketobutyrate CoA ligase (KBL). SPT is responsible for the condensation of L-serine with palmitoyl-CoA to produce 3-ketodihydrospingosine, the reaction of the first step in sphingolipid biosynthesis. ALAS is involved in heme biosynthesis; it catalyzes the synthesis of 5-aminolevulinic acid from glycine and succinyl-coenzyme A. AONS catalyses the decarboxylative condensation of l-alanine and pimeloyl-CoA in the first committed step of biotin biosynthesis. KBL catalyzes the second reaction step of the metabolic degradation pathway for threonine converting 2-amino-3-ketobutyrate, to glycine and acetyl-CoA. The members of this CD are widely found in all three forms of life.
Statistics
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PSSM-Id: 99747
View PSSM: cd06454
Aligned: 35 rows
Threshold Bit Score: 382.679
Threshold Setting Gi: 28900566
Created: 17-Jun-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Structure:2BWO_B; 5-Aminolevulinate Synthase catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1FC4_A        45 SHVINFCANNYLGLANh--pDLIAAAKAGXDSHGfgxasvr-----ficgtqdSHKELEQKLAAFLgXEDAILYSSCFDA 117
gi 21672892   51 RKLLNLSSNDYLGLGAdrelFSSFIAQIRDDRFDdgrfamtssssrlltghhpVCDQLESAIAAAYgSETALVFNSGYHA 130
gi 27377208   31 RVGIDFASNDYLALGNa--sRIKKAMVAALEAGTpvgaggsr----llrgnceEHERLETEAAQFFgAEAALFFGGGYIA 104
gi 27467099   35 QSYINFTSNDYLGIGQl--eYQPQNFLDFIKTYSihlsssr-----lvsgnsvVYQQLEQAISEHFnFEDALIFNSGYDA 107
gi 30250226   37 RDFLAFCSNDYLGLANh--pALIEAAAEGARCYGvgsgash-----lisghfrAHHELEEALAAFVgLPRTLLFSTGYMA 109
2JGT_A        65 KDTILLGTYNYMGMTFd--pDVIAAGKEALEKFGsgtngsr-----mlngtfhDHMEVEQALRDFYgTTGAIVFSTGYMA 137
gi 29826641  886 RELLSFSSYNYLGMATh--pQVNEAARKAIERCGtsvsasr-----llsgsrpLHLELEAELAATLgCEAAITLVNGHAT 958
1BS0_A        39 RQYLNFSSNDYLGLSHh--pQIIRAWQQGAEQFGigsggsg-----hvsgysvVHQALEEELAEWLgYSRALLFISGFAA 111
gi 19704184   31 KSLYNFSSNDYLSLAHdkdlLQKFYQNYNFDNYKlsssssr-----lidgsylTVMRLEKKVEEIY-GKPCLVFNSGFDA 104
2BWO_B        46 QDITVWCGNDYLGMGQh--pVVLAAMHEALEAVGagsggtr-----nisgttaYHRRLEAEIAGLHqKEAALVFSSAYNA 118
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1FC4_A       118 NGGLFETLl--gAEDAIISDALNHASIIDGVRlckAKRYRYANndxqeLEARLkeare-agarHVLIATDGVFSXDGVIA 194
gi 21672892  131 NTGILPALa--tRHDLILSDRLNHASIIDGLRiadADYRRFRHadydhLEEQLetaar-ecyrQIFIVTESVFSMDGDLA 207
gi 27377208  105 NFALLTTLp--qRGDLLVLDSLVHASIHEGARagrAECRIHAHndpqsVEDAIrawrnkggagRVWIVAESLYSMDGDFA 182
gi 27467099  108 NLAVFNIFk--nNNVVIFSDQQNHASIIDGIKlsgLSKVIYQHlnyddLESHLaqdt--hldvQKVIVSDSVFSTNGTKA 183
gi 30250226  110 NMAVVTALa--gRGDAIFADRLNHASLNDAALlsrARFIRYPHldldtLARQLett----karRRLVVTDAVFSMDGDMA 183
2JGT_A       138 NLGIISTLa--gKGEYVILDADSHASIYDGCQqgnAEIVRFRHnsvedLDKRLgrlp---kepAKLVVLEGVYSMLGDIA 212
gi 29826641  959 NVTVIGHLv--gEGDLIVHDSLAHDSIIQGCRlsgARRRPFPHndaaaLDALLtqvr--hhyrRVLVVAEGVYSMDGDIA 1034
1BS0_A       112 NQAVIAAMm--aKEDRIAADRLSHASLLEAASlspSQLRRFAHndvthLARLLasp----cpgQQMVVTEGVFSMDGDSA 185
gi 19704184  105 NSSVIETFf--dKKSLIITDRLNHASIYEGCInsrAKILRYKHldvsaLEKLLkkys--enynDILVVTETVYSMDGDCA 180
2BWO_B       119 NDATLSTLrvlfPGLIIYSDSLNHASMIEGIKrnaGPKRIFRHndvahLRELIaadd---paaPKLIAFESVYSMDGDFG 195
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                               #                        
1FC4_A       195 NLKGVCDLADkydALVXVDDSHAVGfvgengrgs---heycdvxgrVDIITGTLGKALGgasGGYTAARkEVVEWLRQRs 271
gi 21672892  208 DLRRLVKLKRrfkAVLIVDEAHGAGvfgerglgl---cealgvtneIDIIVGTFGKSLAs-aGAYAVMRgLFREYLVNTm 283
gi 27377208  183 PLEELVAIADrqdAFLIVDEAHATGayghqgrgl----tapydkreNLIVVHTCGKALGa-aGALVTASgILRDFMVNRc 257
gi 27467099  184 DINRLVHLKQrynAILIIDASHSLGlnlfe------------yhadIDIVTSSLSKAWGa-hGGIIFSSkDIKDLIINKg 250
gi 30250226  184 PVAELLTLCQrfdAWLLLDDAHGFGvlgergkgslyhsqrierdtpYLIYMATLGKAAGv-sGAFVAAQaPVVETLIQHg 262
2JGT_A       213 PLKEMVAVAKkhgAMVLVDEAHSMGffgpngrgv---yeaqglegqIDFVVGTFSKSVGt-vGGFVVSNhPKFEAVRLAc 288
gi 29826641 1035 DLPALIEVKRrhgALLMIDEAHSIGvigaagrgig--qyfdvdrqdVELWSGTLSKALAs-cGGYVAAGrTVVDYLRYTv 1111
1BS0_A       186 PLAEIQQVTQqhnGWLMVDDAHGTGvigeqgrg-----scwlqkvkPELLVVTFGKGFGv-sGAAVLCSsTVADYLLQFa 259
gi 19704184  181 EIKQICDLKEkynFNLMVDEAHSYGaygygiay------neklvnkIDFLVIPLGKAGAs-vGAYVICDeIYKNYLINKs 253
2BWO_B       196 PIKEICDIAEefgALTYIDEVHAVGmygprgagv---aerdglmhrIDIFNGTLAKAYGv-fGGYIAASaRMVDAVRSYa 271
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1FC4_A       272 rpylfsnSLAPAIVAASIKVLEXVea--gsELRDRLWANARQFREQXSAagfTLAga--dHAIIPVXLgdaVVAQKFARE 347
gi 21672892  284 rtlifttALPPMTLAWSLATFTKQlt--mqRERDHLLGLAATLRGSLREagfDVPge--sHIVPVVLGe-dRTAVEMAGA 358
gi 27377208  258 rpfifatAPSPLMAVAVREALLTLqk--epERQQRLAKLVAFAHRQITSlglRTPst--sQIVPYIVGd-nAHAMRLASA 332
gi 27467099  251 rsliyssSLPSYHLYFIQVSLQHVie--dtYRREKLNALSEYFNHQFMElfpNQPls--nTPIKNIVCdslASAQAQYDM 326
gi 30250226  263 rtygyttAAPPLLAHTLLTSLQLIsq--esWRRERLALLIERLRQRLHSlpwPLLls-etPIQPLLVGe-sQEAVRLDLA 338
2JGT_A       289 rpyiftaSLPPSVVATATTSIRKLmt--ahEKRERLWSNARALHGGLKAmgfRLGtetcdSAIVAVMLedqEQAAMMWQA 366
gi 29826641 1112 pgfvfsaGMTPANTAASLAALRVLra--epQRVARLKENSALFARLAHRagiDTGps-hdTPIIPCIVgdsAKTLQLAEA 1188
1BS0_A       260 rhliystSMPPAQAQALRASLAVIrsdegdARREKLAALITRFRAGVQDlpfTLAds--cSAIQPLIVgdnSRALQLAEK 337
gi 19704184  254 kkfiystALPPVNNLWNLFVLENLvn--fqDRIEKFQELVTFSLNTLKKlnlKTKst--sHIISIIIGd-nLNAVNLSNN 328
2BWO_B       272 pgfifstSLPPAIAAGAQASIAFLktaegqKLRDAQQMHAKVLKMRLKAlgmPIIdh--gSHIVPVVIgdpVHTKAVSDM 349
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*...
Feature 1                                                        
1FC4_A       348 LQke-gIYVTGFFYPVVPkgqARIRTQXSAAHtpEQITRAVEAFTRIG 394
gi 21672892  359 LRea-gFHALPVRPPTVPensSRLRFSLRADLtsGDIAALAETMKRGA 405
gi 27377208  333 LQar-gFDIRGIRPPTVPagtARLRISLTLNVgeADVRAMLDALVEVT 379
gi 27467099  327 LFeh-gIFVSYLRYPTVSq--PTLRISLSYFHdtDDIDRLFNVMKQYD 371
gi 30250226  339 LRer-gIWVPAIRPPTVPqgmARLRISLSAVHteADVDRLGAALRDLA 385
2JGT_A       367 LLdg-gLYVNMARPPATPagtFLLRCSICAEHtpAQIQTVLGMFQAAG 413
gi 29826641 1189 LFeq-gISVNPILYPAVPekaARLRFFITCDHtpDQIHHTVRELAHGL 1235
1BS0_A       338 LRqq-gCWVTAIRPPTVPagtARLRLTLTAAHemQDIDRLLEVLHGNG 384
gi 19704184  329 LKel-gYLAYAIKEPTVPkdtARLRISLTADMkkEDIETFFKTLKAEM 375
2BWO_B       350 LLsdygVYVQPINFPTVPrgtERLRFTPSPVHdlKQIDGLVHAMDLLW 397

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