Conserved Protein Domain Family
GH20_GcnA-like

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cd06565: GH20_GcnA-like 
Click on image for an interactive view with Cn3D
Glycosyl hydrolase family 20 (GH20) catalytic domain of N-acetyl-beta-D-glucosaminidase (GcnA, also known as BhsA) and related proteins. GcnA is an exoglucosidase which cleaves N-acetyl-beta-D-galactosamine (NAG) and N-acetyl-beta-D-galactosamine residues from 4-methylumbelliferylated (4MU) substrates, as well as cleaving NAG from chito-oligosaccharides (i.e. NAG polymers). In contrast, sulfated forms of the substrate are unable to be cleaved and act instead as mild competitive inhibitors. Additionally, the enzyme is known to be poisoned by several first-row transition metals as well as by mercury. GcnA forms a homodimer with subunits comprised of three domains, an N-terminal zincin-like domain, this central catalytic GH20 domain, and a C-terminal alpha helical domain. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
Statistics
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PSSM-Id: 119335
View PSSM: cd06565
Aligned: 44 rows
Threshold Bit Score: 267.15
Threshold Setting Gi: 182412524
Created: 15-Nov-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
putative active
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #                                                                      
2EPL_X        86 DLAYMADCSRnAVLNLSSAKKMIEVLALMGYSTFELYMEDTYEIENQPYFgyfrgRYTVAELQEIEDYAADFDMSFVPCI 165
gi 78099325   22 FRGFMLDVSCdRVPTRSTLDFLTRVMVRCGLNHLELYGEAGFAYRGEEEVwadrsPVTAEDMRWLDGLCRGRGIELGANQ 101
gi 23464684  184 VRAYSLDVTRgRVPTMAFLTWFIDQLALYKYNQFQLYVEHAFAFGELSEAwrgtdPLTADDIMFLDEYCAHHGIELVPSL 263
gi 119720203 101 FRGFHLDVARgGVATVEELKRLLRWLFLLKYNYLAVYVEDLFPWDRYPDIgarrgRYTGKEWREVVEYGGRLGVEVFPSL 180
gi 20808334   99 FRGYHLDIARgGVPNLDTFKKILRWLFLLKYNYFGIYFEDLFPWQKYPQIgklrgRLSREELEEIISYGENLGIEVFPSL 178
gi 146297532  97 FRGFHLDIARgGVPSVSTFKNILRWLFLLKINYFAIYFEDLFPWEKHPKIgagrgRLTKEELKEIIEYGKNLGIEVFPSL 176
gi 51891442  160 RRGVMLDISRgKVPTMESLYRFVDLLADLKINEFQLYTEHTFAYREHREVwqaysPMTGEEIMLLDRYCRERFIDLVPNQ 239
gi 18144641  131 YRGFYHDVTRgMVPTLDTLKSLVDKAAFYKINQLQLYIEHTFAFKGMSEVwmdkdPLTAEEILILDKYCKERHVELVPSL 210
gi 189467305 155 YRAVHFDVKH-HLDRTEYYYRAIDRLARYKINAVIWELEDKLRYTRRPEVaa-gnAISKQEMQAICRYAQERNVEISPLV 232
gi 126648857 144 YRAVHLDVKH-HLEKKEYYYELMDRLAELKINAIIIEIEDKLGYELQPVVas-qdAYSIDEWKAISDYAMARNIRISPLV 221
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                                                                     ##    
2EPL_X       166 QTLAHLSAFVKWgikEVQELRDve---------------diLLIGEEKVYDLIEGMFQTMAHLHt--RKINIGMDEAHLV 228
gi 78099325  102 NCLGHMEPWLETe--SHHHRCEnpdgvdlp---wgvharasTLAPVPENLDFVQRLLGELTETVs-sKFLNVGLDEPWEL 175
gi 23464684  264 ATFGHMYMNLRTr--EHRGLGEfpedadrpfsfiermehhtLNAANPKSHDFASRLIEEYAPLFr-sRSFNIGGDETFDL 340
gi 119720203 181 ELAGHMENILSLp--GYRRFSEwhrp-----------eegcLDVGDPEARRFAEELLEEALEKTk-sRYIHIGGDETWAM 246
gi 20808334  179 ELCGHMENILSLp--EFTRFSEwhrp-----------qegtINVFDEEAREFTYDLLREVVEFFp-sEHIHIGGDETWAL 244
gi 146297532 177 ELTGHMENILSIp--EYSKYSEwylp-----------regcLDLSSEEAKRFAYELLEEVLEFFp-sKYVHIGGDETWAL 242
gi 51891442  240 NSFGHMTPWLIHs--RYKHMAEapdgfelpw-ggrrnepfsLSPAEPSVISFLAGLYDELLPHFt-sQYFNVGCDETWDV 315
gi 18144641  211 STFGHLYEALRSk--SFRELCEleigdeeys-fvdrmahhtLDVTNPKSLDFVESMLLEFIPLFs-sDKFNICCDETFDL 286
gi 189467305 233 QGLGHAGFILKHh-wELRENPSsd---------------weFCPTNPKTYEFQFDLYRDALEAMpygRFLHVGGDETMAI 296
gi 126648857 222 QGLGHASFILKHe--EYAALRDdpas------------dwaFNPLDDKTYDVQFDLYADALKAFphaDYLHVGGDEVHTT 287
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              #                                         
2EPL_X       229 GLGrylikhgfqnrSLLMCQHLERVLDIADKYGFNCQMWSDMffklmsadgqy-----------drdveipeetrVYLDR 297
gi 78099325  176 GTGvsadrcrdegkGVVYAKWARDVVTPWLDRGWRVAMWADIvns----------------------------hpEALDI 227
gi 23464684  341 GRGrsvqdspgasrDELYADFVKDLCSTLAHRGLQPMLWADIale----------------------------npHTMDL 392
gi 119720203 247 GRGrsldrtlrfewPRLYAEHHSRLLRLARERGKTPLIWGDMlagmylre-----------------terdlwrpILENP 309
gi 20808334  245 GRGksldktntfkgPELFELHHRNLINIVRDKGKTPIVWGDMltgmylrp-----------------derekwkvVLESE 307
gi 146297532 243 GRGksleknwifegPKLYEEHHKNMIDMVEKYRKIPIMWADMltgmflrp-----------------derkvwenLLQSD 305
gi 51891442  316 GQGrsaravaergeHEVYLEQLLKIHRLVAERRRTMQFWGDIiik----------------------------ypEAVAR 367
gi 18144641  287 GKGksrekaeklgvGKIYTEFLNKVYNIVKSFNKNVMFWGDIivg----------------------------ypELLSD 338
gi 189467305 297 GIDercka-tdksaFELQMGWLRKVCDFAKSNGRIPIFWDDMplkygglwdmahgd-----ndtddlhwdtqkldSVIDL 370
gi 126648857 288 GRGsgm------spLELQLTWLNRVSKYAEDNGLTPIFWDDMplknaglygsiydsklskaqadsiwaaneyklnEYVDM 361
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #                                                                      
2EPL_X       298 LKERVTLVYWDYYQDseek---------------ynrnFQNHHKISQDIAFAGGAWKWIGFtPHNHFSRLVAIEAnkacr 362
gi 78099325  228 IPDEVLLIPWTYESPameakfagtegyeirsdagfastARLVANAGRPFVNAPGTGAWNTFtGRLSNAVGNIVDAvitdh 307
gi 23464684  393 LPGDITMLNWMYEPDide------------------skIQTIASQGRRQFVCPAVRAWSRFfPDYDGAWLNTYRMavagl 454
gi 119720203 310 AWREAVVANWDYSPGtvey---------------fkqkIRLFKERGYEQIVCPGLWNWDRYyPDFDAALANVKSFlqaar 374
gi 20808334  308 IWDDVIIANWDYTNLsekh---------------frekINLFGKRKEKQIVCPGFSNWGRFyPSFDIAITNITNFlkpak 372
gi 146297532 306 IWQRTILANWDYAAMpkehf--------------inriESLGKNYQSNQIVCPGFSNWNRFyPDFEAAIENIKNFidaak 371
gi 51891442  368 LPKDVIALEWGYEADhpf-----------------dehCAVYAHAGVPFYVCPGTSTWMSLvGRTENALQNMRSAaesgl 430
gi 18144641  339 IPEDLTCLTWNYHPQand------------------vaTKIIAEHNKVQYVCPGVGGWNMMmNLIEGSFSNIRRMvnhgi 400
gi 189467305 371 FPKDCVYMRWNYRDAtrpgh--------------qrilKWYHDKGLRVMAATAASSGNSPVlPREDTKAGYIKGFsrlva 436
gi 126648857 362 FPKNCVYMRWNYSLPespgn--------------nlaiQWFTKNGFNVMGATAGQTRWNLM-PLENSNTQNIRDFanisi 426
                        330       340       350
                 ....*....|....*....|....*....|....*...
Feature 1                   # #                        
2EPL_X       363 knqVKEVIVTGWGDNGGETSq-fSVLPALQIWAELAYR 399
gi 78099325  308 slgGKGVLLTGWGDSGHHAGlvfSLVPLVVTGVAMADP 345
gi 23464684  455 kygAEGMVVTDWGDYGHVNDp-rLSVPGLCYGAQNAWN 491
gi 119720203 375 eegVKGFMVTAWGDDGEECLy-sFLYPLILASMEYAEG 411
gi 20808334  373 eegVKGFLVTSWGDDGAEALy-sFLDPLILANMEIAEG 409
gi 146297532 372 ekgIQGFLVTSWGDDGQECLf-sFLYPLLVATIEFAEG 408
gi 51891442  431 khgAQGFLNTIWGDLGHQDYepvAYLPVAFGAAVSWAL 468
gi 18144641  401 kygAIGVLNTNWGDYGNINLl-aNSIPSMIYGAGISWN 437
gi 189467305 437 qnqLEGILATSWDDGSPHLE---TVWRGYIAQGEFGWN 471
gi 126648857 427 nnkADGLLLTLWDDDSPLFE---LYQRGIATFSEYTWS 461

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