Conserved Protein Domain Family
crotonase-like

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cd06558: crotonase-like 
Click on image for an interactive view with Cn3D
Crotonase/Enoyl-Coenzyme A (CoA) hydratase superfamily. This superfamily contains a diverse set of enzymes including enoyl-CoA hydratase, napthoate synthase, methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA dehydratase, and dienoyl-CoA isomerase. Many of these play important roles in fatty acid metabolism. In addition to a conserved structural core and the formation of trimers (or dimers of trimers), a common feature in this superfamily is the stabilization of an enolate anion intermediate derived from an acyl-CoA substrate. This is accomplished by two conserved backbone NH groups in active sites that form an oxyanion hole.
Statistics
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PSSM-Id: 119339
View PSSM: cd06558
Aligned: 696 rows
Threshold Bit Score: 65.6596
Threshold Setting Gi: 23397616
Created: 8-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:substrate binding site [chemical binding site]
Evidence:
  • Structure:2VSS; Pseudomonas hydroxycinnamoyl-CoA hydratase monomer with bound acetyl CoA
    View structure with Cn3D
  • Structure:1NZY, Pseudomonas 4-chlorobenzoyl CoA dehalogenase trimer with bound 4-hydroxybenzoyl-CoA, contacts at 4 A.
    View structure with Cn3D
  • Citation:PMID 8679561
  • Comment:The substrate binding site is located at the interface between two subunits in a trimer. Each trimer contains three substrate binding sites.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                # #                               #   #####             
1WDM_A         8 ITVTAles---giVELKFDLKgesVNKFNRLTLNELRQAVDaikadasvKGVIVSSgKDVFIVGADITefvenfklpda- 83
2VSS_E        11 VKVEIed----giAFVILNRPe-kRNAMSPTLNREMIDVLEtleqdpaaGVLVLTGaGEAWTAGMDLKeyfrevdagpei 85
1HNO_A        11 ISYRIeg----pfFIIHLINPd-nLNALEGEDYIYLGELLEladrnrdvYFTIIQSsGRFFSSGADFKgiakaqgddtnk 85
gi 84701644    5 IIMTQee----gvLRLRIAREt-kKNALTQEMYLTLAEAMEgvpdmpeiGAVLFEGsDEIFTAGNDLSafvtappa---- 75
gi 148554762  16 LRAHAad----gvLTVTFDRAe-kRNAITYAMYRALVRLIDhaaaadgvRALSFAAaGSIFTAGHDVSgfaqgaei---- 86
gi 67524517    8 ITLDItd----qiGVIKLNRPs-vLNSWNEAMLGEMISAFReldqhertVFTVLTGeGRFFSAGADIRqdipkapen--- 79
gi 13474694   11 LRNHT--------LIVTVSSNd-gRPVLDRRAYESLARTFHeaadndevRVVVLRGlAGCFCLGGDFSefldatkh---- 77
gi 71006160   19 VALSFahncdnrvAVITLNAPe-kLNALGWADYKCITQCLEwialqpdiLVTIFTGkGRYFSAGANVKdpsrtlpdhvak 97
gi 85086857    7 INVEYkg----rlAIITINNEk-kLNALTQMQYYDLAQRLRevathdevYITLIIGtGRYFSAGADVSisrsnvapsegl 81
gi 150866459   6 ILYEVrg----kvTIITFNIPq-kLNALNGEQYLLLAKLVEradkeedtILTLIQSsGRFFSAGANFAdkslantdaadl 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                     # ###                        ##  # 
1WDM_A        84 ---------------eliagnleanKIFSDFEdl-nVPTVAAINGIALGGGLEMCLa-aDFRVMAds--akIGLPEVKLG 144
2VSS_E        86 --------------lqekirreasqWQWKLLRmy-aKPTIAMVNGWCFGGGFSPLVa-cDLAICAde--atFGLSEINYG 147
1HNO_A        86 yps--------etskwvsnfvarnvYVTDAFIkh-sKVLICCLNGPAIGLSAALVAl-cDIVYSIndk-vyLLYPFANLG 154
gi 84701644   76 -----------------dgskppvwRLLEATArc-pVPVIAAVAGPAIGIGTTLLLh-cDLIVAApe--ayFHTPFVDLA 134
gi 148554762  87 -----------------afdekpsyLFMQRLStf-pKPVVAALNGDAVGIGATMLLh-cDLVYAVpg--ctLVFPFTKMG 145
gi 67524517   80 -----------------ataaekklFYMRKFSrdhtKILVLALNGPGVGGGAAWFTglaDIILAVsg--ayLQVPFNSLG 140
gi 13474694   78 -----------------qkliaavtDMFRTLAtf-pKPILACVDGDAVGVGCTILFh-cDMVIASne--stFRVPFVDFG 136
gi 71006160   98 adtntahgkatvadfygaraqagqgRLALALRkh-pKILIAALNGPAVGLSAAILSh-cDLVYAYdd--ffLFTPFMSLA 173
gi 85086857   82 ssqd------aihnhwlrnfvannlNITQAFYth-pKILIVGLNGPVIGLSAALVSf-aDFIYCVps--tfLLTPFSSLG 151
gi 150866459  81 fs----------heywlnrfvarntYLTELFHnh-rKILAAAVNGPVIGLSASLLAl-cDLIYVKeekdfyILTPFANLG 148
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1                                                                           
1WDM_A       145 iypgfGGTVRLPRligvDNAVEWIAsGKENRAEDALKVSAVDAVVTa------dKLGAAALDLIKRA 205
2VSS_E       148 ippgnLVSKAMADtvghRQSLYYIMtGKTFGGQKAAEMGLVNESVPl------aQLREVTIELARNL 208
1HNO_A       155 litegGTTVSLPLkfgtNTTYECLMfNKPFKYDIMCENGFISKNFNmpss-naeAFNAKVLEELREK 220
gi 84701644  135 avpegGASYLMPKrlgrQLASEFLLlSRPVPAQRAYEMGFVNAVVTe------gPVRDYGFDLAKDL 195
gi 148554762 146 lipefGSTFHLPQlighRRTMALFLgSGRCSAEQAVQWGIVNELAAp------dRLAAAAAGALADI 206
gi 67524517  141 lvpefGAAQTFAQsigvRRANDLLIfGRKCSVEELENWGLINRVFPa------qGFVDQVLAYLQGQ 201
gi 13474694  137 lvpdaATSILAPQklgyAGAFRFFClGDTLHAEDARALGLVAEIVHdgv---eeATLGRARQLAKKP 200
gi 71006160  174 lvaegLTSVTFIKkmglGRATEALLeGRRMTASDLEQSGFITRLYSkp-----aGFHAKDKLATPPI 235
gi 85086857  152 lvtegGASRGLVQrlglPKANEALImSKKITKEELVQTGFVTKCFEevgkgeteKFKGLVLKEIEER 218
gi 150866459 149 lvaegAASATLFLrlgwSKASEALLlAKPISGKDLNNLGFINKTYDgqfk-tteEFNQAVHDELVNA 214

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