Conserved Protein Domain Family
TM_PBP1_branched-chain-AA_like

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cd06574: TM_PBP1_branched-chain-AA_like 
Transmembrane subunit (TM) of Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which are involved in the uptake of branched-chain amino acids (AAs), as well as TMs of transporters involved in the uptake of monosaccharides including ribose, galactose, and arabinose. These transporters generally bind type 1 PBPs. PBP-dependent ABC transporters consist of a PBP, two TMs, and two cytoplasmic ABCs, and are mainly involved in importing solutes from the environment. The solute is captured by the PBP which delivers it to a gated translocation pathway formed by the two TMs. The two ABCs bind and hydrolyze ATP and drive the transport reaction. This group includes Escherichia coli LivM and LivH, two TMs which heterodimerize to form the translocation pathway of the E. coli branched-chain AA LIV-1/LS transporter. This transporter is comprised of two TMs (LivM and LivH), two ABCs (LivG and LivF), and one of two alternative PBPs, LivJ (LIV-BP) and LivK (LS-BP). In addition to transporting branched-chain AAs including leucine, isoleucine and valine, the E. coli LIV-1/LS transporter is involved in the uptake of the aromatic AA, phenylalanine. Included in this group are proteins from transport systems that contain a single TM which homodimerizes to generate the transmembrane pore; for example E. coli RbsC, AlsC, and MglC, the TMs of the high affinity ribose transporter, the D-allose transporter and the galactose transporter, respectively. The D-allose transporter may also to be involved in low affinity ribose transport.
Statistics
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PSSM-Id: 119320
View PSSM: cd06574
Aligned: 25 rows
Threshold Bit Score: 128.164
Threshold Setting Gi: 160940484
Created: 8-Jan-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
TM-ABC
Feature 1:TM-ABC transporter signature motif
Evidence:
  • Comment:a signature motif (the EAA motif) conserved in all TMs of ABC transporters.
  • Comment:a conserved hydrophilic segment, part of a loop which interacts with the cytoplasmic ATPase.
  • Citation:PMID 7934906

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 84027817   19 GSTYALIAIGYTMVYgiIGMI----------------NFAHGEVYMIGSYvsfmiiaalmmmgidtGWLLVAAGFVGAIV 82
gi 585411    118 TMIYIILGLGLNVVVglSGLL----------------VLGYGGFYAIGAYtfalln----hyyglgFWTCLPIAGLMAAA 177
gi 81344761   15 STPILITALGGLFSEr-SGVI----------------NIALEGLMMFGAFstatvtvlcepytiaaPWIALGVGMAVAAS 77
gi 19705201   24 ISRNLFLIMSLLIPIv-AGMG----------------LNFGIVLGAMGGQlalilitnwhimglqgIFLAMILSIPFSIL 86
gi 6647971    11 SLIMLPLILGMYISYriLKIT----------------DLTVDGTYVLGAAvfart-------ipfgLFHALIFAIIAGGI 67
gi 91781119   18 GLTYALIAVAIMIPFriLNFS----------------DMTGEGAFPFGACvcakll-----migfnPVMALLGGALAGFV 76
gi 94986860   13 GLILAIIAFGIMIPFrfLNFP----------------DLTAEGAYPLGGAvcaslm-----vanmpQILAMMAGIMAGGL 71
gi 160940484 105 GPVSFFMAILIATPFa-LIFGggyg---------wllNKIKGGEMMIATYv---------------GFSSVSFMCMMWLL 159
gi 160880191 194 MLLLLRILLVLYFVFsaANVIlrkkkegksgyklsiiKISVTILLLLLTLipair------dflgtDRLLLLNAITIGCV 267
gi 160940485  52 LGRNAFLILSLLIPIm-AGMG----------------LNFGMTLGAMAGEiglifvadwqiwgipgIILAMIISIPISIL 114
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 84027817   83 IASAYGw-------SIERVAYRPV-------------RNSKRl----iALISAIGMSIFlqnyvsltegsrdvalpslfn 138
gi 585411    178 AGFLLGf-------PVLRLRGDYLaivtl----gfgeIVRILllnnteITGGPNGISQIpkptlfglefsrtareggwdt 246
gi 81344761   78 VALFYAy-------LSVRLCSDQI-------------IAGTA------INLCATGMTVFfaqvifgqqgtqaysrglvkt 131
gi 19705201   87 LGYIGGv-------ILNRAKGKEMi------------TSMILg----yFINGAYQLVVLysmgkifpvkdktlllssgrg 143
gi 6647971    68 NGSIVSf-------MQRNKRINGL-------------IAGIL------ANFMLYSVNLQimqrpnisvlgmpt------- 114
gi 91781119   77 AGVLTGy-------IHEKAKINTL-------------LCGIL------VLSMLYSVNIRvmgqpnsalfayssvf----- 125
gi 94986860   72 LAVGTSq-------VALRLKVNSL-------------LAGII------LSTMAYSINLRimgkpnialfd---------- 115
gi 160940484 160 LPYSSPt-------MVWGLSGKGLrttislegfydkaLANFLq-----INIGKISIPTG--------------------- 206
gi 160880191 268 LAILYVitkicsikLIQKHKELEVnk----------yLAMIIia---iIVYALTYIPVVfditms--------------- 319
gi 160940485 115 LGLFCGk-------MLNMAKGREMv------------TSYIIs----fFMNGLYQLVVLymmgsiipiihssiklprgyg 171
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                     ###################
gi 84027817  139 g--------qwvvghsenfsasittmqaviWIVTFLAMLALTIFIRYSRMGRACRACAEDlKMASLLGINTDRVIALTFV 210
gi 585411    247 ---------fsnffglkydpsdrviflylvALLLVVLSLFVINRLLRMPLGRAWEALREDeIACRSLGLSPRRIKLTAFT 317
gi 81344761  132 s--------ygffsripvlgpmvfthtyptVYLGFVLVALAWYVLYRTPFGVHVRATGDQpYAVDGAGLSVFRLRSAAVV 203
gi 19705201  144 ikntvdltevaksidnaiplrifrydipvlTILFIIALCFFVIWFRKTKLGQDMRAVGQDmEVSKSAGIEVNKVRIYAIV 223
gi 6647971   115 -----------------llsildldnwlvpLILINSFIIVIVLILLKGNLGLFLRAFGFNkDLLIDLGKPAELYRMLGLS 177
gi 91781119  126 --------------swlpeagggylprilmLTVIDAVIGAGVLWFLGTQHGLAMRAIGSSpSMAKAQGINVKVYTLVGLG 191
gi 94986860  116 --------------------mdgihfdfisLIIVGMFCIVPFALFLHTDFGLRFRTVGSNpKFAINHKISINKYTSLGLF 175
gi 160940484 207 ------------------------------SLLFFAFLAFLMWAFLHTKTGTAMTAVGSNpNFARASGINIDKTRMLSVI 256
gi 160880191 320 ------------------------islpvcTYAAIILICGFNRWLLSTKLGQNMRTVGQSrTVANSAGINVNKTRIIAMC 375
gi 160940485 172 vrntvsllhmrqyldnllaihiggvkipvlTLIIIALLCLFIIWFRKTKLGQDMRAVGQDmDVAGDAGIKVERTRIIAII 251
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 84027817  211 IGAAMAAVAGVLLGQFYGvi-npYIGFMa-GMKAFTAAVLGGIg------------SIPGAMIGGLILGIAEAl------ 270
gi 585411    318 ISAAFAGFAGTLFAARQGfvspeSFTFAe-SAFVLAIVVLGGMg------------SQFAVILAAILLVVSREl------ 378
gi 81344761  204 ISGLCAGLGGGVLILTQDiqytvYSTHGt-GFIALAALISGRW-------------HPFGVLVTSVLFGFSQIl------ 263
gi 19705201  224 ISTVLAGIGQVIYLQNLGtintyNSHEQi-GMFSVAALLIGGAsvara----tipnAISGVILFHTMFVVAPRag----- 293
gi 6647971   178 ISNGLAALTGTLSAQVNGfa-diNMGYGv-ALVGIGAIIIGRQiflnninnfnalkEIFACFIGILFYFISLSi------ 249
gi 91781119  192 LANLFAALGGAMLAQNQGfa-dvNMGFGv-LVNGLAALLLGEAvvgnr----smlrQVLAPILGSIIYFQLISi------ 259
gi 94986860  176 IAGSMFGLAGSLMVQMQQfm-diGMGVGi-VIHGLASLMIGESivgns----tipkQLAAPIIGSLIYQQIQGv------ 243
gi 160940484 257 MSTWLAAVGILMYEQGFGfi-qlYMAPFymALPAVSAILIGGAsvn--------kaSITNVIIGTFLFQGIVTmt----- 322
gi 160880191 376 ISTVLASWGQLIYLQNLGtfstyGAHSMv-GLYAIAALLVGGAgvqma----nnkqAILGVILFHTLFVVAPLsa----- 445
gi 160940485 252 MSTVFAGLGMVIYLQNMGnistySSHTQi-GMFCIAALLVGGAsvdra----signVFLGVILFHLMFIVAPKagatitg 326
                        330       340       350
                 ....*....|....*....|....*....|...
Feature 1                                         
gi 84027817  271 --SSAYlst--------eyKDVVSFa-LLILVL 292
gi 585411    379 --MRDFne----------ySMLMLGg-LMVLMM 398
gi 81344761  264 --NVYAtsvellkhlpielFSALPYa-LTVVVL 293
gi 19705201  294 keLMGSaqig------eyfRVFISYg-IIALVL 319
gi 6647971   250 --LLHIgid-------pinLKLILGi-VLFISL 272
gi 91781119  260 --VLAVgfq-------psdLKLVTA--LFVLVT 281
gi 94986860  244 --ALSLgla-------psdLKFFTGsiVLIVLA 267
gi 160940484 323 ptVMNSmihmd---mseviRIVVSNg-MILYAL 351
gi 160880191 446 snLLGSslig------eyfRVFICYg-VIALSL 471
gi 160940485 327 dsMIGEy-----------fRVFISYg-VITVAL 347

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