Conserved Protein Domain Family
Histidinol_dh

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cd06572: Histidinol_dh 
Click on image for an interactive view with Cn3D
Histidinol dehydrogenase, HisD, E.C 1.1.1.23. Histidinol dehydrogenase catalyzes the last two steps in the L-histidine biosynthesis pathway, which is conserved in bacteria, archaea, fungi, and plants. These last two steps are (i) the NAD-dependent oxidation of L-histidinol to L-histidinaldehyde, and (ii) the NAD-dependent oxidation of L-histidinaldehyde to L-histidine. In most fungi and in the unicellular choanoflagellate Monosiga bevicollis, the HisD domain is fused with units that catalyze the second and third biosynthesis steps in this same pathway.
Statistics
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PSSM-Id: 119329
View PSSM: cd06572
Aligned: 193 rows
Threshold Bit Score: 327.092
Threshold Setting Gi: 18312330
Created: 8-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residues [active site]
Evidence:
  • Structure:1KAE: L-Histidinol dehydrogenase monomer from Escherichia coli; the catalytic residues line up with a water molecule and the substrate, which in turn participates in the octahedral coordination of the zinc ion.
    View structure with Cn3D
  • Comment:the catalytic His residue participates in acid-base catalysis
  • Comment:the catalytic Glu residue activates a water molecule that attacks the reactive carbon in the second step of the reaction mechanism

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1KAE_A        32 ITRTVNDILDNVKARGDEALREYSAKFDkttv--talKVSAEEIAAASErlsDELKQAXAVAVKNIETFHTAQKlp-pvD 108
gi 116622953  24 AEAVVTPILEAVRKRGDRALMDYARQFDqlqr--ksvRVPEGELLAARDgltPEFRGAIETAAPNIRAYAGRQMpr--eW 99
gi 108803943  30 VRAAVRQVVEEVRERGDEALLEYGERFDgvrp--dplRVPREEIERARAalpGELAEAFRTAIENVRRFHERELdr--sW 105
gi 94970710   27 VEPVVRKIVVDVRKNGDAALRRYASKLDglgk-taalRVSTEELKSAWDatrSEFKQAIRAAAKNIERFARWQMpk--sW 103
gi 3122222    29 ALAIAKEVIEQVKMEGDAGVLHYSRQFDfagataenlRVSEAEFAEAEKlvdPELRRAVEHAFRNIEKVHAGQMpppmhL 108
gi 85542025   32 FIDIVKPIIENVKLNGDKALSEYAKKFDkaevstdqiQVTQAEFDEAFTlvdEEVIQTLSYSIDNIKKFHEAQMpeemwM 111
gi 29336817   26 VLPDVERIFEDVVERGDEALLEYTERFDgvkleaedlRVSEDDFEVARElvdERTVEALEEAAHRIEEFHRKTLprvdrI 105
gi 29336907   31 FVEKVRPIIQAVKDEGDAALIRFARELDkanvaegglQVSEAEFDAAFDkveKDVVESIGFGIDNIRRFHEEQKpetmwL 110
gi 121551851  42 YANAVLPIIEDVRNRGDAALVHYAAQFEgaaigldsiRVSPAEFDAAFAmldPKVVAAIEYGIENIRSFHTAQKpvqywM 121
gi 169197739  33 FIDKVNPIIEAVRIDGDAALADFARQFDkaqvaadqlRATTEEFSAARAsidPELITTLEFAAGNIRHFHEKQMpetlvL 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1KAE_A       109 VETq-pGVRCQQVTRPVasVGLYIPGGsAPLFSTVLXLATPASIAGCKKVVLCSPpp-----iADEILYAAQLCGVQDVF 182
gi 116622953 100 NRSfgaGLQLGQLVRPLdaVAAYIPAGrYPLPSTVMMTVIPAQVAGVPNICIACPk------pVAEVYGTAAMLGVRNVF 173
gi 108803943 106 ELEr-gGARLGQRVRPLrrAGIYVPGGhGAYPSTLVMTAVPAQVAGVEELCVCTPpgpd-graNPRLLAVAGLLGIGEVY 183
gi 94970710  104 TRDagdGIELGQIIRPIesVGCYVPGGrYPLPSTVLMTVVPARVAGVERVVVVSPn------pARETLAAAYLLGVGEFY 177
gi 3122222   109 AEIe-pGVFAGEKITPLptVGLYVPRGkGAFPSMMLMLAVPARVAGVKKIVVCTPpdke-gkvEPVSLVTARMAGVDEVY 186
gi 85542025  112 KQIr-pGCYAGDRFTPInaVACYIPRGkGSFPSVAIMTAVPAIVAGVPTAIIITPpgtd-gkvDAATLVVAKLVGIDKVF 189
gi 29336817  106 TFDv-eGTECGLTLRPIprVGCYVPGGrAAYPSTALMTVIPARVAGCREVVVCTPpadndvraSPEVLVAVEIAGADAVY 184
gi 29336907  111 KEVr-pGAYAGDRYTPIasVALYVPRGkGAFPSVTMMTSVPAVIAGVPQIAIVTPptsd-gsvDAATLVAARLAGVHTVY 188
gi 121551851 122 KELr-pGAFAGERITPIdtVALYVPRGkGSFPSTVMMLCVPAVVAQVPTAYILTPagpd-grvDAATLVAARLAGIETIF 199
gi 169197739 113 HETh-rGVLVGDRWNAIdsVACYVPRGkGSFPSSVLMTAIPAKVAGVRKVVIITPpgpd-gtvDPATLVAAEIAGVSDVF 190
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1KAE_A       183 NVGGAQAIAALAFGtesVPKVDKIFGPGNAFVTEAKRQVSQrldgaaIDXPAGPSEVLVIADSGATPDFVASDLLSQAEH 262
gi 116622953 174 QMGGAQAIAAFAFGtrtVPRADRIVGPGNIYVAAAKKLLAGev---gIDFIAGPTEILIIAADGDPRHFAADMLAQAEHD 250
gi 108803943 184 AAGGAQAVAAMAFGtesVPRVDKIVGPGNDYVTAAKLEVFGev---gVDAPQGPSELVVVADGSARPERVALDLMAQAEH 260
gi 94970710  178 RIGGAQAVAALAYGtesVERVAKIVGPGNIFVTTAKKLVAFd----cAIDMLAGPTEAAIVSERGNARFIAADLVAQAEH 253
gi 3122222   187 KLGGVQALAAIAYGtktVSKVDKLIGPCSIYGAAAKRLLSGiv---dVGLPAGPSESIVLADETTDPKLAALDLLIEAEH 263
gi 85542025  190 KCGGAQGIAAVAYGtntVPKCDKVVGPGSPFVVAAKKLLADii---hPGTPAGPSEAIVLADDTANPKLAALDLLVEAEH 266
gi 29336817  185 RVGGAQAIAALAAGtetVLRVDKIVGPGNVYVTAAKLLAYSrgl-tdVDMPAGPSEVFVIADDSANPDWLARDLIAQAEH 263
gi 29336907  189 KCGGAQAVAAVAYGtetVKPALKIVGPGSPWVVAAKSELSSii---nTGLPAGPSEAIIFADDSVDGGLAALDLLIEAEH 265
gi 121551851 200 KVGGSQAVAAAAFGtstIPKADKILGPGSPWVMAAKRALVDhl---dPGIPAGPSESIVFADASADARLAALDVLIEAEH 276
gi 169197739 191 KCGGAQAIAAVAFGtqtVPKCDKVVGPGSPWVVAAKKQLSSli---dPGSPAGPSELIIFSDGSVPAELVALDLCVESEH 267
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                           ##           
1KAE_A       263 gpDSQVILLTPAADXARRVAEAVErqlaelpra----etarqalnaSRLIVTKDLAQCVEISNQYGPEHLIIQTRNARe- 337
gi 116622953 251 v-DASAILLTTSKRLAAAVSRELErqla--------------ilptAPVASKSIAKNSAIILVRSLDEALEISNRFAPeh 315
gi 108803943 261 lsGATSVLLTPSGALASGVEPLLSgrp----------------sehITLVLVRDLEQALEISNLYAPEHVHLNCEGAGa- 323
gi 94970710  254 dpETMVAFFTTSPSLADAVKKQVAlla-----------------akNTIARKALATRGYIFVAQDIAQAMEAANQFASeh 316
gi 3122222   264 gsDSAALLVTHSASLAEKALGYLGeyleklppwrk-kfcedglgsyGGILLTDSLQASLDFINDYAPEHLQVLTADPLk- 341
gi 85542025  267 gpDSSAFLVTNSKELAEQAQVAINeywqhmdrlrvdfsstvlsgdnGGIVLTSTFEEAVDFCNDYAAEHLLILSKSPFd- 345
gi 29336817  264 dpHAAAVLATDSEEIARAVKERVEelldagiere--eivlkaldrnGWIVVLDSLEECVRLANRYAPEHLQLCVENPEe- 340
gi 29336907  266 gpDSSAYLVTHSRKVAEAALAALPqhwsrmteqrvefsravltgkrGGIVLTASLEDSYRFINDYAPEHLEILSKEPFa- 344
gi 121551851 277 gpDSSAFLVTTSRAIASAAIDWIAhfwnemsarrvefsatvltgprGGVLLADSVPSAMDFINDYAPEHLEILGAEPFg- 355
gi 169197739 268 gpDSSVFFVTDNADFANAVASCVPalwsrmgniraqysktvlsgprGGIVIARTRADALAFVNDYAPEHLAVLADNAWq- 346
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1KAE_A       338 -------LVDSITSAGSVFLGDwSPESAGDYASGTNHVLPTYGYTAtCSSLGLADFQKRXTVQELSkegfsalastietL 410
gi 116622953 316 lsipdasLLPGVRHAGSVFVGPySPEAAGDYASGPNHVLPTSGAARqRGGLSVTDYVKVISVQNLTepalrrlapsittL 395
gi 108803943 324 -------ALERVEAAGLVAVGDlSPVALSDYAAGPSHALPTGGTARwASALGTHDFLTRINHMRFSrealrelgphverL 396
gi 94970710  317 lt-vdedLLETVSSAGSVFVGDySAQSFGDYASGPNHVLPTGGLARvRGGLSVLDYVKIITVQKISrkglralapiaetL 395
gi 3122222   342 -------LVGKIDNAGEILLGNyTPSSAATYAIGVNAVLPTGGFARsYSAVSVFDFLKRSTLAYLTeegfagvketvttL 414
gi 85542025  346 -------HLGKIINAGEILLGEnTPISIANYTLGPNAVLPTSMAAKtASPLSVFDYLKSCSIGYLTregyeelaphtyrF 418
gi 29336817  341 -------LLQDVENAGAVFVGHlTAVPFGDYATGPNHVLPTGGFARaRGALGTWDFVKIIPIQRLRegdverlapiveeL 413
gi 29336907  345 -------HLGHITEAAEILMGPhTPVTLANFVLGPNAVLPTSRWARtYGPLSVTDFVKRSSVGYVTsaaypelakharrL 417
gi 121551851 356 -------YLHQIRNAGEILLGHaTPMSIANFVLGPNAVLPTSFGAKtVSALSVHDFIKRTSIGYVTtkafppmaamgreL 428
gi 169197739 347 -------YLACFEHAGEILLGPhSAISVANFVLGPSHVLPTGGAAKtTSPLSVFDFLKRTSIASLSkeayggfaahaerL 419

                 ....*....
Feature 1                 
1KAE_A       411 AAAERLTAH 419
gi 116622953 396 ARAEGLEAH 404
gi 108803943 397 ARMEGFENH 405
gi 94970710  396 AAAEGLVAH 404
gi 3122222   415 ADYEDFPAH 423
gi 85542025  419 AKYEGFDAH 427
gi 29336817  414 AEREGLPNH 422
gi 29336907  418 ARYEGFSSH 426
gi 121551851 429 AIYEGFDAH 437
gi 169197739 420 ARYEGFDGH 428

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