2Z7E,2QQ4,1XJS,1SU0


Conserved Protein Domain Family
IscU_like

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cd06664: IscU_like 
Click on image for an interactive view with Cn3D
Iron-sulfur cluster scaffold-like proteins
IscU_like and NifU_like proteins. IscU and NifU function as a scaffold for the assembly of [2Fe-2S] clusters before they are transferred to apo target proteins. They are highly conserved and play vital roles in the ISC and NIF systems of Fe-S protein maturation. NIF genes participate in nitrogen fixation in several isolated bacterial species. The NifU domain, however, is also found in bacteria that do not fix nitrogen, so it may have wider significance in the cell. Human IscU interacts with frataxin, the Friedreich ataxia gene product, and incorrectly spliced IscU has been shown to disrupt iron homeostasis in skeletal muscle and cause myopathy.
Statistics
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PSSM-Id: 143480
View PSSM: cd06664
Aligned: 206 rows
Threshold Bit Score: 46.4737
Threshold Setting Gi: 87199971
Created: 8-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitetrimerization
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:This conserved cysteine cluster is the putative site for the assembly of the nascent Fe-S cluster.
  • Structure:2Z7E_A/B: a single [2Fe-2S] cluster is bound to the asymmetric trimer of Aquifex aeolicus IscU, contacts at 4 A.
    View structure with Cn3D
  • Comment:In the A. aeolicus IscU trimer, the [2Fe-2S] cluster is buried at the interface between two subunits, the third subunit further stabilizes this interaction.
  • Structure:1XJS: Bacillus subtilis IscU binds zinc, contacts at 4 A
    View structure with Cn3D
  • Structure:1SU0: IscU from Streptococcus pyogenes binds zinc, contacts at 4 A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                        #                          #                    
2Z7E_B         4 EYNEKVLDHflnprnVGVLedangVGQCGNPaCGAAMLFTIKVnpendVIEDVRFKTFGCGSAIAVSSMLTEMVKGk--- 80
gi 159899050   5 EAIEFLLEHyeqprhRGELadpdvTMPGGNPgCGDVVTIYLKVdaandAIQDLAFVGEGCTISQAAASILLEQMQGqa-- 82
gi 156740336   5 ALIARLLDHyehprnRGSLpdadvTQTGGIPdCGDVVTVYLKIdp-pdRVVAISFEGQGCTISQAAASILSERVIGstfa 83
gi 108805013   5 LQIQILLDHyerprhRGRLeeadvRMPGGNPgCGDVVTIHLKGaddhrHIEDVTYEGEGCTISMAAASMILEEVHErn-- 82
gi 221633157   4 STFELLRDHvrnprhHGPLedatiVLEGGNPeCGDVVTVYLKIapdgeTIEDVHFTGQGCGVSQAAASLLMERLHQgh-- 81
gi 212632948   1 MYNDIIIDNfsnpkfVGDLesadyKFEIGNPvCGDRIHMQVSFdg--dNIRESSFRAWGCATSVATANIFCDSIIGksid 78
gi 169631747   3 RFSPAVIDHftnprnAGRLdqadvTAFIGNPvCGDQILLSAQIrd--eVVSRIGFEAYGCSASLAVASILTERLSGmpvr 80
gi 37528490    1 MFNNIIIDNfcnpdcQGTLsaptiKLALGNPvCGDKVDIDLTLdn-qgRVNNACFRAWGCTASLAMSNQFCRHAKGk--- 76
gi 121609935  10 MFNEIIVKNfsdpafASELdeataSIEIGNPvCGDRIRVQLDVtd--gLIKRARYQAWGCATSLATGNVFCACIDGk--- 84
gi 163849017   9 DAIERLLDHyrqprhYGELpd-aiTYSGGVPdCGDTLTVYLRVap-ngRLTDLQFTGQGCSVSVGTASILIEQLQGatva 86
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
Feature 1                                        #           
2Z7E_B        81 -----pIQYALNLTykdifee-lgglppQKIHCTNLGLETLHVA 118
gi 159899050  83 ----laAIEALDFNwmidel--grevvqSRPRCATLALGTLKAA 120
gi 156740336  84 eieaitLDEMIDFLgre--------avqTRPRCATLALRTLKAA 119
gi 108805013  83 ----ltMDEVLRMDynemidklgrqivaSRPKCATLGLGTLKAA 122
gi 221633157  82 ----wtIGRIAAVDfslvqdlvgpeaarSRPRCASLALSVLKAA 121
gi 212632948  79 ---eisMRQADEISdmlg------elepSQQHCINILSELHREL 113
gi 169631747  81 --dietIQAAQVIEwsgg-------lspEQQHVAALGADVAQRL 115
gi 37528490   77 -----tLEELNALSpegidal-lgelepAQQHCLDMLHKLFEQL 114
gi 121609935  85 -----pLSVVLDTSnddietm-lgelepSQHHCLEMLRALFQWL 122
gi 163849017  87 --dllaLDETAAVQivgs------evfqARPHCATLIFRTLKAA 122

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