Conserved Protein Domain Family
GT_GPT_like

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cd06851: GT_GPT_like 
This family includes eukaryotic UDP-GlcNAc:dolichol-P GlcNAc-1-P transferase (GPT) and archaeal GPT-like glycosyltransferases. Eukaryotic GPT catalyzes the transfer of GlcNAc-1-P from UDP-GlcNAc to dolichol-P to form GlcNAc-P-P-dolichol. The reaction is the first step in the assembly of dolichol-linked oligosaccharide intermediates and is essential for eukaryotic N-linked glycosylation. GPT activity has been identified in all eukaryotic cells examined to date. Evidence for the existence of the N-glycosylation pathway in archaea has emerged and genes responsible for the pathway have been identified. A glycosyl transferase gene Mv1751 in M. voltae encodes for the enzyme that carries out the first step in the pathway, the attachment of GlcNAc to a dolichol lipid carrier in the membrane. A lethal mutation in the alg7 (GPT) gene in Saccharomyces cerevisiae was successfully complemented with Mv1751, the archaeal gene, indicating eukaryotic and archaeal enzymes may use the same substrates and are evolutionarily closer than the bacterial enzyme, which uses a different substrate.
Statistics
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PSSM-Id: 133461
View PSSM: cd06851
Aligned: 3 rows
Threshold Bit Score: 264.746
Threshold Setting Gi: 167043919
Created: 23-Jul-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Mg++ bindingputative
Feature 1:Mg++ binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                  ##    
gi 156357179  36 AGKDMNKKDnkdPIPEGLGAVGGALFLICMFlfiplpflsiwlekgdfdfphhefvmFIAALLSICCMIFLGFADDVldl 115
gi 167043919  36 TVKDVNKKGn-vMIPRPGGISILIGIVASEItlyff----------------fpiseILAIIITSVLGFSVGIIDDRktm 98
gi 116754254  30 LGWDMHKPGr-pKVPEMGGVPLVFGFVLGVLvyigietfy---------lnsyrytpILAALCTVLMACIIGIMDDIlgw 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 156357179 116 ----kwRDKLILPTMASLPllmvyfvnigvttiivpkpvrfifgfdldlgilyyvymGMLAVFCTNAINILAGINGVEAG 191
gi 167043919  99 ----ggWFKPVALAFCAAPilllgaydsnldfpl---------fgsvkipslylalvVFMIPITGNTFNSIDVLNGVVSG 165
gi 116754254 100 kaglrqWQKPMFMLFAAMPmmvinaghttmslpl--------igrvdwgilyplviiPIGVVGASNAFNMVAGYNGLEAG 171
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 156357179 192 QSLIIAMsivlfnllelqgtccwqnhlfslyfmlpfiaVCSALLYYNWYPSSIFVGDTFCYFAGMAFAVVGILGHfsktm 271
gi 167043919 166 FTTIASFalaislfvqqny--------eigiaclclafASLAFYKYHKLPSRIFPGDSGAVMFGATYGVIAIVGEvevia 237
gi 116754254 172 MGVIIFAalgyaglvmgkt--------saaalsiimlgSLLAFLYFNRYPARVFPGDTMTYSVGALAACVAILGDiekia 243
                        250
                 ....*....|...
Feature 1                     
gi 156357179 272 LLFFIPQVFNFVF 284
gi 167043919 238 AVAILPAIINSFL 250
gi 116754254 244 VTLFIPYAIDFFM 256

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