Conserved Protein Domain Family
PX_MONaKA

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cd06871: PX_MONaKA 
The phosphoinositide binding Phox Homology domain of Modulator of Na,K-ATPase
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. MONaKA (Modulator of Na,K-ATPase) binds the plasma membrane ion transporter, Na,K-ATPase, and modulates its enzymatic and ion pump activities. It modulates brain Na,K-ATPase and may be involved in regulating electrical excitability and synaptic transmission. MONaKA contains an N-terminal PX domain and a C-terminal catalytic kinase domain. The PX domain interacts with PIs and plays a role in targeting proteins to PI-enriched membranes.
Statistics
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PSSM-Id: 132781
View PSSM: cd06871
Aligned: 12 rows
Threshold Bit Score: 204.131
Threshold Setting Gi: 47210342
Created: 10-Jul-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                   ###                    ##            #
gi 83288430    13 LLDDTVPLTAAVEASQsLQSHTEYIIRVQRGIsAENSWQIVRRYSDFDLLNNSLQITGLSLPLPPKKLIGNMDREFIAER 92
gi 147906170   13 LLDDTVPLSAVIEASQsLHSHTEYIVRVQRGVsMENSWQIIRRYSDFDMLNNSLQISGLSLPLPPKKLIGNMDRQFIAER 92
gi 118096952    1 MLDETVPLPAQIEASQsLHCHTEYIIRVQRGVsAENSWQIVRRYSDFDLLNNSLQISSLSLPLPPKKLIGNMEREFIAER 80
gi 189239381  786 VLDDTEPLSCVIENWRnVNGHTEFVIKVQRGPfSDKTWRVYKRYNDFYKLHAYLQTSGISLQLPPKKLIGNMDPEFITER 865
gi 130485777   17 PLDDTVPLSAVIEASQsLQSHTEYIIRVQRGVsTDNSWTVIRRYSDFDMLNNSLLISGLNLPLPPKKLLGNMDREFIAER 96
gi 190589693    9 ECDDTIPLTCSIEKANiIDSHVEYVLIVQRGSnPENFWKVQRRYNDFVTLHAALQISGRDLPLPPKKLFGNLDKDFVAKR 88
gi 156404246   10 ALDDTVPLMCIFEGIS-KKEHVDYIIKVQRGAsPEGSWQVVHRYSDFAALHAALEVSGIEMPLPPKKVFGNMDTTFVVER 88
gi 66499854    13 LLDDTEKLTSVIENARtIDGHTEYVIRTQRGPlSEKFWRVSRRYNDFVQLNAALSISGIDLAFPPKKIIGNMEPDFIAQR 92
gi 24586453    13 PIDDTQALSCEITAVQeVAGHTEYLLRVWRGAsNKNYWTVLRRYNDFDRLDKSLRVSGIELPLPRKRIFGNMRPEFIAER 92
gi 170038328   13 VLDDTEAIACHIETAQnIDGHTEYVLRVQRGPyPENSWRILRRYNDFASLNKCLQISGIELSFPGKKFIGNMRPEFIAER 92
gi 198432907   11 VIDDTELLTCNITGTEtIQSHTEFIIRVQRGPlKENSWQLRRRYNDFVVLHETLKIAGVDLPLPPKKLLGNMEREFIAAR 90
gi 47210342    13 LLDDTVPLTAVIEASQnAQSHTEYIIRVQRGVsAENSWQVIRRYSDFDLLNNSLMVCGISLLLPPKKLIGNMDRDFIAED 92
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
Feature 1                                                                        
gi 83288430    93 QRGLQNYLNVIMANh-----------------------VLSNCELLKKFLDPNNYSANYTEIA 132
gi 147906170   93 QKGLQNYLNVITSNh-----------------------ILCNYEQVKKFFDPTNYSANYTEIA 132
gi 118096952   81 QKGLQAYLDVITSHh-----------------------LLSNCELVKKFLDPNSYSVNYTEIA 120
gi 189239381  866 QQGLQKYLNSVLMNp-----------------------ILVSSLPARSFVDPANYCQPFGELA 905
gi 130485777   97 QKGLQAYLNYITQHh-----------------------ILCSCELVKKFLDTNNYSANYTEIA 136
gi 190589693   89 KQQLQVYVNVILEDp-----------------------FLSHCLTVKKFFDPTRYTIDFYDIA 128
gi 156404246   89 QQGLQNFMNFILGNp-----------------------LLARHISVKKFLDPVNYSENFQENA 128
gi 66499854    93 QIALQNYLNNILMNp-----------------------ILASSLPMKKFLDPDNYTAPLHEIA 132
gi 24586453    93 KQALQEYINAVLMNp-----------------------ILASSLPAKRFVDPESYSQSFHDHA 132
gi 170038328   93 LSALQEYLNQVLMNp-----------------------ILASSLPTKKFIDPDSYSTPFHDLA 132
gi 198432907   91 QNGLQVLLESVLSHp-----------------------GLAACDTVKKFLDPNNYNFNQTELS 130
gi 47210342    93 RGDYRPTWTQLPSIpcfpapclsksswiptatqpitqkSPCSKSQCSSGQTSNGKSWSLSETW 155

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