Conserved Protein Domain Family
TM_PBP1_LivM_like

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cd06581: TM_PBP1_LivM_like 
Transmembrane subunit (TM) of Escherichia coli LivM and related proteins. LivM is one of two TMs of the E. coli LIV-1/LS transporter, a Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporter involved in the uptake of branched-chain amino acids (AAs). These types of transporters generally bind type 1 PBPs. PBP-dependent ABC transporters consist of a PBP, two TMs, and two cytoplasmic ABCs, and are mainly involved in importing solutes from the environment. The solute is captured by the PBP, which delivers it to a gated translocation pathway formed by the two TMs. The two ABCs bind and hydrolyze ATP and drive the transport reaction. E. coli LivM forms a heterodimer with another TM, LivH, to generate the transmembrane pore. LivH is not included in this subgroup. The LIV-1/LS transporter is comprised of two TMs (LivM and LivH), two ABCs (LivG and LivF), and one of two alternative PBPs, LivJ (LIV-BP) or LivK (LS-BP). In addition to transporting branched-chain AAs including leucine, isoleucine and valine, the E. coli LIV-1/LS transporter is involved in the uptake of the aromatic AA, phenylalanine.
Statistics
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PSSM-Id: 119323
View PSSM: cd06581
Aligned: 387 rows
Threshold Bit Score: 84.4177
Threshold Setting Gi: 171185691
Created: 9-Sep-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
TM-ABC
Feature 1:TM-ABC transporter signature motif
Evidence:
  • Comment:a signature motif (the EAA motif) conserved in all TMs of ABC transporters.
  • Comment:a conserved hydrophilic segment, part of a loop which interacts with the cytoplasmic ATPase.
  • Citation:PMID 7934906

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 585411    118 TMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYtfalln----hyyglgFWTCLPIAGLMAAAAGFLLGf---PVLRLR 190
gi 33596923  332 ILIAALFAVSLHFMMGPGGMHSFGHAAYFGLGAYgaalll----kaaslpMEAALAFAPLVAALGALIFGw---FCVRLS 404
gi 27378937  339 IAIFVIFAVSLHFLMSVGGLASFGHAAYFGLGAYgiafla----kmaglpMIVCLLLGPLLGCMGAAVFGf---FAVQLS 411
gi 77404666   50 TLIQAVFALSWNLLFGFTGLASFGHAGFFAIGAYftgaalr---ydfhlpFLAVLAVAGLLGALVALAIGv--vALKRLT 124
gi 27375306   66 VIILALFALSFDLVWGYAGIMSFGQALFFGSAGYgvallar---dlditnIFLVLPAGTIIGLTFALLLGg--fLLLGRH 140
gi 91781257   36 VLILSLLALSFDLVWGYAGILSFGQALFFGGGGYvaavlar---dygvnaAIVALPAAILAGALLSAVVAgvvvLGKSAT 112
gi 121604522 344 ALILMIFGISLQSMMALGGLVSFGHAAFFALGAYgaalsh----slwgatLPVALATGCGAALAVAGVFGa---AVVRSS 416
gi 198254924  36 FMVWTFMAMGLGIVWGYCGVLSFGQTAFFGLAGYaygvvsin-lgaafglTWLALVLSVTIGGAFALLIGyf-mFYGRIK 113
gi 187920438  42 CLLWAFSALGLAAMWGYGGILSFGQTAFFGLSGYtygiftln-ygdtwfeSWLGLGAGIVVSIVAAGLIGym-vFYGRIK 119
gi 198253797  39 WIIFGLLGLSLDVIWGRGGLLSLAQTAFYGLGGYfgaviainmapsfgmtLVWALPAAAVFGALIAAALGyi-iFYSRMG 117
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 585411    191 GDYLAIVTL---GFGEIVRILLlnn--------teITGGPNGISQIPkptlfglefsrtareggwdtfsnffglkydpsd 259
gi 33596923  405 GVYLAMLTL---AFSQIVWSVVfqw--------ddFTGGSNGMIGVWpaewlsg-------------------------- 447
gi 27378937  412 GVYFAMLTL---AFAQIVWSIAfqw--------vsVTGGDNGILGVWpekwaas-------------------------- 454
gi 77404666  125 GIFLAVLTV---ALTEAMAKIIgf---------spALGEQDGLGNIPrpviemglfqi-------------------dlt 173
gi 27375306  141 PSSVIFVSLgtlTGSYAADRLArg---------wyYLGGQNGIPSIApmtlggyef-----------------------s 188
gi 91781257  113 QIFIALGTM---TASYAAYRLAis---------wyYLGGQNGISSLPllktgsyef-----------------------d 157
gi 121604522 417 GVYLAMLSL---ALAQVVWAGAaqw--------vsLTGGDNGLIGLAlvtddgr-------------------------- 459
gi 198254924 114 GVFIGIVTL---SVTLVLETFMaqtagpnwrigdaRLNGFNGMSGMPpltvpwpggdi-------------------vly 171
gi 187920438 120 GVFIGIVTL---SVTLVLETFMsqtagpqwaigeaRLNGYNGMGGMPqltipwpggpl--------------------tl 176
gi 198253797 118 ELQSTILSY---TFTLLLWSVTqtfkl---dvgeaTIGGDNGLSNIPgiilgfgaeag--------------------kl 171
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               ###################                      
gi 585411    260 rviflylvALLLVVLSLFVINRLLRMPLGRAWEALREDeIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQGFVSP 339
gi 33596923  448 --tayyylALAVVAAAVLGLRRVLFSPFGYAMRAGRDSsLRADAIGIDVKRVQWAAFVVAGAFGGLAGALYAFSKGSISP 525
gi 27378937  455 -pshfywlALGVAALVTVALRAMVFSPFGYALRATRDSlLRSEAIGINAKRIQWTAFVIAGTTAGIGGALFAYLKGSVFP 533
gi 77404666  174 hgddyyrfLVVATVLIVAALWWVVHSRLGRTFRAIREDaDRARFVGIDVARYRVISFMISGGTAALAGALSAPLTRIVTL 253
gi 27375306  189 egpafyylVLGILVVVYLLCRFLVRSQFGLALAGLRENeQRIAFFGYKAQHLKAIIFTIGGGVAGLAGSLYAFHEGFVWP 268
gi 91781257  158 egprfyylALALLIVIYAVSRFLVRSQLGLALTGIRENvRRMVFLGYRVNVFKAAVFVFSGAIAGLAGGLTAFHEGFIGP 237
gi 121604522 460 --pvfyalLVALALIAVFALRFLSRSVMGAALQAVRDApLRAAASGLSVGWLKYRVFVESAVLAGLAGGLFAAHKGAVFP 537
gi 198254924 172 sgtalyfvLLVMVVVCYLGLRMLLNAPFGNVLVAIRENpERAEMLGYDIRLYQTLAFGLGGALAALSGTLYTAWGQYITP 251
gi 187920438 177 envsfyylVLLLLIAVYAIMRKLLDGTFGLTLIAIRENpQRAEMLGVDIRRHQLMVFVLGCTLGGLSGALYTIWGSYITP 256
gi 198253797 172 kpngafatVVVVSAAVYFFTHWLMRSNFGKVVDCIRIDiEKTELLGYDIRKIQVLNFAYSGAIAGIAGALFALWANYLNP 251
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                              
gi 585411    340 eSFTFAESAFVLAIVVLGGMgSQFAVILAAILLVVSRELMRDFne--ySMLMLGGLMVLMMIWRPQGLLP 407
gi 33596923  526 dTMAVSKSVDGLVMVLLGGIqTLIGPVVGATAFNVLQDYIMRAte--yWRALFGGVILLLVLVFPQGIAG 593
gi 27378937  534 dNLGISLSVDALVMVLLGGVeTVSGAVIGAIVYKALNIWLVSQtd--lSKLVLGGFIVLIVVVFPKGIVG 601
gi 77404666  254 dQVNWLASTQPILNSLLGGFtSFWGPIVGSAAFSTINYFTRGFpg--lSEIMVGGILVVVILVAPTGILG 321
gi 27375306  269 nMVGVVVSTQVVLYVLFGGSgTLIGAVIGAIIVEGVSFWLSDNyr-diWPIILGLLLLLVILFRPLGLIS 337
gi 91781257  238 gSLGTVLSTQAVLYSLLGGSgTLIGAVFGAVIIETLGFGLSDRlp-elWPVLLGIILLLLIIYRPQGLIS 306
gi 121604522 538 sLAAVSTSVDALLVVLLGGVhQLWGAVVGSLVLTYASAELGREvt--yWRGLLGVFIMLIMVASPSGLLG 605
gi 198254924 252 sSMGLTAAALPIVWVAVGGRrDITATLLGSILVIAGFQTLTIYgs-qyALIVMGALLVFTVLVTPDGLIS 320
gi 187920438 257 sTMGLTAAAMPVIWVATSGRkSIGGTIIGTAFLVWLSQNLAVYgs-qyALILLGAILLIVVLAAPEGLLP 325
gi 198253797 252 sIFSVQEALLIPIYVLVGGLgTLAGPFLGAIVIGALSFWLGGGaaggqTTLILGAILILLVLFLQNGFLG 321

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