Conserved Protein Domain Family
PX_UP2_fungi

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cd06869: PX_UP2_fungi 
The phosphoinositide binding Phox Homology domain of uncharacterized fungal proteins
The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to PI-enriched membranes. Members in this subfamily are uncharacterized fungal proteins containing a PX domain. PX domain harboring proteins have been implicated in highly diverse functions such as cell signaling, vesicular trafficking, protein sorting, lipid modification, cell polarity and division, activation of T and B cells, and cell survival. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction.
Statistics
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PSSM-Id: 132779
View PSSM: cd06869
Aligned: 19 rows
Threshold Bit Score: 86.5675
Threshold Setting Gi: 6325354
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
putative
Feature 1:putative phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                     ### 
gi 74626312   167 NILEAYVLGVRQSTQAkml-------------------------rtkRVYEYLIKVSYEEn---------TFFIARKYSD 212
gi 71005354   220 SGFDINVVSVRNVITKgrl-------------------------rsrAHEEFIIRTRRGYgq-------pDIYVSRRYGD 267
gi 50545665   296 NGLCVNVVGVREIRTKrmlsf---------------------lagdhVSAEYLIETKMEShe------grTVVVARTFHD 348
gi 169861085  314 DYFEVNIVGVRTVVDKgrv-------------------------rskAHEEFIIRTRRAGy--------hDTYVSRRYGD 360
gi 46125967   205 NGWDVNVAGVRVTQVKhti-------------------------rkhKHAEFILRIKKKGe--------lEHFVGRRYSD 251
gi 164656831  192 FTTKVNIVCVRRTLSRgri-------------------------rhkTLDDFIIATHLNNd---------EIYVARRYKD 237
gi 68473478   189 GSNGLNIVAVRVIYENptnisswi----------------pfynrikVYHLFIVELQNDNq--------dTYYIAKSYQN 244
gi 150864801  187 HNLSINIVALRRTGAAvagtpsnsisswsp----mrfvtssrpsaprHHYEFVIQVTTREsvdgn-fqykSHFIARHYHE 261
gi 149236617  174 NDIYINIVAVRTVEHEqlrswf--------------------kvqrqHNYEFVIQIVLRQgke-----cvNVFVNKYYGQ 228
gi 199432431  200 NDLNLNIVAVKHIKEQngsgsdsagasrwnifkevsrlspynsnpvrHNYEFVIQFTKREtdkegtyhytSYFIPRPYHD 279
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  ##                                                     
gi 74626312   213 FSHFHHLLLKSYPN---AYVPRLPPKDDhdtylnssedstlsplp----------------------------------- 254
gi 71005354   268 FERLADSLKVEYPD---EGIRPPPPKDRsntdvadpyrrepasssqpatplpdg---------------------sdaac 323
gi 50545665   349 FKELAKKLHREFPG---RGLPNLPVRNRaasqatssgiggfvdtenddpede------------------------lnda 401
gi 169861085  361 FRTLANELAKRYPE---EDIKPPPAKDRsyvtapatpqtqsfseqlydaatgslgsaas-----------ylfddgshpa 426
gi 46125967   252 FAKLHSRLRTELPG---RVVPEMPKKNKsdttttiaftsmfsngndsdassissvstrpngytsmlspghrkkdssasfr 328
gi 164656831  238 FRQLHQQLRALFPT---EDLPDLPPKDMsstevqgslqpdsn-------------------------------------- 276
gi 68473478   245 FKTLHNKLKKKFPGl-fTEIHLPSHKDKhdngweinndkesla------------------------------------- 286
gi 150864801  262 FKTLESKLKRHLPGvmsTEVSELPRKFSndngyndiahekdtese----------------------------------- 306
gi 149236617  229 FRTLENELKKELPGamtTKIATLPHKEKhdegedld-------------------------------------------- 264
gi 199432431  280 FKKLEHDLKKKYPGlmsTDVPKLPQKLKrddgvlkkrdfessqslprsdsfdtle-------------------ndnhdd 340
                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                                  #                              
gi 74626312   255 ----srssdtndpqsdsqhvLRQERMRIELRQYIKNILNDDELHLTEEVLSFLTDD 306
gi 71005354   324 srastesnssqtripiptgpLAREKNRLTLRAYLRSLLASPSVAESALLMDFLTAE 379
gi 50545665   402 eehiddddtanvhhdgkrvsLPREKQRTTLRAYLHTLVSNRMVANSKTLLEFLYMD 457
gi 169861085  427 spvtpasshfpqspppnpakLAREKNRLTLRSYLHSLMSSSAIASSPVLKSFLLSG 482
gi 46125967   329 snrskglnsprlpsprqtivLWRENQRISLRAFLRSLLHNPQFANTNAMQEFLSGE 384
gi 164656831  277 -------eewtdltavtpnsLVRETNRLTLRAFLRSILAVPSVADSEIFQEFLTGD 325
gi 68473478   287 -----yeeeedttscsnekiLIREKMRLSLRGYLKSLMKIPEVAHCTILSEFLTTN 337
gi 150864801  307 ----laslsstvngsskttkLVREKLRLSLRGYLRTLSKYPEIVHSDIFLDFIDHG 358
gi 149236617  265 -------------mdgksgvLTRERLRLALRGYLRELIKVKEIVKTNAFQLFILTD 307
gi 199432431  341 ssfktasssnstsatpepqsLAREKLRLALRGYLNNLIKHNEIIHSDVFHHFVFNP 396

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