2J66


Conserved Protein Domain Family
PLPDE_III_Btrk_like

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cd06839: PLPDE_III_Btrk_like 
Click on image for an interactive view with Cn3D
Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzyme Btrk Decarboxylase
This subfamily is composed of Bacillus circulans BtrK decarboxylase and similar proteins. These proteins are fold type III PLP-dependent enzymes that contain an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain, similar to bacterial alanine racemases, eukaryotic ornithine decarboxylases and diaminopimelate decarboxylases. BtrK is presumed to function as a PLP-dependent decarboxylase involved in the biosynthesis of the aminoglycoside antibiotic butirosin. Homodimer formation and the presence of the PLP cofactor may be required for catalytic activity.
Statistics
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PSSM-Id: 143506
View PSSM: cd06839
Aligned: 22 rows
Threshold Bit Score: 464.76
Threshold Setting Gi: 83594465
Created: 16-Jun-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the structures of PLP and substrate bound to ornithine and diaminopimelate decarboxylases.
  • Comment:The active site is composed of residues from both subunits of the dimer. There are two active sites in the functional dimer.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                            # #                     #                   
2J66_A        11 LTKRFETPFYLYDGDFIEAHYRQLRSRTNpaIQFYLSLKANNNIHLAKLFRQWG---LGVEVASAGELALARHAGfSAEN 87
gi 145593239  10 RPDAPQTPAYFYDLDELTRSNAALRAALPrpTELFYSLKANPHPTVVAELTAAG---CRAEVCSPGELRVALAAGcDPAQ 86
gi 159038615  18 LRSGVQTPAYVYDLAEVRANAVRLRAALPerSEIYYSLKANPHPAILDTLREQG---ARAEVCSVGELDESLAAGwPPDT 94
gi 41386599    7 LADQYGTPLYVYDLDRVAEARHDLRTSLPdeVEIYYALKANPHPQVAGALRSGEgreCRAEISSVGELAAALTAGfRASE 86
gi 118725495   9 IVNKYGTPVYAYDTEKIMSQYALLKNSLPqeIGIFYSMKANPLLGICQLFKKLG---SGIETASSGELHTALCAGfPPEK 85
gi 118725894   9 IAHKYKTPLYLFDGDRLEETYFNMKKVLPdaFEFFYSVKANPSYGICRILQKCS---SGIEVASAGELHLALEAGfDNKK 85
gi 78223226   20 LAARYGTPFYLYDLDKVKEKCAAVRKAFGdsLELLYAVKANPNRELLAAMRGEV---DGLDIASAGELERALETGyDAGC 96
gi 159036153   7 LAEEYGTPLFVYEADTVAAQYTGLRQRLHpaMDIFYSLKANPNLALCGHLRSLG---AGAEVSSLLELTTAREAGvPPGD 83
gi 161385580  16 IATTAGTPFYLYDASVLRGRLDALRAALPq-VDFFYSLKSNPNLSVTRVLHGHG---AGCEVSSLLELETSLQAGaTPER 91
gi 69936913   15 AAARFGTPAYVYATDVVEERLAHLRAHLGrwFAVSFAVKSNPNPALLGWMRDRL---DYLDISSGGELALGREAGwDAAR 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                                              #                             
2J66_A        88 IIFSGPGKKrSELEIAVQSgiYCIIAESvEELFYIEELaekeNKTARVAIRINPDKs--fGSTAIKMGGVPRQFGMDESM 165
gi 145593239  87 VLYTGPGKRdIDLAEAVRAgvELFSVDSpYGLDQLDRMarehDVEVRALVRINDDTp--aPGQGLTMTGVASQFGADVRW 164
gi 159038615  95 LLYTGPGKRdQDLRHALRRgvREFSVDSpIGLDQLDRLageaGVQARCLLRINDDQp--vPGQGLTMTGVASQFGVDVSW 172
gi 41386599   87 ILYTGPGKTdGELDEAIGKgvKTFCVESlTDLQHVGAValrhGVVADCLLRINSATa--sATTSIRMTGTPSQFGIDSET 164
gi 118725495  86 IIFTSPGKTyDELKYAVETgiYSINIESlEEAEVINEIadkkGRVVDISIRINPDFd--iAGSGIKMSGVASQFGIDQIR 163
gi 118725894  86 ILFTGPGKTySELEFAVDSkiLAINAESyKEIMLIDDIakakNKIVDVGLRIHPNFkiesKNPAISMMGTGTQFGVDIDE 165
gi 78223226   97 ISFAGPGKTrDELRRSLEAgvGCISVESlRELNDLRDLvragSHRAYILVRVNPQLl--iRDFAVKMGGKASQFGIDEEE 174
gi 159036153  84 IVFPGPGKSrEEITACLDAqiYAIICESfEELELVNDLarerGVRARVALRVNPAFa--vKGSGLTMGGKPRQFGIDEAH 161
gi 161385580  92 ILMVGPGKSeTELARAIELgiKAIVVESaHELTQIDALarqqGRVQNIALRVNPDFh--aGGAKLNMSGRPTQFGIDQSE 169
gi 69936913   92 ISFTGPAKReAELRAAIEAglGELVLESlREARLADAVaaemGRVQPVLVRLAPARv--pKGFGDQMAGRPSPFGVDVED 169
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              #  #                                       ##             
2J66_A       166 LDAVMDAVRsl-qFTKFIGIHVYTGTQnLNTDSIIESMKYTVDLGRNIYEry---giVCECINLGGGFGVPYFsHEKALD 241
gi 145593239 165 VLDQPDLFGpr-rNVCPVGFHLYMGSNvDGEDALIAQFTQSVDTIRRLVAat---grQPELIDLGGGFGAPFArAGARPD 240
gi 159038615 173 VFADPARFTgg-lNAPVVGLHLYMGTNmTEVHDLLAQFGRSLATAAQLRCalarhgvRLRVVDLGGGFGAPFAcVGEPVV 251
gi 41386599  165 LVDAMPELRav-pGTRITGLHFFPLSNaRDEASLIGEFRHTIAYAAGLAEet---glTLEFLDIGGGFAHPYGaPGERPV 240
gi 118725495 164 LDSVLNELKal-qNLNIMGIHIYTGTQaLNAHSIVQSMEQIVKLALDTSEky---vfKLKFLNLGGGFGVPYFkDDSTLD 239
gi 118725894 166 IPEILKYIKss-kNLNLVCFHVYAGSQiFQPDTTLAYFEQTINIFKELIEkn---dlHIKVLDFGGGFGVSYDgKNKPYD 241
gi 78223226  175 LPSALDFIKanadAFDFKGIHIYAGTQcLNEEALAQNLANTLAIAARITTdy---gmECRVINIGGGLGVSYYeEHPGLD 251
gi 159036153 162 LFALPGLRLa---HLRVVGVHVYLGTRiLDETVIAENTRRILELAERLAAhl---dfPLEFVDVGGGLGVAYFdGERDLA 235
gi 161385580 170 LPDVLKQAEsc-aHLRLCGLHAYMGTRiLTHETVVANVGNILNLATEVIStl---kaPLDFVDVGGGFGIPYYdGETELD 245
gi 69936913  170 AAEILPQIAal-pNLRIIGLHIYSGTQcLKPEAICENWRIFMQVFRDTCAal---dlRPEKLIFGAGLGIPYHpGDSALD 245
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                    ####                                                
2J66_A       242 IGKITRTVSDYVqea-rdtRFPQTTFIIESGRYLLAQAAVYVTEVLYRKASKGEVFVIVDGGMHHHAASTFrg-rsMRSN 319
gi 145593239 241 LPKLADRLAALLadafpgwPEQGPAVAFESGRYLVGTCGTLVTRVLEVKRSQGIPVVVLESGIHHLGGMSG-----LRRL 315
gi 159038615 252 LSGLRDGLYRLLdaelpgwRDGAVRVVFESGRYLVGTAGTLVTRVLDVKRSHGRDLVVLESGINHLGGMSG-----LRRL 326
gi 41386599  241 YRELRTELAAALdehfphwREGAPRIAVETGRYQTSGAGTLLTRVVNIKVSRGRKFVVIDAGINTFGGMSG-----LGRL 315
gi 118725495 240 MDILRNEFSEMWnky--kdRLFNTQVFVESGRFLMAESGTYLTKILYRKECKGRKYLVCDGGSNQHAASAFlg-rhIRNN 316
gi 118725894 242 FEYFGAEINKLYhky--edFFQGKKLVFESGRLLLAESGVFLTEVQYRKVLHDKVFLITDGGMNHNSLSTFre-kkIRGN 318
gi 78223226  252 LDKLALLFRAEFdry-reaTGTKPRILLELGRFLVAEAGIYVTRVVSEKLSRGEQFYVLDGGMNHHLSASGnlgmtIRKN 330
gi 159036153 236 VERLTDLVNPVVadf--hdRHRDTRLIMELGRYLVAPAGTYLIRVRYVKSSMGERFAVTDGGTHQHMAAVGig-sfVKRD 312
gi 161385580 246 LDALGQAVTPLVqsf--gaTHPKTRVVIELGRYLSGPSGQFVTRVQQTKSSKGEHFAVCDGGSNVHVAAAGqg--fLRKN 321
gi 69936913  246 LAAIAAEIGPELdafraepRLADARLVLELGRYLVGPAGWFVTRVLSVKQSRGTRIAICDGGLNDNLAASGnfgmvLRRN 325
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
Feature 1                                    ##                           #   #    #           
2J66_A       320 YPMEYIpvreds--grrelEKVTIAGPLCTPEDCLGKDVHVPaLYPGDLVCVLNSGAYGLSFSPvHFLGHPTPIEILK 395
gi 145593239 316 PPIVPDlltvda-aagppiPGTIVTGPLCTPLDTWARATALPpLVPGQLVAVPNVGAYGLYASLvAFLAHPMPIEVTI 392
gi 159038615 327 PPLNPHllsdt---aagdlRPTMVAGPLCTPLDLWSRNAPLSpLHPGDTLQVPNVGAYGLSASLlAFLSHPTPTEVAV 401
gi 41386599  316 LPVAVEpeytgsaeateltDVASLAGPLCTPGDILGREIRLPeLAPGDLVTIPNAGAYGVTASLlMFLGRPAPVEVVL 393
gi 118725495 317 FPIQILnks-------sneEQVNVVGPLCTPTDVIGQNVMLPeAVPGDIVAVLKSGAYGVTQSPgLFLSHPLPAEVQY 387
gi 118725894 319 FLMKILdnr-------neeETVTVAGPLCTPEDILGRNVTLNkADRGDILCIKNTGAYGSSFSPlGFLGHPAPCEVLI 389
gi 78223226  331 YLVRNLsrpg------aerIICTLVGPLCTPLDLMGKGVTVEvPEVGDLVGFLNSGSYGYSASPlLFLGHGEPCELLV 402
gi 159036153 313 FPLRLLgrpt-----dgplVRWTVTGPLCTPNDTLVRAAELPdLRRGDLLGVLRSGAYGPSASPtLFLGHGHPAEVLV 385
gi 161385580 322 FPIRLLrdgkae-iddeaaQPWTLTGPLCTPQDVIGKSVLMAtPQVGDLISIGQSGAYGPTASPvNFLGFGAPAEVMI 398
gi 69936913  326 YVMHRVggded---gtrpeEKQDISGPLCTSIDKLGGGVALPrLEEGDLIAIHACGAYGPTASPlHFISHPMPAEVLL 400

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