Conserved Protein Domain Family
TM_PBP1_LivH_like

?
cd06582: TM_PBP1_LivH_like 
Transmembrane subunit (TM) of Escherichia coli LivH and related proteins. LivH is one of two TMs of the E. coli LIV-1/LS transporter, a Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporter involved in the uptake of branched-chain amino acids (AAs). These types of transporters generally bind type 1 PBPs. PBP-dependent ABC transporters consist of a PBP, two TMs, and two cytoplasmic ABCs, and are mainly involved in importing solutes from the environment. The solute is captured by the PBP, which delivers it to a gated translocation pathway formed by the two TMs. The two ABCs bind and hydrolyze ATP and drive the transport reaction. E. coli LivH forms a heterodimer with another TM, LivM, to generate the transmembrane pore. LivM is not included in this subgroup. The LIV-1/LS transporter is comprised of two TMs (LivM and LivH), two ABCs (LivG and LivF), and one of two alternative PBPs, LivJ (LIV-BP) or LivK (LS-BP). In addition to transporting branched-chain AAs including leucine, isoleucine and valine, the E. coli LIV-1/LS transporter is involved in the uptake of the aromatic AA, phenylalanine.
Statistics
?
PSSM-Id: 119324
View PSSM: cd06582
Aligned: 381 rows
Threshold Bit Score: 87.4864
Threshold Setting Gi: 196237274
Created: 9-Sep-2008
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
TM-ABC
Feature 1:TM-ABC transporter signature motif
Evidence:
  • Comment:a signature motif (the EAA motif) conserved in all TMs of ABC transporters.
  • Comment:a conserved hydrophilic segment, part of a loop which interacts with the cytoplasmic ATPase.
  • Citation:PMID 7934906

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 84027817   15 GVTLGSTYALIAIGYTMVYgIIGMINFAHGEVYMIGSYvsfmiiaalmmmgidtGWLLVAAGFVGAIVIASAYGw----- 89
gi 83594858  157 ALPVIALYGLLAASYTLIYaLGRTILLVFGELAMIGAMacfagv-----alfsgLGGLGTAGVVGGAVVFALLAsav--- 228
gi 13474313   11 GLHSGALYALLAFGYVLINgILHRTNLAYGALFAFSGQamilsv----afgyqvLWLTLLVSVLFGVVAAFLYAali--- 83
gi 158423166 151 ALPNMTAYAALAAAFTLIHgLTGRIILAMGEIAVAGGGamlava-----alapaTGAFTLAHLLLGLVAGTATGglw--- 222
gi 159184625  11 AVPVAALYAVLAFGYAIAFsVTKRADVTYGAIFAFAGQtcll--------fadfGWNRLWLVLPATLALGAGAGlfgglw 82
gi 119716378  11 GITSGAVYGLLAVAICLTYrVSKVINAAIGELMVLGALiass---------ligRGWPLAIAVVVGVGVVGLVAa----- 76
gi 158315028   8 GLALGSIYAIASSALVITYvSTGVFNFAFGSMAFFVARfyyw--------lnseHGMSTVSAGLLSILVMAPLLg----- 74
gi 192289959  12 GALIGAIYGLLAFPVSLLFvTTETVDLAVGAYAVLAAAv--------------aASFAAPLGIALGIAAAVAASl----- 72
gi 158316502  11 GAVAGGLYAILAAGVVLTYqTSGIFNFAHGAVAFATAYlfvq--------lnvtAGVPVVPAAIISILVFAPLLg----- 77
gi 158315823  27 GVPPGAVYALVALGFVLTYkTSGVFNLAFGAQAYVSAAmyfk--------arviWEWPTVPSVILAVFVMAPLIg----- 93
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 84027817   90 ---SIERVAYRPV---RNSKRl-IALISAIGMSIFlqnyvsltegsrdva-----------lpslfngqwvvghseNFSA 151
gi 83594858  229 hgdALRRLVLQPA---AGRTPl-ALLVASFALAIVvqdyvrlsqdagdrl-----------lptlpgrthvlaragEFVV 293
gi 13474313   84 -shVLSRSVFEPL---ADRSPnaIVVTTLGILIVLseasriaadthdlwl------------ppmlaqpivfaegaGFKA 147
gi 158423166 223 -mlTFGRLVLRRMaerREASQa-VIIASIGLALVLreamtlaqsgsglwl------------prlvhtpiplagsaAFTA 288
gi 159184625  83 aagLIGRAVMRPL---AKASPn-AVTVASIGVLIAltesarlaadtrqlw-----------lppllsqpvrfwiegGFAV 147
gi 119716378  77 ---AFSGIILRPAi-kAKSTMs-QLLLVTFSLSILlqgvmlvaygrdi---------------hsapawiggdgmtIGGV 136
gi 158315028  75 --vLLYGALFRFM---RGSSMl-VKIVSTIGLSVAlppisdlifgtdtit------------sapglaslndkpyhVFGT 136
gi 192289959  73 ---VVAVLSLRTGastAKDPLa-IVLMTFGVSVFLesmvltgfgqnp------------------wirqpfetfwlAGPL 130
gi 158316502  78 --lLLDRLVYRRL---AHAPLa-VKIVVPIGLLIAvpglclfissrlnlwfhldiagiedlflipglgptpkktwtLGGV 151
gi 158315823  94 --lILERLIFRPL---RTAPAvaRLVVAAGLAVAIpylfdiladftavagv---------tpvgvvpdganvfydpFGVY 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                ###################                     
gi 84027817  152 SITTMQAViWIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGvin 231
gi 83594858  294 AVTDFQLVlVGGAGIVLGALVWGMGRGRFGRCWRAMAEDPQMATLCGVSKTRLWAICFALAATLAGLAGVVVLLRFGlan 373
gi 13474313  148 TLTLIQVLdCAVVLAAVALATSAFARSSFGRRWRAVSDDPRAAALCGVDVRGVFRHAVLLGGFCAALAGVMASLYYGnvs 227
gi 158423166 289 TVTPAMIGgALASGCGVAGVLALLRFSRFGRGWRAFRDDPLMASLCAVAPSRLLGATFLLAGALSGLAGASLVLVFGnvd 368
gi 159184625 148 TLTPIQMLnTAVFCLLILTGSLYLGRSAFGRRWKAVSDDAFAASLLGVNASRIFLLSYCAAGLVAAIAGVLATFYYGtmd 227
gi 119716378 137 VLQPQRLLiLAATLALGTGLVLYFSRSVHGRAMTATSQNPVGAALVGISVSRFRDVAFVGTAVIAALLGCMLIPITPf-v 215
gi 158315028 137 VVTTDQLItYGFLVFTVVAGTAVLRLTDVGLRVRAMVDSEAMASLSGTNPGRVSLGVWAASSTLAGLAGILVAPTNGl-n 215
gi 192289959 131 RISPQGAVnVGVGVALMAGLYVWLYRTAAGRDMRACAANAIAATLAGIPVRRITLMTHVIGGLLAGIAGVLILYTTGa-s 209
gi 158316502 152 LLDSNQIAvFGAAVLTAVGLWVLLQRTRLGLQMRAVVDRRSLAALRGVDPDRTSAVSWMLGSFLAGLAGVLVAPIFT--- 228
gi 158315823 160 AYSRNELVsMGVALVAMAGLVALFRFSAIGVRMRAVVESPKMTELNGIPADRVSALSWALSSLFAGVAGVLIAPRFNt-l 238
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 84027817  232 PYIGFMa--GMKAFTAAVLGGIgSIPGAMIGGLILGIAEAl-SSAYLst-------------eyKDVVSFa--LLILVL- 292
gi 83594858  374 AFMGAMl--GFKALTAAVVGGIgRIQGAILGAVVIGGLETy-WAAYFdm-------------awRDVAVFa--LLAAAL- 434
gi 13474313  228 FGSGLVy--GLKILFVTAVGGYlSPPRAALGAAAFGMAESl-WAGYFpi-------------ewRDAWMYl--FLVAMLi 289
gi 158423166 369 AALGLPl--TLKTLAGAILGGVgSVGGAAAGGLLVGALEGgwSSVWDia--------------wRDVAIYg--LLILGL- 429
gi 159184625 228 FGAGLVf--GLKVVLISAAGGYaSPLICGLGAAAIGFAETf-WVGYGpv-------------awRDAAVLa--LLVGWLi 289
gi 119716378 216 YNAGLPl--ALVGMVGATLARFvNIGVAMAAGIVIGVLEQm-IARYVsa-------------dlQTSLVY---IVLTVLm 276
gi 158315028 216 TGGMTSl--MCAAIAAVVAARLrSLPGAVAAALAMGVITDv-IQKYLptnst-------ltaaiVPSIPFa--FMLVFLv 283
gi 192289959 210 YSSSLGl--TISGFGAAAILGLhSPLRSFFGGVAIGMAEAl-AVGYLpv-------------glGAAAPLl--FILLVLi 271
gi 158316502 229 LNTPVFttvVLISTPAVVFARFrSMPLALAGGLLIGVLQNl-IVGYAdfaqn--------isgfSTSVPF---ILLLGLl 296
gi 158315823 239 AAADFFs-lMVVAIAAAAVGRLtSLPKAMAGGLLLGIIIAq-LNTFLprwsddhawvstiqdnlTPSVPFvvlFGVVVLv 316
                        330       340
                 ....*....|....*....|....*....
Feature 1                                     
gi 84027817  293 ---------------------LVMPTGIL 300
gi 83594858  435 ---------------------VLRPQGLV 442
gi 13474313  290 l------------------igAGRDTGKV 300
gi 158423166 430 ---------------------VARPEGLF 437
gi 159184625 290 ---------------------VMRSRVEA 297
gi 119716378 277 ---------------------LAVPSAFA 284
gi 158315028 284 fyvvrsgsvdenagaggpldaAIRPASQD 312
gi 192289959 272 tg----------------kanIGHAGGRA 284
gi 158316502 297 fv-----------------faVDRSRRAG 308
gi 158315823 317 p-------------------sIRRSRETG 326

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap