Conserved Protein Domain Family
GH20_Sm-chitobiase-like

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cd06569: GH20_Sm-chitobiase-like 
Click on image for an interactive view with Cn3D
The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
Statistics
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PSSM-Id: 119337
View PSSM: cd06569
Aligned: 20 rows
Threshold Bit Score: 676.703
Threshold Setting Gi: 5881581
Created: 22-Aug-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1C7T_A; Serratia marcescens chitobiase binds Di- N Acetyl-D-Glucosamine, contacts at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        #                                                                
1C7T_A        307 DAPRFPYRGIFLDVARNFHKKDAVLRLLDQMAAYKLNKFHFHLSDDEGWRIEIPGLPELTEVGGQRCHDLsettCLLPQY 386
gi 5881581    352 DAPRFEYRGMMLDVSRNFQSKETVFKLLDLMAFYKLNKFEINLANDEGWRLEIPGIPELTEFGAKRGYDLsestMLHTFM 431
gi 7023983    300 DAPRFAYRGVHLDVGRNFSSKESVLRLLDCMALYKLNQFHFHLTDDEGWRVEIPSLPELTEIGSGRGFTItetdNLVPCF 379
gi 11177034   336 DSPRFSWRGMHYDNARNYHGKDALFKLIEQMARYKLNKFHWHFSDDEGWRLEIPGLPELTEVGAFRCFDLqereCLLTQL 415
gi 83815976   341 DAPRFDHRGLHLDVARNMQSVAAVKRLLDIMAFYKLNTFHFHLTDDEGWRLAVEGLPELTRVGGRRGHTLdeqaHLMPSY 420
gi 115373394  358 DAPGFVYRGMHLDVGRHFQSKETVKKLLDVISHFKINKFNIHLTDDEGWRLETPGIPELTSYGARRGFDLaeaeMLHAGL 437
gi 149280173  310 DAPRFGHRAFLLDIARNFQSKDEVYKIIDLMALYKMNVLHLHLNDDEGWRIEIDGLPELTSIGSRRGHSIan-dMLPPSY 388
gi 83647475   348 DAPRFSYRGMHLDVARHFSQPESVKKLIDVMALYKLNKLHLHLSDDEGWRLEIPGLPELTSVGGKRGHSEneldNLVPSF 427
gi 156396761  330 DAPRFEYRGMEIDLGRNFMPKSEILKLIDATSMYKLNKLHLHLTDDEGWRLEIPGLPELTTLGSKRCHDPkehdCIDSQL 409
gi 167762009  287 DYPDFSYRGHMQDVARNFIELSDLKKLIDILSSYKLNVFRFHFSDDEGWRLQIPGLEELTEIASHRGHTYneseCLYPAY 366
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                    #    
1C7T_A        387 Gqgp-------------------------------------dvyGGFFSRQDYIDIIKYAQARQIEVIPEIDMPAHARAA 429
gi 5881581    432 GaangfapgdgienkptdetaanlgtapsyqgfeiaqqnflgkgWGYYTVNDFKEILQYAADRHIDVIIEYDFPAHARAA 511
gi 7023983    380 Gsgaev---------------------------------egshgTGFYTRAEFVEILKFATARHIEVVPEFDVPGHARSA 426
gi 11177034   416 Gtgpfk----------------------------------tgsgNGYLSREDFVELLKFATARHIEIIPEIESPGHARAA 461
gi 83815976   421 Gsgpsps-------------------------------peasrgSGWYSRADYLDILRYAKARHITVMPEIDVPGHARAA 469
gi 115373394  438 GsgsdlqdgdlirlkpatpqkanaetppayqgfetatlnfvgkgSGYYTTKDFEEILKYATERHIEVIPEIDVPGHARAA 517
gi 149280173  389 Gsgpgt---------------------------------dnpfgTGYFSRKEYIALLKYAAARYVDIIPEIETPGHARAA 435
gi 83647475   428 Gsgpfa---------------------------------dsnngSGYFSRRQYIELLRYAAERHIEVVPEFDLPGHARAA 474
gi 156396761  410 Gsgpgt----------------------------------gnsgTGYYTVSEYRDILRYARDRHVEVIPEFDMPGHGHAA 455
gi 167762009  367 Ngdfdyq--------------------------------sktsgNGFYSRDEFIDLLRYAADRHVAIIPEIESPGHARAA 414
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                             ##                                          
1C7T_A        430 VVSMEARYKKLhaagkeqeaneFRLVDQTDTSNTTSVQFFnrqsyLNPCLDSSQRFVDKVIGEIAQMHkea-gqpIKTWH 508
gi 5881581    512 IKAMEHRYNKYkdsdp-veanrFRLIDPLDQSQYYTPQFYtd-nfVNPALESTFTFLNHVVRETRAIYdavpdatLTRLH 589
gi 7023983    427 IKAMNVRYDRLvkagqqaeaekYLLVDFDDKSKYESVQLWtd-nvICIAREGAYNFIETVIHDVKDMYaea-gapWTTMH 504
gi 11177034   462 IKSMEARYHKLmeagkaqeakaYLLNDIDDTSKYLTVQLYtd-nsINVCLDSSYAFMEKVVYELQEMYrda-gtmLTTVH 539
gi 83815976   470 IQAMEARTRRLraagdtaaaraYRLRDPADTSDYESVQGWdd-nvMNVCRPSTYRFLSTVVDELRGLHeaa-gapLPAVH 547
gi 115373394  518 VKAMEYRYRKLkdldp-aqaaaYRLIDPNDTSKHKSVQGYtd-nfINPCLDTSYAFLTKVAEEIKARYaaa-gapLKTIH 594
gi 149280173  436 IKSMDARYDYYmkkgkkqeaeeFLLRDRADSSVYRSVQGWnd-nvINPALPSTYQFIRKVTGELVRMYaea-gapLKTIH 513
gi 83647475   475 IKSMEARAKHFaeagkpyqgrnFLLSDPEDKSEYKSVQGWkd-nvINPCMPTTYFFIDKVVGEVSRMYrqa-glrMPAFH 552
gi 156396761  456 IKAMQSRYRKYlamgdkykanqYILSDPLDKSEYLSVQFFtd-naINPCLESTYEFIEHIVFQVRRMHfdv--qpLKTFH 532
gi 167762009  415 IVAMKARYKKYqdtdp-ikaheYLLHDIHDTSQYVSAQGYsd-nvMNVAMPSTYRFMKKVIQEIQLMYkea-gvpLRSIH 491
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            ##                                                                           
1C7T_A        509 FGGDDAknirlgagytdkakpepgkgiidqsnedkpwaksqvcqtmikegkvadmEHLPSYFGQEVSKLVKAhgidRMQA 588
gi 5881581    590 GGGDELphlgpnewwaqsp-----------------------avdqnpatagktdAELFDYFFLRWKQIVNQhg-fDMAT 645
gi 7023983    505 TGGDEVphgawegspicq--------------------------kfmkdnalesiQELQDHFLARYRDILKKyg-lTFGG 557
gi 11177034   540 FGGDEVgkgswtespacnd------------------------lfavadngvagpNDLKPYFTQKVAKLLAKrg-iTPAA 594
gi 83815976   548 VGGDEVpegawagspicd-------------------------dyiartegvdgaDDLFGHFLGRFQDTLATrg-iAMAG 601
gi 115373394  595 AGGDELpalnpnvwwqgsp-----------------------lckanpatkdmddHQLFNHFFTRWNQIIKAtg-aEMTG 650
gi 149280173  514 MGGDEVpegvwakspavl-------------------------qllekepsigsvYGMWKYYFSKVNDILKTqh-lYLSG 567
gi 83647475   553 VGADEVpagvwkkspactr------------------------mfgseelsadevETLNRFFNATVTGIVAAhg-tKIAG 607
gi 156396761  533 FGGDEVahgawtkspacaal---------------------aqklglnftspsivKDLKEYFVRRVSKITSKyc-lDLGS 590
gi 167762009  492 IGGDEVaegawkgspick--------------------------nfmlehnmtdvQELSDYFIMRIVDFLKEqn-iPFSG 544
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #                                   #                                           
1C7T_A        589 WQDglkdaess-------------kafatsrVGVNFWDtlywg------------------gfdsVNDWANKGYEVVVSN 637
gi 5881581    646 WGDvlshngtgn----------vsygeiypvFWNNVWGwgn---------------------ehqAYKFANQGHKVVLAH 694
gi 7023983    558 WEEialkketvegvthhgpnpkfvdanfrpyIWNNVWGwgq---------------------edfAYQLANAGYKVVLCN 616
gi 11177034   595 WEDglmynttttf---------krdefpnpqFLVNTWDniwew-----------------gvadrAHRFANNNYQVILSH 648
gi 83815976   602 WEEvgleeadhrsattt-pnealvdddvqpyVWSNIWGggt---------------------edrAYRLANAGYDVVMAQ 659
gi 115373394  651 WDDiihnglt--------------lpgfipmPWSNVWGwgr---------------------eddAYKYANQGYQVILSH 695
gi 149280173  568 WEEiglkktlvngkg----kwitdptvsgrgYTTDVWNniigt-----------------gaedlAYKLANGGFKVVLSN 626
gi 83647475   608 WEElafmhedggkvv----nphfvggimipyVWNNVWGwgt---------------------ednAYKLANAGYPVVLAN 662
gi 156396761  591 WEDgvlgvkdkpydr-----gylpnrnvyayAWDNVWEwghgsrayklanagykashffragidkTFSSINVAAQVVMTQ 665
gi 167762009  545 WQEiaaghdkdf-----------drylsenaLGISCWKasphs-----------------hsdelVYQFANKGYPVILCN 596
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             # ##          # #                                                           
1C7T_A        638 PdyVYMDFPYEvnPDERGYYWgtrfsdERKVFSFAPDnmpqnaetsvdrdg--------nhfnaksdkpwpgAYGLSAQL 709
gi 5881581    695 AtnLYLDLAYNkhPDEIGYHWagy-tdTKKAFEYRPFniyanaerdklgnpvp----wdpkwvllseagkqnVQGIQAQL 769
gi 7023983    617 VtnLYFDLAYEkdPKEPGYYWggf-inTRKAYEFVPLdiyttatvnlfghdlg---dslkdkarltpegtknVIGIQGEL 692
gi 11177034   649 GthLYFDHPYEahPEERGYYWatrytdTKKAFSYMPDniyanadftrnrepivn-lealvgrelpalkrpqnILGMQGQI 727
gi 83815976   660 AtnFYFDMAYSkhPREDGYYWagf-vdTKAPFAFVPFdlyksadrtrmghpidp-dtafadqvrladtarnnIRGLQGQL 737
gi 115373394  696 StnLYMDLAYNkdPDEPGYYWanf-vdEKKTFEYRPFdiyanathdrmgnaikp--edlaskvrltaegkknILGMHGLL 772
gi 149280173  627 VtnNYLDMAYNnsFYEHGMNWagf-vnVDKPFYFIPYnyylttteneygepvaq--sffrnkdqlkaesknnIIGLQSAL 703
gi 83647475   663 AtnLYFDLAYQkaPEEPGYYWagf-vdTRRVYEFTPMdvfkaawvdrmgnpltd--dmwndrtrlaadkqdmILGVQGEL 739
gi 156396761  666 AthLYFDHPYEpdPEERGYYWapryidTRKTFGFMPDnlyanadftrmgdpivdlcqtmykgqcvplkepnnIAGMAGAL 745
gi 167762009  597 AsnFYFDLAYDkhPNEPGHNWngy-vdESKSFSLLPYsiyrsirnrqvek----------ektrlteqgrknIKGVESAL 665
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         # #                                                                             
1C7T_A        710 WSEtqrtdPQMEYMIFPRALSVAERSWHragweqdyragreyk-------------------------ggethfvdtqal 764
gi 5881581    770 FGEnqkspEILDYMTFPKLLGAAERAWVqdmpaq------------------------------------------gaat 807
gi 7023983    693 WGEnlrdaGRLEYLAMPRIVALAERAWAkdpgwtqiad----------------------------------ktardakm 738
gi 11177034   728 WSEtirseDQVLRLIFPRLLSLAERAWHkadwegrr--------------------------------------inqkel 769
gi 83815976   738 WGEtlrkpDRMEYMAVPRLLSLAERAWAprpgwatldd----------------------------------sgalrarr 783
gi 115373394  773 WGEnlktpEVVEYLAFPKVLGVAERAWNpelpev-------------------------------------------sam 809
gi 149280173  704 WSEtlvaeGLLEYMVLPKLLGSAERSWAkdpawsgtqg----------------------------------aifssqey 749
gi 83647475   740 WSEnvkteSDLFYLALPKLLGLAERAWSpqpdwanead----------------------------------qaqrlsgl 785
gi 156396761  746 WSEtvrtpSQLQSMIYPRLLALAERAWHkacweiesdv-----------------------------------tersrlq 790
gi 167762009  666 WSEtirnfEQVEYYLFPKIMGLSERGWHaspqwenitgiheqytyekdlsfyyqrisqkeipywekmevnyrlpfpglyi 745
                         570
                  ....*....|.
Feature 1                    
1C7T_A        765 ekDWLRFANIL 775
gi 5881581    808 teAWHTFTNTL 818
gi 7023983    739 daDWNQFANRL 749
gi 11177034   770 nkDFSTFAAAL 780
gi 83815976   784 aeAWTAFANRL 794
gi 115373394  810 paLWGQFTNSL 820
gi 149280173  750 kqAWSTFTSTI 760
gi 83647475   786 nqAWSGFAARV 796
gi 156396761  791 vmDWEMFANAL 801
gi 167762009  746 dnDGILFANSS 756

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