Conserved Protein Domain Family
GH18_EndoS-like

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cd06542: GH18_EndoS-like 
Click on image for an interactive view with Cn3D
Endo-beta-N-acetylglucosaminidases are bacterial chitinases that hydrolyze the chitin core of various asparagine (N)-linked glycans and glycoproteins. The endo-beta-N-acetylglucosaminidases have a glycosyl hydrolase family 18 (GH18) catalytic domain. Some members also have an additional C-terminal glycosyl hydrolase family 20 (GH20) domain while others have an N-terminal domain of unknown function (pfam08522). Members of this family include endo-beta-N-acetylglucosaminidase S (EndoS) from Streptococcus pyogenes, EndoF1, EndoF2, EndoF3, and EndoH from Flavobacterium meningosepticum, and EndoE from Enterococcus faecalis. EndoS is a secreted endoglycosidase from Streptococcus pyogenes that specifically hydrolyzes the glycan on human IgG between two core N-acetylglucosamine residues. EndoE is a secreted endoglycosidase, encoded by the ndoE gene in Enterococcus faecalis, that hydrolyzes the glycan on human RNase B.
Statistics
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PSSM-Id: 119359
View PSSM: cd06542
Aligned: 23 rows
Threshold Bit Score: 188.741
Threshold Setting Gi: 29349395
Created: 1-Jun-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #                                     #                                    
1EOM_A         9 GVCIAYYITDgrn-------------ptFKLKDIpdKVDMVILFGLKywslqdttklpggtgmmgsfksykdlDTQIRSL 75
gi 29346458   54 PKAFGWFGNWtgeg----------aslvNSMAGIpdSVDVVSIWGNWsnit------------------eaqkKDLKFCQ 105
gi 160886098  57 PVAFGWFGNWtgng----------atleKCLAGLpdSVDFVSIWGNWrnlt------------------eaqtKDLRYVQ 108
gi 29350117   53 EISYAYYEAWspiegvtg--ykdpaswgERMVGLpdSIDIVNLWMGIptaet----------------hpiayADMQYCQ 114
gi 60682876   78 PIMFGWFNAWqpdg----------agkyPRLSLLpdSMDVVSIWGNWhsls-------------------eekIKELRSV 128
gi 160887541  56 AICFGWYAGYtseas---------psqgLHFTGLpdSLDIVSLWSGIpsnnpayvets----yynerylptayEEMNYIR 122
gi 544222     61 QISAGYYRTWrdsat--------asgnlPSMRWLpdSLDMVMVFPDYtppena--------------ywntlkTNYVPYL 118
gi 29346454   71 VKGFGWFGNWtgkg----------nnaqNYLKMLpdSVDFVSLWGTRgyls------------------deqkADLKFFQ 122
gi 531383     61 PIFFGYYRTWrdkaiqlkdddpwkdklqVKLTDIpeHVNMVSLFHVEdnqksdq------------qfwetfhREYQPEL 128
gi 29346448   60 KIMFGWFGNWagkgg---------ssmqYALCGLpdSTDFVSLWLCWgnlt-------------------veqQADLKDF 111
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1EOM_A        76 QSRGIKVLQNIDDdvswqss---------------------------------kpggfaSAAAYGDAIKSIViDKWKLDG 122
gi 29346458  106 EVKGTRFTMCFIItsvgtqitpqhiydnwesmgfasqqeavndfwgwpsdesdkeaveaSIRKYANAIADTI-NKYGYDG 184
gi 160886098 109 NVKGTKALMCFIVqnigdqltpeeykd------------------nylefwgwnenkeeAIKKYAHAICDSI-DKYGYDG 169
gi 29350117  115 RKLGTRFVMHADAshyrhqftvdgv-----------------------dydlsqnkddeAMAAYAKWIVNQV-LEPGLDG 170
gi 60682876  129 QAKGTKVIIGWIIedigdqikwg--------------------------rdqwpaddtqAIKEYAQAIVDTI-NKYGYDG 181
gi 160887541 123 TVKGTRVVMCTICrigste----------------------------------fpktdaGIEAYALHLVRCV-LRNDLDG 167
gi 544222    119 HKRGTKVIITLGDlnsattt---------------------------------ggqdsiGYSSWAKGIYDKWvGEYNLDG 165
gi 29346454  123 EVKGGKALLCWIIqdlgdqltpkglnat-----------------qywveekgqgnfieGVKAYANAICDSI-EKYNLDG 184
gi 531383    129 KKRGTRVVRTVGAqlllnkikdkn-------------------------lygkhveddyKYREIARDVYNEYvVKHNLDG 183
gi 29346448  112 QAKGSRAVLCWRAgdigdnltpggnddavk-------------eafwgfdpkdeqscieAAKKYALAIVDTC-NKYNIDG 177
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         # # #                                                                          
1EOM_A       123 ISLDIEHSgakpnpiptfpgyaatgyngwysgsmaatpaFLNVISELTKYFgtta-----------------pnnKQLQI 185
gi 29346458  185 FDIDYEPNygnkgni------------------vddddrMFIFVDELGKHFgpks-----------------gtgKLLII 229
gi 160886098 170 FDIDYEPNfghrgnm------------------sgsdenMLLFIQTLGERIgpks-----------------gtdRLLVI 214
gi 29350117  171 VDVDWEGWsg---------------------------sdLVRLIKELGKYFgpeg----------------eqpdKLLIV 207
gi 60682876  182 FDYDYEPSyaspfkpgnhcgn------ltscsrdynkekEILFMKTMRELLgp---------------------dKLFHL 234
gi 160887541 168 LDLDYEPEgdwl-----------------------qndnFTKFIKVLGNYFgpqs-----------------gtgKLLIV 207
gi 544222    166 IDIDIESSpsga-----------------------tltkFVAATKALSKYFgpks-----------------gtgKTFVY 205
gi 29346454  185 FDIDYEPGyghsgtlany-----------qtispsgnnkMQVFIETLSARLrp--------------------agRMLVM 233
gi 531383    184 LDVDMELRqv---------------------------ekQLNLKWQLRKIMgafselmgpkapanegkkpdhegyKYLIY 236
gi 29346448  178 FDYDIEDWgtlmns--------------------smpsvPNAFMKTLREEFdk--------------------tgKMLVA 217
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                    # #                                                 
1EOM_A       186 ASgidvyawn---kimenfrNNFNYIQLQSYGanvsrtqlm--------------------mnyatgtnkIPASKMV-FG 241
gi 29346458  230 DGepqsik------nrpdvgPYFDYFIIQAYKpgndnnldkrlidggvagpglvqtygsvmseeqitkmtIMTENFEaVD 303
gi 160886098 215 DGepqsi--------vsesgPYFNYFIVQAYDspgdntgrdnldsrl---------nstirnfdghltaeEVAKKYI-VT 276
gi 29350117  208 DYfsgtp--------ptdiiPYCDYIVQQAYSdqvgfl---------------------------tqpsnFPPEKMI-YC 251
gi 60682876  235 NGsihwl--------dpraaQYFDRFVVQSYNgsassferw--------------------tndiqnrlnIKPEQLV-FT 285
gi 160887541 208 DFynqlp--------pketePYVDYFVRQCYTtgsaqtlqs---------------------kfdqlsswCPPEKFV-AT 257
gi 544222    206 DTnqnptn------ffiqtaPRYNYVFLQAYGrsttnltt----------------------vsglyapyISMKQFL-PG 256
gi 29346454  234 DGqpdll--------stetsKLVDHYIYQAYWesstssvi----------------------ykinkpnlDDWERKTiIT 283
gi 531383    237 DTfdnaqts-----qvglvaDLVDYVLAQTYKkdtkesvtq---------------------vwngfrdkINSCQFM-AG 289
gi 29346448  218 DIpggagwlsfyevlseetvLSLDYIAWQTYElghsgldsffe----------------avrrshpnvfkEAMGKSI-VT 280
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                              #                         
1EOM_A       242 AYAEggt--------------------nqaNDVEVAKWTPTQg----AKGGMMIYTYNSNv---------SYANAVRDAV 288
gi 29346458  304 AAMDggypytd------------rygnsmkSLEGMARWQPKNg---fRKGGVGSYHMEAE----------YPTNPEYKNL 358
gi 160886098 277 ENFEkwalsggadf-------tdrygnkmkSLEGMARWTPIIdgkkcVKGGVGTYHMEYEytidgteesyPYLRNAIRIM 349
gi 29350117  252 ESFGvfya-------------------dggQLMNYARWEPSKg----RKGGCGVFYLGRNy---------YSSSGIPYNE 299
gi 60682876  286 ESFQnkpg------------------arsrFPGTYAGYVASKq---gNVGGIGVFHINEDa---------FEDEAYVNIR 335
gi 160887541 258 EQMGwhwqnggvafteadgnkvdswgnpiySVIGMARWNPTQg----RKGGFGGYYFEYEynt-----trPANQAIGDKE 328
gi 544222    257 FSFYeengypgnywn-----dvrypqngtgRAYDYARWQPATg----KKGGVFSYAIERDa---------PLTSSNDNTL 318
gi 29346454  284 VEFEqgwktggityyts-vrpelnsmegnqILDYATLDLPSGk----RIGGIGTYHMEYDy---------PNDPPYKWLR 349
gi 531383    290 YAHPeendtnrfl--------tavgevnksGAMQVAEWKPEGg----EKGGTFAYALDRDg--------rTYDGDDFTTL 349
gi 29346448  281 ATFErav--------------------dkhYFTEQQSYHPACg---iEHAGMGAYHIEYDy---------PGNPDYPTVR 328

                 .
Feature 1         
1EOM_A       289 K 289
gi 29346458  359 R 359
gi 160886098 350 N 350
gi 29350117  300 F 300
gi 60682876  336 K 336
gi 160887541 329 K 329
gi 544222    319 R 319
gi 29346454  350 K 350
gi 531383    350 K 350
gi 29346448  329 A 329

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