2RAK


Conserved Protein Domain Family
PX_SNX9_18_like

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cd06862: PX_SNX9_18_like 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology domain of Sorting Nexins 9 and 18
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily consists of SNX9, SNX18, and similar proteins. They contain an N-terminal Src Homology 3 (SH3) domain, a PX domain, and a C-terminal Bin/Amphiphysin/Rvs (BAR) domain. SNX9 is localized to plasma membrane endocytic sites and acts primarily in clathrin-mediated endocytosis, while SNX18 is localized to peripheral endosomal structures, and acts in a trafficking pathway that is clathrin-independent but relies on AP-1 and PACS1.
Statistics
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PSSM-Id: 132772
View PSSM: cd06862
Aligned: 13 rows
Threshold Bit Score: 197.924
Threshold Setting Gi: 190588795
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Structure:2RAK_A; Human SNX9 PX domain binds Phosphatidylinositol-3-Phosphate; defined at 3.5A contacts.
    View structure with Cn3D
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                    ###                  
2RAK_A         47 FDCVVADPRkg-------------------sKMYGLKSYIEYQLTPTn----tNRSVNHRYKHFDWLYERLLVKFGsaIP 103
gi 215273942  276 FQCTIDDPTkq-------------------tKFKGMKSYISYKLVPTh----tQVPVHRRYKHFDWLYARLAEKFPv-IS 331
gi 74944671   219 YSVVVASPKke-------------------sKFKGMKTFIAYQLTPSf----nNISVSRRYKHFDWLHERLVDKFCl-IP 274
gi 156408492  189 FTCSVTEPEkk-------------------sKFKGMKSFISYNVVPTn----tMRPVSRRFKHFDWLYNRLVDKYTl-IA 244
gi 32565953   227 YTCIVDKPKke-------------------sKLKGLKSFIAYSITSSl----tNIQVSRRYKHFDWLHEQLSAKYVl-IP 282
gi 167519731  129 LSGHVWQPSqepftlev------saqegkesKFGGLKKFTAYQICNTt----tQATVMRRFKHFDWLHQRLVELYPc-IV 197
gi 190588795  159 KHIIVVNKNgltwkpaknectyrigstetdsKYAGAKTYTTYKIHPIhg---dDEAIRRRYKQFDWLYGRLVARFPt-IA 234
gi 167530871  357 GHITIEHCGar-------------------sKYKGLKSYEAFLIKGAv----pGTEAERRHKHFQWLHDRLVDKYSc-LC 412
gi 219425587  240 YKVTVADPKkd-------------------sKLKGLKSFITYQLTPDn----tNQTVSRRYKHFDWLYERLIEKFTt-IA 295
gi 198423762  219 YSCSIENPKqq-------------------sKMKGLKKFVCYNLTPSd----tAVTVQRRYKHFDWLYARLVERYPm-VA 274
gi 219471316  173 FSVEIKNPTka-------------------sKLKGMKSFIAYEVTPTh----tNRSVTRRYKHFDWLYNRLVEKYSv-IS 228
gi 198420867  263 FTCTVTNPVke-------------------sKFSGLKSFIAYTITPSh----tDVAVLRRFKHFDWLYEQLVRKYGvsIS 319
gi 71014102  1006 YHVTSTYPLgdq-----------------sdAVSGSQGCIPYDPLGGphpagaQVTVLRRFTQFEWLHQVLAKHFSa-LL 1067
                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
Feature 1               #             #                                                    
2RAK_A        104 IPSLPDKQVTGRFEEEFIKMRMERLQAWMTRMCRHPVIS-ESEVFQQFLNFR---DEKEWKTGKRKAERDELA 172
gi 215273942  332 VPHLPEKQATGRFEEDFISKRRKGLIWWMNHMASHPVLA-QCDVFQHFLTCPsstDEKAWKQGKRKAEKDEMV 403
gi 74944671   275 VPPLPDKQISGRYEEQFVEHRRVQLQEFVDWVCRHPVIS-KCEVWYHFLTCR---DEKIWKSGKRKAERDPYM 343
gi 156408492  245 IPPLPDKQITGRFGEDFVEKRREKLEKWVGRLCQHPVLS-KSPVVNHFLTCAd--SEKEWKAGKRKAENDKFT 314
gi 32565953   283 IPPLPEKQVAGRYEEDLIDHRKHILQLWVNKICRHPVLS-QSEVWLHFISCT---DEKDWKNGKRRAEKDEYI 351
gi 167519731  198 IPPLPEKQVSGRFSKNFVEYRRQKLERMLNRVALHPVLG-ASSVFRHFLTAT---DKKDWKSGKRQAEGEAKS 266
gi 190588795  235 IPKLPEKRIRGRFSEDIIQMRKELLETWLNRLARHPIIN-ESQVFSGFLKKQ---DQIEWKEFKRRMEKDPGN 303
gi 167530871  413 VPPMPGKEYESKFNENHVKKREERLEAWINRVARHPVLGaDVLALKHFLTCSt-gDATRWKQGKRDAEKDRFL 484
gi 219425587  296 VPPLPEKQVTGRYEENFVEGRRQQLQLWVDRMSCHPVVA-QSEVFQFFLQSP---SDKTWKDGKRRAEKDPLV 364
gi 198423762  275 IPRLPDKQASGRFEEEFIEGRMARLKLWISYICQHPVLS-RAQIFRNFLTLT---EQKAWKMSKRQAEKDDLV 343
gi 219471316  229 IPPLPDKQVTGRYEEEFIQHRMAQLQLWMNRMTRHSIVS-RADVFQHFLSCGd--DKNTWKAGKRRAERDNLI 298
gi 198420867  320 VPQLPDKQITGRYEDDFIEGRRVQLQTWLCRIALHPVIS-CSDVFGHFVTSKd--DEKAWKQGKRKAEKDEAV 389
gi 71014102  1068 IHPVPEKQYSGRFASDFIETRRADLEMWMSRLVRHPVLR-YSEPLRFFLSCE---DEVDWRSYAANLLRYGCS 1136

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