Conserved Protein Domain Family
PLPDE_III_DSD_D-TA_like_3

?
cd06814: PLPDE_III_DSD_D-TA_like_3 
Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzymes Similar to D-Serine Dehydratase and D-Threonine Aldolase, Unknown Group 3
This subfamily is composed of uncharacterized bacterial proteins with similarity to eukaryotic D-serine dehydratases (DSD) and D-threonine aldolases (D-TA). DSD catalyzes the dehydration of D-serine to aminoacrylate, which is rapidly hydrolyzed to pyruvate and ammonia. D-TA reversibly catalyzes the aldol cleavage of D-threonine into glycine and acetaldehyde, and the synthesis of D-threonine from glycine and acetaldehyde. DSD and D-TA are fold type III PLP-dependent enzymes, similar to bacterial alanine racemase (AR), which contains an N-terminal PLP-binding TIM barrel domain and a C-terminal beta-sandwich domain. AR exists as homodimers with active sites that lie at the interface between the TIM barrel domain of one subunit and the beta-sandwich domain of the other subunit. Based on their similarity to AR, it is possible members of this family also form dimers in solution.
Statistics
?
PSSM-Id: 143489
View PSSM: cd06814
Aligned: 11 rows
Threshold Bit Score: 519.586
Threshold Setting Gi: 126738650
Created: 18-Jun-2008
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of the PLP cofactor and substrate analogs to other members of the superfamily.
  • Comment:The active site is composed of residues from both subunits of the dimer. There are two active sites in the functional dimer.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               # #                    #                 
gi 120553036  49 ALSEAGLATPTLVLDQGGLDYNLSALNSHLn-GEYHYRIVVKSLPSLPLLQRIMAaTGSNRLMVFHQPFLNTVAEALPqa 127
gi 87119640   47 ALDKAQQSKPSLVIDKSLLLENIKVLKQHIs-GRFDYRIVAKSLPSIPLLKLIMEtANTQKLMVFHQPFLNQVATELPhs 125
gi 146308950  39 LLQQEGNGIPALLLDLDRLDANADLLAERLn-GRLGLRLVSKSLASVGLLEYLAKrLQTQRFMVFHQPQLNRLARSFPqa 117
gi 126197166  20 ELKANGPMCPVLLIDLDRLDHNIDVVMRSVrrGGKHLRLVEKSLPSPGLLAYIARrAGTRRLMSFHQPFLNHDAVAFAda 99
gi 34496496   21 ALRQAGIAEPTLVLDRQRLRANIRRISDHLa-GKMALRVVVKSLPCPALLAEAALlAGSRRQMLFSLPQLEAMAKGER-- 97
gi 56708810   12 ALIAAGRQLPTLVVDTERLAANMAAVRRMTa-PSLALRVVEKSLPAPDLLRVVIAgLETNRLMSFHLPFVQQAAAAFPea 90
gi 13476781   11 ALKTAGIFQPCLLLDRDRVDGNIALVKARLd-PSLAVRLVDKSLACLPLLSHIGKkVGTDRFMTFHPPISAAVLKAFRda 89
gi 126738650  18 MLRQTGVTGPCLILDKGRLMHNLALARERLh-PGVEVRVVAKSLAAPPLLDLAMEqLDARGVMSFSAPMLEELLHLRPna 96
gi 119475637  43 LLKEKGPGRPVMMIDVDRMNHNIDQISNSVg-PEKTYRIVVKSLPSMDLLKHIMKrANTNALMVFHQPFLNAIVDAFPea 121
gi 94500657   44 ALKEQSIGTPRMLVDYDQLLQNVKTLTGHIk-PRFDYRIVAKSLPSIELLQVVMKaADTNRLMLFHQPFLSQVAKRLPda 122
gi 198262470  37 VYQSNGLSEPRLIIDGQRLDQNIDSLVQKI--GSDRYRIVAKSLPSLPLLRHVMArANTRKLMVFHRPFLSVIVREIPda 114
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                                                             #      #      
gi 120553036 128 DVLMGKPLPVAAVDRFYRQltsst-----sgfrPEQQLQWLVDSRQRLEAYEGFARSQQrrLRINLEIDIGLHRGGFTDt 202
gi 87119640  126 DVLLGKPLPVNAAAKFYQVferqpkkntqeqfqPERQLQWLIDSPHRLNQYQNLAHSLKinMLINIELDIGFHRGGVADd 205
gi 146308950 118 DLLLGKPMPVHAALNFYRQlpgh------igftPARQLTWLIDSAERLRQYAELARALGqpLRIALEIDIGMARGGFRDp 191
gi 126197166 100 DILLGKPLPVRSAELFYREhkg--------afdPARQLQWLIDTPQRLRQYLALAQGLGtrMRVNIELDVGLHRGGVADq 171
gi 34496496   98 DILLGKPLPAAAALAFYRQfsda-------afrPERQLQWLVDTPERLAQYRELARGQHlhLRLNFEIDVGLHRGGVADi 170
gi 56708810   91 DILLGKPMPVGAVAGTSPT--------------LDAQVHWLIDSPERLAEYAALAAQSDraLRICLEVDIGLHRGGFSLa 156
gi 13476781   90 DLLYGKPMPVGAARAALVKgd-----------aDWSRVCWLIDTQERLAEYGALADEIGteLRIAFEIDVGLHRGGFASp 158
gi 126738650  97 EHLLGKPLPAPAAARILDRnv-----------rARDQVIWLIDTLERAQQYDALARERGlvLPIAVELDVGLHRGGFTTk 165
gi 119475637 122 DILMGKPMPITAVATFYNTltrs-------aaaASQQIQWLIDTPERLIQYQQLANKLGvtMRINCELDVGLHRGGLSTa 194
gi 94500657  123 DILMGKPMPIQAVERFYQDgihn-------dfvAAQQIQWLVDSPQRLEQYREFAESNKlsMAINIELDVGLHRGGITRd 195
gi 198262470 115 DILLGKPMPIAAVKRFYKNltpe------qihsANRQIQWLVDGLEHLEEYRALAQSLNvtLRINLEIDIGLHRGGFKQg 188
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  #                                                   ##
gi 120553036 203 --gELARALTTIDh-sPWLSFSGFMGYEPHIMKAPgp----aaWLRDRAMARYRDYVSTAEQhlgr-sltDLTLNTGGST 274
gi 87119640  206 --iIFEKMLKQIDa-dPALSFSGLMGYEPHVAKVPgs----klALRDAAMQSYRDKLAIALKisgk-htdELTLNSAGSP 277
gi 146308950 192 --qALGQALQHLQd-pPQLQLQGLMGYDAHVAHAApwt--eqrRVFADTNARYREFLAAARAfpll-wprQPLLNGGGSL 265
gi 126197166 172 --aALGELLALIAahpQHLEFAGFMGYDPFVGMGVpgilgspeELFAKVMALYQGHVDFARQrfaalwtpQLTLNTAGSP 249
gi 34496496  171 --aTLRRMLDMAAe-ePRLQWSGLMGYDAHVAKIPdlpg-lraKALRHARARYAAFAAAARAhpaartasGPCFNAAGSP 246
gi 56708810  157 ragEFAAALTRLRd-dPNLHLTGLMGYEPHIARLPrlia-sraRALDAARAAYARFHALTVQtlgagtaaKLIYNTGGST 234
gi 13476781  159 --eTLSKALSALPa-hKRLRCEGVMAYEAHAPHIPglfg-gpaKALAQGSDRAAAFVACLGAd------qRRILNIGGSK 228
gi 126738650 166 ---TLPPAIEAISt-lKGLRLQGAMGYEPHLAKLPa-------LLRAAARRRVESGLKLAREmlqv-hcnTPIINTGGSL 233
gi 119475637 195 --qSLDAVLNIIDgdpEHLNFSGFMGYEPHLTGLKakl---esPSVQSVLSIYQAFIDQAKArgv--dteKLTLNGAGSH 267
gi 94500657  196 --sDLISMLDMIEt-sDTLNFSGFMGYEPHIGKVPgd----vlDHRDKAMAEYQHYVQLTEQhlkr-siqHLTLNAGGSP 267
gi 198262470 189 g-sDYPEALRYIHshtEYLALSGLMGYEPHIAKMPlasaermnKAKSKATAQYQWFKDYIKLnysdtyrtNLTFNGAGSP 267
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         ####                                                           
gi 120553036 275 TYQLYrglsGELPVNELSAGSGLVMPTDFDLPTLAEHRPAAFIATPVLKLLEtt-rIPGAPGLGRLQALWNPNRARTAFI 353
gi 87119640  278 TYRYYd--eGDYPHNEVAAGSCLVKPSDFDLDTLSDHEAAAFIATPVLKVMQdt-qIPGVDGLGRLMTIWNPNRSKTFYT 354
gi 146308950 266 TYPLHs--rSDTPLNEVAVGSALLKPGDFDTPALSEHQPALWIASPLLKVVDg--aLPYLGAVQPLMTAWNPNREHAYYL 341
gi 126197166 250 SYRMHe---KEMLSSEVSVGTAMLKPSHYDLASLAEHVPAVYIATPVLKSTGav-qIPALDGKSRLFSWWDPNQRGTFFI 325
gi 34496496  247 TYRLYh---GDGPENEVAVGSAMVKPADFDLPLLDDLLPACFIATPVLKTLPrfelPYGVEWLGGLVGGWDANARRGYFI 323
gi 56708810  235 TFMLYd---GSGPETELALASALVKPTDFDLPALEALQPAAFIAAPVLKTVErp-eIPMAPGLSRVLRLMGRSPERACFI 310
gi 13476781  229 TALLH----HGGIANEVSMGSAFVLPSDFDTAGLVGFQPAAFIAAPILKVVAp--mLPGPPAVTRLLQALGRFPRKGCYL 302
gi 126738650 234 TFDRYg---PQHGVSEVALGSLLVKPKDFGLPATEGFAPALFIATPVLKYMP----RNPIPGLEALSPLTGLRNRADLAI 306
gi 119475637 268 TLGIYe---NDNLMNDLSAGSGIVKPMDFDTFYMKGNYPALFIATPILKRYDel-kLPGDPAIAKLLPLWNPNMQRLYYI 343
gi 94500657  268 TYQYYn--qGQWPMNELSAGSCLVKPTDFDLASLQDHEPACYIASPVIKQLDqa-eIPGIDFMGELLSSWDPNLKQAFFI 344
gi 198262470 268 TCQLYq---SDDTINDISAGSSLLMPTDFDLLSIRDQKPALFIATPVIKALGal-eLPFLEPICSLLARYNPNWQRSYFI 343
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
Feature 1           #                                                                           
gi 120553036 354 YGGNWPATPVSPAGLtDNPVYgRSSNQEMLNFAdgldLAEGDRVFLRPHQSESVFLQFGDLAIFdpaRGAITHRWPVFR 432
gi 87119640  355 YGGNWKAHPISPKGLsINPVWgHSTNQEMLNGSehiqLNTDDWIFLRPTQSEFVFLQFGNLIIYd--QEGIIDSWSVLK 431
gi 146308950 342 YGGRWPAHPVSPAGLdYDALYgRSANQERLIGSaatgLRADDWVFLRPAISEGLLGGFGEMRLLr--AGQLVGRWSTLP 418
gi 126197166 326 YGGNWMADFASPEGLrSNGIYgRSSNQEMVNGSpsvgLEVDDQVFLRPTQSESVLLQFGDLLALr--GGRIVERWPVYA 402
gi 34496496  324 YGGNWLADPASPSGLeSSGLYgHSSNQQLLVGSgrqaLAVDDMVFFRPRQSEAVLQQFGDIAVYe--GGRIVASWPVLP 400
gi 56708810  311 YGGNWLADPCHPAGArRSALFgHSSNQEMYDLPanstLKPGDFLFFRPRQSEALFLQFGDIAVFd--GTAITDWWPVWH 387
gi 13476781  303 YGGKWMAEPVFPEGMrTNGLLgLSSNQQFMGLPvdsvARPGDYAFLRPTQSEAVLQQFGPIAVFs--GGRIIDRWPVLP 379
gi 126738650 307 YGGYWKASPVYPKDYgYSGIFgRSSNQEVWSGPrlkqSPVGGYAFLRPDQSEAILPEFGELLVVs--EQQELEHWPCFS 383
gi 119475637 344 YGGYWKAKIVSPAGV-ADPIY-QSTNQSPITTSnsvdLQIDDYIFLRPTQSEHVMLQFGDILVVk--GGEIIDNWPVFH 418
gi 94500657  345 YGGYWKAKPESPKGLkLNPVYgRSTNQEMLNGSdsvqLKQDDYVFLRPTQSESVMLQFGDLLVYr--KGQLIDHWPILK 421
gi 198262470 344 YGGYWKATPCSPQGLsNNGLYgRSSNQELLTGSgssqLKINDVVFLRPSQSEAVLLEFGEILVVr--NNEIAEQWPHLA 420

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap