Conserved Protein Domain Family
PX_SNX8_Mvp1p_like

?
cd06866: PX_SNX8_Mvp1p_like 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 8 and yeast Mvp1p
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX8 and the yeast counterpart Mvp1p are involved in sorting and delivery of late-Golgi proteins, such as carboxypeptidase Y, to vacuoles.
Statistics
?
PSSM-Id: 132776
View PSSM: cd06866
Aligned: 22 rows
Threshold Bit Score: 151.612
Threshold Setting Gi: 19115109
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                              ###                       #
gi 23943858    75 DTVQVELIPEKKGLFL-KHVEYEVSSQr------------------fKSSVYRRYNDFVVFQEMLLHKFPYRMVPALPPK 135
gi 189041715  412 ENILVTLMPEKEGVFMfQHHNYEVSSIr------------------rGSKVVRRYSDFVWLLDCLHKRYPFRVLPLLPPK 473
gi 167526355 1149 SEFVQVMLGHATGMLK-KHTNYEVFSKr------------------kSTTVSRRFSDFEPLDAYLRKRYPYRIIPQLPPK 1209
gi 50543118   227 DSVTLAIVPEREGMFLfRHVNYSISSVsg----------------tdRITVIRRYSDFSWLQDYLLKKYCFRQVPLLPPK 290
gi 190408243  130 DIIIIEEIPEREGLLF-KHANYLVKHLialps----------tspseERTVVRRYSDFLWLREILLKRYPFRMIPELPPK 198
gi 71003942   304 DQVTVRLRSELEGFII-KHNVYIVSSSl------------------rKSQVTRRYSDWLWLAECLVKRYPFRCLPVLPPK 364
gi 170087768  241 ETVKVTILGHQGFILN-RYLVYEVTTEr-------------------GGSVPRRYSEFVFLWECLTRRYPFRLFPALPPK 300
gi 45185674   143 NIVVVEEIPEREGILF-KHTNYLVKHLvvlpn----------tnpssNQTVIRRYSDFNWLQEVLLKKYPFRMIPELPPK 211
gi 58268554   211 FRSTDPMVDNAEDRWW-KDQERIVVTLipekegwflqkyriesdkrgEGPVARRYSDFVWLMDVLEKRYPFRILPPLPPK 289
gi 19115109   285 LEMNVDIDLEPSGYFFyRHNNYIISDSs------------------nTREVLRRYSDFFWLHSYLMKKYPFRRVPLIPLK 346
                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
Feature 1         #                   #                              
gi 23943858   136 RMlg--------aDREFIEARRRALKRFVNLVARHPLFSEDVVLKLFLSFS 178
gi 189041715  474 RVafngn-hlsndGASFIEKRRRGLARFLNALVRHPVLGQEQLVIMFLTVP 523
gi 167526355 1210 TFggg------nmKPEFIEHRRRGLQRYLMFVGRHPVLQDDEILTAFLTDS 1254
gi 50543118   291 RLavngh--ylssDNYFLERRRRGLTRFINQVLRHPVLGQDEAVRTFVTLR 339
gi 190408243  199 RIgsq------naDQLFLKKRRIGLSRFINLVMKHPKLSNDDLVLTFLTVR 243
gi 71003942   365 RIampiagrhlsaDDLFIERRRRGLERFLRMLTCHPMLREDKLVEVFFTEP 415
gi 170087768  301 RIg---------aDEQFLEQRKRGLSRALSFVINHPVIKEDGLLAVFLTEP 342
gi 45185674   212 KIgaq------naDPIFLARRRKGLCRFINLVIMHPVLKQDDLVLTFLTVP 256
gi 58268554   290 RIn---------pSSAFLEARRLALIRLLSFLTAHPVLRTDACLNIFLTSS 331
gi 19115109   347 KFhsk-----cfnSKQFFRTPPPGLSDFVNDLSHHPIFSNDEVVRVFFTEP 392

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap