Conserved Protein Domain Family
GH25_PlyB-like

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cd06523: GH25_PlyB-like 
Click on image for an interactive view with Cn3D
PlyB is a bacteriophage endolysin that displays potent lytic activity toward Bacillus anthracis. PlyB has an N-terminal glycosyl hydrolase family 25 (GH25) catalytic domain and a C-terminal bacterial SH3-like domain, SH3b. Both domains are required for effective catalytic activity. Endolysins are produced by bacteriophages at the end of their life cycle and participate in lysing the bacterial cell in order to release the newly formed progeny. Endolysins (also referred to as endo-N-acetylmuramidases or peptidoglycan hydrolases) degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
Statistics
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PSSM-Id: 119383
View PSSM: cd06523
Aligned: 10 rows
Threshold Bit Score: 210.296
Threshold Setting Gi: 116334530
Created: 25-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                         #                                       # #        
2NW0_A         2 YIVDMSKWngspdw---dtakgQLDLVIARVQDGSny-----------vDPVYKDYVAAMKARNIPFGSYAFCRFvsvED 67
gi 30020403    3 YIVDISKWngtinw---diaasQLDLVIARVQDGSnt-----------vDFMYQNYVSEMKKHSIPFGNYAFCRFisiAD 68
gi 22533771   67 PIIDVSGWqlpkeid-ydtlskNISGVVIRVFGGSkisktnnaayttgiDKSFKTHIKEFQKRNIPVAVYSYALGssvKE 145
gi 15673122   63 PVVDLSGWqrpedid-yntlsqHVIGAVIRVNGSYghadns--askdgeDTAYKQHIKAFQERGIPTAVYAFVTGentSE 139
gi 158321460   1 MIIDISSHqvpanmn-ydelakQVKLVIIRTQYGSrl-----------iDKHYKTHHREFKKRGIPTAAYAWVRGtsiED 68
gi 28377547  429 AIVDVSEFqsdinw--sqvrnaGLALGVIRIQDGEny-----------iDVKHVKNLQGVLANNLNYAVYAFFRGvneAD 495
gi 90961774   45 KIIDVSEWqgvidw--nkviadDVTLSIIRVQHGSah-----------qDLKYMENLQQCISAGGKYAVYAYFAAtstSD 111
gi 28378444   29 AVPDISEWqgqltstevqslksQADFVINRVQYGSsy-----------eDLYHTSNESLYVKYGVPFGSYDYATFtstAT 97
gi 70727628   77 RVLDISEWqgnltdsqvkqlkkDYDFIIIRGQYGSey-----------vDQCLENNSALLDKNNMKFGVYSYSLYenaDD 145
gi 116334530  36 KIADISQYqgninw---skaskDLKYAIIRVQHGSkgda------gymvDSKRNVNANGAYKYGVPFGQYMFAEFtsvKD 106
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                   # #                                  #               
2NW0_A        68 AKVEARDFWNRgdk------dslFWVADVEvttm----sdmRAGTQAFIDELYRLga--kKVGLYVGHHKyeefga-aqi 134
gi 30020403   69 AKKEAQDFWNRgdq------sakFWVADVEvqtm----admQGGIQAFIDELRRLga--eKVGLYVGHHTylsfga-cni 135
gi 22533771  146 MKEEAQIFYKNaapy-----kptFYWIDVEeetm----snmNKGVQAFRKELKRLga--kNVGIYIGTYFmteqgi-svk 213
gi 15673122  140 MRKQARDFYRRaspy-----kptYYWLDVEvtnm----knmNQGIEAFRSELEKQga--kNIGIYAQDWFlrdnqi-kvd 207
gi 158321460  69 MRVEATDFYNRtkef-----nptIWFIDVEeqsm----ddmSSGVSAYVKTLRDLga--kKVGIYIGHHLyktfnl-nle 136
gi 28377547  496 SQAEARAFYQRvqnvvkgqqqprFYALDIEaien----nnmRGTVNAYISQLNDLgilnsQIVLYIANHLyqqlnl-dtt 570
gi 90961774  112 AQQEARDFYNRtqkvvagkqqpiFYAIDVEsiemsgdvtqmRAGVEAYMSQLNTLgvpdnKIVLYIANHLydkfnl-nva 190
gi 28378444   98 AKAEADKFYQRank------ntkFYVLDFEttsms--staaNAAVKAWYTEMRSLts--kKLIFYSYQSFattyantarq 167
gi 70727628  146 ARNEAQTLYSRapk-------asFYVNDFEqnsit--sgdaDTATKAWADEMKQLsg-nkKVLFYSYENFmtnnvpnavs 215
gi 116334530 107 AQQEAKDFYKRsna------htkFFVLDEEkrmss-aksseQTYVNAWLKTMRSLtd--kPLIMYAGEYYanahnl-dfs 176
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
Feature 1             #                        #              #    
2NW0_A       135 kcDFTWIPRYGakp---------aypCDLWQYDEYGQVPGig-kCDLNRL 174
gi 30020403  136 eaDFVWIPRYGgnkp--------aypCDMWQYADSGNVPGig-kCDLNRL 176
gi 22533771  214 gfDAVWIPTYGsdsgyyeaapqtelkYDLHQYTSQGYLPGfnqpLDLNQI 263
gi 15673122  208 kfKAIWIAAYGrntgywdaspettlsYKMQQFTDQGTLPGysgnVDLNMV 257
gi 158321460 137 evDAVWIPHYGrndgtln--stpiypCDIHQYTDKGKLNGysgyLDLNRL 184
gi 28377547  571 kvGSIWIPSYGtkp---------rypYDLWQYTDKGTLAGiptkVDMSQD 611
gi 90961774  191 rpGAIWIPSYGqndgtlanslkpthpYDLHQYTSKGSVNGitgnVDMSAE 240
gi 28378444  168 sfDAQWIANYSskp---------tisFSLWQYSSSYYLSSlgqyVDNSLY 208
gi 70727628  216 ayDGYWLAAYQeqep--------nreKVLWQYTDSYYAPElnqnVDANYI 257
gi 116334530 177 kfDGSWIAKYSstkps-----vsgtsIDLWQYTSSGSVSGisgsVDLNKT 221

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