Conserved Protein Domain Family
PX_Atg24p

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cd06863: PX_Atg24p 
The phosphoinositide binding Phox Homology domain of yeast Atg24p, an autophagic degradation protein
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. The yeast Atg24p is a sorting nexin (SNX) which is involved in membrane fusion events at the vacuolar surface during pexophagy. This is facilitated via binding of Atg24p to phosphatidylinositol 3-phosphate (PI3P) through its PX domain. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.
Statistics
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PSSM-Id: 132773
View PSSM: cd06863
Aligned: 14 rows
Threshold Bit Score: 167.465
Threshold Setting Gi: 6322424
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                ###     
gi 70906407   98 LITAVRDPVKEla----------------eTKDAYVSYLVSAKTNLpif--------------stpSPSSRRRYQDFKFL 147
gi 129716035  72 LECRVDSPLKEnd----------------gTKDAYISYLVTTHTDFksf--------------qkpDFTVRRRFTDFYFL 121
gi 71019673  108 LMVQVLEPRKEle----------------gQKDAFISYGIRAETNLahf--------------srtYMAARRRFNDFTFL 157
gi 164663149  15 LRVLVLDPRKEthgaqqqnrtndthapttrGSQTFVSYGVRAETNLphf--------------srtYMITRKRFQDFVFL 80
gi 19112676    2 LKCTIKNEQIEtl----------------rSGDTFVSYEIETESDLpvf--------------edkKFSVRRRYKDFEML 51
gi 50405569  117 INVVVTSPLRDld----------------sSSKPYISYLLTTATNHpsvmklstv--kkeqgkevvEITARRRYGDFRFL 178
gi 190348412  76 TTTEVTSPTRDtd----------------aAQKPFVTYLVTTVTNDpsiirlcstlkkvsedndmqSVAVRRRYGDFKFL 139
gi 213403232   3 FICRVADPQFEkp---------------lsGGNVYISYLILTKTELhtg--------------qenELHARRRFRDFTLL 53
gi 6322424    28 FEIIVSDPQKRtgd--------------pgSSSGYVSYQISTKTNNtsfydn--------rgdpesIIVVHRRYSDLLLL 85
gi 50305459   17 LSVLVSDPQKQk-----------------tHSQTYITYQVSTKIDGkd-----------------ePNCVVRRYNDFVLL 62
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
Feature 1                          ##                 #                              
gi 70906407  148 REHLVRDFPACVVPALPDKHRLEYITGDRFSPEFMERRRLDLHRFLERIARHpILQRSTLLRAFFEST 215
gi 129716035 122 YKTLYREYPACAVPPLPDKHKMEYVRGDRFGPEFTTRRAWSLHRFLKRLTLHpVLRRAPLLTIFLESP 189
gi 71019673  158 REGLKRDFPACVVAPLPDKHRLEYLTGDRFSAEFIERRTQDLQLFLERICRHpTLQRSQLLRSFLEST 225
gi 164663149  81 HDALVSDFPACIVPPLPGKHRIGYLTGDRFSSEFIMRRCMELQLFLERVCRHpLLQRAKILQQFLESN 148
gi 19112676   52 HNILSHDYNGYAIPPLPRKYTVSSFSGGSLSPIFIARRMQSLQTFLDRCSTHpVISNSMHMYQFLENN 119
gi 50405569  179 FDCLSNDHPEVMMPPLPSKLNFKYLTGDTFSTEFVHKRLHSLDRFVRFITCHkVLSQLSIFHLFVSDS 246
gi 190348412 140 HECLTADYPQHLVPPLPSKSNFKYLTGDTFHEEFITKRLQSLNRFIHYTNQHqYLSQSSIFKLFVSDS 207
gi 213403232  54 HELLRREYPLHFIPPIPRKHGVKQITGDAFSPDFIPRRLSSLQRFLDRCLEHpVLKSSLHLYQFLEVS 121
gi 6322424    86 HDILLNRFPTCIIPPLPDKKVFQYIAGDRFSQRFTQKRCHSLQNFLRRVSLHpDLSQSKVFKTFLVSK 153
gi 50305459   63 HQILINDHPALLVPPLPDKKVLNYLSGDRFSHSFTQKRCRSLQTFMRRLLSHsELSKSRILETFLTST 130

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