Conserved Protein Domain Family
GH20_chitobiase-like

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cd06563: GH20_chitobiase-like 
Click on image for an interactive view with Cn3D
The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
Statistics
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PSSM-Id: 119333
View PSSM: cd06563
Aligned: 60 rows
Threshold Bit Score: 384.62
Threshold Setting Gi: 10835639
Created: 16-Nov-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1M01_A; Streptomyces plicatus Beta-hexosaminidase bound with product (GlcNAc), contacts at 3.5A. N-acetyl-D-glucosamine
    View structure with Cn3D
  • Comment:For 1M01_A, the most C-terminal active site Asp residue plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.
  • Structure:1C7T_A; Serratia marcescens chitobiase binds Di- N Acetyl-D-Glucosamine, contacts at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   #                                                                    
1C7T_A       311 FPYRGIFLDVARNFHKKDAVLRLLDQMAAYKLNKFHFHLSDDEGWRIEIPGLPELTevggqrchdlsettcllpqygqgp 390
1M01_A       157 YAWRSAMLDVSRHFFGVDEVKRYIDRVARYKYNKLHLHLSDDQGWRIAIDSWPRLAtyggst---------------evg 221
gi 28899260  260 FRYRGMMLDCARHFHSVEQVKRLINQLAHYKFNTFHWHLTDDEGWRIEIKSLPQLTdigawrgldetn----epqythla 335
gi 54307723  265 YSYRGMMLDCSRHFHSTKRIKHLLDQLARYKFNTFHWHLTDDEGWRIEIDAYPELTnigawrgpneei----qpqfttid 340
gi 145300008 245 FGFRGIFLDCARHFHPVANLKRLLKLMSLYKFNRFHWHLTDDEGWRLEIKAFPQLTeiaawrghdlpv----gpqlsggp 320
gi 146302535 151 FEWRGMMLDCSRQFFDKQTVKNYIDWLAAHKMNVFHWHLTDDNGWRIEIKSMPDLTlkgawrgpgevl----lpsygsgd 226
gi 118589058 271 FSWRGTHLDVSRHFRGPKDILRLLDILAWGRMNVFQWHLTDDEGWRLEIKAYPELTvsgarrgpgceq----vpqlgfaa 346
gi 86360665  161 MGWRGLHLDVARQFYGVAEVKKLLAVLAWNKLNRFHWHLSDDEAWRVEIDAYPALTeigawrghglav----ppllgssp 236
gi 99082579  264 HGWRGAHLDVSRQFYPLDQVLRYVDIMAWHKMNRFHWHLTDDEGWRLEIKAYPQLTetaahtgmdlpv----lpqlgpdm 339
gi 89069122  229 FPWRGQHLDCARHFYEPHTIRRLMDLMALLKMNRFHWHFADDEAFRLEVTCFPDVWkrtcvrgeget-----ipgvfggg 303
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          #                                             
1C7T_A       391 dvyGGFFSRQDYIDIIKYAQARQIEVIPEIDMPAHARAAVVSMearykklhaagkeqeanefrlvdqtdtsnttsvqffn 470
1M01_A       222 ggpGGYYTKAEYKEIVRYAASRHLEVVPEIDMPGHTNAALASYaelncdgvapp-------------------lytgtkv 282
gi 28899260  336 eryGGFYTQEDIKDVVAFASKRGITVIPEIDVPGHCRAAIKSLphllveaedtte------------------yrsiqhy 397
gi 54307723  341 qryGGFYSKAEVRDLIAYAHDRGITIIPEIDIPGHCRAAIKSLptllidpndhsv------------------yrsiqgy 402
gi 145300008 321 epyGGYYTQSEVRELVAYASELGITVIPEIDIPGHCHAAIKALpgllieeadrsr------------------ylsvqyf 382
gi 146302535 227 kryGGFYTQEHIKEVVAYAANRGISVMPEIEIPGHSRAVTASYpevgcaitqel--------------------ksvqge 286
gi 118589058 347 evyEGAYSQDEVREIVAHAASLNIDILPEIDVPGHSTAVLKTYprfadqaeapes------------------yhsvqgy 408
gi 86360665  237 trtGGYYTKSVIREIVAHAKSFGIEIVPEIDMPGHCYAMQQAIpelrdpaekgs-------------------yysvqgf 297
gi 99082579  340 tgqSGFYTQDEARQVVKHAAQFGIEVMPEIDVPGHCACVLGALpdlvdpeepes-------------------ywsvqgf 400
gi 89069122  304 vrsGGSYSLETARDVVAHGQALEIGVLPEIEVPAHALALARVMpelrdpsdtgq-------------------ersvqgy 364
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                 ##                                     
1C7T_A       471 rqsyLNPCLDSSQRFVDKVIGEIAQMHkeagqpIKTWHFGGDDAknirlgagytdkakpepgkgiidqsnedkpwaksqv 550
1M01_A       283 gfssLCVDKDVTYDFVDDVIGELAALTp-----GRYLHIGGDEAhs---------------------------------- 323
gi 28899260  398 ndnvINPALPGSYEFIDKVLEEVSALFp-----APYVHIGADEVpngvwskspacq------------------------ 448
gi 54307723  403 sdnvLSPALEGTYTFISTILNEICDLFp-----APYIHIGGDEVpkgvwtdsegcq------------------------ 453
gi 145300008 383 ddnvLNPALPGTYQFLEAVIDEVCELFp-----GPLVHMGGDEVptgvwtdspacq------------------------ 433
gi 146302535 287 vknvWCVGREENYVLLDSIIREVSGLFp-----FEYIHVAGDEVnranwencpkcq------------------------ 337
gi 118589058 409 pnnaLNPAMHETYEFLEKVFAEVASLFp-----FEFIHIGGDEVdvnswlespkaq------------------------ 459
gi 86360665  298 pdncINPAREQTYEIVETILAELIELFp-----FKVIHLGADEVplgawsgspaalarlrdvageava-dahakrlnvvt 371
gi 99082579  401 annaLNPAIEESYTFAETVLAEVCEIFp-----FEVVHVGGDEVaegawmqspkaq------------------------ 451
gi 89069122  365 aenvLNPARDQFWTVIEQLSEEVAALFp-----LGMLHLGCDELpegawegspava------------------------ 415
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              #                      #                  
1C7T_A       551 cqtmikegkvadmEHLPSYFGQEVSKLVKAhgidRMQAWQDglkdaesskafatsrVGVNFWDtlywg--gfdsVNDWAN 628
1M01_A       324 -----------tpKADFVAFMKRVQPIVAKyg-kTVVGWHQlagae------pvegALVQYWGldrtgdaekaeVAEAAR 385
gi 28899260  449 --almeqlgysdyKELQGHFLRHAEDKLRKlg-kRMLGWEEaqhgd-----kvskdTVIYSWLse-------eaALNCAR 513
gi 54307723  454 --qlmqqhgyqnpIELQGHLLKFAEGIIQAkg-kRMMGWEEvtkgd-----kvskdTMIFSWQne-------daGLISAQ 518
gi 145300008 434 --qlmqrqgyqdyRELQGHLLRHCQQYLASkg-kQMLGWEEilhgd-----kvsrdATIFAWTsf-------qaGLDAAA 498
gi 146302535 338 --almvkegftdsFQLQNYFFRRVQKIVDKyh-kKTDGWNEilkgg-----eidpnTLISAWQgi-------syGIESAK 402
gi 118589058 460 --rlmdekgladtMEVQAYFMGRVRGILKKln-rKLAGWDEvshggg----idpdgVLLMAWQkq-------evTKDLID 525
gi 86360665  372 nrhgaddihgsgaAILQAEFLERVQRFLASkg-cITGGWEEaahgdv----idkekSYLCSWRnv-------evSAELAE 439
gi 99082579  452 --ammretglkdtPQLQAYFLRHIQTYLAGlg-rKLGGWEEvahggg----ldpehSLLFAWTti-------ekTAELAQ 517
gi 89069122  416 --dlkareglesaDDVSGWTMAKLAGHLSErg-vRVAAWEEaargsn---ggighgALLQSWSgq-------gpGLEAAR 482
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     # #                                                                
1C7T_A       629 KGYEVVVSNPdyvYMDFPyevnpdergyywgtrfsdERKVFSFAPDnmpqnaetsvdrdgnhfnaksdkpwpgAYGLSAQ 708
1M01_A       386 NGTGLILSPAdrtYLDMKytkdtplg--lswagyveVQRSYDWDPAgylpg-----------------apadaVRGVEAP 446
gi 28899260  514 QGFDVVLQPAqttYLDMTqdyapeepg-vdwanplpLEKTYNYEPLaevpad---------------dpirkrIWGIQTA 577
gi 54307723  519 QGYDVIMQPAqytYLDLAqgfsadepg-vdwagkvpLETVYSYQPFsklste---------------dpahqrIIGTQAG 582
gi 145300008 499 AGYPVVMAPAqhlYLDLAwsqdprepg-lywagtlnLEQVYRCDPApadfh------------------andnILGVLSP 559
gi 146302535 403 KGYQTIMMPGqytYFDMAqsetergh---rwaaitdTKRAYSFEPIptddlt---------------peqqknIIGVQGA 464
gi 118589058 526 QGYDVICNPGqhyYMDMAqasgwqepg-agwagvstPQDCYTYEAStglsa-----------------gseqrLKGVQAC 587
gi 86360665  440 RGYQMVVCPGqvyYLDMAlrpdwdepg-aswagnsdAEKLYKFDPLsgwta-----------------aqkqkLLGIQAC 501
gi 99082579  518 EGYDVISTPGqayYLDMAlsdawyapg-aswagftpLDKTYAFEADngdpv------------------lqgrLKGVQAC 578
gi 89069122  483 AGYDVIMSPAqhvYLDMAhsddpddwg-aswaafvaLEDVIAWSPVppear-----------------diaerIKGVEGC 544
                        410       420       430       440       450       460
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
Feature 1         # #                                                                
1C7T_A       709 LWSEtqr-tdPQMEYMIFPRALSVAERSWHragweqdyragreykggethfvdtqalekDWLRFANIL 775
1M01_A       447 LWTEtls-dpDQLDYMAFPRLPGVAELGWSpas------------------------thDWDTYKVRL 489
gi 28899260  578 LWCEiin-nqSRMDYMVFPRLTAMAEACWTdkq------------------------hrDWTDYLSRL 620
gi 54307723  583 LWCElin-nqSRFEYMLFPRLLAIAEVCWSqpk------------------------qrNWDDFKARL 625
gi 145300008 560 LWSElvt-srDRLDYMLFPRMLATAEVAWSdat------------------------rkAWDHFAARL 602
gi 146302535 465 LWSEyldrpaRIMEYQSYPRISALSEIGWSkke------------------------dkNWEDFYGRL 508
gi 118589058 588 IWCEhmt-dnVIFNHMVFPRLYAVAEAGWTkpt------------------------nkDWQRFAAQA 630
gi 86360665  502 IWSEpmt-drAVFDRLVFPRISGLAETGWTkps------------------------akSWDRFKALA 544
gi 99082579  579 VWSEhlt-tmARRNHMIFPRLSAIAEAGWSaae------------------------nkAYDRFKSLA 621
gi 89069122  545 FWSEftt-hdREMEAMVAPRILGVAAKGWDitd------------------------rlTGAQLRGLA 587

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