Conserved Protein Domain Family
PLPDE_III_DSD_D-TA_like_1

?
cd06812: PLPDE_III_DSD_D-TA_like_1 
Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzymes Similar to D-Serine Dehydratase and D-Threonine Aldolase, Unknown Group 1
This subfamily is composed of uncharacterized bacterial proteins with similarity to eukaryotic D-serine dehydratases (DSD) and D-threonine aldolases (D-TA). DSD catalyzes the dehydration of D-serine to aminoacrylate, which is rapidly hydrolyzed to pyruvate and ammonia. D-TA reversibly catalyzes the aldol cleavage of D-threonine into glycine and acetaldehyde, and the synthesis of D-threonine from glycine and acetaldehyde. DSD and D-TA are fold type III PLP-dependent enzymes, similar to bacterial alanine racemase (AR), which contains an N-terminal PLP-binding TIM barrel domain and a C-terminal beta-sandwich domain. AR exists as homodimers with active sites that lie at the interface between the TIM barrel domain of one subunit and the beta-sandwich domain of the other subunit. Based on their similarity to AR, it is possible members of this family also form dimers in solution.
Statistics
?
PSSM-Id: 143487
View PSSM: cd06812
Aligned: 12 rows
Threshold Bit Score: 572.369
Threshold Setting Gi: 163797790
Created: 18-Jun-2008
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of the PLP cofactor and substrate analogs to other members of the superfamily.
  • Comment:The active site is composed of residues from both subunits of the dimer. There are two active sites in the functional dimer.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                            # #                    #                    
gi 115421763   9 LDSLDTPCLLLDEaRMEANIEYMKARMRrLGVTLRPHLKTPKSVEVARRAMQTpq-gPAAVSTLQEAEQFARAGVRDILY 87
gi 162428210  19 LDALQTPCLLLDEvRLRANVERMRAHLTrLGVAFRPHLKTAKSLDVARIAMTApe-gPAMVSTLREAEYFARGGVRDMIY 97
gi 91790640    6 FEPLETPALLLDTaRMDRNIERMRGRMTrHGVHFRPHVKTNKCMDVTQRLLDAga-rGITVSTLREAEYFSERGITDILY 84
gi 124875570   3 LHTLNTPAALIDVaRMQRNIARMQTHLDaLGVRFRPHVKTTKCRQVVDVQIAAga-qGITVSTLKEAEQFFAHGIRDIVY 81
gi 28870797    5 LPSLDTPVALLDMpRMQRNIQRMQQRMNeLGVRLRPHVKTSKSLPVIQAQMAAga-rGVTVSTLKEAEHCFAEGISDVFY 83
gi 50120966    7 LNALETPFLLIDEfRFQRNIDRLYQRTKnLGSQVRPHLKTLRSIEAGRYLLKDsa-sPATVSTLAEAEAFAGAGYTNLLY 85
gi 149909568  25 LAKIDTPFLLVDKaKFDTNVAFLRAKLApHNVILRPHLKTVKSIAGAKHVLEGfd-sPATVSTVKEAEAFADAGYTNLTY 103
gi 157961435  15 YQSLDTPFLSVDKqIVLKNLARLRNKIDgLGAELRPHFKTVRAIEAVEHLLPQki-sPITVSTVKEAEALVEEGYSNIVY 93
gi 85711442    3 WDALETPYLRVDRpRFDANIKRLNCHLSaLKTPLRPHVKTLKSLPAVKAIFNGgt-gPITVSTLKEADFFAEHGFLDQIY 81
gi 163797790   3 IRDLPTPHLVLDRrKLEHNLARMRGRASeLGVRLRPHGKTAKSAEVVRRAVGDgp-vMVTVSTLREAGYFAENGIRDIVH 81
gi 56460862    8 IAHPDTPYLLIDEaKLKSNINYLKQRVEsLGSHLRPHLKTLRTLEAAGYLLDSks-aPATVSTLAEAEAYAKAGYTDLLY 86
gi 197780628   4 LADPDTPFAVVDVhKVRRNVERLATRAErLGVTLRPHVKTAKSLDVAALLHAGdrpcPVTVSTLAEAEAFADGGYRDITY 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #                                                  #      #                     
gi 115421763  88 AVGLSPAKIDRVLALRAQGVNLTVVVDSV-EAAQALASRSRaagapIPALIEIDADGHRAGVRPeQQALLCAIGRAlheG 166
gi 162428210  98 GVGIAPAKLARVSAIRAGGADLTVILDSL-EQAEAVADHSSsssdpLPVLIEVDADGHRSGVAPgDADTLLAIGRLllaG 176
gi 91790640   85 AVCLSPNKLAHALRLRRQGIRLTVIVDSV-EMALQLNPERIqgddrLDVLIEIDCDGHRSGVQP-DSPALLAIAQAlqsQ 162
gi 124875570  82 AVDMVPAKLGQALALRRQGCDLKIVADSL-PAAHAIAAFGRehgerFDVWIEVDVDGHRSGIPP-DADLLIDVGRAltdG 159
gi 28870797   84 AVAIAPGKLDQALKLRRIGCRLSILTDSV-VAAQAIVAFGQqhdeqFQVWIEIDCDGHRSGLTV-EDNALIEVARTlveG 161
gi 50120966   86 AVGIAPHKLPRIANLIHKGVNLHILLDSV-EQARAVTDYALannvtFSVFIEIDCDGHRGGIPP-ESDALLELAQQidgN 163
gi 149909568 104 AVGITPQKLPRIQALLNKGINISILLDSV-AQAQAVTAFCQaqnikIPTLLEIDCDGHRGGIQP-DDTRLVEIGKIlhqG 181
gi 157961435  94 AVGISEGKLPRISQLIAKGAKVRVLLDTL-EQAQFVSQYSEqhccvIPAMIEVDCDGHRGGIHP-DDPLLIEIANIlhqG 171
gi 85711442   82 AVGIVQNKLPHVKRLIEQGVDLKIILESE-TQALQVVEFCRmhdivIPVLIEIDCDGSRAGLTA-ESPTLMDVSKIl-aQ 158
gi 163797790  82 ANCIAPNRLPEAAALLRDGVNLAITTDSPaVAAEIVRAGREfg-vrFAVMIEVDVGEHRGGVAA-ESDALLATARTlhdG 159
gi 56460862   87 AVGIAPAKLKRVAALRQQGINLHILLDNI-TQAQAVVDYAAefgqdFSVFIEIDSDDHRGGIKP-SDSKLLTIAKT---L 161
gi 197780628  84 AVGIDPHKLPRVVALLRRGVTLRVLLDSA-EQAEAVAEASRraglrVPTQIEIDCDGHRGGLEP-DAPGLPEIGGIl-hE 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #                                            ##               ####     
gi 115421763 167 GAELRGVMTHAGESYSSpDVVAIRDMAERERSAAVRAAAVLREQGLPCPVVSVGSTPTAMFAENLEGVTEVRAGVYVFFD 246
gi 162428210 177 GVSLRGVLLHAGDSYALsDPEALAAAAEAERRAAVTAAETLRAAGLPCPMVSVGSTPTARYARNLVGVTEVRAGVFMFGD 256
gi 91790640  163 GLAVGGVMTHAGDSYICrSTEAIVAMAEQERAGAVQAARRLRAAGHACPVVSVGSTPTALFAENLDGVTEVRAGVFVFFD 242
gi 124875570 160 GMLLGGVLAHAGSSYEYdTPEALAAIAEQERSRTVRAAERLRAAGLPCPVVSIGSTPTALAAERLDGVTEVRAGVYVLFD 239
gi 28870797  162 GMQLRGVMTHAGSSYDLdTPEALQALAEQERLLCVSAAERIRQAGLPCPEVSIGSTPTALSAQGLAGVTEVRAGVYVFFD 241
gi 50120966  164 GATLTGLLAHAGESYACrTDEAIRAAARAECAAINIAGRRVRALGIACPVLSVGATPTAHFAEDLTGITEVRAGVFTTFD 243
gi 149909568 182 GAELRGVLTHAGESYDCfNKAALVAAAEGERHAAVIAATQLIASGLPCPVVSVGSTPTAHFAENLDGVTEVRAGVYTFFD 261
gi 157961435 172 VASFHGVLAHAGESYLCfDPKSLAEAANNEVNMTLKAANNLRAAGIPCDIVSIGSTPTAHSYQDLNGITEVRAGVYGFFD 251
gi 85711442  159 AELFKGILLHAGGSYGCmTRSDKVAAAENERATAVYVKERLELAGIDCPMVSIGSTPTAHFAQDLTGVTEVRAGVYTFFD 238
gi 163797790 160 GMEVRGVLAHAGHTYGCtDAAAVAVIAEAERAGAVRAAQRVREAGIPCREVSVGSTPGALFAKHLNGVTEFRPGVYMFMD 239
gi 56460862  162 GEHFTGLMTHAGGSYACnTEQGLKNFAKQECDAVRIARNNLETAGIHCAITSVGSTPTAHFGEDFSDISEVRAGVYTTFD 241
gi 197780628 161 AGCLDGVLTHAGESYFArTAAEQRLAAENERDSAVAAAERLRAAGLTVSTVSVGSTPTAHAAEDLTGVTELRAGNYVFFD 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                             #                                          
gi 115421763 247 LFMAGLGVCKQDDIALSVLTTVIGHQPEKAWILVDAGWMAMSRDRGTANQAvDQYYGLVCDVAGRPLPGLVLKQTNQEQG 326
gi 162428210 257 LFQAGVGAVAMEDIALSVLATVIGHQRAKGWIITDAGWMALSRDRGTARQKvDQGYGVVCNLAGQPYPDLIVADTNQEHG 336
gi 91790640  243 LVMAGLGVCRTDEIALSVLTTVIGHQKDKGWTVTDAGWMAMSRDRGTSRQSlDQGYGVVCGVDGAPLEDLIMVDANQEHG 322
gi 124875570 240 LVMHNVGVCELSDIALSVLTTVIGHQEEKGWAIVDAGWMAMSRDRGTQRQArDFGYGQICTEAGDVLGDYLLSAANQEHG 319
gi 28870797  242 LVMHNIGVCGADELALSVLTTVIGHQQDKGWVIVDAGWMAMSRDRGTQRQRqDFGYGQVCTETGEWIDGARVTGANQEHG 321
gi 50120966  244 LVMKNVGVCSLDDIALSVVSTVIGHNREKGWVFIDAGWMALSRDRGTASQAkDYGYGLVCDLHGSPYHDLCVTVTNQEHG 323
gi 149909568 262 LVMAGIGVCKPDDIALSVVTSVIGHNEEKDWLFIDAGWMSLSQDRGTQSQRqDCGYGLVCDSEGNIIPGLQVIGANQEHG 341
gi 157961435 252 LVMAGIGVCQLKDIAASVVTTVIGHNREKGWIFIDAGWMALSLDRGTAGQPkDCGYGLVTDCNGAVLDGLQVISVNQEHG 331
gi 85711442  239 LVMANINVCSLDDIALSVVTTVIGVKPEKGWILIDAGWMALSRDRGTASQAvDYGYGMVLDGAGRPLTDWIVESTSQEHG 318
gi 163797790 240 LFQVGVGCCGIGEIALTVLGTVINHRPDEGTAHIDAGALALSKDRSTSAQPvDRGFGLVTDVFGLPLPGLSVEAVHQEHG 319
gi 56460862  242 LVMKNIGVCDFSHIAMSVVTTVIGHNKEKNWLLTDSGWMALSRDSGTAGQNrDFGYGQVCKIDGSVLDGLCVNSTSQEHG 321
gi 197780628 241 LVMAGLGVCRVEDLALSVVVTVIGHRPEYGWILTDGGWMAMSRDRGTAAQAqDQGYGLVTDLAGNPIPGLVMTAASQEHG 320
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1                                                                                  
gi 115421763 327 IITLRPGATgt----------lPDLPIGTRLRILPNHACATGAQHDAYQVL-------RGADVA-AVWPRFRGW 382
gi 162428210 337 IVALRRGSDaa----------lPDLPVGARVRILPNHACATGAQHDCYYVL-------DGSGRVgAVWPRINGW 393
gi 91790640  323 IIARRPGSTag---------psPFLPIGSQLRILPNHACATAAQFPCYQAL-------DGEQRLaGTWQRFYGW 380
gi 124875570 320 IVSRVGTPDtg---------iaQRFPIGTRLRILPNHACATGAQHPDYQAI-------GDDGAA-QTWPRFYGW 376
gi 28870797  322 IITLATGDStd---------itGRFPIGSRLRILPNHACATGAQFPDYHAC-------DAEGAV-HIWSRLHGW 378
gi 50120966  324 IIALPTDSGfs----------vDNFPVGTRLRILPNHACATAAMHQHYQVL-------KSHRHEqETWQRITGW 380
gi 149909568 342 IIAAVGSDIdritdgmndglrlSDIAVGTQLRILPNHACATAAMHAGYYVA-------DPVQIElDYWARIQGW 408
gi 157961435 332 IIGAVDNSDls----------lDNFPVGSRLHILPNHGCATASMHQHYHVF-------DTANETyEIWNRIQGW 388
gi 85711442  319 VIKQRAGSSei----------pSDVQIGDKLRILPNHACATASQFDKYYVA-------DAEGQLsEQWSRFNFW 375
gi 163797790 320 RLAGAGGVPf------------EILPIGSRVRVLPNHVCMTAAMYDRYAVVdgt--ekGDEQPVeAFWDRVNGW 379
gi 56460862  322 VIELSDAYQl------------EDFPVGHQLRIMPNHACATAAMHPVYHVL-------MSDGSH-NTWQRITGW 375
gi 197780628 321 TLTARDGTRl------------PELPLGTRLRILPNHACATAAQHHGYHVVdgtrtttAGAQVVqAFWSRVSGW 382

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap