Conserved Protein Domain Family
GH18_PF-ChiA-like

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cd06543: GH18_PF-ChiA-like 
Click on image for an interactive view with Cn3D
PF-ChiA is an uncharacterized chitinase found in the hyperthermophilic archaeon Pyrococcus furiosus with a glycosyl hydrolase family 18 (GH18) catalytic domain as well as a cellulose-binding domain. Members of this domain family are found not only in archaea but also in eukaryotes and prokaryotes. PF-ChiA exhibits hydrolytic activity toward both colloidal and crystalline (beta/alpha) chitins at high temperature.
Statistics
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PSSM-Id: 119360
View PSSM: cd06543
Aligned: 37 rows
Threshold Bit Score: 265.31
Threshold Setting Gi: 53803113
Created: 1-Jun-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2DSK_A; the catalytic domain of the hyperthermophilic chitinase from Pyrococcus furiosus binds glycerol, contacts at 4.5A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #                                    #                                     
2DSK_A        10 HFFAPYIDMSLSvhkplv----------eyakltGTKYFTLAFILyss-vyNGPAWAGsipl---------ekfVDEVRE 69
gi 499712     32 TEAAPYFFTWGYgessykfts-----lvdakqkaGLDAATLAFIItgs-sgCSLSNDVdn-------------mLSDVLA 92
gi 134100422  32 IRTAPYVDITREsptlpe-----------iaqatGQKHFTLAFVLgss-agCDPQWGGqiplt-------epriVDQVTR 92
gi 52842430   27 PIFSPYVDLTINthwdsqsqdmepmdlispakklGIKAYHLAFITds--gqCQPAWGAqqnysv-----akgwgKKQFDT 99
gi 21224347   40 RVFAPYFEAWTGespaa------------laaqsGAKHLTMAFLQtatpgsCTPYWNGdtsmpi-----aqstfGADFDT 102
gi 167461509  66 KQYVPYFDVGAYpqnpllm--------ydymtktGTQQAFLGFLVtnp-qtDELVWSGsnkpvdvlqpngrdmiFDDIRR 136
gi 117929240  40 YAQAPYVMPLDNsppdla----------tvmaatNEKAFMLAFILang-ssCSPAWDGtnpvs------sdttvANVINQ 102
gi 182440283 179 MAVAPYLYNGWGsppspt----------tvmnatGVKWFTLAFVLsn--gyCNPQWDGgrplv-------ggvdQRTINT 239
gi 29828293  271 KQAAPYLYLGWGdppsat----------svmsstGIKWYTMAFMLds--ggCNPAWDGsrplt-------ggndQSVINS 331
gi 116348      6 VSASPYLYNGWGnppspt----------evmnasGIKNFTLAFILad--gtCNPAWDGnrpl--------dgqdKATIDA 65
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  #                                       # # #                         
2DSK_A        70 LREIGGEVIIAFGGAVGPylcq----qasTPEQLAEWYIKVIDTYNATYLDFDIEAGid------adKLADALLIVQRER 139
gi 499712     93 FQQQGGRLIISFGGAAVPmwk-----pavPAPRWPRWWMPCCNAPACVPLDFDIEGSqlsqt-alnsVRNEAIKTLQAKY 166
gi 134100422  93 LREMGGDVIVASGGALGPyler----scrTADELLAAYRKTLDAVGANHLDIDVEASip------hdVVNEALARLQAER 162
gi 52842430  100 LSREGVKLTVSFGGASGTdis-----yhcDKNQLINTFSQVANQYHANVLDFDIENGtan-----ipNLLQALKLFQKEH 169
gi 21224347  103 IQANGGDVIPSFGGYTADttgteiadsctDVQQIAAAYEKVITTYDVSRLDMDIEIDaldnt-agidRRNKAIKLVQDWA 181
gi 167461509 137 LREQGKDLGIAVGGAGATmlpe----tvkSAEDAVKEYKEFLTAYGFTHLDFDIEGNltpdk-kgheNRAKVIKQLKKEL 211
gi 117929240 103 VRSLGGDVSVSIGGYAGTklgq----vcsSPQATAAAYQAVIDKYALKAIDFDLEEPeyenl-tavsNEVGAAQILQRNN 177
gi 182440283 240 VRAAGGDVIPSFGGWSGNkles----scgSAGELAAAYQKVIDAYGLKAIDIDIEAAaydsp-tvqqRTVDALKTIRANN 314
gi 29828293  332 VRSAGGDVVPSFGGWSGSklga----ncsSASALAGALQKVIDAYGLKAIDMDIENTdefenatvqdRILTALKTVKANN 407
gi 116348     66 IRGAGGDVIPSIGGYSGSklge----vcqDSQSLAGAYQKVIDAYGLKAIDVDIEATefend-asetRVLEALKIVKEAN 140
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       ##                               
2DSK_A       140 p----wVKFSFTLPSdp-giGLA-GGYGIIETMAKKgvRVDRVNPMTMDYYwt----pSNAENAIKVAENVFRQlkqiyp 209
gi 499712    167 p----dLYISFTLPTnp--tGLTsEGLNLLRGAQAAgvRTDVVNVMTMDYGasissgkKMGDLAVQAGNSLFTQlkgiyt 240
gi 134100422 163 g-----TEISYTLRVqsddtGLDpYSYQVLQSAAAHg-VDVLVNPMTMEFGss----kPWGDAVIAAAESTLGQmrqiwp 232
gi 52842430  170 p----dVLLSFTLPVmp--eGLTsVGKEIITSAATLg-LHFNVNIMAMDYGpay--sgDMGDYAISAATNLHQFlqeiyp 240
gi 21224347  182 aangrdLEISYTLPTtt--rGLAsNGVALLENAVKNgtKVDVVNLMTFDYYdnq--qhDMARDTQTATQGLHDVlarlhp 257
gi 167461509 212 qsegqpLSVSYTLALgk--sGIEpKEVAIIEPAIKEgvDISRLNFMAFDYGei---neSLVETTIKALRSTHTTlkdlyq 286
gi 117929240 178 p----gLYISITLPGta--sGTGyFGQNVLNTAKGLgfTPNNYSIMPFDGGf------NGAASQQSALQQFHTIlmntfg 245
gi 182440283 315 p----gIKLYVTFGT-----GQNgSDTSLINRAAQSglTVDSWTIMPFNFGgn---gqNMGTLSVRAAEGLKTAvkgayg 382
gi 29828293  408 p----gLRTIVTFGTtt--tGPTyWGNRLIEQAQALnaGIDVFTIMPFDFGgg----sDMYGNTVNATDGLKAKlkstfg 477
gi 116348    141 p----gLRTVVTFPTlv--nGPNdVGKRMIDKAARIgsDVDVWTQMPFNFGg-----gDMAADTITSTEGLVAHlksafg 209
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           #                                  #                         
2DSK_A       210 e----ksdeeiWKMIGLTPMIGVNDDKs--vFTLEDAQQLVDWAIQHKIGSLAFWSVDRDhpgptgevsp---lhrgtnd 280
gi 499712    241 t----ksdaelWAMVGITPMIGKNDVQte-iFTTADATQVANFAKQYNIGLLSFWALQRDrsgsgsldd-----ysltpq 310
gi 134100422 233 g----lsdaelKARLGVTPMIGRNFNGk--vFDQSHARQLVDWAAANRIGLLSFWSAGRDngrcpggpva--pdcssiaq 304
gi 52842430  241 d----kkpealWQMIEVTPMIGVNDVNte-qFTLSNAAQLKQFAQKNLLGGLSMWSFNRDkpcadkwaspv-csgnnlqs 314
gi 21224347  258 g----kssadlWHMIGVIEMIGVDDFGpaetFTLDNARTVYDWALDQGINTLSFWALQRDngscagggaa--dncsgiqq 331
gi 167461509 287 kygydktdadiWNLMGITSMTAQDDQGh--pFTLKDVQATIDFANSVGIPFLSMWSINRDsndnswv---------hpgt 355
gi 117929240 246 -----wdsataWAHEGISQMNGRSDTGe--yFYQADFQSNLDFARSVGLGRYTFWSVNRDrqcpggatsttsgtcssvvq 318
gi 182440283 383 -----ysddqaYRHTGISSMNGTTDVGe--tITVADFRTILAYAQQRHLARLTFWSVNRDrpctgggad----tcsgvgq 451
gi 29828293  478 -----wddataYSHIGISGMNGLSDQQe--nTSPATWTQIRDWANSHHIARLAFWSVNRDrpcpgggvvs---ncsgisq 547
gi 116348    210 -----yddataYAHAGISSMNGKSDTGe--tVDQAAFQKMADYAGEKGLGRLSFWSVNRDrpcdgapd------acggid 276
                        330
                 ....*....|
Feature 1                  
2DSK_A       281 pdWAFSHVFV 290
gi 499712    311 tdYQFYKAAK 320
gi 134100422 305 sdYEFTRIFG 314
gi 52842430  315 hdYEFVKYFQ 324
gi 21224347  332 ntWDFSHVFA 341
gi 167461509 356 qeGDYSKAFV 365
gi 117929240 319 ndWDFTKFDV 328
gi 182440283 452 qpWDFTRVLA 461
gi 29828293  548 ntWQFTSITA 557
gi 116348    277 qqWDFTKIVA 286

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