Conserved Protein Domain Family
GH20_SpHex_like

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cd06568: GH20_SpHex_like 
Click on image for an interactive view with Cn3D
A subgroup of the Glycosyl hydrolase family 20 (GH20) catalytic domain found in proteins similar to the N-acetylhexosaminidase from Streptomyces plicatus (SpHex). SpHex catalyzes the hydrolysis of N-acetyl-beta-hexosaminides. An Asp residue within the active site plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. Proteins belonging to this subgroup lack the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases.
Statistics
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PSSM-Id: 119336
View PSSM: cd06568
Aligned: 14 rows
Threshold Bit Score: 404.793
Threshold Setting Gi: 117164684
Created: 22-Aug-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1M01_A; Streptomyces plicatus Beta-hexosaminidase bound with product (GlcNAc), contacts at 3.5A. N-acetyl-D-glucosamine
    View structure with Cn3D
  • Comment:For 1M01_A, the most C-terminal active site Asp residue plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #                                                                    
1M01_A        157 YAWRSAMLDVSRHFFGVDEVKRYIDRVARYKYNKLHLHLSDDQGWRIAIDSWPRLAtyggst------------------ 218
gi 187934322  190 YNYRGLMIDVVRHFFTIDEVKRQIDYAAQYKINRVHLHLSDDQGWRLEIKKYPDLTiiggst------------------ 251
gi 169350875  189 YEYRGSHLDVTRHFFSVEDVKRYIDNMAQYKMNKLHLHLSDDQGWRLEIKGEMYGEslsklntiga-----------qts 257
gi 86142069   165 FEYRGAMLDVARHFFTVEDVKKYIDLLAYYKYNVLHLHLTDDQGWRIEIKSWPKLTeigget------------------ 226
gi 86132308   184 FAYRGMMLDVARHFFTVNQVKRVIDQMASYKLNKLHLHLTDDQGWRIEIKSWPKLTeiggss------------------ 245
gi 88855794   148 FSYRGAMLDVARHFFDVATVKRHLDRMSLLKLNVLHLHLTDDQGWRIHIDSWPNLTalgatt------------------ 209
gi 117927283  203 FAYRGAMLDVARHFFPVADVERYIDELALYKVNVLHLHLSDDQGWRIAIDSWPKLApvggkt------------------ 264
gi 119962520  138 FAYRGLMLDVARNFFTVQEVKEQIDVMTQFKFNALHLHLTDDQAWRIEIHEPAKNPsglpyakltevggq---gavevst 214
gi 6911555    487 FDKRGMMLDVAREFKNPDEVKAIIDSLASYKISTLHMHLADDQGWRIEITNEGKVAgddidynqlteisgkggmtqfnrt 566
gi 150006724  178 FAWRGLSFDVSRCFFDPEEVKQVIDMIALYKMNVLHMHLSDNQGWRIEIKKYPELAeiggql------------------ 239
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               #                                         
1M01_A        219 -evgggpGGYYTKAEYKEIVRYAASRHLEVVPEIDMPGHTNAALASYAELncdgvapply---------tgtkvgfssLC 288
gi 187934322  252 -evgggkGGYYTQEEFKDLVKYAAERYVEIIPELDMPGHSNAALASYGFLnkdgekkply---------tgtevgfssLM 321
gi 169350875  258 tsingikAGQYTQEDYKEIVAYAADRYVEIIPEFDMPGHSWAALVSLNFLnssedgkphsgnydntkpyegidvgfstFE 337
gi 86142069   227 -evgnepGGFYTQEDYKAIVAYAAQHHMTIVPEVDMPGHTNAASVAYPFLngngktpkiy---------rgtqvgfstFD 296
gi 86132308   246 -svrdesPGFYTQEDYKEIVAYAQSKYITVIPEIDMPGHTNAALASYPELncngkatkly---------rgmrvgfstLC 315
gi 88855794   210 -avgggnGGFYSKADYTEIVEFAASRYITVVPEIDVPGHTNAALSAYPELncddvapapy---------tgievgfssLC 279
gi 117927283  265 -evgggpGGYYTQADYRAIVAYAQAHFITVVPEIETPGHVNAALVAYPQLacsgkpirpy---------tgtgvgfssLC 334
gi 119962520  215 ampprgnSGFYTQQDFKDIQAYAATKNVLVIPEIDLPGHVNAALAAIPQLnpdgqakpms---------ttsevgwstLT 285
gi 6911555    567 ymdllgnTGFYTQAEYKDLVAYAADRHIEIIPEIDVPGHTSAILHAIPQLntagtkpnvdewgv-vpedgtgnvgtstLD 645
gi 150006724  240 -pnngrkGGYYTQEEFKDLVNYAKERFITIIPEVDIPGHTAAIFAAYPDFknavkfktnv---------nipgqafnaLD 309
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       ##                                                
1M01_A        289 VDKDVTYDFVDDVIGELAALTpGRYLHIGGDEAhstpKADFVAFMKRVQPIVAKygKTVVGWHQlagae--pvegALVQY 366
gi 187934322  322 THNEETYEFIDNVIKEVSEISpSKYIHIGGDEAdstkKSDYDYFVGRVSKIIEKygKTPIGWDPidisp-einssVILQN 400
gi 169350875  338 CRNEKTYEFIDEVFRQVAAISpSKYIHIGGDEAhstsSEDYAYFMNRVTEIAKKygKTPIGWQNydgva-edkegTVTQF 416
gi 86142069   297 TRKDTVYSFIDDVVREISAITpGPYFHIGGDEShvtqKDDYIYFVNKVEQIVQKydKKMIGWDEvatad--idttSIAQF 374
gi 86132308   316 VDKEITYKFIDDVIREVTEITpGEYIHLGGDEShvtsKKDYNIFLNKVFPIVKKygKSVVGWEEiqsan--idstYVIQH 393
gi 88855794   280 IDKDVTYQFLDDVFREVSELTpGPYLHLGGDESlatsDEDYLEFIRRATAIAAShgKALIGWHEmgrsr-dlpagTVGQY 358
gi 117927283  335 ISNPTVYQFVDDVVGELAALTpGPYIHLGGDEAmstpPSEYAAFVQKAQAIVEAhgKTLMGWAEiakgs--ldasAVAEY 412
gi 119962520  286 EDLPATYEFVREVLGQLAAITvGPYLHIGGDEAhvtgHDDYVSMVQRFVRIGAEtgKSVVGWNEfaave--lpqgSVIQY 363
gi 6911555    646 VAAPQTWTFLEHVFGQIAEMTtSEYIHIGGDEShvtgHDNYVEFITKAVKLIHDldKKPIGWNEvaiggleaggrHSVLD 725
gi 150006724  310 VDDPKAMQFTEDVIAELAALApGNYIHIGGDEAiglpHDKFVRFINKTREIVLKngKKMVGWQEtarad--isegDVIQH 387
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #                                                         # #                   
1M01_A        367 WGldrtgd--------------------------aekaeVAEAARNGTGLILSPAdrtYLDMKYtkdtplgl-------- 412
gi 187934322  401 WKd-----------------------------------sNEAARKKEMKMIISIAqkaYLDMKYnestpygl-------- 437
gi 169350875  417 WSt------------------------------------GNAKLKDGIKYVVSPAdyaYMDMKYdascelgl-------- 452
gi 86142069   375 WQsq---------------------------------knAELAVAKNLKVIMSPAkkaYLDMKYdlkseygl-------- 413
gi 86132308   394 WQke---------------------------------atAQKGIDKGAKVILSPAkkmYLDMKYtklspigl-------- 432
gi 88855794   359 WSyvaprd--------------------------dadkrVASFIEQGGQMIMSPAdaiYLDMKYasdeelgl-------- 404
gi 117927283  413 WNfrdg------------------------------masARQALARGMRLVAAPAdhaYLDQKYtatsrlgl-------- 454
gi 119962520  364 WHgdf-------------------------------eptVRQAEQNGSRVIMSPAantYLDQKYasdspvgle------- 405
gi 6911555    726 RRhr---------------------------------rhAEGHQDKGAKLMVSNGstaYLDMKYnaktpigl-------- 764
gi 150006724  388 WIylkqknedsskkkdnipaeykeimalfaefmkeapkdPGLGISKKAKVILSPSgyvYLDHKYlepsadstqnaeqerl 467
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                  # #                                    
1M01_A        413 --swagyveVQRSYDWDPAgylpg----apadaVRGVEAPLWTETLsdpDQLDYMAFPRLPGVAELGWSpasthDWDTYK 486
gi 187934322  438 --twagyipVETAYKWDPTdyap-------kelVLGIEAPLWTETIsdaKAMDYMIYPRLLGYAEIGWTpkenrDWSQYK 508
gi 169350875  453 --qwagynsIEDTYSWDPTnygd-------kenIVGIEACLWSETLannDHLDYMAYPKILSHAEIGWTpkelrSWDTYK 523
gi 86142069   414 --nwagyvpVNTAYVWKPEayeni-----prehILGIEAPLWSETIstiSELEYLAFPRAIGYAELNWSqpeqrDWENYK 486
gi 86132308   433 --twagmveVDSAYIWQPSsifkd----idtsqLLGLESPLWAETIqtsDDIEYLAFPRVIGHAELGWSnpanyNWDNYR 506
gi 88855794   405 -ewadgpttLHDAYTWDPAaitig----vtdehILGIEAPLWTETIdnvRDLEYMVFPRIIAAAEIAWSprsahDWADFT 479
gi 117927283  455 --twagpvsVAEAYAWDPTmiap-------dgdVLGVEAPLWSETIrtmADIEYLAWPRMAGIAEIGWTpqserSWQEYR 525
gi 119962520  406 -wveggpftWSEYYNWDPAqggl------qdqqILGVEGPLWTETVrnnQQAQWLLYPRAVSLAEVAWSgqevrNPGDFR 478
gi 6911555    765 --twagmgdFPKYYDWNPAavvkdgttnlpdsaILGVEAPQWSETIrggKQTEFMVFPRVISFAEVGWTpqakrNVSDFK 842
gi 150006724  468 gmaayekqtIQEMYDWDPMtfnptv--enpqkdVAGIEAAIWCETItnfRDLQFLLMPRLAGVAEKGWSkventHWDEYK 545

                  ...
Feature 1            
1M01_A        487 VRL 489
gi 187934322  509 NRL 511
gi 169350875  524 PRL 526
gi 86142069   487 VRL 489
gi 86132308   507 VRL 509
gi 88855794   480 RRL 482
gi 117927283  526 LRL 528
gi 119962520  479 RRL 481
gi 6911555    843 VRM 845
gi 150006724  546 VRL 548

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