Conserved Protein Domain Family
GT_MraY-like

?
cd06499: GT_MraY-like 
Glycosyltransferase 4 (GT4) includes both eukaryotic and prokaryotic UDP-D-N-acetylhexosamine:polyprenol phosphate D-N-acetylhexosamine-1-phosphate transferases. They catalyze the transfer of a D-N-acetylhexosamine 1-phosphate to a membrane-bound polyprenol phosphate, which is the initiation step of protein N-glycosylation in eukaryotes and peptidoglycan biosynthesis in bacteria. One member, D-N-acetylhexosamine 1-phosphate transferase (GPT) is a eukaryotic enzyme, which is specific for UDP-GlcNAc as donor substrate and dolichol-phosphate as the membrane bound acceptor. The bacterial members MraY, WecA, and WbpL/WbcO utilize undecaprenol phosphate as the acceptor substrate, but use different UDP-sugar donor substrates. MraY-type transferases are highly specific for UDP-N-acetylmuramate-pentapeptide, whereas WecA proteins are selective for UDP-N-acetylglucosamine (UDP-GlcNAc). The WbcO/WbpL substrate specificity has not yet been determined, but the structure of their biosynthetic endproducts implies that UDP-N-acetyl-D-fucosamine (UDP-FucNAc) and/or UDPN-acetyl-D-quinosamine (UDP-QuiNAc) are used. The eukaryotic reaction is the first step in the assembly of dolichol-linked oligosaccharide intermediates and is essential for N-glycosylation. The prokaryotic reactions lead to the formation of polyprenol-linked oligosaccharides involved in bacterial cell wall and peptidoglycan assembly. Archaeal and eukaryotic enzymes may use the same substrates and are evolutionarily closer than the bacterial enzyme. Archaea possess the same N-glycosylation pathway as eukaryotes. A glycosyl transferase gene Mv1751 in M. voltae encodes for the enzyme that carries out the first step in the pathway, the attachment of GlcNAc to a dolichol lipid carrier in the membrane. A lethal mutation in the alg7 (GPT) gene in Saccharomyces cerevisiae was successfully complemented with Mv1751, the archaea gene.
Statistics
?
PSSM-Id: 133460
View PSSM: cd06499
Aligned: 10 rows
Threshold Bit Score: 142.055
Threshold Setting Gi: 15678761
Created: 8-Jan-2008
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
Mg++ bindingputative
Feature 1:Mg++ binding site [ion binding site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                      ##                
gi 61214073   70 GVPTMGGLlillsvtvstllwar-----------------ldnpliwlvllvtlFFGMIGAYDDykkiskkt-------- 124
gi 156357179  48 PIPEGLGAvggalflicmflfiplpflsiwlekgdfdfphhefvmfiaallsicCMIFLGFADDvldl------------ 115
gi 116754254  41 KVPEMGGVplvfgfvlgvlvyigietf---------ylnsyrytpilaalctvlMACIIGIMDDilgwka---------- 101
gi 121534916  44 PIPRLGGLaiylgfvlavlasm------------------hvnreimgllmggtVILVVGIIDDlkql------------ 93
gi 145219422  52 PTPRIGGLaifaalfvawlllpk-----------------gsgmllglmliaslPAFVAGFIEDltkr------------ 102
gi 20094190   43 GMPIMGGLgillgtasgplla---------------------siqalppiasalPMMVLGIVDDllglqveefqkvvrnv 101
gi 15678761   45 GTPTMGGLgmllalllvtvlyr-------------------nnpylvltslivlTAAIVGLLDDllglkvkevqriirnv 105
gi 148642420  51 GTPTMGGIaflfvilliiaiyy-------------------nntpilimsyimlAGGIVGLLDDliglkvkeiqkvvvnc 111
gi 84490006   47 GTPTMGGFgiligvsvmalflfd----------------whstsyllitvimmvVAGIFGMFDDllgfkvkeyqkvvrnd 110
gi 75491838   44 PTPRGGGVaivisfligiilfyfl--------------gylpilsvvglivsggVIALVGFWDDhghi------------ 97
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 61214073  125 ------------------------------------------------seglsakGKLLLQIAGALIvgffvylhpgydg 156
gi 156357179 116 ----------------------------------------------------kwrDKLILPTMASLPllmvyfvnigvtt 143
gi 116754254 102 --------------------------------------------------glrqwQKPMFMLFAAMPmmvinaghttmsl 131
gi 121534916  94 ----------------------------------------------------parVKLLGQIVAAMVlllfnvriewltn 121
gi 145219422 103 ---------------------------------------------------vgvrERLLATILSGVAayyltgysltrvd 131
gi 20094190  102 snrpielgrlilqpgeearvatekakrdlkrileedpdaieivgevpikkelserEKLALQTGAALFvlpivpatwlwip 181
gi 15678761  106 segplevgqlvlkpgeear--aatdkakrdveallseglvevvgeapiknevsegEKILAQLLIGVFlvlsgavgklgg- 182
gi 148642420 112 cdevitlgrldvepneevr--vatpkakkeidklveegkveiigevpiktepgelEKIVFQILLGVLlgltgvittigg- 188
gi 84490006  111 gdkpvsiglltlepgeear-iasekakadynkiveqehkltlideipiksettesEKIITQFVVACFvilpgflptsvs- 188
gi 75491838   98 ----------------------------------------------------aarWRLLAHFSAAAFllfcfggfpvlnv 125
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 61214073  157 qlsip---fmknvqpdlgwfyivfaVIVIVGASNAVNLTDGLDGLAAGPMVVSSAvyllfaylagnvvlanylhipyvag 233
gi 156357179 144 iivpkpvrfifgfdldlgilyyvymGMLAVFCTNAINILAGINGVEAGQSLIIAMsivlfnllelqgtccwq-------n 216
gi 116754254 132 pl--------igrvdwgilyplviiPIGVVGASNAFNMVAGYNGLEAGMGVIIFAalgyaglvmgkts------------ 191
gi 121534916 122 pf---------gdmiyldyfsipltILWVVGLTNTVNLIDGLDGLAAGVSTIASItillvalqqnfwt------------ 180
gi 145219422 132 vpg-------fdvllqwmpftvaftAFAVGGVANSVNIIDGFNGLAGGVLMICFGmlaaiawqvgdth------------ 192
gi 20094190  182 k----------vgvwnlsylfypvaVFGIVGATNAVNLIDGMDGLAAGLLFLASLacsgvclgqgqlp------------ 239
gi 15678761  183 --------------fylglaaapiaIAGMVGAINAVNLIDGMDGMAAGIMLIASLscaiflglsgqa------------- 235
gi 148642420 189 --------------fnlgilafpvvIIAILGCINCVNLIDGMDGLAAGIVGIASFacccylylcgnmg------------ 242
gi 84490006  189 -------------gfelgilmiplaLFAIIGSINSVNLIDGMDGLAAGIIAIASIasalyanvttgsv------------ 243
gi 75491838  126 s----------gfiielgifgslfgLLFLVWMLNLYNFMDGIDGLASAEAVTACIgmiaiyyisgdhiel---------- 185
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
Feature 1                                                      
gi 61214073  234 sgelaifcgtlfgaclGFLWFNAHPAqmFMGDVGSLALGGALGSIA 279
gi 156357179 217 hlfslyfmlpfiavcsALLYYNWYPSsiFVGDTFCYFAGMAFAVVG 262
gi 116754254 192 ---aaalsiimlgsllAFLYFNRYPArvFPGDTMTYSVGALAACVA 234
gi 121534916 181 ---vailtaalagsalGFLYHNFNPAkiFMGDTGSMFLGYMLAAVS 223
gi 145219422 193 ---laklcilfilcvaGFMFLNFPFGkiFMGDGGAYLMGFLLAWTA 235
gi 20094190  240 ---gaaffaslagaslGFLFHNRYPAkiFMGDTGSCFLGAAYAAGA 282
gi 15678761  236 -----lpflalagmcaGFLVFNRHPAsiFMGDTGSFALGAGYATAV 276
gi 148642420 243 ---gmpvfiiltglclGFLVFNKYPAsiFMGDTGSFVLGTGYAAAV 285
gi 84490006  244 ---aalpfiiiagacvGFLVLNHYPAkiFMGDTGSYALGTGYMTAA 286
gi 75491838  186 --nsflvlwllactvlGFLLWNFPPAkiFMGDAGSGFLGLMIGSLA 229

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap