Conserved Protein Domain Family
CIDE_N_CAD

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cd06535: CIDE_N_CAD 
Click on image for an interactive view with Cn3D
CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and the release of active DFF40/CAD nuclease.
Statistics
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PSSM-Id: 119368
View PSSM: cd06535
Aligned: 6 rows
Threshold Bit Score: 135.238
Threshold Setting Gi: 14278226
Created: 21-Feb-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
CAD/ICAD
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:CAD/ICAD heterodimer interaction site [polypeptide binding site]
Evidence:
  • Structure:1IBX_A; Homo sapiens DFF40 (CAD) and DFF45 (ICAD) heterodimerize.
    View structure with Cn3D
  • Comment:Although the CAD and ICAD CIDE-N domains are structurally similar, they bind one another asymetrically using different surfaces from each protein.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1             #        ## ##         #   #                                            
1IBX_A         4 KPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFqlperGSRLCLYEDGTELtEDYFPSVPDNAELVLLTLGQAWQG 80
1F2R_C         7 QPKCVKLRALHSACKFGVAARSCQELLRKGCVRFqlpmpGSRLCLYEDGTEVtDDCFPGLPNDAELLLLTAGETWHG 83
gi 187608292  11 KIRCYKIRTLHSQQKYGIACKNLAELKQKACRKFqldcpTTCVCLYEDGTEVsEEYLETVPDNTVLLLLTPGQTWQG 87
gi 35903034    6 KPKLVKIRSANQARKYGIAAVNLKELIKKGCQLLkvsssGVLVCLYEDGTVVtEEYFQNLPDNTDLVLLPHGQSWNG 82
gi 47222792    4 KEKPVKIRSYNSSNKYGVTAKNVKELLDKGCKLLkvppaGARICLYSDGTEFtEKYFHTLPAHTELVLLSRGQSWSG 80
gi 45383564    4 PLRGFRLRRPGSAQKFGAAAGSLRGLLRKGCRLLqlplaGSRLCLYEDGTELsEAFFRTLPPQTELVLLRPGESWPG 80

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