Conserved Protein Domain Family
TM_ABC_iron-siderophores_like

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cd06550: TM_ABC_iron-siderophores_like 
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Transmembrane subunit (TM), of Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters involved in the uptake of siderophores, heme, vitamin B12, or the divalent cations Mg2+ and Zn2+. PBP-dependent ABC transporters consist of a PBP, two TMs, and two cytoplasmic ABCs, and are mainly involved in importing solutes from the environment. The solute is captured by the PBP which delivers it to a gated translocation pathway formed by the two TMs. The TMs are bundles of alpha helices that transverse the cytoplasmic membrane multiple times. The two ABCs bind and hydrolyze ATP and drive the transport reaction. Each TM has a prominent cytoplasmic loop which contacts an ABC and represents a conserved motif. The two TMs form either a homodimer (e.g. in the case of the BtuC subunits of the Escherichia coli BtuCD vitamin B12 transporter), a heterodimer (e.g. the TroC and TroD subunits of the Treponema pallidum general transition metal transporter, TroBCD), or a pseudo-heterodimer (e.g. the FhuB protein of the E. coli ferrichrome transporter, FhuBC). FhuB contains two tandem TMs which associate to form the pseudo-heterodimer. Both FhuB TMs are found in this hierarchy.
Statistics
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PSSM-Id: 119348
View PSSM: cd06550
Aligned: 78 rows
Threshold Bit Score: 96.8572
Threshold Setting Gi: 14195338
Created: 8-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
dimer interfaceABC-ATPaseputative PBP
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interface [polypeptide binding site]
Evidence:
  • Structure:2NQ2_AB, Haemophilus influenza putative metal-chelate-type ABC transporter, interface between the two TMs (2NQ2_AB), defined at 3.5A contacts.
    View structure with Cn3D
  • Structure:1L7V_AB, Escherichia coli BtuCD transporter BtuC (2) homodimer interface, defined at 3.5A contacts.
    View structure with Cn3D
  • Structure:2QI9_AB, Escherichia coli BtuCD transporter, BtuC (2) homodimer interface,defined at 3.5A contacts.
    View structure with Cn3D
  • Comment:BtuCD (1L7V) is in an outward-facing conformation. In BtuCD (2QI9) complexed with the binding protein, BtuF, the central cavity is accessible to neither side of the membrane.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              # #    #                                                 
1L7V_B       60 TLAVLLVGAALAISGavxQALFENPLAEPGLlGVSNGAGVGLIAavllgqgq---lpnwalglCAIAGALIITLILLrfa 136
2NQ2_A       66 ILTALCVGAGLALSGvvlQGIFRNPLVNPHIiGVTSGSAFGGTLaiffgfs------lyglftSTILFGFGTLALVFlfs 139
gi 2501366   14 MVKAIWVSAIVGAVCaflSAYLMLKGWSLMGdALSHSVVPGVAGayalgf-------pyaagaFFTGMLAALAMTLVrhi 86
gi 1175231   14 MLKAMILSTAVGGICaflSSYLMLKGWSLIGdALSHSVVPGVAIayafal-------pyalgaFFAGILAALSILWIksi 86
gi 1351805   33 MQRAFVIGLIAALLAgivGSFLMLQRLTLLSdAISHSVLPGLAIafsfgi-------plifgaLLASIISVIIINWIrte 105
gi 1171961   15 LQNALITAIVIGVVAgavGCFIILRGMSLMGdAISHAVLPGVALsfilgi-------nffigaITFGLLASIIITYIkgn 87
gi 20455070  17 LQQALTAAILVGIICgviGCFIILRGMALMGdAISHAVLPGVVIaymiga-------sffigaVITGVITALAIGYVsqn 89
gi 20455062  13 LQKALITSVTVGIVSgviGSFIILRGMSLMGdAISHAVLPGVAIsymmgm-------nffigaATFGIAAALGIGFVnqk 85
gi 14195338  37 DDIQVIVFFAIAVSCsiiGTFLVLKKMAMYAnVVSHTILFGLVCaclfthqlthlsmqnltiaAISTTLLTGASIHFirn 116
gi 14195325  11 FLSSFLAVSLICMTTalwGTILLVERQPLLSeSLSHACYPGLLIgallsykvp--afsdslwvIIFFGCLASVLGCLgis 88
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                # ##  ##  ##  #  ## #             ##  #                                
1L7V_B      137 rr--hlstsRLLLAGVALGIICSALXTWAIyfsts--vdlrqLXYWXX-GGFGGVDWRQs-wLXLALIPVLLWICCQSRP 210
2NQ2_A      140 fkfnqrsllMLILIGMILSGLFSALVSLLQyisdte-eklpsIVFWLM-GSFATSNWEKllfFFVPFLLCSSILLSLSWR 217
gi 2501366   87 t-----rlrEDAIIGFIFSTFFAVGLLIVSlnpt-----svnVQSIIF-GNILGIADEDvlqVEIIILVSFVILCLIWKD 155
gi 1175231   87 s-----klkEDAVIGFIFSTFFALGLLIVSlnpt-----avnVQNIIL-GNILGIADEDiyqVAIIIGVCLVLLLLFWKD 155
gi 1351805  106 s-----rlkEDTAIGIVFASFFGLGILLISviqke---nkvdLNHFLF-GNILGITSEDlqnTSIILAIILLFFISCYRQ 176
gi 1171961   88 s-----iikSDTAIGITFSSFLALGIILISvaks-----stdLFHILF-GNILAVQDLDmwiSIGVGILVLLVISIFFKQ 156
gi 20455070  90 s-----rvkEDSAIGILFTAAFALGIVLITgmrg----tgvdLWHILF-GNVLAVSRTDlwvTLGIGLFVLLIIILFYRP 159
gi 20455062  86 s-----rikNDTAIGIVFSAFFALGIILISfaks-----stdLYHILF-GNVLAVRSSDmwmTIIIAILVISLVAIFYKE 154
gi 14195338 117 v----fkvaEEASTALVFSLLFSASLLLLVfltr-----nahVGTELViGNADALAKTDifpVFLVLLXNLGVSYCFFSS 187
gi 14195325  89 flekklamhKDSALCLVLVSFFGVGVILVSyvkdccpllynkINAYLY-GQAATLGYTEaklALIIFCLSAVVLWWWYRQ 167
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                   #                                   
1L7V_B      211 XNXLALGEISARQLGlplWFWRNVLVAATGWXVGVSVALAGAIGF-IGLVIPHILRLCGltdHRVLLPgCALAGASALLL 289
2NQ2_A      218 LNLLSLDEKEAKALGvkmAPLRWLVIFLSGSLVACQVAISGSIGW-VGLIIPHLSRMLVganHQSLLPcTMLVGATYMLL 296
gi 2501366  156 LLAVFFDESHAMSIGlspLRLKILFFTLLSACTVAALQTVGAILViAMVVTPGATAYLLtdrFGRLLIiAIAIGAITSAF 235
gi 1175231  156 LLLIFFDETQAITVGlspLFYKILFFTLLSACVVAALQTVGAILViAMVVTPGATAYLLtdkFKTLSIiAIILGAVTSFV 235
gi 1351805  177 LKCYTFDPIMAQTIGlpiNFLQSTFLILVALTIIVSMKAIGVILVlALLVTPGATGLLIgksLEYVILtSSIIGVSCSFS 256
gi 1171961  157 LLLTSFDPLLAQAMGmkvNFYHYLLMILLTLVSVTAMQSVGTILIvAMLITPAATAYLYaksLKTMILlSSALGAGASVL 236
gi 20455070 160 LLLSTFDPVMAQATGipvQMIHYLLMLLLSLVTVAALQTVGIVLVvAMLITPGATAYLLtnrLPVMLClAAMFGVISAIA 239
gi 20455062 155 FLVSSFDPVMAEAYGlnvRFLHYFLMLLLTLVTVSALQTVGIILVvAMLITPAATAYLLtnkLSKMIMlASTFGAVSAII 234
gi 14195338 188 FICVSFDTVFAFSLGirvKLIDYLMMFLLSASIVGAFKAVGVLMSlAFLLVPGLIAKLIassVQEMMGySMIFGVLSALI 267
gi 14195325 168 ISVAIFDREFAYSCGlrtRTAELVVLVFISLVIVSGVRSVGILLIsAMFVAPPLSARQLsdrLSTILIlSSIFGGICGAL 247
                       250       260       270       280
                ....*....|....*....|....*....|....*....|.
Feature 1                                 #  ##  #  ##  #
1L7V_B      290 ADIVARlala---------aaeLPIGVVTATLGAPVFIWLL 321
2NQ2_A      297 VDNVARsls----------daeIPISILTALIGAPLFGVLV 327
gi 2501366  236 GAYLSFyl-------------dGATGGVIVTLQTLVFLPAF 263
gi 1175231  236 GVYISYyl-------------dGATGGVIVTLQTLLFLVAF 263
gi 1351805  257 GMLLSYlf-------------nIPPGPTIVLITSLIFFILF 284
gi 1171961  237 GLFIGYsf-------------nVAAGSSIVLTSALIFLVSF 264
gi 20455070 240 GIYFSViy-------------dVASGASIVLVASTLFALAF 267
gi 20455062 235 GLYFSYif-------------nLASGAAMVLVATIIFFIAF 262
gi 14195338 268 APALSRsil-----------svHGIGLSTSGLAVCLLLVFY 297
gi 14195325 248 GCYFSVaftcqtvvegkpisiiLPTGPLVVFFAGVLVFLCL 288

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