Conserved Protein Domain Family
GT_GPT_archaea

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cd06856: GT_GPT_archaea 
UDP-GlcNAc:dolichol-P GlcNAc-1-P transferase (GPT)-like proteins in archaea. Eukaryotic GPT catalyzes the transfer of GlcNAc-1-P from UDP-GlcNAc to dolichol-P to form GlcNAc-P-P-dolichol. The reaction is the first step in the assembly of dolichol-linked oligosaccharide intermediates and is essential for eukaryotic N-linked glycosylation. Evidence for the existence of the N-glycosylation pathway in archaea has emerged and genes responsible for the pathway have been identified. A glycosyl transferase gene Mv1751 in M. voltae encodes for the enzyme that carries out the first step in the pathway, the attachment of GlcNAc to a dolichol lipid carrier in the membrane. A lethal mutation in the alg7 (GPT) gene in Saccharomyces cerevisiae was successfully complemented with Mv1751, the archaea gene, indicating that eukaryotic and archaeal enzymes may use the same substrates and are evolutionarily closer than the bacterial enzyme, which uses a different substrate.
Statistics
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PSSM-Id: 133466
View PSSM: cd06856
Aligned: 23 rows
Threshold Bit Score: 194.389
Threshold Setting Gi: 18314106
Created: 5-Nov-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Mg++ bindingputative
Feature 1:Mg++ binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                        ##              
gi 116754254  30 LGWDMHKPGrpKVPEMGGVPLVFGFVLGVLvyigietfylnsyrytpILAALCTVLMACIIGIMDDIlgwkaglrqWQKP 109
gi 18202470   34 VGKDINKPDkpEIPLLGGIGIIAGFIAGSFsllltd-----vrservIPAVILSSLLIAFLGLLDDIfnv----rqSVRA 104
gi 197623012   1 MGKDMNKYDhpEIAEAGGIGVIIGITIGIFiylflkalfgsdthlaeIYATLSAVILAGLIGFSDDIlgwkegipqRYKP 80
gi 197622948  33 WRDDPNKPYkrEVLTGGGIPVLFALTIVYValgvky-----sqhslfFFGLLITTFMGSFFGFIDDVrdv----kiWKKV 103
gi 21228198   42 VAKDVYKKGtpTIADRGGTAILLIAMLSLSmnslff------kftstNYVAMIVIALFGLFGVLDDMini----grSSKL 111
gi 167043919  36 TVKDVNKKGnvMIPRPGGISILIGIVASEItlyff-------fpiseILAIIITSVLGFSVGIIDDRktm----ggWFKP 104
gi 18314106   32 TSRDVYKGId-GVPRAGGLIAVAASAVGYGvaal---------vaeeAAVVLVISLIIGLLGLLDDLrgv----seYVRV 97
gi 118576894  35 SVRDVNKPGrvMVARPGGISIMAGILAAEAvlyaa-------fqsveVLAITATTAGAFAAGLLDDRrvm----sgWFKP 103
gi 119719329  38 RRPDAHKPGnpLVAHSGGVILFLGFLLSFSlliafggl--gfsekvkLLAVLATSALCFAVGLLDDKlvl----kgVLKT 111
gi 159041547  28 LVPDVHKPNkpLIPKLGGLVVLAYVAVSFTllsisrd----ltnsmyYVVFYTTPIVLGVIGIVDDFthv----heFLRV 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 116754254 110 MFMLFAAMPMMVInaghttmslp-----ligrvdwgilYPLVIIPIGVVGASNAFNMVAGYNGLEAGMGVIIFAALGyag 184
gi 18202470  105 FLPIFASVPLIVYsvghsiisip-----flgpinfgifYYIIIIPFALTITSNAFNMLEGLNGLGVGMGIIMLSALAyig 179
gi 197623012  81 ILTTILALPFMTLtlihpyynsf------eswrvplwmYSLLFVPIAIIGTSNAINMVAGYNGLEAGLSSIILLTMAika 154
gi 197622948 104 PIMLIPPIPFVIInilnplwnnt-----tvlwinwgllYWILIIPVVYMGFSNGANIIAGYDGLEVGIYILILLLYIfig 178
gi 21228198  112 LIMYYCSYPLIQYathtavtlps------vgdlelgilYLQFIVPTYVLVASNLVNMHSGFNGLSSGLAILVLVSLIiks 185
gi 167043919 105 VALAFCAAPILLLgaydsnldfp------lfgsvkipsLYLALVVFMIPITGNTFNSIDVLNGVVSGFTTIASFALAisl 178
gi 18314106   98 LVPVFLAIGLARAvgqmkltv---------pmvglfygATGWFAALALPVMTNAFNMLDPVNGYLPAANIIIGLALAava 168
gi 118576894 104 LALAAASAPIIVLgaydphlsfp------lfgsvhipiLYLAVAALMIPITGNTINSIDVLNGVSSGFMVIAGAALTisl 177
gi 119719329 112 FLTALSIIPILVValtypaaidwgrpvvpiigrmrmtvIYWVLFPISIAGSANVVNMLDVLNGIVPGTALVAFAALAvig 191
gi 159041547 100 GASVLYPLIILLAmggklgylnf-----piighfsnpvVIIVLTIAVYGVFSNAINMMDVVNGVVPYSMILTSIIPLays 174
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 116754254 185 lvmg--ktsaaaLSIIMLGSLLAFLYFNRYPARVFPGDTMTYSVGALAACVAILGDiekiaVTLFIPYAIDFFMQARGRf 262
gi 18202470  180 lthtgptyqaglIALSAIFSLSAFLIFNKYPAKIFPGNVGTYFIGALIGAIGIAGFmytalAILYIPYVVEFILKLRTNf 259
gi 197623012 155 ysms--elwisyMALLAVAALIGFLAFNWYPAKVFPGDSLTYPIGTYIGALAILGNmelfgALLFPLYYTKLILYIKAKk 232
gi 197622948 179 iiln--nslvlhISIPLMFALLAFELYNLYPSKVLLGNVGSFPIGGLLGIIPFVGHfeivlPIVFLPHLVEFLFKVKYKg 256
gi 21228198  186 vlis--dveniiSVVAITGATIGFYLYEQYPARIFWGNVGSLTVGAAIGTIIVIQGfiisgFIMLIPHTVNFLMYVYWKi 263
gi 167043919 179 fvqq--nyeigiACLCLAFASLAFYKYHKLPSRIFPGDSGAVMFGATYGVIAIVGEveviaAVAILPAIINSFLFLSSVk 256
gi 18314106  169 virg--qadaafLLAIHVAASLALFLYNKYPAKTFNGNVGSYFLGASISTIAVLYDlvaylILTGLPFVINGALIIFSSg 246
gi 118576894 178 viig--nyemaaVSMPLPLVALAFYRYHKNPSRIFPGDSGALALGAMYGAIALAGGveiiaAVALLPAVINSFLFLSSMr 255
gi 119719329 192 alsg--dglllvIALVAVAILLAYYPFNAYPARVFNGDSGSLFIGGLLGAIAVVFHlefvvLTLLLPHILNGFFVVVSFr 269
gi 159041547 175 imtn--nitllqMTLTLLIPSIILFAFNKYPARLFNGNGGTYSIGALVAGLMLYSGlgtliLLVTVPYVINGLLIVVSSr 252
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                              
gi 116754254 263 gkea-------fanlGDDGSLSQSGIyhlthlai------allrrlkGRVYERDVVLFMWGVEAVVAMLS 319
gi 18202470  260 kgvs-------fgkvDSSGRLYWDEKphslth----------ivmkmGRFKEYQVVIILWGMEAIFAVIA 312
gi 197623012 233 idkvgd--ieefgvpDKNNHLKMPNKkiygvthla----iliqkkirGFATERGVVITLLSIQTLISLIV 296
gi 197622948 257 htsv-------fgivDENGIIRNKDGiksvlh----------wliswGNMTEKKITLAMLGIESILCIVA 309
gi 21228198  264 kkyph----vkfgkvREDGTLEVPNPltlkw-----------vlpyyYRVTEKQATYAMFLLTSVFCIMG 318
gi 167043919 257 rivehre-ikkptlhADDFKLKSTDEnhapitl-------vrllvakKPLSEKQICNEILKLTMFSGVLA 318
gi 18314106  247 gikgre--kiqrptvLKNGAVYQECNspilslv--------rlavadKPLTEYEIFKTLVILTAITSALT 306
gi 118576894 256 rivehrqikarpvetTDDFHLRATREkgapvtl-------vrlilarGPMKEAEVARSIFLLAAFSGILA 318
gi 119719329 270 glrehre-vkarpirVENGILKASDDpkapltlt------rlilsvgGPMTEKEVAKVYIAVEVVAAVLA 332
gi 159041547 253 giksre--klkpstyMNDFIIYPNLDkgstltlmkmivtdepgderkVIRGFYMVFNVATALTILLFIVL 320

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