Conserved Protein Domain Family
PLPDE_III_MccE_like

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cd06841: PLPDE_III_MccE_like 
Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzyme MccE
This subfamily is composed of uncharacterized proteins with similarity to Escherichia coli MccE, a hypothetical protein that is homologous to eukaryotic ornithine decarboxylase (ODC) and diaminopimelate decarboxylase (DapDC). ODC and DapDC are fold type III PLP-dependent enzymes that contain an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain, similar to bacterial alanine racemases. ODC participates in the formation of putrescine by catalyzing the decarboxylation of ornithine, the first step in polyamine biosynthesis. DapDC participates in the last step of lysine biosynthesis, the conversion of meso-2,6-diaminoheptanedioate to L-lysine. Most members of this subfamily share the same domain architecture as ODC and DapDC. A few members, including Escherichia coli MccE, contain an additional acetyltransferase domain at the C-terminus.
Statistics
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PSSM-Id: 143508
View PSSM: cd06841
Aligned: 19 rows
Threshold Bit Score: 419.36
Threshold Setting Gi: 120586900
Created: 19-Jun-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
Conserved Features/SitesClick to see Conserved Features Help
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the structures of PLP and substrate bound to ornithine and diaminopimelate decarboxylases.
  • Comment:The active site is composed of residues from both subunits of the dimer. There are two active sites in the functional dimer.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                  # #                  #                
gi 165975419  21 GIESGDNSFVIFDVDgalERLEHYRCQLKSF---FPNSSIAYSYKSNNLAQWCQIISGKGLYAEVCSVDEMNLAKRDGF- 96
gi 150399162  11 LNEDFGDSFYLLDSN---KFVKNYDEFLGEFqkiYPNTVIAYSYKTNYLPHLCSIVNKKGGFAEVVSKMEYDLAIKIGVi 87
gi 150010400   8 LIKSNGTPFYLFDEK---GFIDNYRKLEGIFkaiYPNYQLSYSYKTNYTPFVCQLVRSLGGYAEVVSDMEYTLAKKLGYd 84
gi 160885676  15 LRKEYGDAFYLLDSG---QFRNNFIELKDTFrkiYPNFNIAYSYKTNYTPKFCKIVNELGGYAEVVSEMELEIALRVGVe 91
gi 150006522   2 QLSNLKTPCFILDSD---KLVSNVLEFKRALnsrFSNHCIGYSIKTNSLPYMLHMLNELGCYAEVVSYTEYALALQVGFe 78
gi 90579305    9 LSEVYGDSYYLLDSK---KFEDNFECLLDAFnsiYSNTKIGYSYKTNYIPKLCKIIDIKGGYAEVVSDMEYDLAIKLGVe 85
gi 168822845  20 RPSHSDRPFFIFDAD---SALERARHLTAACkeyFPDAVIAVSVKSCSLGIFLRLIAEEGLSAEVCSADEFKLALKAGFt 96
gi 78214012   13 IRDSETGPFYLIDFD---GIENSIMRLKSAFdadFKKVRLAYSFKTNSLQAITTFMHSKSMGAEVVSGQELEWALSDNFe 89
gi 121602208  20 RPGHTDFPFFLFDAD---NALAKAKTLISASkkyFTDSNIAVAIKSCSLGIFCKLLAEEGLNAKVCSADEFQIALFAGFp 96
gi 108760390  59 LVERWGSPLHVVHMA---ALRRNAERFLAVPpgrTGGCEVYYSYKTNPVPGVLSFLHGLGVGAEVISAYEMWLALKLGVp 135
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #                                               #                         
gi 165975419  97 -NRIVFDGPLKKtSELLKAIEIG-ALIEVDNiDECKRLNELCKLh-kLTCRIHLRLSHYYDdn----------lSRFGLS 163
gi 150399162  88 pSNIIVNGPLKTkEHLEYFLLKG-SIVNVDSyRELDLIREIANSnpgKILSVGIRCNFEMGst----------vSRFGFD 156
gi 150010400  85 nKQIVYNGPSKG-EEMFDHLEHQ-GILNIDSlDEAKRIISYVKGhpdRNYTVGIRINMHVGgd---------fiSRFGLV 153
gi 160885676  92 sKRIIWNGPIKNaKKLEEFLVAG-GTDNIDSvEELAVVKAIAERhpdKVLNLGIRCNYDVQdg---------vvSRFGLD 161
gi 150006522  79 kSHIVYNGPAKDkETFLDAIKNG-AYVNIDTkREIEWLNDLNKQ---HSYKVGIRVNLNLGkispedakegesdSRFGFS 154
gi 90579305   86 pERIIVNGPYKPkNILEKFLLNK-SIVNLDSfVEIEYLKEIALKnptNMIEFGIRCNFDIHnn---------vvSRFGID 155
gi 168822845  97 gDRIILDGPYKNsEDLSLALDKG-ALVHIDSaHELSEIIGLMSGy-nQKIGVGVRLSHIYSdt---------qrSRFGVT 165
gi 78214012   90 pNNIYFDGPVKSkKEHKRAAELN-VNIQVDSvQELIDLLEITSDp--SKLKVGARLSMPYKnk---------inSRFGLT 157
gi 121602208  97 nDRIILGGPLKTsEDLSFALEKD-ALIYVDSvSELRKLNTLAVNk-nRRYGVGIRLSHFYRng---------kySRFGIT 165
gi 108760390 136 pERIVYNGPVKSeASVREAISRGiQLLAANHaEEIAVFARLAAEl-dRRPRVAVRVTTNSGw-----------sAQFGTP 203
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                     #  #                                     ##        
gi 165975419 164 es-eAINLLEMLISKs---eYLILDGFHLHVGSNl---pNAEKICKAVIQYHELIlry--mpDDGTLNLGSGIPADSfsa 234
gi 150399162 157 vrdaEFIEKFKELKKi---dNLYIKGIHCHFPDR-----NLDSYSKRVDNLINILdllfkenIPEFVDIGGGYFGKVndf 228
gi 150010400 154 p---ESKELQETVSLiketvNLNLIGLHCHISRSr----GVEAWTKRTEIMLKAAddy-idgVPAYISLGSGMYADMpef 225
gi 160885676 162 vdseDFQQVLEFVITt---kNVHFINFQCHFAKR-----QIEYWSARAKGMVELIdri--giIPERLDVGGGLFGKMads 231
gi 150006522 155 f---ENGELEEAINKiqlckNVKLGGLHLHRTSLt---rSLNVYRNICKYAIRIInsl--glELDYIDVGGGYFGDKpn- 225
gi 90579305  156 i---NNPDLLKKLKEiseikNVKFVGIHCHYPDR-----NADSYSVRAKKIIEVAssl-fttPPKYIDIGGGFFGKMder 226
gi 168822845 166 a--eEFRDEIVPLLTsc--pDISLRGFHLHTGSNl---eNPSKVSDCLRDWLPFLven--mpEGGHLDMGSGFPADSfsp 236
gi 78214012  158 r--sEFKEATTIMKRt----GISFSGLHLHSGSNi---tNINQYRESIMSCIDCItet--whELSWIDFGGGFPANTala 226
gi 121602208 166 ke-eYINDILPILSNt---dHLYLQGFHLYVGSNp---eNYPKITDTLHHWLPFLveh--mpSSGHLDISTDFLTNPep- 235
gi 108760390 204 i---AGGAALRAYQQargskHLDVVGLHAHRGETirtegDFTAFVESVLDFTDTLhqe-lglDLEVLDLGGSLNTPTveh 279
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                               ####                     
gi 165975419 235 ss------------------dnptPCPEVFFSSIYDTIkncfg--tvcdKWNYIFEPGRHLVEDFGYFIGKVISTKNRyG 294
gi 150399162 229 lak---------------qfncnvPSYSDYASIIASRFkeyfknipenrKPKLILEPGTALVADTMKFIAKIVEIKNIrG 293
gi 150010400 226 lkqq--------------fggdtvPTYEDYADCTIQPFadhyk--dvenQPILFTEPGTTVVARYLSFVTKVLSVKEVrG 289
gi 160885676 232 lka---------------qfkndiPDYQAYAEATATIFadyfa--dknvKPELLIEPGSAVVGDCMKFVGTVKTIKCVrG 294
gi 150006522 226 -----------------------aPTYKEYVDTIYSSLleeg---idvsKMKVIVEPGNAIVASPFSFLSEIIDTKKIgD 279
gi 90579305  227 lkk----------------qfssyHDYKEYANNIATEFkraysalpkeeQPILFLEPGSALVADTMKFVCRVIDIKNIrG 290
gi 168822845 237 va------------------avptVEPAAFFRDIVSVLseydp--aliqKWKLIFEPGRTLSEDHGYAIGKTVSVKNRyD 296
gi 78214012  227 ne------------------seelPTPEAFSSCIKSIIskkn---lslnNKTVVIEPGRCLVEDNGLLLARVEQIKKReD 285
gi 121602208 236 ----------------------enYDPETIFRSIHDVLnsydp--nlskRWKLIFELDHYLNKDSSYVIGKALGYKNHyD 291
gi 108760390 280 iaerdwrlnltffrdvpapdpaasLSIDRYVALAVEMVethya-rrgrqRPRIFLEPGRAMTGDTQLLLGRVHALKAGeD 358
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       ##                             # 
gi 165975419 295 VKVAQTNIGINWIPSi-RNWDHSFTLFhnhnhisddksdEYIIAGFNCFEcDCLFPSVILPs--NLSDYLFSVRGCGAYD 371
gi 150399162 294 KKIAMTTGSKFNMGLfgLSINLPINVYsefkn--gkfinNVDISGYTCIEaDYLFKNYCGI---LNIGDYIAFDNVGSYS 368
gi 150010400 290 RYIANMDGDYHNLGEicTMKKLPVTVLsggke--qhtytNMDIMGFTCLEqDVLYPGFEQS---IAEGDILVFDNVGGYS 364
gi 160885676 295 KWMATVLGSQKNISM--SGINPPMEVItmgge--qkeykDLDFVGFTCIEgDVLYNNYNGK---LAHEDAIVISNCGSYS 367
gi 150006522 280 SQIIVTDGSRNDVDPf-FLKKDYFKSFirkqee-kvfidSQMVVGCSCLEyDKLFTLQNQPl--LNVGDRILYQNVGAYT 355
gi 90579305  291 TNIAMTSGSKFNIGLlsSTVNMPLDVHskyin---dkvnEYDISGFTCIEsDYLFKGYTGC---LFQGDFLIFNNVGSYS 364
gi 168822845 297 SEVIQTNLGINWIPSv-HNWHHSLLPLghneh--ipddtTQILAGFNCFEnDCLFPRGPLN---LKKNQLFIIRGCGAYD 370
gi 78214012  286 RNIVVTNAGLNLVKSm-HSWYHPVIFLrqeid--eeqstPYEVYGCNCFEsDLIIDELEGPpsdLRIGDWIAIGSTGGYD 362
gi 121602208 292 AKIIQTNLSINWITSi-HNWHHSLTLLndgdk--dstqdKQILAGFNCFEdDSLSPQGYYG---LTEGQHFLIRSCSNYN 365
gi 108760390 359 RTWAVLDAGINHAECvrNEYHQLFHVNrpda----padkVYTVVGPICTPgDTLYHAKRLPd--LRVGDTLAIMDAGAYF 432
                        410       420
                 ....*....|....*....|.
Feature 1          #   #              
gi 165975419 372 MQTGnQWTRNLYAVYTITNDV 392
gi 150399162 369 FVFKpPFICPNVPIIDYNGDK 389
gi 150010400 365 IVSKpQFIKPQSAMYVLRTNG 385
gi 160885676 368 LVMKpPFILPNFPVLDICGEK 388
gi 150006522 356 MTLSpLFIRFFPRVYLKTLNG 376
gi 90579305  365 IVFKpPFILPNVPIIELTGNS 385
gi 168822845 371 LQTAnEWTRTRPPVYALLNQE 391
gi 78214012  363 MQNFnAWTQPYPSIYGSSREG 383
gi 121602208 366 MQASnEWTRKKPPVYIWLRGS 386
gi 108760390 433 VPFAtSFSYPQPAIVALEDGK 453

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